Article
Chemistry, Multidisciplinary
J. Grabowska, A. J. Affandi, D. van Dinther, M. K. Nijen Twilhaar, K. Olesek, L. Hoogterp, M. Ambrosini, D. A. M. Heijnen, L. Klaase, A. Hidalgo, K. Asano, P. R. Crocker, G. Storm, Y. van Kooyk, J. M. M. den Haan
Summary: In this study, liposomes containing GM3 were shown to efficiently target CD169(+) macrophages, inducing specific immune responses including antigen-specific CD8(+) and CD4(+) T cell as well as B cell responses. Administration of GM3 liposomes containing ovalbumin resulted in delayed tumor growth and improved survival in B16-OVA tumor bearing mice. The study also highlighted the essential role of CD169(+) macrophages and cDC1s in inducing CD8(+) T cell immunity through liposomal vaccination.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Biochemistry & Molecular Biology
Guangwei Han, Haotian Yang, Huiling Xu, Suya Zheng, Yingxiang Li, Zehui Liu, Fang He
Summary: Novel antiviral peptides derived from pCD163 were developed, which showed inhibitory effects on PRRSV of different lineages and modulated the NF-kappa B pathway. These findings deepen the understanding of the interaction between PRRSV and pCD163 receptor, and provide alternative universal antiviral strategies against PRRSV.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biochemistry & Molecular Biology
Zhuofan Deng, Shengkun Zhang, Meiqi Sun, Haotian Yang, Ying Lu, Maopeng Wang, Weihuan Fang, Fushan Shi, Fang He
Summary: In this study, a phage display VHH library was constructed to select nanoantibodies against porcine CD163 receptor. Seven CD163-specific nanoantibodies were identified and showed strong affinity to CD163 receptor and excellent antiviral activity. The nanoantibodies mainly exerted antiviral functions by interfering with virus attachment stage and decreasing the transcription of CD163. The findings provide insights into the biofunction of CD163 in antiviral infection and the development of broad-spectrum strategies against PRRSV.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Immunology
Jiexiong Xie, Nick Vereecke, Sebastiaan Theuns, Dayoung Oh, Nathalie Vanderheijden, Ivan Trus, Jannes Sauer, Philip Vyt, Caroline Bonckaert, Christian Lalonde, Chantale Provost, Carl A. Gagnon, Hans Nauwynck
Summary: Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) exhibits a highly restricted cellular tropism, with different cell lines showing varying susceptibility to infection. PK15(Sn-CD163) and PK15(S10-CD163) cells are more suitable for virus isolation and propagation compared to MARC-145/MARC-145(Sn) cells due to their close resemblance to target macrophages in vivo. Further studies on PRRSV protein residues potentially related to cell tropism are warranted.
Article
Immunology
Rika Wahyuningtyas, Yin-Siew Lai, Mei-Li Wu, Hsin-Wei Chen, Wen-Bin Chung, Hso-Chi Chaung, Ko-Tung Chang
Summary: The study indicates that A1 can reverse the polarization status of PAMs towards pro-inflammatory phenotypes, reducing CD163 expression for viral entry and increasing Th1 response. Therefore, A1 is considered a potential vaccine for PRRSV infection.
Article
Biology
Jamie Moore-Fried, Mahinder Paul, Zhixin Jing, David Fooksman, Gregoire Lauvau
Summary: This study reveals the critical roles of CD169(+) macrophages (MPs) and TLR7 sensing in initiating type I interferon (IFN) production by plasmacytoid dendritic cells (pDCs) in severe malaria. TLR7 sensing and chemotaxis are required for pDC arrest and functional communication with CD169(+) MPs, while STING sensing in CD169(+) MPs controls pDC initiation of type I IFN production and their clustering and egress from the bone marrow.
Article
Biotechnology & Applied Microbiology
Ana M. M. Stoian, Raymond R. R. Rowland, Alberto Brandariz-Nunez
Summary: This study identified the critical regions within the SRCR5 domain that are responsible for PRRSV infection by introducing different mutants. The results showed that there are differences in the recognition of CD163 between PRRSV-1 and PRRSV-2, but all mutations affecting infection are localized to a similar region on the same face of SRCR5.
