Article
Virology
Fengmei Yang, Yanyan Li, Yongjie Li, Weihua Jin, Suqin Duan, Hongjie Xu, Yuan Zhao, Zhanlong He, Yasushi Ami, Yuriko Suzaki, Yen Hai Doan, Naokazu Takeda, Wenjing Zhang, Masamichi Muramatsu, Tian-Cheng Li
Summary: Through experimental studies, it was found that cynomolgus and rhesus monkeys are susceptible to rat hepatitis E virus (rat HEV) and may cause zoonotic infections. These findings provide an animal model for the development of vaccines.
Article
Immunology
Mark Chernyshev, Mateusz Kaduk, Martin Corcoran, Gunilla B. Karlsson Hedestam
Summary: This study quantified VDJ gene usage levels in the unmutated IgM repertoires of 45 macaques, revealing similar patterns of gene usage among macaque subgroups and individuals. Unlike humans, macaques showed a higher usage of IGHV4 family genes and evidence of evolutionary expansion of this gene family.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Virology
Yishu Gong, Matilda Mostrom, Claire Otero, Sarah Valencia, Alice F. Tarantal, Amitinder Kaur, Sallie R. Permar, Cliburn Chan
Summary: Approximately 0.7% of infants are born with congenital cytomegalovirus (CMV), with potential long-term consequences. The rhesus CMV (RhCMV) infection of rhesus monkey fetuses is an important animal model for studying CMV infection. A mathematical model was developed to study the risk of placental transmission of CMV, considering factors such as timing of inoculation, maternal immune suppression, and hyper-immune globulin infusion.
Article
Immunology
Courtney Woolsey, Alyssa C. Fears, Viktoriya Borisevich, Krystle N. Agans, Natalie S. Dobias, Abhishek N. Prasad, Daniel J. Deer, Joan B. Geisbert, Karla A. Fenton, Thomas W. Geisbert, Robert W. Cross
Summary: Ebola virus disease (EVD) continues to be a serious threat to global health due to its high mortality rate and re-emergence. In this study, rhesus and cynomolgus macaques were used as models to investigate the infection of Sudan ebolavirus (SUDV). The results showed that cynomolgus macaques had a 100% mortality rate, while one rhesus macaque survived to the end of the study. The infected macaques exhibited similar characteristics to human EVD.
EMERGING MICROBES & INFECTIONS
(2022)
Article
Multidisciplinary Sciences
Francisco J. Salguero, Andrew D. White, Gillian S. Slack, Susan A. Fotheringham, Kevin R. Bewley, Karen E. Gooch, Stephanie Longet, Holly E. Humphries, Robert J. Watson, Laura Hunter, Kathryn A. Ryan, Yper Hall, Laura Sibley, Charlotte Sarfas, Lauren Allen, Marilyn Aram, Emily Brunt, Phillip Brown, Karen R. Buttigieg, Breeze E. Cavell, Rebecca Cobb, Naomi S. Coombes, Alistair Darby, Owen Daykin-Pont, Michael J. Elmore, Isabel Garcia-Dorival, Konstantinos Gkolfinos, Kerry J. Godwin, Jade Gouriet, Rachel Halkerston, Debbie J. Harris, Thomas Hender, Catherine M. K. Ho, Chelsea L. Kennard, Daniel Knott, Stephanie Leung, Vanessa Lucas, Adam Mabbutt, Alexandra L. Morrison, Charlotte Nelson, Didier Ngabo, Jemma Paterson, Elizabeth J. Penn, Steve Pullan, Irene Taylor, Tom Tipton, Stephen Thomas, Julia A. Tree, Carrie Turner, Edith Vamos, Nadina Wand, Nathan R. Wiblin, Sue Charlton, Xiaofeng Dong, Bassam Hallis, Geoffrey Pearson, Emma L. Rayner, Andrew G. Nicholson, Simon G. Funnell, Julian A. Hiscox, Mike J. Dennis, Fergus Gleeson, Sally Sharpe, Miles W. Carroll
Summary: This study demonstrates that SARS-CoV-2 replicates in the upper and lower respiratory tract of both rhesus and cynomolgus macaques, causing pulmonary lesions. Immune responses against SARS-CoV-2 are similar in both species, suggesting that both macaque species authentically represent mild to moderate forms of COVID-19. The findings provide valuable insights for evaluating interventions against SARS-CoV-2.
