Article
Engineering, Biomedical
Xueping Wang, Xingquan Xu, Yan Zhang, Xueying An, Xue Zhang, Guoqiang Chen, Qing Jiang, Jun Yang
Summary: The use of cadherin-functionalized microparticles can create a bioartificial stem cell niche that promotes chondrogenesis and cartilage regeneration. These microparticles regulate the response of MSCs and inhibit hypertrophy of chondrocytes, leading to improved regeneration of cartilage and subchondral bone.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Materials Science, Biomaterials
Linxue Zhang, Zhuo Wan, Zuoying Yuan, Jun Yang, Yunfan Zhang, Qing Cai, Jianyong Huang, Yuming Zhao
Summary: In this study, researchers successfully fabricated a microbead-integrated cellular aggregate with multifunctional activities in angiogenesis and osteogenesis. They believe that this innovative approach could be used as a module for bottom-up regenerative medicine and provides a promising method for vascularized bone regeneration.
JOURNAL OF MATERIALS CHEMISTRY B
(2022)
Article
Engineering, Biomedical
Ayana Nakano, Isao Hirata, Binh Vinh Pham, Ajay Shakya, Kotaro Tanimoto, Koichi Kato
Summary: A KL5 decapeptide design was used to tether fusion proteins onto polymer surfaces, with adsorption experiments showing efficiency dependent on protein partners, most effective when fused with acidic proteins like EGF. This method resulted in larger numbers of living human mesenchymal stem cells on specific surfaces, indicating KL5's usefulness as a peptide motif for tethering a specific class of protein partners.
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
(2021)
Article
Cell Biology
Andres Stucky, Li Gao, Shengwen Calvin Li, Lingli Tu, Jun Luo, Xi Huang, Xuelian Chen, Xiaoqing Li, Tiffany H. Park, Jin Cai, Mustafa H. Kabeer, Ashley S. Plant, Lan Sun, Xi Zhang, Jiang F. Zhong
Summary: Using single-cell sequencing, this study discovered a carcinogenic pathway in undifferentiated mesenchymal stem cells (MSCs) of differentiated resistance subclones. The expression of YAP1, OCT4, and CDH6 was upregulated in the subclones and YAP1 inhibition promoted differentiation and suppressed their expression. Clinical data confirmed the co-expression of YAP1, OCT4, and CDH6 in various solid tumors.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Ula V. Jurkunas, Jia Yin, Lynette K. Johns, Sanming Li, Helene Negre, Kit L. Shaw, Lassana Samarakoon, Allison R. Ayala, Ahmad Kheirkhah, Kishore Katikireddy, Alex Gauthier, Stephan Ong Tone, Aaron R. Kaufman, Stacey Ellender, Diego E. Hernandez E. Rodriguez, Heather Daley, Reza Dana, Myriam Armant, Jerome Ritz
Summary: In this study, a novel xenobiotic-free, serum-free, antibiotic-free, two-step process was developed to treat unilateral limbal stem cell (LSC) deficiency. Cultivated autologous limbal epithelial cells (CALEC) were generated from limbal biopsies and expanded onto human amniotic membrane with rigorous quality control. The clinical trial showed preliminary feasibility and safety of CALEC transplants in patients with unilateral LSC deficiency, leading to a second phase of recruitment to collect longer term safety and efficacy data.
Article
Biochemistry & Molecular Biology
Maura Cimino, Paula Parreira, Victoria Leiro, Aureliana Sousa, Raquel M. Goncalves, Cristina C. Barrias, M. Cristina L. Martins
Summary: Efforts have been made to optimize hMSC culture protocols by mimicking the cell physiological microenvironment. The use of pKL surfaces can improve hMSC adhesion and proliferation through selective binding of heparin and fibronectin.
