Article
Chemistry, Analytical
Maho Kaminaga, Tadashi Ishida, Toru Omata
Summary: A trapezoid-shaped microvalve was proposed in this study to effectively close deep microchannels for handling large particles. The valve was fabricated using a double-inclined lithography process, and operated by the expansion of a pneumatic balloon.
Article
Chemistry, Analytical
Yang Zhou, Zhibin Yu, Man Wu, Yuwei Lan, Chunping Jia, Jianlong Zhao
Summary: Single-cell analysis and sorting is an important technique in scientific research and industry. This study developed a microfluidic chip that integrates single-cell droplet generation, incubation, and sorting functions using a pneumatic micro-valve. Numerical simulations were used to optimize the design of the valve. Experimental results demonstrated that the integrated chip improved the purity of target droplets and reduced cell loss and labor. This design has various applications such as single-cell sequencing and antibody screening.
Article
Biochemical Research Methods
Md Ehtashamul Haque, Alvaro J. J. Conde, William N. N. MacPherson, Stephen R. R. Knight, Richard M. M. Carter, Maiwenn Kersaudy-Kerhoas
Summary: A finger-actuated blood lysate preparation device based on acoustofluidic micromixer is developed for effective red blood cell lysis, which has potential applications in various blood-based diagnostic tests. The device features innovative design and user-friendly operation, offering opportunities for the development of low-cost microfluidic-based diagnostics.
Article
Chemistry, Analytical
Chang Chen, Panpan Li, Tianruo Guo, Siyuan Chen, Dong Xu, Huaying Chen
Summary: This study developed a microfluidic device that allows dynamic variations of concentration in a channel and concentration distribution in multiple channels by adjusting the flow resistance using programmable pneumatic microvalves. The device offers superior application potentials in analytical chemistry, drug screening, and cell biology research.
Article
Multidisciplinary Sciences
Ratul Paul, Yuwen Zhao, Declan Coster, Xiaochen Qin, Khayrul Islam, Yue Wu, Yaling Liu
Summary: Microfluidic devices have limitations in terms of low resolution, inferior feature fidelity, and poor repeatability. This study proposes a strategic approach called image-guided in-situ maskless lithography (IGIs-ML) to address these challenges. By using dynamic image analysis and flow control, IGIs-ML provides superior repeatability and fidelity for densely packed features across a large area. This approach enables the fabrication of a wide variety of microfluidic devices and resolves critical limitations in rapid prototyping.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Analytical
Adam Shebindu, Himali Somaweera, Zachary Estlack, Jungtae Kim, Jungkyu Kim
Summary: This study introduces a programmable microfluidic platform (PMP) combined with isotachophoresis (ITP) for fully automated optimization of multiple analytes. By utilizing lifting-gate microvalve technology, a two-dimensional microvalve array was designed and fabricated to seamlessly integrate with an ITP chip for automated buffer selection and repetitive ITP procedures. The optimal buffer combination of 40mM borate and 100mM Tris-HCl successfully preconcentrated all analytes during a single ITP run.
Review
Materials Science, Multidisciplinary
Chenchen Zhou, Yuwei Cao, Chenxu Liu, Wanlin Guo
Summary: Microparticles have attracted extensive interest due to their unique shapes, complex structures, and ability to incorporate various functions. Microfluidic lithography provides a new method for preparing high precision and high throughput microparticles. This review discusses the recent progress of microfluidic lithography methods and the obtained microparticle morphologies, as well as the postprocessing techniques and application prospects for microparticles.
Article
Engineering, Electrical & Electronic
Agnes Beate Bussmann, Claudia Patricia Durasiewicz, Sebastian Heinrich Alexander Kibler, Christian Klaus Wald
Summary: This study presents the design, fabrication, and experimental evaluation of three piezoelectric microfluidic devices for implant automation: a diaphragm pump, a normally closed valve, and a normally open valve. All devices are made of titanium, minimizing the risk of artefacts in medical imaging. They have similar form factors and use the same actuation method, demonstrating outstanding fluidic performance.
SENSORS AND ACTUATORS A-PHYSICAL
(2021)
Article
Biochemical Research Methods
Huichao Chai, Yongxiang Feng, Fei Liang, Wenhui Wang
Summary: In this paper, a novel microfluidic device with high-efficiency cell trapping and deterministic release is presented. The device includes capillary valves that can open in a predefined pattern to sequentially trap incoming cells into separate sites. Experiments using MCF-7 cells demonstrated the capability of the device for deterministic single cell trapping and release, showing promise as a useful platform for single cell analysis.
Article
Biochemical Research Methods
Soohyun Kim, Jayeon Song, RaKyeom Kim, Nae Yoon Lee, Min Hwan Kim, Hyun Gyu Park
Summary: A new centrifugal microfluidic system utilizing ferrowax microvalves has achieved fully automated serial dilutions, with excellent accuracy in a wide dynamic range of up to six orders of magnitude. The overall serial dilution process, including diluent addition, mixing, and product transfer steps, is completed very rapidly within 5 minutes.
BIOTECHNOLOGY JOURNAL
(2021)
Article
Chemistry, Analytical
Pouya Sharbati, Abdolali K. Sadaghiani, Ali Kosar
Summary: This study presents a new dielectrophoretic-based microfluidic device that can accurately separate multiple particle/cell types. The device utilizes cylindrical and sidewall electrodes and has three outlets for different particles. Experiment using various types of particles showed that the device achieved a separation efficiency of 95.5% when sidewall electrodes of 200 mu m were used. The device performed best at different voltages for each separation step, and the electric field remained below the cell electroporation threshold.
