Review
Chemistry, Multidisciplinary
Anilkumar Yadav, Sagnik Ghosh, Archana Samanta, Jit Pal, Rajiv K. Srivastava
Summary: The review explores the role of PCL and its 3D scaffolds in tissue engineering, focusing on the use of HIPE templating in the fabrication of PCL scaffolds, as well as the challenges and potential for expansion of the technique. Additionally, it discusses how HIPE templating can be coupled with advanced fabrication methods such as 3D printing or electrospinning.
CHEMICAL COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Fan Liu, Honglei Kang, Zhiwei Liu, Siyang Jin, Guoping Yan, Yunlong Sun, Feng Li, Haifei Zhan, Yuantong Gu
Summary: 3D printed biodegradable polymeric scaffolds made of PCL/HA composites show great potential in promoting cell proliferation and bone tissue regeneration due to good biodegradability and biocompatibility, as demonstrated in in vitro and in vivo experiments. Additionally, the multifunctional PCL/HA/DOX scaffolds have the capacity to enhance bone repair and potentially inhibit tumor cells, indicating their promising application in bone repair.
Article
Polymer Science
Sangbae Park, Jae Eun Kim, Jinsub Han, Seung Jeong, Jae Woon Lim, Myung Chul Lee, Hyunmok Son, Hong Bae Kim, Yun-Hoon Choung, Hoon Seonwoo, Jong Hoon Chung, Kyoung-Je Jang
Summary: This study modified the surface characteristics of 3D-printed PCL/HA scaffolds using O2 plasma and alkali treatment, and found that the alkali treatment can better expose hydroxyapatite particles in the scaffolds compared to O2 plasma treatment, promoting cell proliferation and differentiation of hDPSCs.
Article
Materials Science, Biomaterials
Gen Li, Yubao Li, Xianhui Zhang, Pengfei Gao, Xue Xia, Shiqi Xiao, Jing Wen, Tao Guo, Weihu Yang, Jidong Li
Summary: This study successfully developed 3D printed scaffolds with strong mechanical strength and controllable pore structure, which can sustainably release strontium ions and simvastatin. These scaffolds significantly promote cell proliferation, angiogenesis, and functional bone regeneration by upregulating osteogenic and vasculogenic-related genes.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Article
Medicine, Research & Experimental
E. A. Akoulina, I. V. Demianova, I. I. Zharkova, V. V. Voinova, V. A. Zhuikov, D. D. Khaydapova, D. V. Chesnokova, K. A. Menshikh, A. A. Dudun, T. K. Makhina, G. A. Bonartseva, A. V. Volkov, T. F. Asfarov, S. Y. Ivanov, K. V. Shaitan, A. P. Bonartsev
Summary: The study showed that porous composite scaffolds based on PHB loaded with simvastatin have an impact on the growth and differentiation of mesenchymal stem cells. Simvastatin loading suppressed cell growth and increased alkaline phosphatase activity, indicating their osteoinductive properties.
BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE
(2021)
Letter
Biochemistry & Molecular Biology
Marianna Chierchia, Salvatore Chirumbolo, Luigi Valdenassi, Marianno Franzini
Summary: The aim of this paper was to create porous 3D substrates and scaffolds of polycaprolactone (PCL) and analyze the effect of ozone treatment on their performance. The tests showed that ozone treatment made the substrates softer and did not significantly alter the modulus and maximum effort values. Preliminary biological analysis indicated that ozone treatment improved aspects related to cell viability/proliferation.
CHEMICO-BIOLOGICAL INTERACTIONS
(2023)
Article
Chemistry, Physical
Junyu Sun, Xinxin Liu, Zongrui Chen, Lin Jiang, Mingwei Yuan, Minglong Yuan
Summary: In this study, a layered polycaprolactone (PCL) scaffold coated with PCEC was developed for the proliferation of human embryonic kidney cells and amplification of the Ad5 vector. The results showed that PCEC improved the hydrophilicity and cell culture ability of the PCL scaffold, resulting in a higher cell proliferation ratio. This work provides an effective method for the in-depth study of adenoviruses.
Article
Polymer Science
Evangelos Daskalakis, Mohamed H. Hassan, Abdalla M. Omar, Anil A. Acar, Ali Fallah, Glen Cooper, Andrew Weightman, Gordon Blunn, Bahattin Koc, Paulo Bartolo
Summary: This research investigates the accelerated hydrolytic degradation process of anatomically designed bone scaffolds with a pore size gradient and a rectangular shape. The effect of material composition is investigated using poly-epsilon-caprolactone (PCL) as the main scaffold material, reinforced with ceramics such as hydroxyapatite (HA), beta-tricalcium phosphate (TCP), and bioglass. The results show that the PCL/bioglass bone brick scaffolds exhibited faster degradation kinetics compared to other materials, and the degradation kinetics of rectangular scaffolds increased with the pore size. The degradation process was found to be faster on the external regions of the bone brick scaffolds, and the mechanical properties decreased during the degradation process.
