Article
Biochemistry & Molecular Biology
Wangyuan Yao, Muhammad Fakhar-e-Alam Kulyar, Yanmei Ding, Haitao Du, Jiajia Hong, Kyein San Loon, Shah Nawaz, Jiakui Li
Summary: The interaction between miR-140 and HDAC4 plays a crucial role in bone disorders such as tibial dyschondroplasia. Low miR-140 expression is associated with poor pathological characteristics and low chondrocyte survival. HDAC4 inhibits MEF2C and Runx2 and interacts with Col-II, Col-X, and COMP, leading to uncontrolled chondrocyte proliferation, differentiation, and dysplastic growth. Targeting the miR-140 and HDAC4 feedback loop may provide a promising therapeutic approach for chondrocyte-related bone disorders.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Fernanda Toscano-Marquez, Yair Romero, Marco Espina-Ordonez, Jose Cisneros
Summary: Idiopathic pulmonary fibrosis (IPF) is a chronic and fatal disease characterized by progressive and irreversible lung scarring associated with fibroblast activation. This study investigates the role of HDAC3, a histone deacetylase, in chromatin remodeling and gene expression in IPF-derived fibroblasts. The results show that HDAC3 is decreased in the nucleus of IPF fibroblasts and its inhibition leads to increased expression of profibrotic genes. The study also suggests that matrix stiffness, mimicked by hydrogels, promotes the loss of nuclear HDAC3 and worsens the fibrotic microenvironment. These findings indicate that HDAC3 could be a potential target for therapeutic interventions in IPF.
Article
Cell Biology
Henry F. Duncan, Yoshifumi Kobayashi, Yukako Yamauchi, Angela Quispe-Salcedo, Zhi Chao Feng, Jia Huang, Nicola C. Partridge, Teruyo Nakatani, Jeanine D'Armiento, Emi Shimizu
Summary: Matrix-metalloproteinase-13 (MMP13) plays an important role in tooth development and mineralization processes. Knockout of MMP13 in mice resulted in altered dentin phenotype, reduced dentin volume, and decreased calcium deposition. The study also revealed an interaction between MMP13 and the Wnt-signaling pathway. These findings highlight the critical role of MMP13 in tooth development and mineralization.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
K. Gomathi, N. Akshaya, N. Srinaath, M. Rohini, N. Selvamurugan
Summary: Bone remodeling is a complex event crucial for maintaining bone homeostasis, with histone acetylation playing a key role in the process. Studies have shown that histone acetyltransferases are major promoters of acetylation, and noncoding RNAs are also involved in regulating acetylation. Understanding these regulatory mechanisms may offer new insights for epigenetic therapies of bone diseases.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Review
Biochemistry & Molecular Biology
Svetlana Demyanenko, Svetlana Sharifulina
Summary: HDAC and HAT play crucial roles in regulating cell functions through acetylating/deacetylating histones and non-histone proteins, impacting cell survival, death, and other processes. The effects of stroke on non-histone protein acetylation/deacetylation in brain cells are still poorly understood, but HDAC inhibitors have shown promise in protecting the brain from ischemic damage. The roles of different HDAC isoforms in brain cell survival and death after stroke remain controversial.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Jinhui Zhao, Wei Zhang, Jaime A. Teixeira da Silva, Xuncheng Liu, Jun Duan
Summary: HDA704 enhances drought and salt tolerance in rice by regulating stomatal closure and density, as well as repressing the expression of DST and ABIL2 through histone deacetylation modification.
Article
Multidisciplinary Sciences
Rodolfo Soria-Castro, Yatsiri G. Meneses-Preza, Gloria M. Rodriguez-Lopez, Alfredo Ibarra-Sanchez, Claudia Gonzalez-Espinosa, Sonia M. Perez-Tapia, Fabian Flores-Borja, Sergio Estrada-Parra, Alma D. Chavez-Blanco, Rommel Chacon-Salinas
Summary: The study found that valproic acid (VPA) inhibits the activation of mast cells (MC) during Listeria monocytogenes (L.m) infection, reducing degranulation and cytokine release. VPA also reduces intracellular calcium increase and phosphorylation of key signaling molecules in MC. These findings suggest that VPA can modulate the functional activity of immune cells involved in controlling L.m infection.