NATURE COMMUNICATIONS
(2021)
Article
Immunology
Amanda N. Pinski, Kevin J. Maroney, Andrea Marzi, Ilhem Messaoudi
Summary: Cynomolgus and rhesus macaques show different clinical presentations after Ebola virus infection, with differential transcriptional responses likely mediating these distinct outcomes.
EMERGING MICROBES & INFECTIONS
(2021)
Article
Biochemistry & Molecular Biology
Xue-Hui Wang, Tian-Zhang Song, Hong-Yi Zheng, Yi-Hui Li, Yong-Tang Zheng
Summary: The destruction of intestinal epithelial barrier appears earlier than mucosal immune dysfunction in early stages of HIV and SIV infections, primarily due to oxidative stress-induced DNA damage and apoptosis. Mitochondrial dysfunction and oxidative stress are identified as key contributors to gut epithelial barrier disruption in early SIV-infected rhesus macaques.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Virology
Yujuan Yue, W. L. William Chang, Julia Li, Nancy Nguyen, Kimberli A. Schmidt, Philip R. Dormitzer, Xinzhen Yang, Peter A. Barry
Summary: The study establishes an oral RhCMV infection model that mimics natural HCMV infection and provides valuable insights into the virological and immunological parameters of the infection. This research has significant implications for the design of HCMV vaccines for human immunization.
JOURNAL OF VIROLOGY
(2022)
Article
Virology
Dylan M. Johnson, Trevor Brasel, Shane Massey, Jeanon Smith, Shannon Wallace, Xiaoying Yu, David W. Beasley, Jason E. Comer
Summary: This study is the first to examine the Kikwit strain of EBOV in the macaque model and detect the virus in the vitreous fluid, which has been proposed as a viral reservoir. The macaque challenge model described here faithfully recapitulates the prodrome of human EVD, paving the way for advanced studies on contact transmission and persistence of the virus.
JOURNAL OF VIROLOGY
(2023)
Article
Virology
Mable Chan, Meenakshi Tiwary, Helen L. Wu, Nikesh Tailor, Robert Vendramelli, Jonathan Audet, Bryce M. Warner, Kevin Tierney, Alix Albietz, Thang Truong, Kaylie Doan, Alexander Bello, Marnie Willman, Bryan D. Griffin, Patrick W. Hanley, Jamie Lovaglio, David Safronetz, Jim Strong, Jonah B. Sacha, Darwyn Kobasa
Summary: The world remains at risk of an influenza pandemic, and the development of new therapeutic and preventative modalities is critically important. While animal models, particularly nonhuman primates like macaques, are central to the development of new therapies and vaccine approaches, rhesus and cynomolgus macaques may not be ideal for testing novel pandemic influenza-specific vaccines and therapies, indicating the need for other physiologically relevant nonhuman primate models.
JOURNAL OF VIROLOGY
(2022)
Article
Immunology
Suthirote Meesawat, Saradee Warit, Yuzuru Hamada, Suchinda Malaivijitnond
Summary: We identified tuberculosis in 1,836 macaques from various macaque populations in Thailand. Throat swab specimens were found to be the best sample medium for detecting Mycobacterium tuberculosis complex (MTBC) infections. The prevalence of MTBC was higher in rhesus macaques compared to the common and Burmese long-tailed macaques, and a higher percentage of adults were infected compared to subadults and juveniles. Bidirectional zoonotic transmission should be considered a possible public health concern.