Article
Engineering, Biomedical
Si-Yao Zheng, Zhi-Wei Liu, Hong-Lei Kang, Fan Liu, Guo-Ping Yan, Feng Li
Summary: Three-dimensional (3D)-printed scaffolds made of biodegradable polymers have been found to be effective in bone repair and regeneration, with low cytotoxicity, good biocompatibility, and the ability to enhance osteoblast proliferation.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2023)
Article
Biochemistry & Molecular Biology
Gianni Pecorini, Simona Braccini, Gianluca Parrini, Federica Chiellini, Dario Puppi
Summary: Polyhydroxyalkanoates are biopolyesters that can be used as unique scaffolding polymer candidates for tissue engineering due to their biocompatibility, biodegradability, environmental sustainability, processing versatility, and mechanical properties. This study explored the fabrication of tissue engineering scaffolds using a solution-extrusion additive manufacturing technique, called Computer-Aided Wet-Spinning (CAWS), by blending poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and poly(D,L-lactide-co-glycolide) (PLGA). The results showed that the blend composition influenced the morphological, physicochemical, and biological properties of the scaffolds, with increasing PLGA content leading to larger pore size, enhanced wettability, and improved thermal stability. In vitro experiments demonstrated the suitability of the scaffolds for cell colonization and differentiation towards an osteoblastic phenotype, particularly with scaffolds richer in PLGA.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Fatih Erci, Fatma Bayram Sariipek
Summary: In this study, poly(3-hydroxybutyrate)/poly(epsilon-cap-rolactone) (PHB/PCL) nanofibrous mats loaded with curcumin-beta cyclodextrin (CUR-CD) inclusion complex were successfully developed and characterized. The addition of curcumin and curcumin-beta cyclodextrin improved the thermal stability and antibacterial activity of the nanofibers. The fabricated PHB/PCL/CUR-CD nanofibrous mats showed potential as wound dressings.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2023)
Article
Polymer Science
Wasana Kosorn, Patcharaporn Wutticharoenmongkol
Summary: The scaffolds of PCL/PHBV blends fabricated from fused deposition modeling showed no cytotoxicity, with the surface-treated scaffold with a 25/75 PCL/PHBV blend ratio exhibiting the highest proliferation of porcine chondrocytes and total GAGs. The scaffolds with LP and 1 M NaOH surface treatment had the highest proliferation of cells and GAGs after culture. Degradation behaviors, as well as compressive strengths, were also studied, with results suggesting that scaffolds with LP and surface treatment had the highest potential for cartilage scaffold applications.
INTERNATIONAL JOURNAL OF POLYMER SCIENCE
(2021)
Article
Polymer Science
Carmen R. Tubio, Xabier Valle, Estela Carvalho, Joana Moreira, Pedro Costa, Daniela M. Correia, Senentxu Lanceros-Mendez
Summary: Poly(hydroxybutyrate-co-hidroxyvalerate) (PHBV) is a biodegradable polymer that can be used as a substitute for plastics made from fossil resources. By blending PHBV with poly(caprolactone) (PCL) and poly(lactic acid) (PLA), its properties can be improved, including morphology, wetting, structure, thermal, mechanical, and electrical characteristics. The addition of PCL and PLA also enhances the antibacterial activity of PHBV. This study demonstrates the potential of developing PHBV blends with PCL or PLA for specific applications.
Article
Materials Science, Textiles
Ramazan Erdem, Emre Yavuz, Esin Akarsu, Murat Akarsu, Ozlem Erdem Yilmaz, Ahmet Cosgun
Summary: In this study, various types of electrospun scaffolds were prepared and characterized. The results showed that the PLCL/COL/Ag-HAp scaffold had the smallest fiber diameter and the highest tensile strength. The incorporation of collagen and HAp also improved the hydrophilicity of the electrospun scaffolds. Additionally, the PLCL/COL/Ag-HAp scaffold exhibited remarkable antibacterial efficiency.
JOURNAL OF THE TEXTILE INSTITUTE
(2022)
Article
Polymer Science
Po-Kai Juan, Fang-Yu Fan, Wei-Chun Lin, Pei-Bang Liao, Chiung-Fang Huang, Yung-Kang Shen, Muhammad Ruslin, Chen-Han Lee
Summary: This study utilized PCL as an additive, nHA and β-TCP as ceramic powders, and NaCl and (NH4)HCO3 as porogens to fabricate 3D porous scaffolds with excellent mechanical properties and biocompatibility. Results showed that nHA and β-TCP could improve the mechanical properties, enhance the hydrophilic properties, and accelerate the degradation rate of the scaffolds.
