Article
Biotechnology & Applied Microbiology
Hyeongsoon Kim, Minsik Kim, Sam-Nyu Jee, Sunggi Heu, Sangryeol Ryu
Summary: Pectobacterium carotovorum subsp. carotovorum is a pathogen that causes soft rot disease in crops by producing plant cell wall-degrading enzymes (PCWDEs). The emergence of antibiotic-resistant bacteria and the demand for harmless antimicrobial products have driven the search for alternative therapeutic strategies. In this study, a phage cocktail targeting two different phage receptors was developed to control P. carotovorum subsp. carotovorum. The phage cocktail effectively reduced the populations of P. carotovorum subsp. carotovorum and attenuated its virulence, as demonstrated by decreased PCWDE production and decreased swimming motility in phage-resistant mutants. These results suggest that phage cocktails targeting multiple receptors could be an effective approach for controlling P. carotovorum subsp. carotovorum in crops.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Plant Sciences
Fang Liu, Qian Zhao, Zhenhua Jia, Siyuan Zhang, Juan Wang, Shuishan Song, Yantao Jia
Summary: Long-chained quorum-sensing signals have been shown to play important roles in plant defense responses. The short-chained signal 3OC8-HSL can enhance Arabidopsis and Chinese cabbage resistance to different bacteria through the salicylic acid and jasmonic acid pathways.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Food Science & Technology
Jin Cai, Shiqin Wang, Yichen Gao, Qi Wang
Summary: This study investigated the antibacterial activity, chemical compounds and antibacterial mechanism of Polygonum orientale L. essential oil (POEO) against Pectobacterium carotovorum subsp. carotovorum (Pcc). The results showed that POEO exhibited effective inhibitory activity against Pcc, making it a potential alternative to chemical bactericides for controlling Pcc.
Article
Plant Sciences
Pol Vendrell-Mir, Pierre-Francois Perroud, Fabian B. Haas, Rabea Meyberg, Florence Charlot, Stefan A. Rensing, Fabien Nogue, Josep M. Casacuberta
Summary: Next-generation sequencing techniques have been used to identify new plant viruses, increasing our knowledge beyond economically important crops. A study identified a virus named PHPAV1 infecting Physcomitrium patens, which can be vertically transmitted throughout the plant cell cycle.
Article
Plant Sciences
Katarina Landberg, Jan Simura, Karin Ljung, Eva Sundberg, Mattias Thelander
Summary: Auxin plays a crucial role in plant development, affecting the differentiation of stem cells and gametes. While apical stem cells synthesize auxin, their progeny respond to it. Gamete precursors rely on auxin sensing, but the final differentiation process is low in auxin sensitivity.
Article
Agronomy
Weiyao Yan, Xuan Fu, Yuan Gao, Liyin Shi, Qi Liu, Wenchao Yang, Jianguo Feng
Summary: Copper sulfide nanoparticles (CuS NPs) were prepared and evaluated for their antibacterial activity and safety, showing excellent efficacy against Pectobacterium carotovorum subsp. carotovorum (Pcc) and low toxicity to earthworms and cells. CuS NPs could be utilized as a highly effective, lowly toxic, and environmentally friendly antibacterial agent.
PEST MANAGEMENT SCIENCE
(2022)
Article
Multidisciplinary Sciences
Yanhua Ge, Yi Gao, Yuling Jiao, Ying Wang
Summary: This study reveals the regulation of midrib formation in moss by the orthologs of Arabidopsis LATERAL SUPPRESSOR gene, which is a key regulator of axillary meristem initiation in seed plants. The PpLAS/AtLAS genes have conserved functions in promoting cell division in both lineages, highlighting the importance of the conserved regulatory module in different developmental programs.
Article
Microbiology
Ping-Chen Chung, Ruchi Briam James S. Lagitnay, Reymund C. Derilo, Jian-Li Wu, Yutin Chuang, Jia-De Lin, Duen-Yau Chuang
Summary: Carocin S2 is a bacteriocin produced by Pectobacterium carotovorum subsp. carotovorum 3F3 strain with a low molecular weight. Through mutational research, the active sites of Carocin S2 were identified and it was found that the binding between the immunity protein and the bacteriocin was inhibited by an uncharacterized domain.
Article
Agriculture, Multidisciplinary
Chen Chang-long, Yuan Fang, Li Xiao-ying, Ma Rong-cai, Xie Hua
Summary: The study reveals that Chinese cabbage shows a synergistic defense response against Pc infection with jasmonic acid (JA) and ethylene (ET), with JA-mediated signaling being the most significant.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2021)
Article
Microbiology
Natalia Padilla-Galvez, Paola Luengo-Uribe, Sandra Mancilla, Amandine Maurin, Claudia Torres, Pamela Ruiz, Andres France, Ivette Acuna, Homero Urrutia
Summary: This study aimed to select endophytic actinobacteria from native potatoes in southern Chile for antagonistic activity against Pectobacterium carotovorum subsp. carotovorum and Pectobacterium atrosepticum. It was found that Streptomyces sp. TP199 had significant potential as a biological control agent for the prevention of soft rot and blackleg diseases in potato crops.