JOURNAL OF GENERAL VIROLOGY
(2022)
Review
Microbiology
Xiaoxiao Zhang, Chunhe Guo
Summary: The Porcine reproductive and respiratory syndrome virus (PRRSV) is a major concern in the pig industry, with limited tools available to combat it. CD163 molecule is identified as the key receptor for PRRSV infection, and targeting it could lead to novel antiviral molecules and gene-modified animals for protection against PRRSV.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Immunology
Joanna Grabowska, Valentine Leopold, Katarzyna Olesek, Maarten K. Nijen Twilhaar, Alsya J. Affandi, Mieke C. Brouwer, Ilse Jongerius, Admar Verschoor, Cees van Kooten, Yvette van Kooyk, Gert Storm, Cornelis van 't Veer, Joke M. M. den Haan
Summary: Platelets play a crucial role in inflammation and immunity, interacting with immune cells to activate adaptive immune responses. This study demonstrates that platelets associate with liposomes and interact with specific receptors, enhancing liposome internalization by dendritic cells and increasing CD8(+) T cell immune responses. Depletion of platelets significantly diminishes antigen-specific CD8(+) T cell responses, highlighting the importance of platelets in liposomal vaccination-induced immune responses.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Shunxian Zhang, Xiaoqin Li, Li Zhu, Siqi Ming, Hongli Wang, Jing Xie, Lu Ren, Jing Huang, Defeng Liang, Liya Xiong, Yuesheng Wang, Dan Zhang, Sitang Gong, Yongjian Wu, Lanlan Geng
Summary: The study revealed that CD163(+)M phi in colorectal polyps may promote polyp progression by inhibiting the local T cell response.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Review
Immunology
Jesus Hernandez, Yanli Li, Enric Mateu
Summary: Dendritic cells in pigs consist of three types, with different pathogens having the ability to infect these cells, especially Porcine Reproductive and Respiratory Syndrome Virus (PRRSV). Research has shown that some dendritic cells are refractory to PRRSV infection, while others may have different responses depending on the type of PRRSV involved.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Virology
Jiaqi Zhu, Xin He, Denzil Bernard, Jianing Shen, Yue Su, Andrew Molek, Brianna Issacs, Neha Mishra, Xiuchun Tian, Antonio Garmendia, Young Tang
Summary: Porcine reproductive and respiratory syndrome virus (PRRSV) causes economic losses to the swine industry worldwide. Current vaccines cannot provide cross protection against different strains and there are no effective treatments available. New small molecules have been identified that can inhibit PRRSV infection by targeting the specific receptor CD163.
JOURNAL OF VIROLOGY
(2023)
Article
Biology
Stephen Meek, Tom Watson, Lel Eory, Gus McFarlane, Felicity J. Wynne, Stephen McCleary, Laura E. M. Dunn, Emily M. Charlton, Chloe Craig, Barbara Shih, Tim Regan, Ryan Taylor, Linda Sutherland, Anton Gossner, Cosmin Chintoan-Uta, Sarah Fletcher, Philippa M. Beard, Musa A. Hassan, Finn Grey, Jayne C. Hope, Mark P. Stevens, Monika Nowak-Imialek, Heiner Niemann, Pablo J. Ross, Christine Tait-Burkard, Sarah M. Brown, Lucas Lefevre, Gerard Thomson, Barry W. McColl, Alistair B. Lawrence, Alan L. Archibald, Falko Steinbach, Helen R. Crooke, Xuefei Gao, Pentao Liu, Tom Burdon
Summary: This study investigates the potential of porcine pluripotent stem cells to provide a limitless source of macrophages, demonstrating their molecular and functional characteristics, as well as their susceptibility to infection by pig pathogens. The study also shows the successful application of CRISPR/Cas9 gene editing in these cells, presenting new opportunities for researching host-pathogen interactions in pigs.
Article
Microbiology
Na Li, Ke Huang, Yujing Chen, Zhengyang Huang, Yiyi Zhang, ChaoLiang Leng, Yangkun Liu, Jianzhou Shi, Shuqi Xiao, Lunguang Yao
Summary: This study revealed that ssc-miR-124a plays a significant regulatory role in PRRSV infection, inhibiting virus replication and decreasing CD163 protein levels. These findings provide new insights and potential antiviral strategies against PRRSV infection in the future.
VETERINARY MICROBIOLOGY
(2021)
Article
Immunology
Christina Lisk, Rachel Yuen, Jeff Kuniholm, Danielle Antos, Michael L. Reiser, Lee M. Wetzler
Summary: Vaccines are crucial for public health, yet some infectious diseases lack effective vaccines. Utilizing Toll-like receptor (TLR) ligand-based adjuvants has been shown to enhance vaccine efficacy, particularly through increased antibody production. Subcapsular macrophages play a unique role in vaccine-induced antibody production.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Tiong Kit Tan, Pramila Rijal, Rolle Rahikainen, Anthony H. Keeble, Lisa Schimanski, Saira Hussain, Ruth Harvey, Jack W. P. Hayes, Jane C. Edwards, Rebecca K. McLean, Veronica Martini, Miriam Pedrera, Nazia Thakur, Carina Conceicao, Isabelle Dietrich, Holly Shelton, Anna Ludi, Ginette Wilsden, Clare Browning, Adrian K. Zagrajek, Dagmara Bialy, Sushant Bhat, Phoebe Stevenson-Leggett, Philippa Hollinghurst, Matthew Tully, Katy Moffat, Chris Chiu, Ryan Waters, Ashley Gray, Mehreen Azhar, Valerie Mioulet, Joseph Newman, Amin S. Asfor, Alison Burman, Sylvia Crossley, John A. Hammond, Elma Tchilian, Bryan Charleston, Dalan Bailey, Tobias J. Tuthill, Simon P. Graham, Helen M. E. Duyvesteyn, Tomas Malinauskas, Jiandong Huo, Julia A. Tree, Karen R. Buttigieg, Raymond J. Owens, Miles W. Carroll, Rodney S. Daniels, John W. McCauley, David I. Stuart, Kuan-Ying A. Huang, Mark Howarth, Alain R. Townsend
Summary: This study introduces a potential vaccine candidate against SARS-CoV-2, utilizing a synthetic virus-like particle platform to present the receptor-binding domain of the virus spike protein. The vaccine induces strong neutralizing antibody responses in mice and pigs, demonstrating thermostability and the potential for global distribution.
