Journal
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
Volume 45, Issue 6, Pages 1165-1171Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/21691401.2016.1203795
Keywords
Antibacterial; antioxidant; green synthesis; Prunus japonica; silver nanoparticles
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Funding
- Hanseo University Intramural Research Grant
- National Research Foundation of Korea (NRF) - Ministry of Science, ICT, and Future Planning [2014-004694]
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Low cost and eco-friendly green synthesis of silver nanoparticles (AgNPs) from silver nitrate (AgNO3) using Prunus japonica leaves extract as reducing agent by a simple method at room temperature. The biosynthesized nanoparticles (NPs) were characterized by UV-Vis, tunneling electron microscopy (HR-TEM), scanning electron microscopy (SEM) coupled with X-ray energy dispersive spectrophotometer (EDAX), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR). In UV-Vis spectroscopy results, the (max) was observed at 441nm. The AgNPs synthesized were spherical, hexagonal, and irregular in shapes. The EDAX and XRD spectrum confirmed the presence of silver ions and crystalline nature of synthesized AgNPs. FTIR showed the functional groups such as C=O, N-H and C-N groups involved in the reduction of Ag(+)to Ag. 2, 2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay was performed and it showed the percentage inhibition in concentration-dependent manner. The synthesized AgNPs showed antibacterial activity against Escherichia coli, Proteus vulgaris, Staphylococcus aureus and Bacillus cereus to different extents and the higher activity was observed in Proteus vulgaris.
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