Article
Genetics & Heredity
Xin Liu, Qiu Sun, Qi Wang, Chuansheng Hu, Xuecheng Chen, Hua Li, Daniel M. Czajkowsky, Zhifeng Shao
Summary: Recent study compared the genomic structures of mouse hepatocytes grown in 2D and 3D cultures, and found significant differences in higher-order genomic interactions and up-regulated gene expression associated with physiological hepatocyte functions in the 3D-cultured cells. These results indicate that genomic structure plays a critical role in transcriptional regulation.
GENOMICS PROTEOMICS & BIOINFORMATICS
(2022)
Article
Pharmacology & Pharmacy
Yipeng Xu, Gabriela Pachnikova, Dorothea Przybilla, Reinhold Schafer, Yingying Cui, Dan Zhou, Zihao Chen, An Zhao, Ulrich Keilholz
Summary: This study investigates the inhibitory effect of JQ1 combined with docetaxel on prostate cancer cells. It demonstrates that JQ1 is an effective drug for promoting cell inhibition after docetaxel treatment, and the 3D spheroid culture system accurately simulates the response of prostate cancer cells to drugs.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Materials Science, Composites
Zhiyong Ma, Qifan Wang, Wenjia Xie, Wenjie Ye, Linna Zhong, Jile Huge, Ying Wang
Summary: In this study, a PCL/PLGA/HA scaffold with good pore structure was prepared by 3D printing method. The composite scaffold showed good mechanical and cell test performance, indicating potential applications in the field of bone tissue engineering.
POLYMER COMPOSITES
(2021)
Article
Chemistry, Multidisciplinary
Pengrong Lv, Yuxin You, Junyu Li, Yang Zhang, Dirk J. Broer, Jiawen Chen, Guofu Zhou, Wei Zhao, Danqing Liu
Summary: The paper presents a method based on molecular self-assembling of liquid crystal polymers to directly translate 2D molecular director profiles into 3D topographies without multi-step lithographic processes. By activating LCPs through an electric field, surface deformation can occur to induce topographies governed by the director profile. The approach opens new opportunities for optical and electrical applications.
Article
Polymer Science
Mustafa Ersin Pekdemir, Ecem Oner, Mediha Kok, Ibrahim Nazem Qader
Summary: The shape memory behavior and characterizations of PCL blended with PVC and PMMA were studied in this research. It was found that blending PVC and PMMA with PCL affected the melting temperature and mass loss temperature of the blended polymers. Additionally, the lack of solubility between PCL and PMMA prevented shape recovery, while the PCL:PVC blend showed excellent strain recovery in the shape memory recovering test.
IRANIAN POLYMER JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Malgorzata Nattich-Rak, Marta Sadowska, Maja Motyczynska, Zbigniew Adamczyk
Summary: The adsorption of HSA molecules on negatively charged polystyrene microparticles was studied using various methods, revealing that the isoelectric point of the particles is 5 and the diameter is similar to that of SARS-CoV-2 virus particles.
Review
Biochemistry & Molecular Biology
Elisabetta Colombo, Maria Grazia Cattaneo
Summary: 2D cell cultures have been the standard in various applications, but they cannot replicate the complex and dynamic interactions of the tumor microenvironment (TME). Multicellular 3D models are important for studying the spatial dimension, cellular heterogeneity, and molecular networks of the TME.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Reproductive Biology
Ana Sofia Pais, Sandra Reis, Mafalda Laranjo, Francisco Caramelo, Fatima Silva, Maria Filomena Botelho, Teresa Almeida-Santos
Summary: The study compared conventional (2D) culture with an alginate matrix three-dimensional (3D) model for ovarian tissue culture. The results showed that 3D culture did not have an advantage over 2D culture, as it was more time consuming, challenging to perform, and had worse reproducibility. The 3D culture did show lower cytotoxicity at 24h, but tissue injury was greater with higher interstitial edema and tissue necrosis compared to 2D culture after 72h.
JOURNAL OF OVARIAN RESEARCH
(2021)
Article
Materials Science, Ceramics
Lorenz Hageluken, Pradeep Vallachira Warriam Sasikumar, Ho-Yun Lee, David Di Stadio, Yashoda Chandorkar, Markus Rottmar, Katharina Maniura-Weber, Gurdial Blugan, Juergen Brugger
Summary: In this study, a fast and pressureless process for fabricating SiCN is presented, which exhibits excellent properties in terms of adjustable electrical conductivity, improved flexural strength, and demonstrated cytocompatibility. Furthermore, this processing route is successfully applied to a new multiscale microshaping method combining two-photon polymerization and casting advantages.