EMERGING INFECTIOUS DISEASES
(2023)
Article
Immunology
Christina M. Newman, Alice F. Tarantal, Michele L. Martinez, Heather A. Simmons, Terry K. Morgan, Xiankun Zeng, Jenna R. Rosinski, Mason I. Bliss, Ellie K. Bohm, Dawn M. Dudley, Matthew T. Aliota, Thomas C. Friedrich, Christopher J. Miller, David H. O'Connor
Summary: The study conducted on three rhesus macaques challenged with ZIKV through sexual transmission indicates that sexual transmission of ZIKV may represent an underappreciated risk of pregnancy loss during early gestation.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Dawn M. Dudley, Michelle R. Koenig, Laurel M. Stewart, Matthew R. Semler, Christina M. Newman, Phoenix M. Shepherd, Keisuke Yamamoto, Meghan E. Breitbach, Michele Schotzko, Sarah Kohn, Kathleen M. Antony, Hongyu Qiu, Priyadarshini Tunga, Deborah M. Anderson, Wendi Guo, Maria Dennis, Tulika Singh, Sierra Rybarczyk, Andrea M. Weiler, Elaina Razo, Ann Mitzey, Xiankun Zeng, Jens C. Eickhoff, Emma L. Mohr, Heather A. Simmons, Michael K. Fritsch, Andres Mejia, Matthew T. Aliota, Thomas C. Friedrich, Thaddeus G. Golos, Shantha Kodihalli, Sallie R. Permar, David H. O'Connor
Summary: Currently, there are no approved drugs to treat Zika virus infection during pregnancy. However, administering ZIKV-specific human immune globulin has shown promise in controlling the infection and preventing congenital Zika syndrome (CZS) by generating antibodies. Further studies are needed to fully assess the impact of this treatment.
Article
Medicine, Research & Experimental
Yongjun Sui, Jianping Li, Roushu Zhang, Sunaina Kiran Prabhu, Hanne Andersen, David Venzon, Anthony Cook, Renita Brown, Elyse Teow, Jason Velasco, Jack Greenhouse, Tammy Putman-Taylor, Tracey-Ann Campbell, Laurent Pessaint, Ian N. Moore, Laurel Lagenaur, Jim Talton, Matthew W. Breed, Josh Kramer, Kevin W. Bock, Mahnaz Minai, Bianca M. Nagata, Mark G. Lewis, Lai-Xi Wang, Jay A. Berzofsky
Summary: Comparing two adjuvanted subunit vaccines in rhesus macaques, the study found that both vaccines were effective in protecting against respiratory SARS-CoV-2 exposure, despite potential differences in mucosal and systemic protective mechanisms. The mucosal vaccine was shown to be safe after multiple doses, efficiently clearing the input virus from the nasal cavity, and may serve as a potent complementary boost to conventional systemic vaccines for overall better protection.
Article
Biochemistry & Molecular Biology
Jing Xue, Huihui Chong, Yuanmei Zhu, Jingjing Zhang, Ling Tong, Jiahan Lu, Ting Chen, Zhe Cong, Qiang Wei, Yuxian He
Summary: The new HIV fusion-inhibitory lipopeptide LP-98 showed potent antiviral activity in rhesus macaques, with some achieving potential posttreatment control effectiveness.
Article
Immunology
Bridget S. Fisher, Katherine A. Fancher, Andrew T. Gustin, Cole Fisher, Matthew P. Wood, Michael Gale, Benjamin J. Burwitz, Jeremy Smedley, Nichole R. Klatt, Nina Derby, Donald L. Sodora
Summary: Liver disease is a major cause of illness and death in HIV-infected individuals. This study found that gut dysbiosis and microbial translocation can lead to changes in the liver microbiome, even during successful antiretroviral therapy. Specifically, an increase in Mycobacterium bacteria was observed in both SIV-infected and SIV-treated macaques. Additionally, the activation of inflammatory responses in liver cells was associated with immune cell recruitment in SIV-infected and treated macaques.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Sreya Biswas, Lauren N. Rust, Jochen M. Wettengel, Sofiya Yusova, Miranda Fischer, Julien N. Carson, Josie Johnson, Lei Wei, Trason Thode, Mohan R. Kaadige, Sunil Sharma, Majd Agbaria, Benjamin N. Bimber, Thomas Tu, Ulrike Protzer, Alexander Ploss, Jeremy V. Smedley, Gershon Golomb, Jonah B. Sacha, Benjamin J. Burwitz
Summary: In this study, a second-generation model of HBV infection in rhesus macaques is presented and characterized. The study shows that extended infection in macaques requires suppression of host immunity. The findings provide important insights into understanding the mechanisms of HBV infection and may potentially accelerate the development of new curative therapies for HBV.