Review
Biochemistry & Molecular Biology
Jessica Doernen, Thomas Dittmar
Summary: Regenerative medicine focuses on investigating therapeutic agents to promote regeneration after injury or in diseases, with MSCs and EVs being of interest for their roles in tissue regeneration. Clinical studies on MSCs have shown conflicting efficacies, and cell fusion may also play a role in regeneration alongside immune responses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Anatomy & Morphology
A. Thirumal Raj, Supriya Kheur, Ramesh Bhonde, Archana A. Gupta, Shankargouda Patil
Summary: This study assessed the effect of human MSC derived conditioned media on human cancer cell lines, identifying pro-carcinogenic and anti-carcinogenic molecules and effector pathways associated with different MSC types. The use of MSC-CM with pro-carcinogenic effect should be restricted in cancer patients regardless of the application nature.
Article
Biochemistry & Molecular Biology
Anuka Sharma, Harmandeep Kaur, Renaissa De, Radhika Srinivasan, Arnab Pal, Shalmoli Bhattacharyya
Summary: The study revealed that cancer stem cells play a crucial role in regulating chemotherapy resistance in cervical cancer cells. Knockdown of E-cadherin induces EMT in cancer cells, imparting stem-cell like characteristics, drug resistance, and invasiveness. This research provides a novel CSC model for cervical cancer cells, which could aid in developing anti-cancer therapies.
BIOCHEMISTRY AND CELL BIOLOGY
(2021)
Review
Cell Biology
Yuan Pan, Yiman Tang, Hang Gu, Wenshu Ge
Summary: This review summarizes the effects and mechanisms of ubiquitin modification on osteogenic differentiation and highlights the latest advances in drug application in bone tissue engineering. Thorough understanding of ubiquitin modification may provide promising therapeutic targets for stem cell-based bone tissue engineering.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Oksana Y. Dudaryeva, Aurelia Bucciarelli, Giovanni Bovone, Florian Huwyler, Shibashish Jaydev, Nicolas Broguiere, Marwa al-Bayati, Marco Lutolf, Mark W. Tibbitt
Summary: The biophysical properties of the cellular microenvironment, such as stiffness and geometry, have been shown to influence cell function, with recent findings implicating 3D confinement as a significant regulator of cell behavior. This study demonstrates that the extent of 3D confinement affects cell viability and proliferation, leading to changes in nuclear morphology and YES-associated protein (YAP) localization. The effects of confinement on cell behavior scale with the degree of 3D confinement, highlighting the importance of 3D confinement as a regulator of cell function.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Agriculture, Dairy & Animal Science
Adelaida Hernaiz, Paula Cobeta, Belen Marin, Francisco Jose Vazquez, Juan Jose Badiola, Pilar Zaragoza, Rosa Bolea, Inmaculada Martin-Burriel
Summary: The study demonstrates for the first time how a three-dimensional culture system improves the susceptibility of ovine mesenchymal stem cells to prion infection, mimicking the toxic effects seen in prion diseases. This model shows potential as an in vitro system for studying prion diseases in a microenvironment similar to in vivo conditions. In neurodegenerative diseases, including prion diseases, cellular in vitro models are important for studying disease mechanisms and potential therapies.
Review
Medicine, Research & Experimental
Jianghong Huang, Qisong Liu, Jiang Xia, Xi Chen, Jianyi Xiong, Lei Yang, Yujie Liang
Summary: This article comprehensively reviews the homing effect of stem cells in joints and highlights progress towards the targeted modification of MSCs.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Article
Cell & Tissue Engineering
Yumi Ohori-Morita, Kunimichi Niibe, Phoonsuk Limraksasin, Praphawi Nattasit, Xinchao Miao, Masahiro Yamada, Yo Mabuchi, Yumi Matsuzaki, Hiroshi Egusa
Summary: This study optimized the shaking culture method using human BM-MSCs by using a neurosphere culture technique and generated MSC spheroids with high expression of MSC-related genes. The NM-spheroids showed enhanced bone regeneration in vivo.
