Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 135, Issue -, Pages 556-564Publisher
ELSEVIER
DOI: 10.1016/j.colsurfb.2015.07.079
Keywords
Micelle-based vaccine; Adjuvant; Immune response; Germinal center
Funding
- National Natural Science Foundation of China [51303136, 51403159]
- Science and Technology Bureau of Wenzhou city [Y20140141]
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Nanoparticles have been proven to be an effective vaccine delivery system that can boost immune responses to subunit vaccines. Herein, we developed and characterized a cationic polymeric polyethylene glycol(2000)-poly epsilon-caprolactone(2000)-polyethylenimine(2000) (mPEG(2000)-PCL2000-g-PEI2000) micelle as a potent vaccine delivery system to boost the immune response in vivo. The micelles that we developed exhibited great antigen-loading capability and minimal cytotoxicity in vitro. Meanwhile, micelles facilitated OVA antigen uptake by dendritic cells both in vitro and in vivo. More importantly, a micelle-formulated OVA vaccine could significantly promote anti-OVA antibody production by 190-fold and potently enhance T cell proliferation and the secretion of IL-5 and IFN-gamma. We attributed these effects to its ability to promote antigen uptake, antigen deposition, and germinal center formation. In conclusion, the mPEG(2000)-PCL2000-PEI2000 micelle that we developed has potential as potent vaccine delivery system to induce Th2 immune response. (C) 2015 Elsevier B.V. All rights reserved.
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