Article
Biotechnology & Applied Microbiology
Segolene Calvez, Nora Navarro-Gonzalez, Charlene Siekoula-Nguedia, Catherine Fournel, Eric Duchaud
Summary: This study assessed the genetic diversity of Flavobacterium psychrophilum isolates from apparently healthy rainbow trout from several fish farms in the same watershed in France. High diversity was revealed by two different genotyping methods, with the majority of isolates being unrelated to the predominant clonal complex worldwide. Thirteen novel sequence types were discovered, suggesting a diverse subpopulation of F. psychrophilum in rainbow trout.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Environmental Sciences
Raquel Castillo-Contreras, Marta Marin, Jorge Ramon Lopez-Olvera, Teresa Ayats, Xavier Fernandez Aguilar, Santiago Lavin, Gregorio Mentaberre, Marta Cerda-Cuellar
Summary: Campylobacter and Salmonella are the most commonly reported zoonotic agents in Europe. Wild boars in Barcelona have been found to carry both these pathogens. The prevalence of Campylobacter in wild boars is 61%, with some isolates showing antimicrobial resistance and virulence potential. Salmonella, including a serovar associated with human infections, was also detected in a small percentage of wild boars.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Microbiology
Marketa Nykrynova, Vojtech Barton, Karel Sedlar, Matej Bezdicek, Martina Lengerova, Helena Skutkova
Summary: Genotyping methods can be used to distinguish bacterial strains, and the proposed method based on variable genetic segments can help identify transmission routes and sources of highly virulent and multiresistant strains.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Xiaona Wei, Wei Chen, Qianjin Sun, Qian Zhong, Zhuanqian Yan, Qingfeng Zhou, Yongchang Cao, Feng Chen, Xiangbin Zhang
Summary: In this study, it was found that among 487 suspected M. synoviae infection samples in China, 324 samples were positive, with a positive rate of 66.53%. A total of 104 strains were isolated and genotyped using the MLST method, and they belonged to 8 sequence types, with ST-34 being the most prevalent. BURST analysis showed that these 104 isolates were grouped together with 56 other strains isolated from China, indicating their similarity and independence from strains abroad.
Article
Infectious Diseases
Luka Jurinovic, Sanja Duvnjak, Andrea Humski, Biljana Jecmenica, Louie Thomas Taylor, Borka Simpraga, Fani Krstulovic, Tajana Amsel Zelenika, Gordan Kompes
Summary: This study is important for understanding the transmission and antimicrobial resistance of C. lari bacteria in seagulls. By sampling 1753 seagulls and sequencing the DNA of C. lari, the study discovered 31 new sequence types and antimicrobial-resistant mutations. These findings contribute to the prevention and control of gastrointestinal diseases in humans.
Article
Microbiology
Mariana Meneguzzi, Caroline Pissetti, Raquel Rebelatto, Julian Trachsel, Suzana Satomi Kuchiishi, Adrienny Trindade Reis, Roberto Mauricio Carvalho Guedes, Joice Aparecida Leao, Caroline Reichen, Jalusa Deon Kich
Summary: Clinical salmonellosis has been increasing significantly in Brazil, with a majority of strains demonstrating high rates of multi-drug resistance. The identification of a major Choleraesuis clonal group in several Brazilian states suggests closely related strains. This highlights the endemic nature of salmonellosis in key pig-producing regions in Brazil.
Review
Infectious Diseases
Hassanain Al-Talib, Syahirah Samsudin, Ariza Adnan, Chandrika Murugaiah
Summary: This review examines the prevalence and changing patterns of MRSA strains in the Malaysian population from 2002 to the present. The study found that MRSA strains in Malaysia are genetically related and ST239, ST1, and ST22 are the most common sequence types. The majority of MRSA strains in Malaysia exhibit SCCmec types III, IV, and V.
TROPICAL MEDICINE AND INFECTIOUS DISEASE
(2022)
Article
Biotechnology & Applied Microbiology
Ibai Nafarrate, Estibaliz Mateo, Katherine Miranda-Cadena, Amaia Lasagabaster
Summary: This study reported the isolation and characterization of 304 Campylobacter specific bacteriophage isolates from broiler and swine sources. The most promising 59 campylophage candidates were selected based on their lytic activity, and RAPD-PCR technique was found to be effective only for Group II campylophages. Bacteriophage isolates within the same genotype displayed different host ranges, leading to the differentiation of 13 campylophages from Group II and 8 from Group III.