JOURNAL OF THE EUROPEAN CERAMIC SOCIETY
(2022)
Article
Biotechnology & Applied Microbiology
Sorel E. De Leon, Lana Cleuren, Zay Yar Oo, Paul R. Stoddart, Sally L. McArthur
Summary: Three-dimensional cell cultures are being used as tools for biologically modeling the human body, requiring sensitive characterization techniques such as impedance spectroscopy. The nature and distribution of cells within the 3D culture, as well as the architecture of the electrodes, are key variables affecting the impedance signal at different frequencies.
BIOENGINEERING-BASEL
(2021)
Article
Biochemistry & Molecular Biology
Simona Braccini, Chiara Tacchini, Federica Chiellini, Dario Puppi
Summary: This article provides an overview of the latest research on in vitro tumor models, with a focus on three-dimensional OC cell culture using hydrogel-based scaffolds. These models can better replicate the tumor microenvironment observed in vivo, making them valuable tools for cancer research and drug screening.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Amanda Pritzlaff, Guillaume Ferre, Emma Mulry, Ling Lin, Niloofar Gopal Pour, Daniel A. Savin, Michael E. Harris, Matthew T. Eddy
Summary: PEGylation is a promising method to improve protein stability, but the underlying mechanism is not well understood. This study used a structural and biophysical approach to investigate the effects of PEGylation on the carbohydrate recognition domain of human galectin-3. The results revealed the potential role of charged residues in PEG localization and provided insights for the rational design of conjugates.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Plant Sciences
Cassandra L. Roberge, Rebecca T. Miceli, Lillian R. Murphy, David M. Kingsley, Richard A. Gross, David T. Corr
Summary: Sophorolipids, derived from nonpathogenic yeasts, have potential efficacy in anticancer applications. They can be synthesized cost-effectively, making them promising alternatives to traditional chemotherapeutics. This study screened two sophorolipids and a clinically-used chemotherapeutic on breast cancer models in various dimensions. The findings demonstrate the importance of 3D tumor models in assessing drug response and suggest that one of the sophorolipids could be comparable in toxicity to the chemotherapeutic control.
JOURNAL OF NATURAL PRODUCTS
(2023)
Article
Biochemistry & Molecular Biology
Garrett Winkelmaier, Kosar Jabbari, Lung-Chang Chien, Peter Grabham, Bahram Parvin, Janice Pluth
Summary: During space travel, astronauts face the challenge of adapting to microgravity and stressful living conditions. The impact of microgravity on organ development is not well understood, and this study used mouse mammary epithelial cells to investigate its effects. The results suggest that microgravity can cause abnormal changes in mammary epithelial cells and increase the risk of cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Polymer Science
Yanmei Wang, Juan Zhang, Mingyang Li, Mingju Lei, Yanen Wang, Qinghua Wei
Summary: This study focuses on the 3D printing performance and shape memory properties of thermoplastic polyurethane and polycaprolactone blends. The results show that 3D printing does not affect the thermal properties, but an increasing TPU content in the blends leads to a decrease in tensile strength.
JOURNAL OF POLYMER RESEARCH
(2022)
Article
Polymer Science
Lisa-Cathrin Leitner, Rika Schneider, Thomas Steiner, Martina H. Stenzel, Ruth Freitag, Andreas Greiner
Summary: This study proposes a concept of microbial remediation for the contamination of wastewater by microplastic particles (MPs). Degradable block copolymers are synthesized to adhere to the surfaces of MPs and attract degrading microbes for accelerated biofilm formation. The properties of the block copolymers, including thermal analysis, colloidal properties, adhesion behavior on MP surfaces, and their potential for supporting microbial growth, are investigated.
MACROMOLECULAR CHEMISTRY AND PHYSICS
(2023)
Article
Polymer Science
Russul Mamdooh Al-Nakashli, Cheng Cao, Radhika Raveendran, Hongxu Lu, Martina H. Stenzel
Summary: Polymeric micelles with different drug loading contents were studied to investigate their cellular uptake, penetration, and effects on biological activities. The results showed that micelles with high drug loading content exhibited higher cellular uptake and toxicity.
MACROMOLECULAR CHEMISTRY AND PHYSICS
(2023)
Article
Biochemistry & Molecular Biology
Mingxia Lu, Sen Wang, Yee Yee Khine, Yanchen Hong, Jiayu Zheng, Hongxu Lu, Martina H. Stenzel
Summary: Ruthenium complexes have potential as alternative anticancer drugs, but their non-specific biodistribution and weak lethality to cancer cells limit their medical use. Drug delivery systems can integrate multiple drugs to enhance chemotherapeutic efficacy and potentially achieve a synergistic effect.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Chemistry, Organic
Feyzi Sinan Tokali, Halil Senol, Tugba Gencoglu Katmerlikaya, Aydan Dag, Kivilcim Sendil
Summary: In this study, 24 new compounds were synthesized and characterized, and compounds 6k and 7f were found to have high anticancer activity, being more selective and potent than the standard drug Doxorubicin.
