Journal
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
Volume 484, Issue -, Pages 345-353Publisher
ELSEVIER
DOI: 10.1016/j.colsurfa.2015.07.052
Keywords
Lysozyme; IL; Coacervate; Iso-emissive points; Circular dichroism
Categories
Funding
- UGC, Govt. of India
- Jadavpur University
Ask authors/readers for more resources
The interactions of the surface active ionic liquid (IL), 1-butyl-3-methylimidazolium octylsulfate [BMIM] [OS], with lysozyme were studied using tensiometry, turbidimetry, absorbance, fluorescence spectra, circular dichroism (CD), and Fourier transformed infra red (FTIR) techniques in phosphate buffer medium (pH 6 and 8) at 298 K. In the studied media coacervate was formed and the coacervation process depended on protein concentration. Isoemissive points were observed in emission spectra of lysozyme with IL The isoemissive wavelength depended on the pH of the solution and also on [lysozyme]. The denaturation of lysozyme depended on the medium pH and the added [IL] as revealed from the CD and FTIR measurements. Molecular docking has provided information of the region of the lysozyme surface where the IL started to bind with it. (C) 2015 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available