Article
Plant Sciences
Dimitri L. L. Fonseka, Samuel G. G. Markell, Marcio L. L. Zaccaron, Malaika K. K. Ebert, Julie S. S. Pasche
Summary: Globally, Ascochyta blight in field pea crops is caused by a complex of specific fungal pathogens, and the use of foliar fungicides is common for disease management. However, understanding the prevalence, aggressiveness, and sensitivity of these pathogens to fungicides is crucial for effective control.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Robert C. Lee, Christina R. Grime, Kane O'Driscoll, Yuphin Khentry, Lina M. Farfan-Caceres, Hediyeh Tahghighi, Lars G. Kamphuis
Summary: This study screened 1,276 Pisum spp. germplasm accessions and identified low disease score pea varieties. These varieties were crossed with Australian field pea varieties to develop recombinant inbred line populations, which were then phenotyped and genotyped. Marker-trait associations were identified, and potential candidate resistance genes were found.
Article
Agronomy
Ana Margarida Sampaio, Diego Rubiales, Maria Carlota Vaz Patto
Summary: The study revealed that two Fusarium oxysporum strains isolated from grass pea are host-specific, infecting only grass pea with low aggressiveness, while grass pea could also be infected by a strain from pea. These results highlight the importance of conducting host-range studies, even on specialist pathogens, to identify potential alternative hosts and improve disease control management.
Review
Microbiology
Emiliano Foresto, Maria Evangelina Carezzano, Walter Giordano, Pablo Bogino
Summary: Chickpea, a crucial legume for many countries, is highly susceptible to Ascochyta blight caused by Ascochyta rabiei fungus. The pathogenesis of the disease and plant defense mechanisms against the pathogen are still poorly understood. Understanding these aspects is essential for crop protection.
Article
Microbiology
Na Liu, Chao Liu, Yajing Song, Xingmin Han, Guwen Zhang, Zhijuan Feng, Bin Wang, Yuanpeng Bu, Jinwen Ou, Yaming Gong
Summary: Ascochyta pisi is identified as a pathogen causing ascochyta blight of pea in China, and its fully annotated genome is reported in this study. Comparative genome analysis reveals the genetic diversity and evolutionary relationship of Ascochyta species. In planta transcriptome analysis provides valuable information for further understanding the interaction between Ascochyta spp. and legumes.
MICROBIOLOGY SPECTRUM
(2023)
Article
Multidisciplinary Sciences
Sara Fondevilla, Nicolas Krezdorn, Diego Rubiales, Bjoern Rotter, Peter Winter
Summary: This study identified genes associated with resistance to Peyronellaea pinodes in pea crops and discovered crucial mechanisms against the pathogen. The study also found expression marker genes that can distinguish resistant from susceptible plants.
SCIENTIFIC REPORTS
(2022)
Article
Microbiology
Emmanuel N. N. Annan, Bernard Nyamesorto, Qing Yan, Kevin McPhee, Li Huang
Summary: Ascochyta blight (AB) is a destructive disease of field peas caused by necrotrophic fungal pathogens. To identify resistant individuals, low-cost and reliable protocols for AB screening are needed. We tested and optimized three protocols to determine the best type of pathogen inoculum, host development stage, and timing of inoculation for detached-leaf assays.
Article
Plant Sciences
Felipe A. Castro-Urrea, Maria P. Urricariet, Katia T. Stefanova, Li Li, Wesley M. Moss, Andrew L. Guzzomi, Olaf Sass, Kadambot H. M. Siddique, Wallace A. Cowling
Summary: The accuracy of predicted breeding values (PBV) for low heritability traits can be increased by utilizing information from correlated traits. A study compared the accuracy of PBV for 10 correlated traits in a genetically diverse field pea population using univariate or multivariate linear mixed model (MLMM) analysis. The MLMM approach improved the accuracy of PBV and showed potential for increasing genetic gains in early generation selection cycles.
Article
Plant Sciences
Sameer Joshi, Babu Ram Pandey, Garry Rosewarne
Summary: Ascochyta blight is a destructive disease in field pea caused by several necrotrophic pathogens. Understanding disease epidemiology and resistance mechanisms is important for breeding resistant varieties. This study analyzed phenotypic, histochemical, and molecular responses in pea plants infected with different pathogens, providing a comprehensive understanding of the infection process and resistance mechanisms.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Agronomy
Getachew Gudero Mengesha, Habtamu Terefe, Abu Jambo Yae, Asinake Abera Arato, Merihun Gimja Betrire, Tamirat Samuel Shago, Zemenu Fentahun Bires, Biniyam Boraysho Borano, Shiferaw Mekonnen Abebe
Summary: This study investigated the control methods for Ascochyta blight in field peas, including the integrated use of host resistance and fungicides. The results showed that fungicide-treated plots had the slowest disease progression, while control plots had the fastest progression. Fungicide applications significantly reduced disease severity and increased yield in field peas. Economically, the combination of Othello-Top and the Burqitu variety was the most profitable management option.