JOURNAL OF HETEROCYCLIC CHEMISTRY
(2023)
Article
Engineering, Biomedical
Yiling Liu, Stephanie Nemec, Chantal Kopecky, Martina H. Stenzel, Kristopher A. Kilian
Summary: Nanoparticle drug formulations have advantages in cancer therapy, but the assessment of new drugs is limited by preclinical models. This study demonstrates a new approach to test nanomedicine using a microtumor array formed through hydrogel micropatterning. It allows simultaneous evaluation of drug uptake and efficacy in different cancer cell fractions with high reproducibility. The findings suggest that nanoparticle drug formulations are well suited for targeting the stem cell-like population in cancer therapy due to their relationship with drug resistance, recurrence, and metastasis.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Nanoscience & Nanotechnology
Busra Cetin Ersen, Beyza Goncu, Aydan Dag, Gokcen Birlik Demirel
Summary: In this study, targeted and multimodal therapeutic hybrid nanocarriers were fabricated for breast cancer treatments. The carrier consists of synthesized gold nanorods coated with doxorubicin conjugated glycopeptide polymer. A photosensitizer molecule was loaded into the particles for photodynamic therapy, and antisense oligonucleotides were integrated to prevent apoptotic resistance. The biocompatibility and therapeutic efficacy of these nanoparticles were evaluated on human normal skin fibroblast and breast cancer cell lines.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Review
Chemistry, Multidisciplinary
Guocheng Fang, Yu-Cheng Chen, Hongxu Lu, Dayong Jin
Summary: Multicellular spheroids and organoids are promising models in personalized medicine and drug screening, replicating the structural and functional characteristics of human organs. Microfluidic technology and micro-nano fabrication meet the high requirements of engineering approaches in spheroids and organoids research. This review discusses how spheroids- and organoids-on-a-chip technology facilitates their establishment, expansion, and application through spatial-temporal control, mechanical cues modeling, high-throughput analysis, co-culture, multi-tissue interactions, biosensing, and bioimaging integration. The potential opportunities and challenges in developing spheroids- and organoids-on-a-chip technology are also highlighted.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Chemistry, Physical
Lin Zhang, Cheng Cao, Szun S. Tay, Chaohao Chen, Alexander Macmillan, Shihui Wen, Dayong Jin, Joshua McCarroll, Martina H. Stenzel
Summary: Polymer-coated inorganic nanoparticles have unique magnetic, optical, and thermal properties that make them promising drug delivery systems. However, the biological behavior of these nanoparticles is affected by various factors such as size, shape, and protein corona. This study focuses on the effect of drug loading amount on the biological activity of inorganic nanoparticles and highlights the importance of optimizing drug loading amounts in order to achieve optimal drug delivery.
CHEMISTRY OF MATERIALS
(2023)
Article
Materials Science, Multidisciplinary
Ilona Wagner, Simon Spiegel, Julian Brueckel, Matthias Schwotzer, Alexander Welle, Martina H. H. Stenzel, Stefan Braese, Salma Begum, Manuel Tsotsalas
Summary: Surface engineering of MOF NPs using dynamic modification strategies enables the tailoring of PEG-grafted MOF NPs with improved colloidal stability and high dispersity, while maintaining their morphology and lattice crystallinity. The PEG-grafted MOF NPs show reduced adsorption of HSA protein compared to uncoated ones, indicating the potential for reducing nonspecific interactions and introducing targeting moieties for specific interactions. This approach expands the applications of colloidal MOF NPs in biologically relevant fields.
MACROMOLECULAR MATERIALS AND ENGINEERING
(2023)
Article
Polymer Science
Jordan T. Lovegrove, Ben A. Humphreys, Chin Ken Wong, Liliana de Campo, Anna Wang, Christopher J. Garvey, Martina H. Stenzel
Summary: This article investigates a synthetic pathway to produce biologically compatible, crosslinked, multicompartment worms (MCWs) in aqueous solution by incorporating thermoresponsive poly-N-isopropylacrylamide (PNIPAM) to direct assembly above the lower critical solution temperature (LCST). The self-assembly process and mechanism of MCWs starting from primary micelles were observed and controlled by adjusting the temperature.