Article
Neurosciences
Wan-Kyu Ko, Seong Jun Kim, Min-Jae Jo, Hyemin Choi, Donghyun Lee, Il Keun Kwon, Soo-Hong Lee, In-Bo Han, Seil Sohn
MOLECULAR NEUROBIOLOGY
(2019)
Article
Materials Science, Biomaterials
Yun Hee Youn, Sang Jin Lee, Go Ro Choi, Hak Rae Lee, Donghyun Lee, Dong Nyong Heo, Byung-Soo Kim, Jae Beum Bang, Yu-Shik Hwang, Vitor M. Correlo, Rui L. Reis, Sung Gap Im, Il Keun Kwon
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2019)
Article
Biochemistry & Molecular Biology
Dong Nyoung Heo, Han-Jun Kim, Donghyun Lee, Hyosung Kim, Sang Jin Lee, Hye-Rim Lee, Il Keun Kwon, Sun Hee Do
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2020)
Review
Biochemistry & Molecular Biology
Daniel A. Balikov, Emma H. Neal, Ethan S. Lippmann
TRENDS IN MOLECULAR MEDICINE
(2020)
Review
Biochemistry & Molecular Biology
Yeon-Hee Lee, Hee-Kyung Park, Q-Schick Auh, Haram Nah, Jae Seo Lee, Ho-Jin Moon, Dong Nyoung Heo, In San Kim, Il Keun Kwon
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Nanoscience & Nanotechnology
Wan-Kyu Ko, Seong Jun Kim, Dong Nyoung Heo, In-Bo Han, Sewha Kim, Il Keun Kwon, Seil Sohn
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
(2020)
Article
Chemistry, Multidisciplinary
Na Re Ko, Se Young Van, Sung Hwa Hong, Seog-Young Kim, Miran Kim, Jae Seo Lee, Sang Ju Lee, Yong-kyu Lee, Il Keun Kwon, Seung Jun Oh
Article
Engineering, Biomedical
Timothy C. Boire, Lauren E. Himmel, Fang Yu, Christy M. Guth, Bryan R. Dollinger, Thomas A. Werfel, Daniel A. Balikov, Craig L. Duvall
Summary: Neointimal hyperplasia is a common cause of failure in arteriovenous fistulas and vascular grafts. Perivascular wraps made of biodegradable materials have shown promise in reducing neointimal hyperplasia by promoting neovascularization. This study investigated the impact of pore size and spacing on implant neovascularization, finding that wider-spaced, smaller-sized pores induced a more robust angiogenic response.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2021)
Article
Cell & Tissue Engineering
Jonah C. Rosch, Emma H. Neal, Daniel A. Balikov, Mohsin Rahim, Ethan S. Lippmann
CELLULAR AND MOLECULAR BIOENGINEERING
(2020)
Review
Biochemistry & Molecular Biology
Brian W. Basinski, Daniel A. Balikov, Michael Aksu, Qiang Li, Rajesh C. Rao
Summary: This article reviews the new functions of chromatin modifiers in retinal development and discusses their role in ocular congenital malformations, emphasizing the insights provided by advances in epigenomic technologies for visual disorders. Moreover, it highlights the potential of combining epigenetics knowledge with pluripotent stem cells for modeling congenital retinal diseases and cell therapy.
TRENDS IN MOLECULAR MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Andi K. Cani, Marcus A. Toral, Daniel A. Balikov, Bryan L. Betz, Kevin Hu, Chia-Jen Liu, Matthew V. Prifti, Arul M. Chinnaiyan, Scott A. Tomlins, Vinit B. Mahajan, Rajesh C. Rao
Summary: This study presents the molecular diagnostic workup of a rare case of bilateral T cell VRL, identifying potentially targetable alterations in the genomic landscape. The use of next-generation sequencing of vitreous-derived DNA revealed copy number gain of BRAF and copy number loss of tumor suppressor DNMT3A. This study marks the first exploration of the T cell VRL cancer genome and provides support for using vitreous liquid biopsy in precision oncology treatments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Daniel A. Balikov, Kevin Hu, Chia-Jen Liu, Bryan L. Betz, Arul M. Chinnaiyan, Laxmi V. Devisetty, Sriram Venneti, Scott A. Tomlins, Andi K. Cani, Rajesh C. Rao
Summary: In this study, targeted DNA next-generation sequencing was utilized to analyze brain and vitreous samples from two patients with VRL and PCNSL, revealing a clonal relationship between eye and brain samples while also showing heterogeneity in subclonal alterations.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Genetics & Heredity
Daniel A. Balikov, Adam Jacobson, Lev Prasov
Summary: Monogenic syndromic disorders often present with ocular manifestations such as glaucoma, which may go undetected in children and serve as the initial sign of a systemic syndrome. Understanding syndromes associated with glaucoma is crucial for medical geneticists and ophthalmologists.
Article
Computer Science, Interdisciplinary Applications
Jonah C. Rosch, Daniel A. Balikov, Franklin Gong, Ethan S. Lippmann
ENGINEERING REPORTS
(2020)
Article
Chemistry, Multidisciplinary
Sang Jin Lee, Ji Suk Choi, Min Rye Eom, Ha Hyeon Jo, Il Keun Kwon, Seong Keun Kwon, Su A. Park
