Journal
FRONTIERS IN MICROBIOLOGY
Volume 7, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fmicb.2016.01597
Keywords
GH1 beta-glucosidase; metagenomics; metabolic profiling; operational taxonomic units; microbial community
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Funding
- National Fund for Basic, Strategic and Frontier Application Research in Agriculture (ICAR-NEBSFARA) [NFBSFARA/AE-2006/2010-11]
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beta-glucosidase is a crucial element of the microbial cellulose multienzyme complex since it is responsible for the regulation of the entire cellulose hydrolysis process. Therefore, the aim of the present work was to explore the diversity and distribution of glycosyl hydrolase family 1 beta-glucosidase genes in three different environmental niches including, Himalayan soil, cow dung and compost by metagenomic approach. Preliminary evaluation through metabolic profiling using BIOLOG based utilization patterns of carbon, nitrogen, phosphorus and sulfur revealed the environment and substrate specific nature of the indigenous microbial population. Furthermore, clonal library selection, screening and sequence analysis revealed that most of the GH1 beta-glucosidase proteins had low identities with the available database. Analysis of the distribution of GH1 beta-glucosidase gene fragments and beta-glucosidase producing microbial community revealed the environment specific nature. The OTUs obtained from Himalayan soil and compost metagenomic libraries were grouped into 19 different genera comprising 6 groups. The cow dung sample displayed the least diversity of GH1 beta-glucosidase sequences, with only 14 genera, distributed among three groups-Bacteroidetes, Firmicutes, and Actinobacteria. The metagenomic study coupled with metabolic profiling of GH1 beta-glucosidase illustrated the existence of intricate relationship between the geochemical environmental factors and inherent microbial community.
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