STEM CELLS TRANSLATIONAL MEDICINE
(2022)
Review
Chemistry, Multidisciplinary
He Yang, Lihua Yao, Yichen Wang, Gaojian Chen, Hong Chen
Summary: Biological cells are complex living machines, and the manipulation of their surfaces using synthetic molecules has proven to be an effective means of introducing innovative functionalities or manipulating cells. This review offers a comprehensive survey of recent advancements in the modification of mammalian cell surfaces through the use of synthetic molecules. It explores a range of strategies, including chemical covalent modifications, physical alterations, and bioorthogonal approaches. The review also addresses the present challenges and potential future opportunities in this rapidly expanding field.
Article
Chemistry, Multidisciplinary
Huayu Wu, Dan Zhong, Zhijun Zhang, Yahui Wu, Yunkun Li, Hongli Mao, Kui Luo, Deling Kong, Qiyong Gong, Zhongwei Gu
Summary: MGBMs involve fabricating materials by learning from natural species, with great potential for biomedical applications. AMBs were constructed inspired by bacterial therapy for cancer treatment, possessing a novel nitric oxide generation strategy.
Article
Biotechnology & Applied Microbiology
Xingyu Hou, Dan Zhong, Yunkun Li, Hongli Mao, Jun Yang, Hu Zhang, Kui Luo, Qiyong Gong, Zhongwei Gu
Summary: A novel dual-responsive multi-pocket nanoparticle was constructed through self-assembly and cross-linking of amphiphilic methoxypolyethylene glycol-lipoic acid (mPEG-LA) conjugates to enhance tumor penetration and antitumor efficacy. The multi-pocket nanoparticles showed prolonged blood circulation, deep penetration in tumor tissues, and rapid drug release in intracellular environment, demonstrating superior antitumor activities with reduced side effects.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Yunkun Li, Dan Zhong, Chuan Zhou, Zhaoxu Tu, Hongli Mao, Jun Yang, Hu Zhang, Kui Luo, Qiyong Gong, Zhongwei Gu
Summary: A hybrid theranostic nanoplatform of less than 50 nm is developed using a template supramolecular strategy for active targeting and deep penetration of primary and metastatic tumors, particularly those with poor vascular structures. This multifunctional nanoplatform shows significant therapeutic effects on both primary tumors and distant metastases in a xenograft tumor model, with real-time monitoring of therapeutic responses enabled by the inherent fluorescence of the quantum dots.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Chemistry, Applied
Jie Zhou, Shengnan Ma, Yuxin Zhang, Yiyan He, Hongli Mao, Jun Yang, Hu Zhang, Kui Luo, Qiyong Gong, Zhongwei Gu
Summary: A study has successfully developed sequentially targeted therapeutic nanocomplexes that can overcome challenges in the tumor microenvironment, achieving nuclear translocation of DNA through receptor-mediated endocytosis and enhancing gene expression while inducing apoptosis in tumor cells through the integration of gene and photodynamic therapy. These bacterium-mimicking STNPs show promising potential as a candidate for tumor treatments.
CARBOHYDRATE POLYMERS
(2021)
Article
Chemistry, Applied
Yuxin He, Yang Li, Yadong Sun, Shijia Zhao, Miao Feng, Guoming Xu, Haofang Zhu, Peihong Ji, Hongli Mao, Yiyan He, Zhongwei Gu
Summary: The double-network polysaccharide-based hydrogel showed excellent mechanical properties, biological activities, and biocompatibility, effectively promoting wound healing. Experimental results demonstrated that the hydrogel accelerated wound healing in a mouse skin defect model through multiple pathways.
CARBOHYDRATE POLYMERS
(2021)
Article
Chemistry, Multidisciplinary
Seiichi Tada, Xueli Ren, Hongli Mao, Yun Heo, Shin-Hye Park, Takashi Isoshima, Liping Zhu, Xiaoyue Zhou, Reiko Ito, Shino Kurata, Megumi Osaki, Eiry Kobatake, Yoshihiro Ito
Summary: Artificial materials are important for medical devices such as artificial organs and matrices for regenerative medicine. In this study, mitogenic and differentiation-inducible materials are devised via the simple coating of polypeptides, which enable increased cell growth and differentiation through long-lasting signal transduction triggered by bound growth factors. This superior growth and differentiation are attributed to not causing receptor internalization and subsequent downregulation.