Article
Microbiology
Huang-Pin Wu, Reymund C. Derilo, Shih-Hao Hsu, Jia-Ming Hu, Duen-Yau Chuang
Summary: This study reports the discovery, isolation, characterization, and functional analysis of Carocin S4, a novel low-molecular-weight bacteriocin from Pectobacterium carotovorum subsp. carotovorum (Pcc). Carocin S4, composed of CaroS4K and CaroS4I, exhibits deoxyribonuclease activity and hydrolyzes both genomic and plasmid DNA. This study reveals another nuclease type of bacteriocin in Pcc, with CaroS4K and CaroS4I functioning as killer and immunity proteins, respectively.
Article
Biochemistry & Molecular Biology
Chung-Pei Chang, Ruchi Briam James Sersenia Lagitnay, Tzu-Rong Li, Wei-Ting Lai, Reymund Calanga Derilo, Duen-Yau Chuang
Summary: This study investigated the role of the catabolite activator protein (CAP) in the regulation of carocin synthesis in Pcc. Knockout of the crp gene resulted in inhibition of genes related to bacteriocin export and affected the production of low-molecular-weight bacteriocins. UV induction influenced the binding preference of CRP to the CAP sites.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agronomy
Xin Zhan, Rui Wang, Manman Zhang, Yuejiao Li, Tao Sun, Jie Chen, Jishun Li, Tong Liu
Summary: The study demonstrated that emodin from Trichoderma asperellum LN004 acts as a quorum sensing inhibitor (QSI) by competitively binding both ExpI and ExpR proteins in Pcc, interfering with its quorum sensing system and reducing the production of virulence factors. The research provides the first molecular mechanism revealing emodin's ability as a QSI to competitively inhibit two QS proteins simultaneously.
PEST MANAGEMENT SCIENCE
(2023)
Article
Agricultural Engineering
Mojgan Nouri, Sareh Baghaee-Ravari, Bahareh Emadzadeh
Summary: The study showed that nano-emulsified savory and thyme oils have antibacterial properties and can be effective in preventing soft rot disease in potato tubers.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Biochemistry & Molecular Biology
Yanjiao Qi, Mingyang Wang, Bo Zhang, Yue Liu, Jiaqin Fan, Zifan Wang, Li Song, Peer Mohamed Abdul, Hong Zhang
Summary: Rheum tanguticum extract (RTMBE) is found to have antibacterial activity against drug-resistant phytopathogenic bacteria by inhibiting key protein expressions and attenuating their virulence.
Article
Plant Sciences
Shaoqun Zhou, Ying K. Zhang, Karl A. Kremling, Yezhang Ding, John S. Bennett, Justin S. Bae, Dean K. Kim, Hayley H. Ackerman, Michael V. Kolomiets, Eric A. Schmelz, Frank C. Schroeder, Edward S. Buckler, Georg Jander
Article
Multidisciplinary Sciences
Leonardo Delgado-Cerrone, Alfonso Alvarez, Eilyn Mena, Ines Ponce de Leon, Marcos Montesano
Article
Plant Sciences
Eilyn Mena, Silvina Stewart, Marcos Montesano, Ines Ponce de Leon
FRONTIERS IN PLANT SCIENCE
(2020)
Article
Microbiology
Guillermo Reboledo, Astrid Agorio, Lucia Vignale, Ramon Alberto Batista-Garcia, Ines Ponce De Leon
Summary: Botrytis cinerea is a necrotrophic pathogen that infects a wide range of plant species, including moss Physcomitrium patens. Genomic analysis showed that B. cinerea uses reactive oxygen species, plant cell wall degradation, toxin production, and effector proteins to evade plant defenses and cause decay. Comparisons with RNAseq data from angiosperm infections suggest shared and specific virulence strategies used by B. cinerea across different host plants.
Article
Biochemistry & Molecular Biology
Guillermo Reboledo, Astri D. Agorio, Lucia Vignale, Ramon Alberto Batista-Garcia, Ines Ponce De Leon
Summary: The study identified molecular mechanisms underlying the defense response of the bryophyte Physcomitrium patens against the plant pathogen Botrytis cinerea, with a total of 3,072 differentially expressed genes significantly affected. The results highlight the existence of evolutionary conserved defense responses to pathogens throughout the green plant lineage, suggesting they were likely present in the common ancestors of land plants. The differential expression of genes acquired from horizontal transfer and moss-specific orphan genes during B. cinerea infection indicates their importance in the moss immune response.
