Journal
JOURNAL OF MATERIALS CHEMISTRY B
Volume 4, Issue 15, Pages 2598-2605Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5tb02697k
Keywords
-
Categories
Funding
- Centre National de la Recherche Scientifique (CNRS)
- Lille1 University
- Nord Pas-de-Calais region
- Institut Universitaire de France (IUF)
- European Union through FP7-PEOPLE-IRSES [269009]
- Institut Francais in Romania
Ask authors/readers for more resources
Particle-based photodynamic therapy (PPDT) holds great promise in theranostic applications. Herein, we demonstrate that PPDT based on gold nanorods coated with an indocyanine green (ICG)-loaded silica shell allows for the inactivation of the Crohn's disease-associated adherent-invasive Escherichia coli strain LF82 (E. coli LF82) under pulsed laser light irradiation at 810 nm. Fine-tuning of the plasmonic structures together with maximizing the photosensitizer loading onto the nanostructures allowed optimizing the singlet oxygen generation capability and the PPDT efficiency. Using a nanoparticle concentration low enough to suppress photothermal heating effects, 6 log(10) reduction in E. coli LF82 viability could be achieved using gold nanostructures displaying a plasmonic band at 900 nm. An additional modality of nanoparticle-based photoinactivation of E. coli is partly observed, with 3 log(10) reduction of bacterial viability using Au NRs@SiO2 without ICG, due to the two-photon induced formation of reactive oxygen species. Interaction of the particles with the bacterial surface, responsible for the disruption of the bacterial integrity, together with the generation of moderate quantities of singlet oxygen could account for this behavior.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available