Article
Microbiology
I-Chen Li, Rayean Wu, Chung-Wen Hu, Keh-Ming Wu, Zeng-Weng Chen, Chung-Hsi Chou
Summary: Studies have shown that whole genome sequencing (WGS) has the highest resolution in distinguishing Salmonella enterica serovar Schwarzengrund strains compared to traditional methods like pulsed-field gel electrophoresis (PFGE), clustered regular interspaced short palindromic repeats (CRISPR), and multilocus sequence typing (MLST).
Article
Biotechnology & Applied Microbiology
Nicol Strakova, Hana Michova, Ekaterina Shagieva, Petra Ovesna, Renata Karpiskova, Katerina Demnerova
Summary: The study aimed to compare the effectiveness of three genotyping methods (MLST, PFGE, and mP-BIT) in tracing the pathogen responsible for the most common foodborne illness. The results showed that MLST is the optimal tool for genotyping Campylobacter jejuni. Additionally, the study compared two gene sequence-based prediction methods for antimicrobial resistance and found that both methods can be used.
FOLIA MICROBIOLOGICA
(2023)
Article
Microbiology
Codruta-Romanita Usein, Mihaela Oprea, Adriana Simona Ciontea, Sorin Dinu, Daniela Cristea, Lavinia Cipriana Zota, Saara Kotila
Summary: This study investigated the genetic diversity and antibiotic susceptibility of Salmonella Enteritidis strains in Romania from 2016 to 2020, comparing them to international outbreaks. Through genotyping and whole genome sequencing, multiple MLVA genotypes were identified, along with antibiotic resistance patterns. The study revealed a connection between Romanian and international strains, suggesting the potential use of this integrative approach in public health practice at a national level.
Article
Fisheries
Muhammed Duman, Noemi Bujan, Soner Altun, Jesus L. Romalde, Izzet Burcin Saticioglu
Summary: This study characterized Vibrio isolates from Turkish fish farms, PubMLST database, and GenBank using multilocus sequence analysis and typing. They found high diversity and genetic interactions within the Vibrio population. 17 new sequence types were discovered, indicating frequent genetic recombination. These findings help understand differences in Vibrio infections in fish and provide insights for effective measures in aquaculture.
Article
Microbiology
Luiza Pinheiro-Hubinger, Danilo Flavio Moraes Riboli, Ligia Maria Abraao, Eliane Patricia Lino Pereira Franchi, Maria de Lourdes Ribeiro de Souza da Cunha
Summary: This study compared the clonality and SCCmec type of CoNS isolates from different sources in the same community, revealing high clonal diversity in S. epidermidis and circulation of some S. haemolyticus clones in both hospital and community. The presence of different SCCmec types within the same cluster indicated high diversity, suggesting SCCmec may not be a good marker of clonality in these bacteria.
Article
Infectious Diseases
Beatriz Rojo-Bezares, Laura Toca, Jose Manuel Azcona-Gutierrez, Nerea Ortega-Unanue, Paula Toledano, Yolanda Saenz
Summary: This study aimed to characterize the antibiotic resistance, virulence, and genetic diversity among invasive and non-invasive Streptococcus dysgalactiae subsp. equisimilis (SDSE) isolates. The researchers found a high diversity of circulating genetic lineages among SDSE isolates, and identified various resistance and virulence genes associated with different emm types. Invasive infections of SDSE were most frequent in elderly patients, and half of the isolates were resistant to at least one antibiotic tested.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2021)
Article
Infectious Diseases
Malgorzata Murawska, Monika Sypecka, Justyna Bartosik, Ewelina Kwiecien, Magdalena Rzewuska, Agnieszka Salamaszynska-Guz
Summary: Research suggests that dogs may serve as a reservoir for diverse, potentially virulent, and antimicrobial-resistant Campylobacter strains.
Article
Biophysics
Gloria Alessia, Luca Candeloro, Laura Wegher, Domenico Robbe, Augusto Carluccio, Contri Alberto
Summary: The study found limited seasonal effects on the semen quality of Brown Swiss bulls, while Belgian Blue bulls showed lower semen quality between July and November, with different timing for each seminal parameter. This seasonal effect on Belgian Blue semen parameters appears to be related to the Temperature-Humidity Index (THI). The data suggests that temperature and humidity, reflected in THI, could significantly impact semen quality in Belgian Blue bulls, but not in Brown Swiss bulls.
INTERNATIONAL JOURNAL OF BIOMETEOROLOGY
(2021)
Article
Microbiology
Margherita Perilli, Silvia Scattolini, Gianluca Ciro Telera, Alessandra Cornacchia, Patrizia Tucci, Flavio Sacchini, Massimo Sericola, Romina Romantini, Francesca Marotta, Andrea Di Provvido, Francesco Pomilio, Fabrizio De Massis
Summary: This study investigated the prevalence of different Salmonella serotypes in the Abruzzo and Molise Regions of Italy between 2015 and 2020. The results showed that broilers and breeding/fattening turkeys are important carriers of Salmonella, particularly the dangerous serotype Salmonella Infantis.