NATURE COMMUNICATIONS
(2022)
Article
Medicine, Research & Experimental
Junghyun Ryu, William Chan, Jochen M. Wettengel, Carol B. Hanna, Benjamin J. Burwitz, Jon D. Hennebold, Benjamin N. Bimber
Summary: In this study, the authors present a method called esTag-PCR for mapping transgene integration and copy number. They demonstrate that esTag-PCR has higher accuracy and efficiency compared to alternative methods and can be used to validate and screen edited embryos before implantation. This technique represents an important advancement in the field of genome engineering.
MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT
(2022)
Article
Multidisciplinary Sciences
Junghyun Ryu, John P. Statz, William Chan, Fernanda C. Burch, John V. Brigande, Beth Kempton, Edward V. Porsov, Lauren Renner, Trevor McGill, Benjamin J. Burwitz, Carol B. Hanna, Martha Neuringer, Jon D. Hennebold
Summary: In this study, a nonhuman primate model of Usher syndrome type 1B (USH1B) was created using CRISPR/Cas9 technology. The efficiency of different CRISPR components was compared, and the desired mutations were confirmed by preimplantation genetic testing (PGT). This study demonstrates the feasibility of using CRISPR technologies to create NHP models of human diseases.
SCIENTIFIC REPORTS
(2022)
Article
Microbiology
Daniel Malouli, Roxanne M. Gilbride, Helen L. Wu, Joseph M. Hwang, Nicholas Maier, Colette M. Hughes, Daniel Newhouse, David Morrow, Abigail B. Ventura, Lynn Law, Jennifer Tisoncik-Go, Leanne Whitmore, Elise Smith, Inah Golez, Jean Chang, Jason S. Reed, Courtney Waytashek, Whitney Weber, Husam Taher, Luke S. Uebelhoer, Jennie L. Womack, Matthew R. McArdle, Junwei Gao, Courtney R. Papen, Jeffrey D. Lifson, Benjamin J. Burwitz, Michael K. Axthelm, Jeremy Smedley, Klaus Fruh, Michael Jr Jr Gale, Louis J. Picker, Scott G. Hansen, Jonah B. Sacha
Summary: This study demonstrates that activating MHC-E-restricted CD8+ T cells requires matching cytomegalovirus (CMV) to its host species, using different species of cynomolgus macaques CMV vectors expressing SIV antigens. Vaccinated cynomolgus macaques with species-matched CMV vectors mounted MHC-E-restricted CD8+ T cells and half of them controlled SIV replication strictly post-challenge. The protected animals showed a vaccine-induced IL-15 transcriptomic signature associated with efficacy in rhesus macaques.
CELL HOST & MICROBE
(2022)
Article
Cell & Tissue Engineering
Suhas Sureshchandra, Chi N. Chan, Jacob J. Robino, Lindsay K. Parmelee, Michael J. Nash, Stephanie R. Wesolowski, Eric M. Pietras, Jacob E. Friedman, Diana Takahashi, Weining Shen, Xiwen Jiang, Jon D. Hennebold, Devorah Goldman, William Packwood, Jonathan R. Lindner, Charles T. Roberts, Benjamin J. Burwitz, Ilhem Messaoudi, Oleg Varlamov
Summary: Maternal obesity affects fetal hematopoiesis, leading to inflammatory responses in fetal bone marrow cells and decreased B cell development. Maternal high-fat diet also impairs the engraftment of fetal hematopoietic stem and progenitor cells in immunodeficient mice.
