Article
Oncology
Peggie Chien, Haibin Xi, April D. Pyle
Summary: This review describes the current state of human pluripotent stem cells (hPSCs) in recapitulating human myogenesis ex vivo, and discusses the potential of differentiating hPSCs into muscle stem cells for regenerative medicine, as well as considerations of stem cell and progenitor cell state and function.
EXPERIMENTAL CELL RESEARCH
(2022)
Article
Cell Biology
Jie Ren, Chaoyi Li, Mengfei Zhang, Huakun Wang, Yali Xie, Yu Tang
Summary: Efforts have been made to establish efficient methods for neural differentiation from human pluripotent stem cells, resulting in rapid generation of neural progenitor cells and motor neurons. Differentiation protocols involving retinoic acid induction and dual-SMAD pathway inhibition were compared for their effectiveness in generating specific neural subtypes.
Article
Cell Biology
Alexandra Neaverson, Malin H. L. Andersson, Osama A. Arshad, Luke Foulser, Mary Goodwin-Trotman, Adam Hunter, Ben Newman, Minal Patel, Charlotte Roth, Tristan Thwaites, Helena Kilpinen, Matthew E. Hurles, Andrew Day, Sebastian S. Gerety
Summary: Efficient and effective methods for converting human induced pluripotent stem cells into differentiated derivatives are critical for performing robust, large-scale studies of development and disease modelling, and for providing a source of cells for regenerative medicine. Here, we describe a 14-day neural differentiation protocol which allows for the scalable, simultaneous differentiation of multiple iPSC lines into cortical neural stem cells.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Ema Onode, Takuya Uemura, Kiyohito Takamatsu, Takuya Yokoi, Kosuke Shintani, Shunpei Hama, Yusuke Miyashima, Mitsuhiro Okada, Hiroaki Nakamura
Summary: This study found that nerve conduits coated with human induced pluripotent stem cell (hiPSC)-derived neurospheres can promote nerve regeneration and functional recovery in repairing rat sciatic nerve defects. This nerve regeneration approach shows promise as a new clinical therapy for treating peripheral nerve defects.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Yue Su, Ling Wang, Zhiqiang Fan, Ying Liu, Jiaqi Zhu, Deborah Kaback, Julia Oudiz, Tayler Patrick, Siu Pok Yee, Xiuchun (Cindy) Tian, Irina Polejaeva, Young Tang
Summary: This study successfully generated biPSCs with long-term self-renewal capacity and pluripotent characteristics from bovine mesenchymal stem cells. The cells exhibited morphological changes under different culture conditions, and transcriptome analysis showed high similarity to bovine ESCs and embryos. The naive-like biPSCs also demonstrated the ability to integrate into extra-embryonic tissues.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
June Baik, Carolina Ortiz-Cordero, Alessandro Magli, Karim Azzag, Sarah B. B. Crist, Aline Yamashita, James Kiley, Sridhar Selvaraj, Ricardo Mondragon-Gonzalez, Elizabeth Perrin, John P. P. Maufort, Jody L. L. Janecek, Rachael M. M. Lee, Laura Hocum Stone, Parthasarathy Rangarajan, Sabarinathan Ramachandran, Melanie L. L. Graham, Rita C. R. Perlingeiro
Summary: Pluripotent stem cells can be used to produce tissue-specific derivatives for clinical applications. Non-human primates are ideal preclinical models to study the delivery, biodistribution, and immune response. This study successfully generated non-human primate iPS cell lines and differentiated them into myogenic progenitors, which showed potential for therapeutic use.
Article
Food Science & Technology
Wei Wang, Tong Wang, Yu Gao, Geyu Liang, Yuepu Pu, Juan Zhang
Summary: Lead (Pb) exposure has detrimental effects on multiple human systems, specifically the central nervous system (CNS). This study used human induced pluripotent stem cells (hiPSCs) differentiated into neural progenitor cells (NPCs) to investigate the neurotoxic effects of Pb. The results showed that Pb exposure disrupted the cell cycle, induced apoptosis, and inhibited differentiation in hiPSCs, embryoid bodies (EBs), and NPCs. Whole exome sequencing revealed numerous single nucleotide variants (SNVs) and common deleterious mutations caused by Pb. Additionally, analysis of the Online Mendelian Inheritance in Man database identified genes related to CNS disorders in hiPSCs, EBs, and NPCs. This in vitro model has the potential to supplement animal models for studying neurodevelopmental toxicity.