ACTA AGRICULTURAE SCANDINAVICA SECTION B-SOIL AND PLANT SCIENCE
(2022)
Article
Plant Sciences
Frankie Kay Crutcher, Yesuf Assen Mohammed, Chengci Chen, Sherry Turner
Summary: Ascochyta blight is a major threat to chickpea production worldwide. This study found that using resistant cultivars is essential for disease control and maximizing yield, while fungicide applications alone are not enough.
Article
Plant Sciences
R. Bowness, B. D. Gossen, K. F. Chang, C. J. Willenborg, R. L. Conner, S. E. Strelkov
Summary: In the management of Mycosphaerella blight in field pea, double-nozzle configurations can effectively reduce disease severity and increase yield, while droplet size and angle of application have no effect on yield. Water volumes up to 400 L ha(-1) improve fungicide efficacy, but volumes above 400 L ha(-1) can lead to increased disease severity and decreased yield due to fungicide run-off. Factors such as disease pressure, environmental conditions, and cultivar characteristics are important considerations when deciding on effective sprayer techniques for fungicide application.
CANADIAN JOURNAL OF PLANT PATHOLOGY
(2021)
Article
Agronomy
Lucio Valetti, Luis Ignacio Cazon, Clara Crociara, Silvina Pastor
Summary: Ascochyta blight is a major disease affecting chickpeas worldwide, with early detection and rapid identification of the pathogen being crucial for disease management. The PCR-based method described in this study offers a rapid and accurate detection of A. rabiei, aiding in effective disease management practices.
Article
Ecology
Agathe Dutt, Rault Anthony, Didier Andrivon, Stephane Jumel, Gwenola Le Roy, Alain Baranger, Melen Leclerc, Christophe Le May
Summary: The study found that co-infection can alter the life history traits of pathogens and affect their coexistence within hosts. Co-infection results in resources being concentrated on lesion development, leading to shorter latent periods and decreased offspring production. The direction and magnitude of these changes depend on the life history strategies of co-infecting strains, with responses always higher in self-pairs than in mixed infections.
Article
Agronomy
Rabia Faridi, Birger Koopman, Antje Schierholt, Mohamed B. Ali, Stefanie Apel, Wolfgang Link
Summary: This study used genome-wide association study to identify markers associated with Ascochyta blight resistance in winter faba beans, finding one marker on chromosome III co-localizes with a known resistance QTL. These new markers have the potential to improve resistance breeding in winter faba beans and support broader inclusion of the crop in rotations.
Review
Agronomy
Diego Rubiales, Hamid Khazaei
Summary: Faba bean is an important grain legume crop, but is susceptible to various diseases and pests. DNA markers have been developed for some diseases, but not yet for others. The combination of traditional breeding and new technologies is speeding up the development of resistant cultivars.
THEORETICAL AND APPLIED GENETICS
(2022)
Article
Plant Sciences
Martin Weih, Eveline Adam, Giulia Vico, Diego Rubiales
Summary: Intercropping enhances biodiversity and resource use efficiency in agriculture. Breeding for intercropping can improve intercrop performance by maximizing niche complementarity. Crop growth models can assist breeding for intercropping, but should incorporate relevant plant features, be calibrated with field data, and favor simplicity.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Agronomy
Diego Rubiales, Ana Moral, Fernando Flores
Summary: Multi-environment field testing of chickpea accessions in Southern Spain showed that environmental effects have a greater impact on yield compared to genotypic effects and genotype-environment interaction (GEI). Ascochyta blight infection was found to have the most detrimental effect on grain yield. High temperatures during the reproductive stage, particularly with a Tmax >30 degrees C, had negative effects on yield. Genotypic effects were more significant in relation to ascochyta infection when accessions previously selected for resistance or susceptibility were included.
Review
Plant Sciences
Osman Z. Wohor, Nicolas Rispail, Chris O. Ojiewo, Diego Rubiales
Summary: Pea is a widely cultivated grain legume with important roles in food security. However, its production is constrained by various pests and diseases, especially rhizospheric diseases. Understanding the rhizosphere environment is crucial for improving yield stability and crop performance. Utilizing germplasm and genomic resources, combined with scientific collaboration, has contributed to improving resistance against rhizospheric diseases in pea. However, resistance is still limited, and traditional control strategies are unrealistic or unfavorable to the environment.
Article
Plant Sciences
Salvador Osuna-Caballero, Nicolas Rispail, Eleonora Barilli, Diego Rubiales
Summary: This study investigated the responses of 320 Pisum spp. accessions to U. pisi under field and controlled conditions. The researchers identified genotypes with partial resistance and confirmed that the reduced infection of resistant accessions was associated with smaller rust colonies. A late acting hypersensitive response was also identified for the first time in a pea accession. These findings highlight the importance of screening pea collections for complete resistance and the potential for further breeding perspectives.
Article
Biochemistry & Molecular Biology
Nicolas Rispail, Osman Zakaria Wohor, Salvador Osuna-Caballero, Eleonora Barilli, Diego Rubiales
Summary: By studying the genetic diversity and population structure of a core collection of 325 pea accessions, it was found that the Pisum genus can be classified into two species, P. fulvum and P. sativum, and wild alleles were incorporated into the cultivated pea through the intermediate species P. sativum subsp. jomardii and P. sativum subsp. arvense during pea domestication. These findings have important implications for breeding programs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Eleonora Barilli, Diego Rubiales
Summary: In this study, a large germplasm collection of cultivated lentils and its wild relatives were screened for resistance against lentil rust. The results showed the presence of accessions with hypersensitive reaction-based resistance and partial resistance, as well as the potential for developing a standard differential set for rust definition and monitoring.