Article
Biotechnology & Applied Microbiology
Federica Palma, Iolanda Mangone, Anna Janowicz, Alexandra Moura, Alexandra Chiaverini, Marina Torresi, Giuliano Garofolo, Alexis Criscuolo, Sylvain Brisse, Adriano Di Pasquale, Cesare Camma, Nicolas Radomski
Summary: This study identified parameters influencing the precision of cgMLST profiles in Listeria monocytogenes, highlighting the impact of genetic background, cgMLST workflows, completeness, depth, and breadth of coverage on precision. All tested workflows performed well at >= 40X depth of coverage, showing consistent cluster definitions using a reference cut-off of <= 7 allele differences. The research suggests that bioinformatics workflows dedicated to cgMLST allele calling are robust when paired-end reads are of high quality and sequencing depth is sufficient.
Article
Veterinary Sciences
Lisa Di Marcantonio, Francesca Marotta, Michele Podaliri Vulpiani, Quixi Sonntag, Luigi Iannetti, Anna Janowicz, Gabriella Di Serafino, Elisabetta Di Giannatale, Giuliano Garofolo
Summary: The present study assessed the modulation of cecal microbiota and correlations with Campylobacter colonization and animal welfare status. The results suggested a different microbial composition and diversity in the analyzed groups.
RESEARCH IN VETERINARY SCIENCE
(2022)
Article
Microbiology
Lisa Di Marcantonio, Romina Romantini, Francesca Marotta, Alexandra Chiaverini, Katiuscia Zilli, Anna Abass, Elisabetta Di Giannatale, Giuliano Garofolo, Anna Janowicz
Summary: Salmonella enterica serovar Infantis strains in the Abruzzo and Molise regions of Italy have shown an increasing trend in antimicrobial resistance, particularly against multiple antibiotics and extended-spectrum beta-lactamase (ESBL) genes. These strains are closely related to earlier Italian strains and predominantly carry the pESI-like plasmid. There has been a significant increase in the number of identified antimicrobial resistance genes in strains isolated after 2011.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Alessandra Alessiani, Elisa Goffredo, Maria Mancini, Gilda Occhiochiuso, Simona Faleo, Antonella Didonna, Rita Fischetto, Federica Suglia, Danila De Vito, Antonella Stallone, Luigi D'Attoli, Adelia Donatiello
Summary: Salmonella enterica is a common cause of foodborne infection worldwide, especially in Italy. A study on the antimicrobial resistance of Salmonella in Apulia and Basilicata provides a focused perspective on the region, revealing high resistance to several antibiotics and the need for further research and action in antibiotic management.
Article
Microbiology
Mostafa Y. Abdel-Glil, Prasad Thomas, Christian Brandt, Falk Melzer, Anbazhagan Subbaiyan, Pallab Chaudhuri, Dag Harmsen, Keith A. Jolley, Anna Janowicz, Giuliano Garofolo, Heinrich Neubauer, Mathias W. Pletz
Summary: This study describes a novel Brucella-wide cgMLST scheme that is applicable in Brucella molecular epidemiology and helps in accurately tracking and thus controlling the sources of infection.
JOURNAL OF CLINICAL MICROBIOLOGY
(2022)
Article
Infectious Diseases
Flavia Riccardo, Antonino Bella, Federica Monaco, Federica Ferraro, Daniele Petrone, Alberto Mateo-Urdiales, Xanthi D. Andrianou, Martina Del Manso, Giulietta Venturi, Claudia Fortuna, Marco Di Luca, Francesco Severini, Maria Grazia Caporali, Daniela Morelli, Federica Iapaolo, Ilaria Pati, Letizia Lombardini, Tamas Bakonyi, Olivia Alexandra, Patrizio Pezzotti, Maria Gabriella Perrotta, Francesco Maraglino, Giovanni Rezza, Anna Teresa Palamara
Summary: Similar to 2018, the vector season in Italy in 2022 witnessed an early onset of West Nile virus (WNV) transmission, resulting in a rapid increase in human infections. This article describes the epidemiology of human infections and the surveillance of animals and vectors for WNV, comparing the data from June and July 2022 with the same period in 2018.