FOOD AND CHEMICAL TOXICOLOGY
(2023)
Article
Multidisciplinary Sciences
Panetha Pansri, Phetcharat Phanthong, Nopparat Suthprasertporn, Yindee Kitiyanant, Alisa Tubsuwan, Andras Dinnyes, Julianna Kobolak, Narisorn Kitiyanant
Summary: The study found that BDNF can promote cell proliferation of AD-derived hNPCs, but does not have a promoting effect on neurite outgrowth. The JAK2 inhibitor AG490 partially decreased BDNF-induced cell proliferation.
Article
Cell & Tissue Engineering
Kenji Kitajima, Minako Shingai, Hikaru Ando, Mako Hamasaki, Takahiko Hara
Summary: Researchers developed a novel method for hematopoietic differentiation of human-induced pluripotent stem cells to address the issue of low production of CD38(+) hematopoietic progenitor cells. They found that the lack of FLT3 expression was responsible for this problem and solved it by adding interferon-gamma.
Article
Plant Sciences
Aeyung Kim, Seo-Young Lee, Sun-Ku Chung
Summary: The study found that caffeic acid (CAA) selectively eliminates undifferentiated induced pluripotent stem cells (iPSCs) and suppresses teratoma formation, suggesting its potential in preparing safe iPSC-based therapeutic cells.
Article
Biochemistry & Molecular Biology
Bing-Chun Liu, Fang-Yuan Liu, Xin-Yue Gao, Yang-Lin Chen, Qiao-Qiao Meng, Yong-Li Song, Xi-He Li, Si-Qin Bao
Summary: The study reveals that Wnt signaling plays a crucial role in driving human pluripotent stem cells towards neural stem cells differentiation and maintaining their self-renewal. CLNSCs exhibit spinal cord properties and high proliferative capacity, providing a new cell model for investigating neurogenesis mechanisms and potential applications in regenerative medicine.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Medicine, General & Internal
Chang Liu, Xing Feng, Guoping Li, Priyanka Gokulnath, Junjie Xiao
Summary: Human pluripotent stem cell (hPSC) technology provides abundant opportunities for disease modeling. Cardiomyocytes derived from hPSCs are efficient tools for studying cardiac diseases, drug screening, and pathological mechanisms. This review discusses the advantages and limitations of the 2D hPSC-cardiomyocyte system and introduces recent developments in 3D culture platforms derived from hPSCs.
Article
Chemistry, Multidisciplinary
Kangli Cui, Yujuan Zhu, Yang Shi, Tingwei Chen, Hui Wang, Yaqiong Guo, Pengwei Deng, Haitao Liu, Xiaoguang Shao, Jianhua Qin
Summary: The study reports the generation of a novel trophoblast-like tissue model from human pluripotent stem cells, which can reflect key features of early human placental development.
Article
Medicine, Research & Experimental
Edit Gara, Eleonora Zucchelli, Annamaria Nemes, Zoltan Jakus, Kitti Ajtay, Eva Kemecsei, Gabor Kiszler, Nikolett Hegedus, Krisztian Szigeti, Ivan Foldes, Kristof Arvai, Janos Kosa, Kraszimir Kolev, Erzsebet Komorowicz, Parasuraman Padmanabhan, Pal Maurovich-Horvat, Edit Dosa, Gyorgy Varady, Miklos Polos, Istvan Hartyanszky, Sian E. Harding, Bela Merkely, Domokos Mathe, Gabor Szabo, Tamas Radovits, Gabor Foldes
Summary: This study demonstrates that the combination of human induced pluripotent stem cell-derived endothelial cells (hPSC-EC) and biomatrices has the potential to repair ischemic tissues by reconstructing vascular structures and functions. In vitro and in vivo experiments showed that these cells can develop beneficial properties such as antifibrotic, vasoactive and anti-inflammatory effects during recellularization.
Article
Cell & Tissue Engineering
Alexander Bluemke, Erica Ijeoma, Jessica Simon, Rachel Wellington, Medania Purwaningrum, Sergei Doulatov, Elizabeth Leber, Marta Scatena, Cecilia M. Giachelli
Summary: This study compared the ability of two different iPSC lines (PBMC-derived and fibroblast-derived) and two differentiation protocols (embryoid body-based and monolayer-based) to differentiate into osteoclasts. The results showed that embryoid body-based differentiation was successful in generating osteoclasts, while monolayer-based differentiation was not. There was no difference in osteoclast differentiation between the two iPSC lines.
STEM CELL RESEARCH & THERAPY
(2023)
Review
Clinical Neurology
Daisuke Ito, Satoru Morimoto, Shinichi Takahashi, Kensuke Okada, Jin Nakahara, Hideyuki Okano
Summary: Significant progress has been made in drug screening for amyotrophic lateral sclerosis using patient-derived induced pluripotent stem cells, leading to the identification of candidate drugs and the advancement of clinical trials. This stem cell-based approach shows safety and positive effects, reducing costs and time compared to animal testing.