SCIENTIFIC REPORTS
(2022)
Article
Medicine, Research & Experimental
Ann-Kathrin Rahm, Teresa Wieder, Dominik Gramlich, Mara Elena Muller, Maximilian N. Wunsch, Fadwa A. El Tahry, Tanja Heimberger, Tanja Weis, Patrick Most, Hugo A. Katus, Dierk Thomas, Patrick Lugenbiel
Summary: The study revealed that the expression of atrial KCNN2 and KCNN3 is reduced in AF complicated by HF, with a suppression of HDAC2 expression. High atrial rates trigger epigenetic remodeling mechanisms, and knock-down of HDAC2 decreases KCNN3 expression.
Article
Medicine, Research & Experimental
Pascal Syren, Ann-Kathrin Rahm, Patrick A. Schweizer, Claus Bruehl, Hugo A. Katus, Norbert Frey, Dierk Thomas, Patrick Lugenbiel
Summary: The study suggests that the suppression of histone deacetylase 2 (HDAC2) is associated with ventricular electrical remodeling, ion channel expression, and the pathophysiological process of early heart failure.
Review
Immunology
Jie Xu, Linxin Yu, Feng Liu, Longbiao Wan, Zhenhua Deng
Summary: The complicated connections and cross talk between the skeletal system and the immune system are developing into the field of Osteoimmunology. Cytokines, such as TNF, IL, IFN, and chemokines, play a critical role in bone remodeling, particularly in the pathogenesis and development of osteoporosis. This review summarizes the effects of cytokines on osteoblasts and osteoclasts, offering a reference for the role of immunology in osteoporosis.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Cell Biology
Yujian Dai, Jialei Sun, Na He, Liyou An, Chunhui Hou, Fuliang Du
Summary: The regulatory function of the nucleosome remodeling and deacetylase (NuRD) complex in maintaining the pluripotency of naive mouse embryonic stem cells (mES) cultured in a protein kinase C inhibitor (PKCi) system is explored in this study. It is found that PKCi suppresses the expression of NuRD complex subunits and knockdown of MBD3, a subunit of NuRD complex, increases the pluripotency of PKCi-derived mES.
CELL DEATH DISCOVERY
(2022)
Article
Biochemistry & Molecular Biology
Guadalupe J. Jasso, Alok Jaiswal, Mukund Varma, Tyler Laszewski, Angelo Grauel, Abdifatah Omar, Nilsa Silva, Glenn Dranoff, Jeffrey A. Porter, Keith Mansfield, Viviana Cremasco, Aviv Regev, Ramnik J. Xavier, Daniel B. Graham
Summary: By performing single-cell RNA sequencing on stromal cells derived from the colon, this study identified different classes of fibroblasts with distinct gene signatures regulated by chronic inflammation, including IL-11-producing inflammatory fibroblasts. The study also revealed the transcriptional program associated with trans-differentiation of mucosa-associated fibroblasts and defined a functional gene signature linked to matrix deposition and remodeling in the inflamed colon. The critical role of metalloprotease Adamdec1 at the interface between tissue remodeling and healing during colitis was demonstrated, highlighting its requirement for colon epithelial integrity.
Article
Cell Biology
Tuo Zhang, Meina He, Lihua Zhao, Shaogang Qin, Zijian Zhu, Xinhua Du, Bo Zhou, Yi Yang, Xinfeng Liu, Guoliang Xia, Tengxiang Chen, Yuanxi Wang, Hua Zhang, Chao Wang
Summary: The primordial follicle pool established during perinatal development is a non-renewable resource that determines female fertility in mammals. HDAC6 functions as a key negative regulator of mTOR in dormant primordial follicles, playing a crucial role in balancing the maintenance and activation of primordial follicles.
