4.6 Article

Chitosan-based core-shell structured particles for in vivo sustainable gene transfection

Journal

JOURNAL OF MATERIALS CHEMISTRY B
Volume 4, Issue 5, Pages 893-901

Publisher

ROYAL SOC CHEMISTRY
DOI: 10.1039/c5tb02074c

Keywords

-

Funding

  1. National Natural Science Foundation of China [81171829, 51103143, 51173175, 51473152, 51573175]
  2. Provincial Natural Science Foundation of Anhui [KJ2011ZD04]
  3. Fundamental Research Funds for Central Universities [WK2060200012, WK3450000001]

Ask authors/readers for more resources

A core-shell structured chitosan (CS)-based gene vector with a sustainable gene transfection effect was designed and successfully prepared in this study. The pEGFP was first combined with the thiolated and N-alkylated chitosan (TACS). Then, hydroxybutyl chitosan grafted with poly(ethylene glycol) (EG-HBC) was coated on the pEGFP-loaded TACS particles. The prepared pEGFP-loaded TACS@EG-HBC particles have a size of about 200 nm and little cytotoxicity. The in vitro and in vivo gene transfection experiments indicate that the pEGFP-loaded TACS@EG-HBC particles possess a better sustainable gene transfection capacity and a high transfection efficiency, which should be attributed to the biodegradation of the CS-based shell, the thiolation and N-alkylation modification on CS cores, and the grafted PEG chains with better biocompatibility. The in vivo gene expression of the loaded pEGFP can persist up to 60 days. This novel gene vector has a theoretical and practical significance for gene therapy with sustained transfection effect.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.6
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

Article Chemistry, Multidisciplinary

Chitosan modified by γ-ray-induced grafting of poly(tributyl-(4-vinylbenzyl)phosphonium) as a biosafe and high-efficiency gene carrier

Kun Zeng, Fu-xing Lin, Juan Xie, Mo-zhen Wang, Jie-lin Rong, Yu Zhao, Ye-zi You, Anila Asif, Xue-wu Ge

NEW JOURNAL OF CHEMISTRY (2017)

Article Chemistry, Physical

Formation mechanism of 3D macroporous graphene aerogel in alcohol-water media under gamma-ray radiation

Weikang Wang, Yihu Wu, Zhiwen Jiang, Mozhen Wang, Qichao Wu, Xiao Zhou, Xuewu Ge

APPLIED SURFACE SCIENCE (2018)

Article Chemistry, Multidisciplinary

Self-assembly of graphene oxide nanosheets in t-butanol/water medium under gamma-ray radiation

Weikang Wang, Yihu Wu, Zhiwen Jiang, Mozhen Wang, Qichao Wu, Xiao Zhou, Xuewu Ge

CHINESE CHEMICAL LETTERS (2018)

Article Materials Science, Multidisciplinary

Cagelike porous sulfonated polystyrene@polyaniline composite microspheres for high-performance supercapacitor

Hanhong Huang, Jianhua Lu, Mozhen Wang, Xuewu Ge

JOURNAL OF MATERIALS SCIENCE (2018)

Article Physics, Atomic, Molecular & Chemical

Gamma Ray Radiation Effect on Bi2WO6 Photocatalyst

Qiang Zhang, Zhi-wen Jiang, Mo-zhen Wang, Xue-wu Ge

CHINESE JOURNAL OF CHEMICAL PHYSICS (2018)

Article Chemistry, Multidisciplinary

Space-Confined Seeded Growth of Black Silver Nanostructures for Solar Steam Generation

Jinxing Chen, Ji Feng, Zhiwei Li, Panpan Xu, Xiaojing Wang, Wenwen Yin, Mozhen Wang, Xuewu Ge, Yadong Yin

NANO LETTERS (2019)

Article Chemistry, Physical

Fabrication of inverse-opal lysozyme-imprinted polydopamine/polypyrrole microspheres with near-infrared-light-controlled release property

Wenxiu Yang, Kun Zeng, Jiaxin Liu, Lechen Chen, Mozhen Wang, Shengchi Zhuo, Xuewu Ge

JOURNAL OF COLLOID AND INTERFACE SCIENCE (2019)

Article Chemistry, Multidisciplinary

Preparation and adsorption property of novel inverse-opal hierarchical porous N-doped carbon microspheres

Wenxiu Yang, Guoqing Xu, Jingjing Shu, Mozhen Wang, Xuewu Ge

Summary: In this work, novel N-doped carbon microspheres with high surface area and adsorption capacity were successfully obtained through a series of preparation steps. This method provides a new approach for the preparation of high-performance hierarchical porous carbon materials.

CHINESE CHEMICAL LETTERS (2021)

Article Chemistry, Multidisciplinary

Integrated Evaporator for Efficient Solar-Driven Interfacial Steam Generation

Jinxing Chen, Bo Li, Guoxiang Hu, Rashed Aleisa, Shan Lei, Fan Yang, Dilong Liu, Fenglei Lyu, Mozhen Wang, Xuewu Ge, Fang Qian, Qiao Zhang, Yadong Yin

NANO LETTERS (2020)

Article Energy & Fuels

Preparation and performance of magnetic phase change microcapsules with organic-inorganic double shell

Zhuoni Jiang, Jingjing Shu, Zhiqing Ge, Zhiwen Jiang, Mozhen Wang, Xuewu Ge

Summary: Magnetic microcapsulated phase change materials with high thermal conductivity, rapid magnetic response, and excellent reusability have been developed for directional temperature regulation through magnetic localization. The new material is expected to play an important role in solar energy conversion utilization.