Article
Multidisciplinary Sciences
Hao Yang, Mingjie Zhu, Tao Chen, Fuzhou Niu, Lining Sun, Liang Cheng
Summary: Cell mechanics is closely related to cellular functions and can serve as an effective biomarker for disease onset and progression. However, current techniques for measuring cell mechanical properties have limited data and biological significance. This study proposes a dielectrophoresis-based solution that combines a microfluidic device and an automatic control scheme to automatically obtain cell mechanical data. Experiments demonstrate the automation capability of this device.
Article
Chemistry, Analytical
Wei Wang, Haleigh M. Grace, Paul A. Flowers
Summary: This study describes a simple microfluidic device that allows spectroelectrochemical measurements on submicroliter samples. By utilizing embedded electrode chips and a solution-cast ionomeric film, measurements on smaller sample volumes can be achieved.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Analytical
Sho Takamori, Hisatoshi Mimura, Toshihisa Osaki, Shoji Takeuchi
Summary: This paper presents a microfluidic device that utilizes a hydrodynamic pairing system and microelectrodes to achieve accurate electrofusion. The device demonstrated accurate pairing and efficient electrofusion of cell-sized liposomes. It would be a valuable tool in controlling biochemical reactions in artificial cells.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Multidisciplinary Sciences
Allison Clancy, Dayi Chen, Joseph Bruns, Jahnavi Nadella, Samuel Stealey, Yanjia Zhang, Aaron Timperman, Silviya P. Zustiak
Summary: In this study, a hydrogel-based microfluidic cell culture platform was developed, which mimics the glioblastoma microenvironment and is used for drug screening assays.
SCIENTIFIC REPORTS
(2022)
Article
Engineering, Electrical & Electronic
Tadashi Ishida, Van Nguyen Quet, Shota Mochizuki, Yusuke Kagawa, Toshio Takayama, Toru Omata
SENSORS AND ACTUATORS A-PHYSICAL
(2015)
Article
Physics, Applied
Minoru Egawa, Tadashi Ishida, Laurent Jalabert, Hiroyuki Fujita
APPLIED PHYSICS LETTERS
(2016)
Article
Chemistry, Analytical
Tadashi Ishida, Takuya Shimamoto, Nobuya Ozaki, Satoshi Takaki, Takahiro Kuchimaru, Sinae Kizaka-Kondoh, Toru Omata
Article
Nanoscience & Nanotechnology
Takaaki Sato, Shinsuke Nabeya, Vivek Menon, Tadashi Ishida, Reo Kometani, Hiroyuki Fujita
Article
Chemistry, Analytical
Maho Kaminaga, Tadashi Ishida, Tetsuya Kadonosono, Shinae Kizaka-Kondoh, Toru Omata
Article
Chemistry, Analytical
Tadashi Ishida, Takuya Shimamoto, Maho Kaminaga, Takahiro Kuchimaru, Shinae Kizaka-Kondoh, Toru Omata
Article
Chemistry, Analytical
Satoru Kuriu, Tetsuya Kadonosono, Shinae Kizaka-Kondoh, Tadashi Ishida
Article
Chemistry, Analytical
Satoru Kuriu, Naoyuki Yamamoto, Tadashi Ishida
Summary: The small intestine is the main site for host immune cells and immune responses are induced through a gut bacteria sampling process. A new microfluidic device was proposed in this study to observe the fluid flow field supporting microparticle settlement on villi in the small intestine.
Article
Engineering, Electrical & Electronic
Gai Yamauchi, Tadashi Ishida
Summary: A hydraulic microactuator with a coarse/fine drive-switching mechanism was developed to transport cells precisely near sensors in microfluidic devices. The microactuator achieved both coarse approach and fine adjustment of cell position on a thin film toward the microchannel wall by switching drive modes. The displacement ratio achieved using the microactuator was 13.9, with the ability to transport cyanobacteria and adjust their position near the microchannel wall with precision.
SENSORS AND ACTUATORS A-PHYSICAL
(2021)
Article
Chemistry, Analytical
Yasunori Tokuoka, Keiichi Kondo, Noboru Nakaigawa, Tadashi Ishida
Summary: Tissue assays have improved our understanding of cancers by examining the three-dimensional structures and cellular diversity, although certain challenges exist. Microfluidic technology has enabled the development of a device capable of forming concentration gradients in tissues for analysis, demonstrating comparability between appearance-based and content-based measurements.
Article
Chemistry, Analytical
Maho Kaminaga, Tadashi Ishida, Toru Omata
Summary: A trapezoid-shaped microvalve was proposed in this study to effectively close deep microchannels for handling large particles. The valve was fabricated using a double-inclined lithography process, and operated by the expansion of a pneumatic balloon.
Article
Chemistry, Analytical
Akihiko Sugihara, Tadashi Ishida
Summary: This study developed a microfluidic liquid cell with a super-thin electron-transparent membrane (ETM) for high-resolution visualization of nanoscaled biological samples in liquid. The use of this super-thin ETM effectively suppressed electron beam scattering and enabled the observation of sub-microscopic structures.
Article
Biochemical Research Methods
Satoru Kuriu, Naoyuki Yamamoto, Tadashi Ishida
Summary: This study presents a microfluidic device to observe the flow around the villi generated by the dynamic deformation of small intestinal tissue. Using this device, the researchers observed the dynamic flow of the villi and the presence of microbeads adhesion to the villi.
Proceedings Paper
Engineering, Multidisciplinary
Tadashi Ishida
2017 INTERNATIONAL SYMPOSIUM ON MICRO-NANOMECHATRONICS AND HUMAN SCIENCE (MHS)
(2017)