CELL DEATH & DISEASE
(2021)
Article
Biochemistry & Molecular Biology
Qinglang Mei, Xiaohan Xu, Danling Gao, Yuting Xu, Jinbo Yang
Summary: This study demonstrates that LAQ upregulates the Notch signaling pathway in solid tumor cell lines at the molecular level. It also shows that the combination of LAQ and a Notch signaling pathway inhibitor has a synergistic effect in inhibiting solid tumor cell line proliferation in vitro and significantly inhibits tumor cell growth in vivo using an allograft tumor model. Inhibition of the Notch signaling pathway provides a valuable strategy for enhancing solid tumor sensitivity to LAQ.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, General & Internal
Qi Ning, Ye-Hua Gan, Rui-Rui Shi, Juan-Hong Meng
Summary: The study found that HDAC4 has an inhibitory effect on IL-1 beta-induced chondrocyte extracellular matrix degradation, which is partially regulated through the WNT3A/beta-catenin signaling pathway.
CHINESE MEDICAL JOURNAL
(2021)
Article
Genetics & Heredity
Ying Zhang, Ling Ling, Arya Ajay D. O. Ajayakumar, Yating Michelle Eio, Andre J. van Wijnen, Victor Nurcombe, Simon M. Cool
Summary: This study reveals a positive correlation between FGFR2 expression and osteogenic potential in human adult bone marrow-derived MSCs. Knockdown of FGFR2 leads to downregulation of pro-osteogenic genes and upregulation of pro-adipogenic genes, suppressing osteogenesis. Furthermore, FGFR2 knockdown results in upregulation of EZH2, an enzyme that regulates MSC lineage commitment and suppresses osteogenesis under osteogenic induction.
Editorial Material
Orthopedics
Andre J. van Wijnen, Eric A. Lewallen
JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME
(2022)
Review
Cell Biology
Rachel L. Cohn, Nathan S. Gasek, George A. Kuchel, Ming Xu
Summary: Senescent cells play a significant role in aging and disease, and targeting them could slow down the aging process. The commonly used marker p16 has led to important discoveries, but new markers and investigating heterogeneity are crucial. Advanced technologies like single-cell RNA-seq can help uncover new markers and the urgent need to investigate senescence heterogeneity.
TRENDS IN CELL BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Coralee E. Tye, Prachi N. Ghule, Jonathan A. R. Gordon, Fleur S. Kabala, Natalie A. Page, Michelle M. Falcone, Kirsten M. Tracy, Andre J. van Wijnen, Janet L. Stein, Jane B. Lian, Gary S. Stein
Summary: This study reveals a long non-coding RNA (MIR181A1HG) that is highly expressed in multipotent mesenchymal stromal cells (MSCs) and down-regulated during osteogenic differentiation. Knockdown of MIR181A1HG impedes cell cycle progression in MSCs and enhances their differentiation into osteo-chondroprogenitors. This study suggests that MIR181A1HG may serve as a regulator of new bone formation in skeletal disorders.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Insug O-Sullivan, Arivarasu Natarajan Anbazhagan, Gurjit Singh, Kaige Ma, Stefan J. Green, Megha Singhal, Jun Wang, Anoop Kumar, Pradeep K. Dudeja, Terry G. Unterman, Gina Votta-Velis, Benjamin Bruce, Andre J. van Wijnen, Hee-Jeong Im
Summary: The study found that probiotic therapy with Lactobacillus acidophilus can rapidly alleviate inflammatory knee joint pain and prevent further progression of osteoarthritis. This treatment also reduces the levels of cartilage-degrading enzymes, pain markers, and inflammatory factors. Additionally, the probiotic treatment significantly alters the fecal microbiota.
Article
Genetics & Heredity
Parisa Dashti, Jeroen van de Peppel, Roman Thaler, Christopher R. Paradise, Gary S. Stein, Martin A. Montecino, Johannes P. T. M. van Leeuwen, Bram J. van der Eerden, Amel Dudakovic, Andre J. van Wijnen
Summary: This study found that Smyd2 inhibits proliferation and mineral deposition by osteoblasts, and operates parallel to the suppressive effects of Ezh2 and H3K27 trimethylation on osteoblast differentiation.