Article
Chemistry, Multidisciplinary
Yuhang Xing, Xueqin Qing, Hao Xia, Shiqi Hao, Haofang Zhu, Yiyan He, Hongli Mao, Zhongwei Gu
Summary: An injectable hydrogel tissue adhesive containing adipic acid dihydrazide-modified gelatin and oxidized sodium alginate was developed in this study, showing excellent adhesion strength and favorable biocompatibility. The adhesive exhibited a uniform structure, appropriate swelling ratio, good injectability, and excellent hemocompatibility and cytocompatibility, making it a promising candidate for soft-tissue adhesives.
FRONTIERS IN CHEMISTRY
(2021)
Article
Engineering, Biomedical
Yuhang Jiang, Wentao Zhao, Shuangshuang Xu, Jingjing Wei, Fernando Lopez Lasaosa, Yiyan He, Hongli Mao, Rosa Maria Bolea Bailo, Deling Kong, Zhongwei Gu
Summary: Research has shown that mannose-decorated globular lysine dendrimers (MGLDs) can elicit anti-inflammatory activity by targeting and reprogramming macrophages, which may be beneficial in the therapy of injuries and inflammation.
Article
Chemistry, Applied
Hongli Mao, Shijia Zhao, Yuxin He, Miao Feng, Lihuang Wu, Yiyan He, Zhongwei Gu
Summary: This study developed a polysaccharide-based hydrogel dressing with excellent properties, which accelerated wound healing and improved skin regeneration through in situ photocrosslinking.
CARBOHYDRATE POLYMERS
(2022)
Article
Pharmacology & Pharmacy
Xiangming Kong, Miao Feng, Lihuang Wu, Yiyan He, Hongli Mao, Zhongwei Gu
Summary: The 10-year survival rate of patients with pancreatic cancer is still low due to incomplete surgery and lack of effective postoperative treatment. This study developed a biodegradable microdevice for local sustained drug delivery to effectively inhibit postoperative recurrence with minimal systemic toxicity.
Article
Engineering, Biomedical
Haofang Zhu, Guoming Xu, Yiyan He, Hongli Mao, Deling Kong, Kui Luo, Wenbo Tang, Rong Liu, Zhongwei Gu
Summary: In this study, a double-bioinspired hydrogel adhesive with a strong tissue adhesion and enhanced toughness was developed. The adhesive showed fast, strong, and durable adhesion to wet biological tissues. Compared to commercial bio-glues and traditional adhesives, the adhesive exhibited significantly better tissue adhesion both ex vivo and in vivo.
ADVANCED HEALTHCARE MATERIALS
(2022)
Review
Materials Science, Biomaterials
Lihuang Wu, Yiyan He, Hongli Mao, Zhongwei Gu
Summary: This review provides an overview of recent progress in bioactive hydrogels based on polysaccharides and peptides for soft tissue wound management. The relationship between the chemical structures and inherent bioactive functions of polysaccharides and peptides, as well as strategies for achieving multiple wound repair effects, are discussed. The review also introduces innovative and important works in this field and discusses current study limitations, clinical unmet needs, and future directions.
JOURNAL OF MATERIALS CHEMISTRY B
(2022)
Review
Materials Science, Biomaterials
Ke Zhou, Yadong Sun, Jiquan Yang, Hongli Mao, Zhongwei Gu
Summary: Three-dimensional (3D) bioprinting plays a crucial role in tissue engineering. The emerging solution of embedded bioprinting addresses the challenges faced in traditional bioprinting by depositing bioinks into a support bath. This review focuses on the progress of hydrogels used as bioinks and support baths.