Review
Agriculture, Multidisciplinary
Maryam Bagheri, Carla S. Santos, Diego Rubiales, Marta W. Vasconcelos
Summary: Drought, caused by climate change, is a significant obstacle for crop yield. It can hinder plant growth, disrupt water relations, and reduce water use efficiency. Pea, a protein-rich grain legume, has several drought resistance mechanisms through morphological, physiological, and biochemical changes. Strategies like screening, breeding, and marker-assisted selection have been used to manage drought tolerance in peas. Biotechnological approaches have also led to the development of drought-tolerant pea cultivars.
ANNALS OF APPLIED BIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Francisco J. Agudo-Jurado, Pierluigi Reveglia, Diego Rubiales, Antonio Evidente, Eleonora Barilli
Summary: Fungal phytotoxins, as secondary metabolites toxic to host plants, play a role in the development of symptoms in legume diseases by targeting host cellular machineries or interfering with host immune responses. This review focuses on the isolation, chemical and biological characterization of fungal phytotoxins produced by necrotrophic fungi involved in legume diseases. The possible role in plant-pathogen interaction and structure-toxicity relationship studies are also discussed. Additionally, multidisciplinary studies on other biological activities of reviewed phytotoxins are described, along with the challenges in identifying new fungal metabolites and their potential applications in future experiments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Food Science & Technology
Elsa Mecha, Mara Lisa Alves, Andreia Bento da Silva, Ana Barbara Pereira, Diego Rubiales, Maria Carlota Vaz Patto, Maria Rosario Bronze
Summary: To ensure food security and environmental sustainability, legumes are being sought as alternative protein sources. This study characterizes the protein content and quality of legumes, including underutilized grain legumes, and highlights their adaptability to European agricultural systems. Through multivariate analysis, intra- and inter-species variability was identified. Grass pea had the highest protein content but not the highest individual amino acid content and in vitro protein digestibility. Lentil was highlighted for its amino acid content and in vitro protein digestibility. This study brings attention to legume species that are not commonly used in Western diets.
Article
Plant Sciences
Rind Balech, Fouad Maalouf, Somanagouda B. Patil, Karthika Rajendran, Lynn Abou Khater, Diego Rubiales, Shiv Kumar
Summary: Assessing the adaptability and stability of herbicide-tolerant lentil accessions in multi-environment trials is crucial for improving breeding programs. This study investigated the adaptability and stability of 42 herbicide-tolerant lentil accessions using five stability parameters in eight different environments. Genotype-Environment interaction was significant for flowering and maturity time, as well as seed yield. Different stability parameters ranked the accessions differently, highlighting the importance of using a combination of parameters for evaluation. GGE biplot analysis showed that certain accessions were specifically adapted to certain environments and should be included in breeding programs to develop herbicide-tolerant cultivars.
Article
Microbiology
Angel M. Villegas-Fernandez, Ahmed A. Amarna, Juan Moral, Diego Rubiales
Summary: This study assessed the feasibility of using intercropping or mixtures of susceptible and resistant cultivars to control faba bean rust. Results showed that intercropping with barley significantly reduced rust severity on faba bean, while other combinations had no effect. The barrier effect of barley was identified as the main mechanism explaining rust suppression. Cultivar mixture experiments also showed that rust severity decreased as the proportion of the resistant cultivar increased.
Article
Microbiology
Pierluigi Reveglia, Francisco J. Agudo-Jurado, Eleonora Barilli, Marco Masi, Antonio Evidente, Diego Rubiales
Summary: Different fungal species of Colletotrichum genus cause anthracnose disease in major crops, resulting in significant economic losses globally. This study utilized the OSMAC approach integrated with targeted and non-targeted metabolomics profiling to investigate the secondary phytotoxic metabolites produced by pathogenic isolates of Colletotrichum truncatum and Colletotrichum trifolii. The phytotoxicity of the fungal crude extracts was also evaluated on their primary hosts and related legumes, correlating with the metabolite profile under different cultural conditions.
Review
Agronomy
Diego Rubiales, Eleonora Barilli, Nicolas Rispail
Summary: This article provides an overview of recent advancements in the genetic and genomic resource development of peas, including the integration of resistance genes and the mapping of genes/QTLs. By combining conventional and modern breeding strategies, genetic gains can be significantly enhanced.
Article
Plant Sciences
Susana Trindade Leitao, Diego Rubiales, Maria Carlota Vaz Patto
Summary: In this study, new sources of rust resistance were identified in the Portuguese germplasm of common bean. Disease severity-associated SNPs and candidate genes were identified, indicating the diverse genetic mechanisms of rust resistance in bean. These findings provide promising genomic targets for developing molecular tools to support precision breeding for rust resistance in beans.