SOLAR ENERGY MATERIALS AND SOLAR CELLS (2022)

Article Chemistry, Physical

Ni-nanoparticle-bound boron nitride nanosheets prepared by a radiation-induced reduction-exfoliation method and their catalytic performance

Zhiwen Jiang, Wengang Zhu, Guoqing Xu, Xiangjianfei Xu, Mozhen Wang, Hongbing Chen, Wei Huang, Xuewu Ge, Mingzhang Lin

JOURNAL OF MATERIALS CHEMISTRY A (2020)

Article Materials Science, Biomaterials

Biodegradable nano-organosilica gene carrier for high-efficiency gene transfection

Kun Zeng, Li Ma, Wenxiu Yang, Shan Lei, Mozhen Wang, Yezi You, Yu Zhao, Xuewu Ge

JOURNAL OF MATERIALS CHEMISTRY B (2020)

Article Polymer Science

Construction of polyporous polymer microspheres with a tailored mesoporous wall

Hanqin Weng, Zhihao Wu, Chi Zhao, Mozhen Wang, Xuewu Ge, Shinichi Yamashita, Jia Tang, Mingzhang Lin

POLYMER CHEMISTRY (2019)

Article Physics, Atomic, Molecular & Chemical

γ-Ray-Radiation-Scissioned Chitosan as a Gene Carrier and Its Improved in vitro Gene Transfection Performance

Fu-xing Lin, Kun Zeng, Wen-xiu Yang, Mo-zhen Wang, Jie-lin Rong, Juan Xie, Yu Zhao, Xue-wu Ge

CHINESE JOURNAL OF CHEMICAL PHYSICS (2017)

Article Materials Science, Biomaterials

An artificial protein cage made from a 12-membered ring

Izabela Stupka, Artur P. Biela, Bernard Piette, Agnieszka Kowalczyk, Karolina Majsterkiewicz, Kinga Borzecka-Solarz, Antonina Naskalska, Jonathan G. Heddle

Summary: Artificial protein cages, such as TRAP-cages, have potential applications in vaccines and drug delivery. TRAP-cages have the ability to control the disassembly conditions by modifying the interface between their building blocks. By using TRAP rings with different numbers of monomers, it is possible to predict the formation of other cages.

JOURNAL OF MATERIALS CHEMISTRY B (2024)

Article Materials Science, Biomaterials

Facile one-pot synthesis of flower-like ellagic acid microparticles incorporating anti-microbial peptides for enhanced wound healing

Guo Zhang, Yu Wang, Hua Qiu, Lei Lu

Summary: This study presents a one-pot synthesis method for flower-like AMPs@EAMP particles by combining antimicrobial peptides with ellagic acid, offering enlarged surface area, excellent biocompatibility, and broad-spectrum antibacterial activity. In vivo studies indicate their potential for tissue repair and immune barrier reconstruction.

JOURNAL OF MATERIALS CHEMISTRY B (2024)

Article Materials Science, Biomaterials

Transparent silk fibroin film-facilitated infected-wound healing through antibacterial, improved fibroblast adhesion and immune modulation

Jiamei Zhang, Lingshuang Wang, Cheng Xu, Yingui Cao, Shengsheng Liu, Rui L. Reis, Subhas C. Kundu, Xiao Yang, Bo Xiao, Lian Duan

Summary: Pluronic F127 modified silk fibroin film with different types of antibacterial agents could accelerate wound recovery by promoting fibroblast adhesion, eradicating bacteria, and facilitating angiogenesis and re-epithelialization.

JOURNAL OF MATERIALS CHEMISTRY B (2024)

Article Materials Science, Biomaterials

Polyarylether-based COFs coordinated by Tb3+ for the fluorescent detection of anthrax-biomarker dipicolinic acid

Yinsheng Liu, Mingyue Wang, Yinfei Hui, Lei Sun, Yanrui Hao, Henlong Ren, Hao Guo, Wu Yang

Summary: In this study, a rare-earth hybrid luminescent material was developed for the detection of a biomarker for anthrax. The material showed excellent selectivity and high sensitivity, allowing for the determination of the biomarker in saliva and urine. Additionally, a convenient point-of-care testing method using fluorescent test paper and a smartphone was established for the initial diagnosis of anthrax.

JOURNAL OF MATERIALS CHEMISTRY B (2024)

Review Materials Science, Biomaterials

Recent advances in fabricating injectable hydrogels via tunable molecular interactions for bio-applications

Wenshuai Yang, Jingsi Chen, Ziqian Zhao, Meng Wu, Lu Gong, Yimei Sun, Charley Huang, Bin Yan, Hongbo Zeng

Summary: Injectable hydrogels with shear-thinning and/or in situ formation properties offer distinct advantages in bioengineering applications, as they can be directly delivered to target sites, possess self-healing abilities, and simplify the implantation process.

JOURNAL OF MATERIALS CHEMISTRY B (2024)