JOURNAL OF MATERIALS CHEMISTRY B
(2022)
Review
Engineering, Biomedical
Haofang Zhu, Jiang Tian, Hongli Mao, Zhongwei Gu
Summary: Bioadhesives have advantages over conventional sutures, but existing adhesives often have issues such as cytotoxicity, fragility, or low adhesive strength. Therefore, there is a need to develop a safe and effective tissue adhesive to overcome these problems.
CURRENT OPINION IN BIOMEDICAL ENGINEERING
(2021)
Article
Chemistry, Multidisciplinary
Yahui Wu, Dan Zhong, Yunkun Li, Huayu Wu, Hu Zhang, Hongli Mao, Jun Yang, Kui Luo, Qiyong Gong, Zhongwei Gu
Summary: Combinatorial short interference RNA (siRNA) technology is utilized to silence multiple genes in cancer treatment. A tumor-activatable supramolecular nanoplatform is designed and synthesized to successfully deliver dual-gene targeted combinatorial siRNAs for effective inhibition of tumor cell growth and neovascularization in drug-resistant cancers.
Article
Materials Science, Biomaterials
Izabela Stupka, Artur P. Biela, Bernard Piette, Agnieszka Kowalczyk, Karolina Majsterkiewicz, Kinga Borzecka-Solarz, Antonina Naskalska, Jonathan G. Heddle
Summary: Artificial protein cages, such as TRAP-cages, have potential applications in vaccines and drug delivery. TRAP-cages have the ability to control the disassembly conditions by modifying the interface between their building blocks. By using TRAP rings with different numbers of monomers, it is possible to predict the formation of other cages.
JOURNAL OF MATERIALS CHEMISTRY B
(2024)
Article
Materials Science, Biomaterials
Guo Zhang, Yu Wang, Hua Qiu, Lei Lu
Summary: This study presents a one-pot synthesis method for flower-like AMPs@EAMP particles by combining antimicrobial peptides with ellagic acid, offering enlarged surface area, excellent biocompatibility, and broad-spectrum antibacterial activity. In vivo studies indicate their potential for tissue repair and immune barrier reconstruction.
JOURNAL OF MATERIALS CHEMISTRY B
(2024)
Article
Materials Science, Biomaterials
Jiamei Zhang, Lingshuang Wang, Cheng Xu, Yingui Cao, Shengsheng Liu, Rui L. Reis, Subhas C. Kundu, Xiao Yang, Bo Xiao, Lian Duan
Summary: Pluronic F127 modified silk fibroin film with different types of antibacterial agents could accelerate wound recovery by promoting fibroblast adhesion, eradicating bacteria, and facilitating angiogenesis and re-epithelialization.
JOURNAL OF MATERIALS CHEMISTRY B
(2024)
Article
Materials Science, Biomaterials
Yinsheng Liu, Mingyue Wang, Yinfei Hui, Lei Sun, Yanrui Hao, Henlong Ren, Hao Guo, Wu Yang
Summary: In this study, a rare-earth hybrid luminescent material was developed for the detection of a biomarker for anthrax. The material showed excellent selectivity and high sensitivity, allowing for the determination of the biomarker in saliva and urine. Additionally, a convenient point-of-care testing method using fluorescent test paper and a smartphone was established for the initial diagnosis of anthrax.
JOURNAL OF MATERIALS CHEMISTRY B
(2024)
Review
Materials Science, Biomaterials
Wenshuai Yang, Jingsi Chen, Ziqian Zhao, Meng Wu, Lu Gong, Yimei Sun, Charley Huang, Bin Yan, Hongbo Zeng
Summary: Injectable hydrogels with shear-thinning and/or in situ formation properties offer distinct advantages in bioengineering applications, as they can be directly delivered to target sites, possess self-healing abilities, and simplify the implantation process.
JOURNAL OF MATERIALS CHEMISTRY B
(2024)