Article
Microbiology
Maria Emanuela Mancini, Alessandra Alessiani, Adelia Donatiello, Antonella Didonna, Luigi D'Attoli, Simona Faleo, Gilda Occhiochiuso, Francesco Carella, Pietro Di Taranto, Lorenzo Pace, Valeria Rondinone, Annita Maria Damato, Rosa Coppola, Carmine Pedarra, Elisa Goffredo
Summary: This study aimed to determine the prevalence and antibiotic susceptibility of Salmonella spp. and Vibrio spp. in bivalve molluscs and the environmental multidrug resistance (MDR). The results showed one Salmonella strain with multidrug resistance and 41.3% of the Vibrio strains with resistance to certain antibiotics. Continuous monitoring and establishment of interpretative criteria are recommended.
Article
Food Science & Technology
Monika Ziomek, Michal Gondek, Beatrice Torracca, Francesca Marotta, Giuliano Garofolo, Kinga Wieczorek, Katarzyna Michalak, Filippo Fratini, Francesca Pedonese
Summary: A study examined the presence of Campylobacter species in wild boars in Tuscany to determine their contribution to human infection through the food chain. The study found that 44.56% of the animals and various samples were contaminated with Campylobacter spp., with C. coli and C. lanienae being the dominant species. The study emphasizes the importance of providing food safety information to hunters and consumers due to the contamination of meat and liver with Campylobacter spp.
Article
Infectious Diseases
Antonio Cocco, Alessandra Alessiani, Romolo Salini, Federica Iapaolo, Daniela Averaimo, Cinzia Pompilii, Giovanni Foschi, Fabio Bellucci, Filomena Iannino, Paolo Dalla Villa, Anna Janowicz, Marco Caporale
Summary: This study assessed antimicrobial resistance (AMR) in Escherichia coli isolated from dogs in Italian shelters and correlated the resistance patterns with animal welfare. Various pathogens with zoonotic potential were also identified in sheltered dogs. The results showed a correlation between AMR and animal welfare scores, emphasizing the importance of good shelter management in reducing the occurrence of AMR in dogs.
Article
Microbiology
Elisa Mancuso, Marco Di Domenico, Luigina Di Gialleonardo, Michela Menegon, Luciano Toma, Marco Di Luca, Francesca Casale, Guido Di Donato, Laura D'Onofrio, Angela De Rosa, Sara Riello, Andrea Ferri, Lorenzo Serra, Federica Monaco
Summary: The role of migratory birds in spreading ticks and tick-borne pathogens from Africa to Europe is becoming more evident. A study was conducted to assess the introduction of exotic ticks carried by migratory birds into Italy, as well as to detect the presence of Rickettsia species and Coxiella burnetii they may harbor. The findings confirm the role of migratory birds in the spread of ticks and tick-borne pathogens, highlighting the need for continuous surveillance to monitor the potential emergence of new diseases in Europe.
Article
Food Science & Technology
Francesca Marotta, Anna Janowicz, Romina Romantini, Lisa Di Marcantonio, Federica Di Timoteo, Teresa Romualdi, Katiuscia Zilli, Lisa Barco, Mario D'Incau, Iolanda Mangone, Francesca Cito, Marco Di Domenico, Francesco Pomilio, Lucilla Ricci, Giuliano Garofolo
Summary: This study evaluated the genetic diversity and antibiotic resistance of C. jejuni and C. coli in Italian poultry. The results showed increased resistance to quinolones, fluoroquinolones, macrolides, and the emergence of multidrug resistant strains in C. coli. The study also identified two prevalent genotypes in Italian poultry farms.
Article
Veterinary Sciences
Filomena Iannino, Guido Di Donato, Stefania Salucci, Enzo Ruggieri, Giacomo Vincifori, Maria Luisa Danzetta, Paolo Dalla Villa, Elisabetta Di Giannatale, Giulia Lotti, Fabrizio De Massis
Summary: This study aimed to investigate the carriage, infection, and antimicrobial resistance of Campylobacter in household and shelter dogs in Italy, as well as assess the risk of transmission to humans. The results showed that dogs pose a certain risk in the transmission of Campylobacteriosis to humans, and resistant strains were detected. Therefore, controlling the prevalence of Campylobacter in dogs is crucial for reducing transmission to humans.
VETERINARIA ITALIANA
(2022)
Article
Multidisciplinary Sciences
Francesca Cecchi, Maria Chiara Fabbri, Lara Tinacci, Roberta Nuvoloni, Francesca Marotta, Lisa Di Marcantonio, Giovanni Cilia, Fabio Macchioni, Andrea Armani, Filippo Fratini, Francesca Pedonese
Summary: This study investigated the genetic resistance to Campylobacter coli and Campylobacter jejuni in wild boar using both STR analysis and GWAS. The results showed no significant association between STRs and SNPs and Campylobacter positivity, and no candidate genes directly or indirectly involved in the disease development were identified.
RENDICONTI LINCEI-SCIENZE FISICHE E NATURALI
(2022)