Review
Biochemistry & Molecular Biology
Jinsha Koroth, Erick O. Buko, Rebecca Abbott, Casey P. Johnson, Brenda M. Ogle, Laura S. Stone, Arin M. Ellingson, Elizabeth W. Bradley
Summary: This narrative review discusses the hallmarks of intervertebral disc degeneration, presents evidence of macrophage involvement during disc degeneration, and explores emerging research on the molecular pathways regulating macrophage functions during intervertebral disc degeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Endocrinology & Metabolism
Megan M. Weivoda, Elizabeth W. Bradley
Summary: Bone remodeling is crucial for maintaining bone quality by removing and replacing damaged and old bone. This process requires tight coordination between bone resorption and bone formation. Besides osteoclasts and osteoblasts, macrophages and other myeloid lineage cells have been found to support bone remodeling. This article discusses the origin and functions of macrophages in the bone microenvironment, including tissue resident macrophages, osteomacs, and newly identified osteomorphs resulting from osteoclast fission. The role of macrophages in inflammatory bone resorption is also touched upon.
JOURNAL OF BONE AND MINERAL RESEARCH
(2023)
Editorial Material
Orthopedics
Andre J. van Wijnen, Eric A. Lewallen
JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME
(2023)
Article
Endocrinology & Metabolism
Jessica Murphy, Bjorn T. Tam, James L. Kirkland, Tamara Tchkonia, Nino Giorgadze, Tamar Pirtskhalava, Michael A. Tsoukas, Jose A. Morais, Sylvia Santosa
Summary: This study aimed to examine the effects of obesity onset on markers of senescence in subcutaneous adipose tissue (SAT) before and after weight loss. The results showed that individuals with childhood-onset obesity had higher levels of DNA damage and senescence markers in SAT compared to those with adult-onset obesity. Weight loss reduced DNA damage in SAT, but did not affect senescence markers. Overall, weight loss improved the aging state associated with obesity.
Review
Biochemistry & Molecular Biology
Praneet Dara, Zeba Farooqui, Fackson Mwale, Chungyoul Choe, Andre J. van Wijnen, Hee-Jeong Im
Summary: Chronic pain conditions caused by arthritis have devastating effects on both individuals and society. The main objective of pharmacological treatments is to reduce pain, and several types of drugs have been considered. Naltrexone, an oral-activated opioid antagonist, has been shown to have beneficial effects on chronic pain conditions such as fibromyalgia and multiple sclerosis.
Article
Orthopedics
Hayman Lui, Cedryck Vaquette, Janet M. Denbeigh, Randy Bindra, Andre J. van Wijnen, Sanjeev Kakar
Summary: This study aims to assess the use of growth factors (BMP2 and GDF5) for bone and ligament regeneration in a multiphasic scaffold in a rabbit knee model. The addition of BMP2 and GDF5 in the BLB scaffold resulted in heterotopic bone formation and ligament failure.
JOURNAL OF WRIST SURGERY
(2023)
Article
Biochemistry & Molecular Biology
Ravi Maisuria, Andrew Norton, Cynthia Shao, Elizabeth W. Bradley, Kim Mansky
Summary: This study aims to determine the role of MEF2C in osteoclast differentiation and analyze the skeletal phenotype of Mef2c-cKO mice. The results suggest that MEF2C is critical for osteoclastogenesis and its dysregulation leads to a sex-specific osteopenic phenotype.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Endocrinology & Metabolism
Stephane Blouin, Farzaneh Khani, Phaedra Messmer, Paul Roschger, Markus A. Hartmann, Andre J. van Wijnen, Roman Thaler, Barbara M. Misof
Summary: Vitamin C is crucial for bone health and low levels of serum Vitamin C increase the risk of skeletal fractures. This study examined the effects of Vitamin C on femoral structure, bone formation, and bone mineralization density distribution in mice. The results showed that Vitamin C depletion significantly reduced cortical thickness and bone volume, as well as diminished the amount of newly formed bone tissue. Additionally, Vitamin C supplementation led to higher calcium concentrations and suggested a longer bone tissue age. Female mice were found to be more sensitive to Vitamin C deficiency. These findings highlight the key role of Vitamin C in bone formation rate and its potential in preventing bone-degenerative diseases, especially in females.
JOURNAL OF BONE AND MINERAL RESEARCH
(2023)
Meeting Abstract
Endocrinology & Metabolism
Gabriel Alvares Borges, Angelo Guilatco, Sonya Royzenblat, Iyabode Ajayi, Claire Edwards, Ming Xu, Tamar Tchkonia, James Kirkland, Taxiarchis Kourelis, Matthew Drake, Megan Weivoda
JOURNAL OF BONE AND MINERAL RESEARCH
(2022)