Article
Biotechnology & Applied Microbiology
Ryota Tamura, Hiroyuki Miyoshi, Kent Imaizumi, Masahiro Yo, Yoshitaka Kase, Tsukika Sato, Mizuto Sato, Yukina Morimoto, Oltea Sampetrean, Jun Kohyama, Munehisa Shinozaki, Atsushi Miyawaki, Kazunari Yoshida, Hideyuki Saya, Hideyuki Okano, Masahiro Toda
Summary: This study demonstrates the potential benefit of gene-edited induced pluripotent stem cells (iPSCs) for the treatment of invasive glioma stem cells (GSCs). Neural stem cells (NSCs) derived from CRISPR/Cas9-edited iPSCs expressing a suicide gene showed enhanced tumor-trophic migratory capacity and significant in vivo antitumor effects. The study also identified key signaling pathways and gene expressions associated with the efficacy of therapeutic iPSC-NSCs.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Article
Cell Biology
Satoru Morimoto, Koichi Saeki, Masaru Takeshita, Kunio Hirano, Mariko Shirakawa, Yumiko Yamada, Shiho Nakamura, Fumiko Ozawa, Hideyuki Okano
Summary: The study developed an intranasal vaccine for COVID-19 by using a Sendai virus vector loaded with the receptor binding domain of SARS-CoV-2 as an antigen. The vaccine induced a strong immune response, with increased levels of S-RBD-specific antibodies in serum and bronchoalveolar lavage fluid. The booster dose further enhanced the production of neutralizing antibodies, indicating the effectiveness of the vaccine.
Article
Cell Biology
Sho Yoshimatsu, Mayutaka Nakajima, Iki Sonn, Rie Natsume, Kenji Sakimura, Ena Nakatsukasa, Toshikuni Sasaoka, Mari Nakamura, Takashi Serizawa, Tsukika Sato, Erika Sasaki, Hongkui Deng, Hideyuki Okano
Summary: This study compared the gene expression profiles of mouse, human, and common marmoset EPSCs using transcriptomic approaches. The marmoset EPSC-like cells displayed a distinct morphology but exhibited a pluripotent state similar to embryonic stem cells. They also showed interspecies chimeric contribution to mouse embryos. Furthermore, the gene expression of the marmoset EPSC-like cells resembled that of human EPSCs. Overall, this study evaluated the efficacy of the method for deriving marmoset EPSCs.
Article
Cell & Tissue Engineering
Yuji Okano, Yoshitaka Kase, Hideyuki Okano
Summary: The emergence of single-cell RNA sequencing has revolutionized the study of cellular diversity. Conventional methods for annotating RNA-seq data have limitations in capturing universal characteristics of a cell type. This study presents a new framework for managing cellular identities based on set theory, allowing for comparisons across datasets while preserving biological semantics.
Article
Biochemistry & Molecular Biology
Tosho Kondo, Ihori Ebinuma, Hirotaka Tanaka, Yukitoshi Nishikawa, Takaki Komiya, Mitsuru Ishikawa, Hideyuki Okano
Summary: In this study, an effective high-throughput screening system was established using induced pluripotent stem cells (iPSCs) to generate motor neurons. The generated motor neurons exhibited characteristics similar to ALS neurons and showed abnormal protein accumulation and hyperexcitability. Treatment with rapamycin and retigabine normalized activity and improved neuronal survival. This study provides a valuable platform for discovering novel ALS therapeutics and personalized medicine approaches.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell & Tissue Engineering
Shota Okura, Honoka Ishii, Ayano Suzuki, Chika Saegusa, Ko Fujiki, Kenshi Sugano, Noriomi Suzuki, Tsubasa Saeki, Saeko Matsuzaki, Hiroyuki Ozawa, Masato Fujioka, Makoto Hosoya, Hideyuki Okano
Summary: We have established two human-induced pluripotent stem cell (iPSC) lines from individuals without auditory disorders and confirmed normal hearing through extensive audiometry tests. These iPSC lines express pluripotency genes and are capable of differentiating into the three germ layers. They will be used as controls for pathological analysis and drug screening for ear disorders.
STEM CELL RESEARCH
(2023)
Article
Cell & Tissue Engineering
Ryotaro Imai, Ryota Tamura, Masahiro Yo, Mizuto Sato, Mariko Fukumura, Kento Takahara, Yoshitaka Kase, Hideyuki Okano, Masahiro Toda
Summary: Despite limited treatment options for traumatic brain injury (TBI), neural stem/progenitor cells (NS/PCs) derived from induced pluripotent stem cells (iPSCs) expressing yCD-UPRT showed promising results in functional recovery. The transplanted iPSC-derived NS/PCs displayed stable expression of the therapeutic gene and concentrated around the damaged cortex, leading to improved behavioral performance and prevention of secondary brain injury in a TBI mouse model. In addition, selective elimination of undifferentiated NS/PCs using 5-fluorocytosine (5-FC) demonstrated the safety of this regenerative medicine approach.