NATURE COMMUNICATIONS
(2021)
Article
Microbiology
Kerstin Wernike, Ilona Reimann, Ashley C. Banyard, Franziska Kraatz, S. Anna La Rocca, Bernd Hoffmann, Sarah McGowan, Silke Hechinger, Bhudipa Choudhury, Andrea Aebischer, Falko Steinbach, Martin Beer
Summary: Variants of the Schmallenberg virus with amino acid sequence mutations in the N-terminal main immunogenic domain of glycoprotein Gc are able to evade neutralizing antibodies, indicating these variants are unable to spread in the special environment of a developing fetus. This has implications for understanding the immune evasion mechanisms of the virus and highlights the importance of excluding mutant strains in outbreak investigations.
Article
Microbiology
Barbara Holzer, Pramila Rijal, Adam McNee, Basudev Paudyal, Veronica Martini, Becky Clark, Tanuja Manjegowda, Francisco J. Salguero, Emily Bessell, John C. Schwartz, Katy Moffat, Miriam Pedrera, Simon P. Graham, Alistair Noble, Marie Bonnet-Di Placido, Roberto M. La Ragione, William Mwangi, Peter Beverley, John W. McCauley, Rodney S. Daniels, John A. Hammond, Alain R. Townsend, Elma Tchilian
Summary: Pigs, natural hosts for the same subtypes of influenza A viruses as humans, generate monoclonal antibodies that target the same immunodominant epitopes as humans. These pig mAbs significantly reduce disease severity in influenza virus infection, indicating the potential for pigs as a valuable model for antibody therapy and vaccine recommendations for humans.
Article
Immunology
Jordan E. Young, Cheryl M. T. Dvorak, Simon P. Graham, Michael P. Murtaugh
Summary: This study isolated PRRSV-specific B-cell populations from pigs with a broad neutralizing antibody response using a reverse vaccinology approach, and identified the significant role of the GP5 protein in PRRSV neutralization.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ferran Soldevila, Jane C. Edwards, Simon P. Graham, Helen R. Crooke, Dirk Werling, Falko Steinbach
Summary: The study found that the C-strain vaccine caused changes in the myeloid cell compartment of the tonsil, leading to an increase and activation of specific immune cells. Additionally, the C-strain vaccine also promoted the activation of conventional dendritic cells 1 (cDC1) in the tonsil, which coincided with the induction of CSFV-specific CD8 T cell responses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Infectious Diseases
Akbar Dastjerdi, S. Anna La Rocca, Siva Karuna, Christopher Finnegan, Julie Peake, Falko Steinbach
Summary: Schmallenberg orthobunyavirus (SBV) is a virus transmitted through midges and can be transiently shed in low to minute quantities in semen post-infection. Despite its low impact on the dairy industry, some countries require bull semen batches to be tested for SBV RNA residues before import.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Biology
Stephen Meek, Tom Watson, Lel Eory, Gus McFarlane, Felicity J. Wynne, Stephen McCleary, Laura E. M. Dunn, Emily M. Charlton, Chloe Craig, Barbara Shih, Tim Regan, Ryan Taylor, Linda Sutherland, Anton Gossner, Cosmin Chintoan-Uta, Sarah Fletcher, Philippa M. Beard, Musa A. Hassan, Finn Grey, Jayne C. Hope, Mark P. Stevens, Monika Nowak-Imialek, Heiner Niemann, Pablo J. Ross, Christine Tait-Burkard, Sarah M. Brown, Lucas Lefevre, Gerard Thomson, Barry W. McColl, Alistair B. Lawrence, Alan L. Archibald, Falko Steinbach, Helen R. Crooke, Xuefei Gao, Pentao Liu, Tom Burdon
Summary: This study investigates the potential of porcine pluripotent stem cells to provide a limitless source of macrophages, demonstrating their molecular and functional characteristics, as well as their susceptibility to infection by pig pathogens. The study also shows the successful application of CRISPR/Cas9 gene editing in these cells, presenting new opportunities for researching host-pathogen interactions in pigs.