Article
Biochemistry & Molecular Biology
Jochen M. Wettengel, Lea Hansen-Palmus, Sofiya Yusova, Lauren Rust, Sreya Biswas, Julien Carson, Junghyun Ryu, Benjamin N. Bimber, Jon D. Hennebold, Benjamin J. Burwitz
Summary: CRISPR/Cas systems are promising tools for genome editing. A novel reporter system called BETLE is described, which enables fast, sensitive, and cell-specific detection of genome editing. BETLE includes multiple reporter proteins encoded in different open-reading frames, allowing for the analysis of editing and localization of genome-edited cells. It is a versatile tool that can be used for in vitro evaluation and optimization of CRISPR/Cas genome editing and HDR.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Meeting Abstract
Immunology
Nina R. Derby, Katherine A. Fancher, Sreya Biswas, Sofiya Yusova, Cristina Luevano-Santos, Jeremy Smedley, Cristina Pacheco, Benjamin Burwitz, Donald L. Sodora
JOURNAL OF IMMUNOLOGY
(2022)
Meeting Abstract
Gastroenterology & Hepatology
Lauren Rust, Michael J. Ricciardi, Nuria Pedreno-Lopez, Sofiya Yusova, Sreya Biswas, Miranda Fischer, Gabriela M. Webb, Lucas Gonzalez-Nieto, Thomas B. Voigt, Johan J. Louw, Fernanda D. Laurino, John R. DiBello, Hans-Peter Raue, Lidiane M. S. Raphael, Aaron Yrizarry-Medina, Brandon C. Rosen, Rebecca Agnor, Lina Gao, Caralyn Labriola, Michael Axthelm, Jeremy Smedley, Justin G. Julander, Myrna C. Bonaldo, Laura M. Walker, Ilhem Messaoudi, Mark K. Slifka, Dennis R. Burton, Esper G. Kallas, Jonah B. Sacha, David I. Watkins, Benjamin J. Burwitz
Meeting Abstract
Gastroenterology & Hepatology
Sreya Biswas, Lauren Rust, Sofiya Yusova, Jochen M. Wettengel, Junghyun Ryu, Benjamin N. Bimber, Carol Hanna, Jon D. Hennebold, Benjamin J. Burwitz
Meeting Abstract
Ophthalmology
Martha Neuringer, Junghyun Ryu, Jon D. Hennebold, John P. Statz, William Chan, Cathy Ramsey, Fernanda Burch, Beth Kempton, Edward V. Porsov, Lauren Renner, Benjamin Burwitz, Carol B. Hannah, John V. Brigande
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2022)
Meeting Abstract
Veterinary Sciences
Helen Wu, Whitney Weber, Courtney Waytashek, Carla Boyle, Shaheed Abdulhaqq, Christine Shriver-Munsch, Tonya Swanson, Mina Northrup, Kimberly Armantrout, Heidi Price, Mitchell Robertson-LeVay, Jason Reed, Katherine Bateman, Stephanie Junell, Mitthra Kumar, Emily Fray, Benjamin Bimber, Rhonda MacAllister, Alfred Legasse, Robert Siliciano, Janet Siliciano, Michael Axthelm, Cassandra Moats, Jeremy Smedley, Theodore Hobbs, Lauren Martin, Gabrielle Meyers, Richard Maziarz, Benjamin Burwitz, Jeffrey Stanton, Jonah Sacha
JOURNAL OF MEDICAL PRIMATOLOGY
(2022)
Meeting Abstract
Veterinary Sciences
Sreya Biswas, Lauren Rust, Jochen Wettengel, Sofiya Yusova, Miranda Fischer, Julien Carson, Jeremy Smedley, Benjamin Burwitz
JOURNAL OF MEDICAL PRIMATOLOGY
(2022)
Meeting Abstract
Veterinary Sciences
Nina Derby, Katerine Fancher, Sreya Biswas, Sofiya Yusova, Cole Fisher, Jeremy Smedley, M. Pacheco, Benjamin Burwitz, Donald Sodora
JOURNAL OF MEDICAL PRIMATOLOGY
(2022)