PLANT MOLECULAR BIOLOGY
(2021)
Review
Plant Sciences
Guillermo Reboledo, Astrid Agorio, Ines Ponce De Leon
Summary: This review summarizes the current knowledge on transcription factor families that regulate development and responses to stress in mosses and other bryophytes. The study shows that these families play crucial roles in regulating plant growth and stress responses, and highlights their conserved and distinct biological functions compared with other plants.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Agronomy
Paula Conde-Innamorato, Claudio Garcia, Juan Jose Villamil, Facundo Ibanez, Roberto Zoppolo, Mercedes Arias-Sibillotte, Ines Ponce De Leon, Omar Borsani, Georgina Paula Garcia-Inza
Summary: Evaluating the production response of olive trees to irrigation is crucial, especially in regions with no tradition of olive production and humid climates. Results showed that proper irrigation can increase fruit weight and pulp/pit ratio, but the response of oil content and polyphenols varies among cultivars.
Article
Biotechnology & Applied Microbiology
Eilyn Mena, Silvia Garaycochea, Silvina Stewart, Marcos Montesano, Ines Ponce De Leon
Summary: In this study, the genomic and transcriptomic information of D. caulivora, a fungal pathogen causing stem canker in soybean, was analyzed to gain insights into its molecular basis of pathogenicity. The study revealed a large number of genes encoding secreted pathogenicity-related proteins in D. caulivora, and identified various factors related to host infection strategies, including plant cell wall degradation and modification, compound detoxification, transporter activities, and toxin production.
Article
Plant Sciences
Adam D. Steinbrenner, Evan Saldivar, Nile Hodges, Natalia Guayazan-Palacios, Antonio F. Chaparro, Eric A. Schmelz
Summary: Chewing herbivores activate plant defense responses through a combination of mechanical wounding and elicitation by herbivore-associated molecular patterns. This study investigates the specific signaling and defense responses mediated by HAMP in cowpea plants.
Article
Plant Sciences
Guillermo Reboledo, Astrid Agorio, Lucia Vignale, Alfonso Alvarez, Ines Ponce De Leon
Summary: This study reveals the presence of a pathogen-inducible ERF gene family PpERF24 in moss plants, which is absent in liverworts, ferns, and angiosperms. PpERF24 enhances plant defenses against fungal pathogens by regulating the expression of defense genes involved in transcriptional regulation, phenylpropanoid and jasmonate pathways, oxidative burst, and pathogenesis-related genes. These findings provide novel insights into the defense mechanisms of moss plants.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Katherine M. Murphy, Tyler Dowd, Ahmed Khalil, Si Nian Char, Bing Yang, Benjamin J. Endelman, Patrick M. Shih, Christopher Topp, Eric A. Schmelz, Philipp Zerbe
Summary: In maize, two major groups of specialized metabolites, kauralexins and dolabralexins, are involved in defending against pathogens, herbivores, and other stressors. This study examined the dolabralexin pathway and found new metabolites and characterized their production. Genetic analysis showed that dolabralexin biosynthesis occurs mainly in primary roots and varies across different maize lines. Loss-of-function mutants for the diterpene synthase gene ZmKSL4 demonstrated deficient dolabralexin production and exhibited altered root architecture in response to water deficit. These findings suggest that maize dolabralexins play a role in plant vigor during abiotic stress.
Article
Plant Sciences
Evan V. Saldivar, Yezhang Ding, Elly Poretsky, Skylar Bird, Anna K. Block, Alisa Huffaker, Eric A. Schmelz
Summary: In maize, fungal-elicited immune responses lead to the production of various sesquiterpenoids and diterpenoids as antibiotics. Through metabolic profiling, a chromosome 1 locus associated with five candidate sesquiterpenoids was identified. Enzyme co-expression studies confirmed the production of specific terpenoids by ZmTPS27 and ZmTPS8. In vitro bioassays showed that the terpenoid cubebol, produced by ZmTPS8, exhibits significant antifungal activity.
Article
Multidisciplinary Sciences
Devon Birdseye, Laura A. de Boer, Hua Bai, Peng Zhou, Zhouxin Shen, Eric A. Schmelz, Nathan M. Springer, Steven P. Briggs
Summary: The study revealed that the overexpression of chloroplast ribosomal proteins in hybrid plants is mediated by reduced expression of ethylene biosynthetic enzymes, and this overexpression can quantitatively predict hybrid vigor in adult plants. This study sheds light on the molecular basis of hybrid vigor.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)