Article
Biology
Miho Isoda, Tsukasa Sanosaka, Ryo Tomooka, Yo Mabuchi, Munehisa Shinozaki, Tomoko Andoh-Noda, Satoe Banno, Noriko Mizota, Ryo Yamaguchi, Hideyuki Okano, Jun Kohyama
Summary: A single-cell approach identified cellular properties of neural and mesenchymal origins in human induced pluripotent stem cells, shedding light on the causes of undesired grafts after transplantation.
COMMUNICATIONS BIOLOGY
(2023)
Article
Immunology
Emi Qian, Masahiro Uemura, Hiroya Kobayashi, Shiho Nakamura, Fumiko Ozawa, Sho Yoshimatsu, Mitsuru Ishikawa, Osamu Onodera, Satoru Morimoto, Hideyuki Okano
Summary: CARASIL is a inherited small vessel disease caused by mutations in the HTRA1 gene. Heterozygous mutations in HTRA1 have been found to cause clinical features of the disease. Researchers have successfully established a hiPSC line from a patient with heterozygous HTRA1-related CSVD, which has normal morphology and karyotype. The hiPSCs expressed pluripotency-related markers and had the ability to differentiate into all three germ layers in vitro, providing a valuable tool for studying the cellular mechanisms of HTRA1 mutation.
INFLAMMATION AND REGENERATION
(2023)
Article
Biochemistry & Molecular Biology
Hitomi Ono-Minagi, Tsutomu Nohno, Takashi Serizawa, Yu Usami, Takayoshi Sakai, Hideyuki Okano, Hideyo Ohuchi
Summary: This study investigates the embryonic origin of salivary and lacrimal glands using transgenic mice and demonstrates that these glands contain cells derived from all three germ layers. Specifically, a subset of cells derived from neural crest differentiate into epithelial cells and express high levels of stem cell markers. These cells play an important role in the regenerative response following tissue damage.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Clinical Neurology
Hiroya Kobayashi, Koji Ueda, Satoru Morimoto, Mitsuru Ishikawa, Nicolas Leventoux, Ryogen Sasaki, Yoshifumi Hirokawa, Yasumasa Kokubo, Hideyuki Okano
Summary: The proteins in extracellular vesicles derived from astrocytes in patients with Kii ALS/PDC exhibit distinctive patterns compared to healthy individuals, with increased proteins related to proteostasis and decreased proteins related to anti-inflammation.
NEUROLOGICAL SCIENCES
(2023)
Review
Medicine, General & Internal
Takahiro Shibata, Syoichi Tashiro, Masaya Nakamura, Hideyuki Okano, Narihito Nagoshi
Summary: Cell transplantation therapy using human-induced pluripotent stem cell-derived neural stem/progenitor cells has shown efficacy in treating the subacute phase of spinal cord injury. However, there is a lack of focus on treatment in the chronic phase, which poses challenges in overcoming factors specific to chronic SCI. Combining different therapies and addressing issues in severe injury models and functional recovery are important for regenerative therapy in chronic SCI.
MEDICINA-LITHUANIA
(2023)
Article
Geriatrics & Gerontology
Shohei Komaki, Masatoshi Nagata, Eri Arai, Ryo Otomo, Kanako Ono, Yukiko Abe, Hideki Ohmomo, So Umekage, Natsuko Shinozaki, Tsuyoshi Hachiya, Yoichi Sutoh, Yayoi Otsuka-Yamasaki, Yasumichi Arai, Nobuyoshi Hirose, Akio Yoneyama, Hideyuki Okano, Makoto Sasaki, Yae Kanai, Atsushi Shimizu
Summary: This study explored the epigenetic signatures of exceptional healthy longevity and found that it depends not only on maintaining young epigenetic states but also on advanced states of specific epigenetic regions.
LANCET HEALTHY LONGEVITY
(2023)
Review
Biochemistry & Molecular Biology
Hideyuki Okano, Satoru Morimoto, Chris Kato, Jin Nakahara, Shinichi Takahashi
Summary: In recent years, clinical trials using iPSC technology for disease modeling and drug discovery have shown promising results, particularly for the treatment of ALS. ROPi has been identified as a potential ALS treatment and has undergone clinical trials and reverse translational research, demonstrating its ability to delay disease progression and prolong survival in ALS patients.
JOURNAL OF NEUROCHEMISTRY
(2023)