Article
Medicine, Research & Experimental
Russul Al-Nakashli, Radhika Raveendran, Yee Yee Khine, Cheng Cao, Sophie McKay, Hongxu Lu, Martina H. Stenzel
Summary: This study found that drug loading has an impact on cellular uptake of nanoparticles, which has been overlooked in previous studies focusing on properties such as shape and size. With increasing drug loading content, there was greater dehydration of the polymer shell, leading to increased protein adsorption and particle aggregation. The nanoparticle with the highest drug loading content showed reduced cellular uptake and toxicity in various cell lines. It was concluded that an intermediate drug loading content allows for sufficient cellular uptake and toxic drug concentration.
MOLECULAR PHARMACEUTICS
(2023)
Article
Polymer Science
Jordan Thomas Lovegrove, Radhika Raveendran, Patrick Spicer, Stephan Foerster, Christopher J. Garvey, Martina H. Stenzel
Summary: Margination refers to the movement of particles towards the endothelial wall within blood vessels. This study focused on 2D platelet particles as a potential drug delivery system and quantified their margination propensity. The results showed that 2D platelet particles exhibited increased margination compared to spherical particles, with higher margination observed at higher shear rates.
Article
Biochemistry & Molecular Biology
Zifei Han, Zihao Li, Radhika Raveendran, Shegufta Farazi, Cheng Cao, Robert Chapman, Martina H. Stenzel
Summary: Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) selectively induces apoptosis in cancer cells, but its clinical development is hindered by its short circulation half-life. Researchers have developed core-cross-linked micelles that successfully induce apoptosis in colon cancer cells, offering a potential alternative to TRAIL.
Article
Multidisciplinary Sciences
Chin Ken Wong, Rebecca Y. Lai, Martina H. Stenzel
Summary: Polymersomes, as polymeric analogues of liposomes, have unique physical and chemical properties. However, their translation into clinical or industrial applications has been limited. This study presents a continuous flow methodology for the production of near-monodisperse polymersomes at scale, allowing for downstream polymersome manipulation.
NATURE COMMUNICATIONS
(2023)
Article
Materials Science, Biomaterials
Izabela Stupka, Artur P. Biela, Bernard Piette, Agnieszka Kowalczyk, Karolina Majsterkiewicz, Kinga Borzecka-Solarz, Antonina Naskalska, Jonathan G. Heddle
Summary: Artificial protein cages, such as TRAP-cages, have potential applications in vaccines and drug delivery. TRAP-cages have the ability to control the disassembly conditions by modifying the interface between their building blocks. By using TRAP rings with different numbers of monomers, it is possible to predict the formation of other cages.
JOURNAL OF MATERIALS CHEMISTRY B
(2024)
Article
Materials Science, Biomaterials
Guo Zhang, Yu Wang, Hua Qiu, Lei Lu
Summary: This study presents a one-pot synthesis method for flower-like AMPs@EAMP particles by combining antimicrobial peptides with ellagic acid, offering enlarged surface area, excellent biocompatibility, and broad-spectrum antibacterial activity. In vivo studies indicate their potential for tissue repair and immune barrier reconstruction.
JOURNAL OF MATERIALS CHEMISTRY B
(2024)
Article
Materials Science, Biomaterials
Jiamei Zhang, Lingshuang Wang, Cheng Xu, Yingui Cao, Shengsheng Liu, Rui L. Reis, Subhas C. Kundu, Xiao Yang, Bo Xiao, Lian Duan
Summary: Pluronic F127 modified silk fibroin film with different types of antibacterial agents could accelerate wound recovery by promoting fibroblast adhesion, eradicating bacteria, and facilitating angiogenesis and re-epithelialization.
JOURNAL OF MATERIALS CHEMISTRY B
(2024)
Article
Materials Science, Biomaterials
Yinsheng Liu, Mingyue Wang, Yinfei Hui, Lei Sun, Yanrui Hao, Henlong Ren, Hao Guo, Wu Yang
Summary: In this study, a rare-earth hybrid luminescent material was developed for the detection of a biomarker for anthrax. The material showed excellent selectivity and high sensitivity, allowing for the determination of the biomarker in saliva and urine. Additionally, a convenient point-of-care testing method using fluorescent test paper and a smartphone was established for the initial diagnosis of anthrax.
JOURNAL OF MATERIALS CHEMISTRY B
(2024)
Review
Materials Science, Biomaterials
Wenshuai Yang, Jingsi Chen, Ziqian Zhao, Meng Wu, Lu Gong, Yimei Sun, Charley Huang, Bin Yan, Hongbo Zeng
Summary: Injectable hydrogels with shear-thinning and/or in situ formation properties offer distinct advantages in bioengineering applications, as they can be directly delivered to target sites, possess self-healing abilities, and simplify the implantation process.
JOURNAL OF MATERIALS CHEMISTRY B
(2024)