Article
Microbiology
Giulia Franzoni, Susanna Zinellu, Tania Carta, Chiara Grazia De Ciucis, Floriana Fruscione, Antonio Anfossi, Mauro Ledda, Simon P. Graham, Silvia Dei Giudici, Elisabetta Razzuoli, Annalisa Oggiano
Summary: African swine fever viruses primarily target porcine macrophages, and anti-inflammatory cytokines IL-10 and TGF-beta have little impact on the interaction between macrophages and ASFV.
Article
Chemistry, Analytical
H. F. El Sharif, S. R. Dennison, M. Tully, S. Crossley, W. Mwangi, D. Bailey, S. P. Graham, S. M. Reddy
Summary: This study investigates the use of electropolymerized molecularly imprinted polymers (E-MIPs) for the selective recognition of SARS-CoV-2 whole virus. The results show that E-MIPs exhibit selectivity for SARS-CoV-2, allowing for rapid detection and effective capture of the virus in real patient saliva samples.
ANALYTICA CHIMICA ACTA
(2022)
Article
Public, Environmental & Occupational Health
Rebecca K. McLean, Simon P. Graham
Summary: Pig production is rapidly growing globally, especially in Asia and Africa, but it has also led to the emergence and spread of infectious diseases. Pigs play a crucial role as intermediate/amplifying hosts for various zoonotic viruses, including influenza, Japanese encephalitis, Nipah, and coronaviruses. Multivalent vaccination of pigs can safeguard human health indirectly by protecting the health of pigs.
Article
Virology
Lynnette C. Goatley, Rachel H. Nash, Catherine Andrews, Zoe Hargreaves, Priscilla Tng, Ana Luisa Reis, Simon P. Graham, Christopher L. Netherton
Summary: The Babraham large white pig, a highly inbred line, may serve as a valuable tool for studying the protective immune responses to African swine fever virus. However, the response to vaccination differs between inbred and outbred pigs, with inbred pigs showing diminished immune responses and lack of protection after challenge. This study found that the recovery of Babraham pigs weakly correlated with antibody responses, whereas protective responses in outbred animals were more closely associated with T-cell response.
Article
Virology
Giulia Franzoni, Susanna Zinellu, Elisabetta Razzuoli, Lorena Mura, Chiara G. De Ciucis, Livia De Paolis, Tania Carta, Antonio G. Anfossi, Simon P. Graham, Bernardo Chessa, Silvia Dei Giudici, Annalisa Oggiano
Summary: This study investigated the role of a synthetic diacylated lipopeptide (Mag-Pam2Cys_P48) in modulating macrophage responses against African swine fever virus (ASFV) and found limited efficacy of Toll-like receptor 2 (TLR2) agonist against virulent strains of ASFV.
Article
Immunology
Ellie Hayhurst, Emily Rose, Miriam Pedrera, Jane C. Edwards, Natalia Kotynska, Daisy Grainger, Yashar Sadigh, John Flannery, Ludo Bonnet, Ritwik Ritwik, Pawan Dulal, M. Keith Howard, Simon P. Graham
Summary: Solid dose vaccine formulation offers potential advantages over traditional liquid formulations, providing safe, rapid, and error-free administration for both human and animal health. It can be adapted for different vaccine formats, including live attenuated vaccines.
Article
Veterinary Sciences
Meera Prajapati, Madhav Prasad Acharya, Prakash Yadav, Jean-Pierre Frossard
Summary: This study evaluates the prevalence of PRRSV antibodies in pigs in different districts of Nepal and assesses the farm characteristics and knowledge level of farmers. The results show the presence of PRRSV antibodies in pigs and highlight the importance of improving biosecurity measures, management practices, and farmer knowledge for disease control and prevention.
VETERINARY MEDICINE AND SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Giulia Franzoni, Lorena Mura, Elisabetta Razzuoli, Chiara Grazia De Ciucis, Floriana Fruscione, Filippo Dell'Anno, Susanna Zinellu, Tania Carta, Antonio G. G. Anfossi, Silvia Dei Giudici, Simon P. P. Graham, Annalisa Oggiano
Summary: Swine are gaining attention as a valuable biomedical model due to their immunological similarities with humans, but the polarization of porcine macrophages has not been well-studied. In this study, we investigated how porcine monocyte-derived macrophages respond to different activation stimuli. Our results show that IFN-gamma and LPS induce a proinflammatory phenotype, while IL-4, IL-10, TGF-beta, and dexamethasone trigger distinct M2-related phenotypes. These findings contribute to our understanding of porcine macrophage plasticity and reveal some unique characteristics in this species.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)