Journal
STEM CELLS TRANSLATIONAL MEDICINE
Volume 5, Issue 4, Pages 451-463Publisher
WILEY
DOI: 10.5966/sctm.2015-0155
Keywords
Pregnancy; Stem cells; Placenta; Intrauterine growth restriction; Mesenchymal stromal cells
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Funding
- Fondazione Giorgio Pardi
- Italian Ministry of University and Research PRIN [20102chst5_005]
- Associazione Amici del Centro Dino Ferrari
- Associazione Amici di Emanuele
- Fondazione Opsis Onlus
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Human placental mesenchymal stromal cells (pMSCs) have, never been investigated in intrauterine growth restriction (IUGR). We characterized cells isolated from placental membranes and the basal disc of six IUGR and five physiological placentas. Cell viability and proliferation were assessed every 7 days during a 6-week culture. Expression of hematopoietic, stem, endothelial, and mesenchymal markers was evaluated by flow cytometry. We characterized the multipotency of pMSCs and the expression of genes involved in mitochondria! content and function. Cell viability was high in all samples, and proliferation rate was lower in IUGR compared with control cells. All samples presented a starting heterogeneous population, shifting during culture toward homogeneity for mesenchymal markers and occurring earlier in IUGR than in controls. In vitro multipotency of IUGR-derived pMSCs was restricted because their capacity for adipocyte differentiation was increased, whereas their ability to differentiate toward endothelial cell lineage was decreased. Mitochondrial content and function were higher in IUGR pMSCs than controls, possibly indicating a shift from anaerobic to aerobic metabolism, with the loss of the metabolic characteristics that are typical of undifferentiated multipotent cells.
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