Article
Parasitology
Xuexing Zhang, Haichao Wei, Yangminghui Zhang, Yan Zhao, Lin Wang, Yubing Hu, Wang Nguitragool, Jetsumon Sattabongkot, John Adams, Liwang Cui, Yaming Cao, Qinghui Wang
Summary: This study analyzed the genetic diversity of the PvRBP2b gene in global P. vivax populations and found that it was distributed unevenly, with highly conserved critical residues and significant geographic divergence. These results are important for the development of PvRBP2b-based vaccines.
PARASITES & VECTORS
(2022)
Article
Infectious Diseases
Siqi Wang, Peng Tian, Shigang Li, Hui Liu, Xiangrui Guo, Fang Huang
Summary: This study analyzed the genetic diversity of Pvs25 and Pvs28 genes in Plasmodium vivax by collecting samples from patients in China. The results showed that the genetic diversity of these two genes was relatively limited in China. These findings provide valuable information for the design and optimization of transmission-blocking vaccines.
BMC INFECTIOUS DISEASES
(2022)
Article
Multidisciplinary Sciences
Martha A. Clark, Usheer Kanjee, Gabriel W. Rangel, Laura Chery, Anjali Mascarenhas, Edwin Gomes, Pradipsinh K. Rathod, Carlo Brugnara, Marcelo U. Ferreira, Manoj T. Duraisingh
Summary: During Plasmodium intra-erythrocytic development, parasites compromise the structural integrity of host red-blood cells. Researchers found that P. vivax infection destabilizes host reticulocytes, which are less stable than normocytes infected with P. falciparum.
NATURE COMMUNICATIONS
(2021)
Article
Microbiology
Haung Naw, Jung-Mi Kang, Mya Moe, Jinyoung Lee, Huong Giang Le, Tuan Cuong Vo, Yi Yi Mya, Moe Kyaw Myint, Zaw Than Htun, Tong-Soo Kim, Ho-Joon Shin, Byoung-Kuk Na
Summary: Despite a significant decline in malaria incidence in Myanmar, malaria remains a major public health concern. Recent years have seen an increase in genetic diversity of Plasmodium vivax population, attributed to accumulated mutations and recombination. Continuous molecular surveillance of genetic variation in Myanmar's P. vivax is necessary to monitor the ongoing genetic expansion.
Article
Pharmacology & Pharmacy
Rajeev K. Mehlotra, Andrea Gaedigk, Rosalind E. Howes, Tovonahary A. Rakotomanga, Arsene C. Ratsimbasoa, Peter A. Zimmerman
Summary: The study found genetic variation in CYP2D6 in the population of Madagascar, where some individuals are predicted to be intermediate metabolizers, which is associated with therapeutic failure of PQ. The results also provide insights into the unique Asian-African admixed origin of Malagasy populations.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Immunology
Md Atique Ahmed, Gauspasha Yusuf Deshmukh, Rehan Haider Zaidi, Ahmed Saif, Mohammed Abdulrahman Alshahrani, Syeda Wasfeea Wazid, Saurav Jyoti Patgiri, Fu-Shi Quan
Summary: The study discovered the potential of high molecular weight rhoptry protein 2 (RhopH2) in producing cross-protective immunity and suggested it as an excellent target for malaria vaccine development. Further research on genetic diversity and antibody functionality specific to the novel conserved epitope is needed for protection against infection.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Parasitology
Xin Zhao, Yubing Hu, Yan Zhao, Lin Wang, Zifang Wu, Myat Thu Soe, Myat Phone Kyaw, Liwang Cui, Xiaotong Zhu, Yaming Cao
Summary: Low genetic diversity of Pvs230 was observed in Plasmodium vivax populations from the China-Myanmar border and central Myanmar. Geographically specific haplotypes were identified. Some mutations were located within potential B-cell epitopes.
PARASITES & VECTORS
(2022)
Article
Infectious Diseases
Andres Nunez, Francis B. Ntumngia, Yasel Guerra, John H. Adams, Fabian E. Saenz
Summary: This study investigated the diversity of invasion ligands in Plasmodium vivax isolates from Ecuador and found that the genetic diversity of the invasion genes pvdbpII and pvama1 was similar to that found in other malaria endemic countries. The highest genetic diversity was observed in the Amazonian region of Ecuador.
Article
Microbiology
Tian-Qi Shi, Hai-Mo Shen, Shen-Bo Chen, Kokouvi Kassegne, Yan-Bing Cui, Bin Xu, Jun-Hu Chen, Bin Zheng, Yue Wang
Summary: Although China was certified malaria-free in 2021, the presence of malaria in border areas and importation of cases remain challenges. Research on genetic diversity and natural selection of PvDBP-II from P. vivax isolates in China-Myanmar border area revealed significant characteristics in this region.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Parasitology
Jieyun Ge, Qiubo Wang, Gangcheng Chen, Kokouvi Kassegne, Hangye Zhang, Jiali Yu, Jianxia Tang, Bo Wang, Feng Lu, Jun Cao, Eun-Taek Han, Yang Cheng
Summary: The C-terminal of PvRAMA exhibits high conservation and immunogenicity, inducing broadly reactive humoral and cellular immune responses. These findings support the potential of PvRAMA as a vaccine candidate against P. vivax infection.
PARASITES & VECTORS
(2022)
Article
Parasitology
Byoung-Kuk Na, Tong-Soo Kim, Khin Lin, Moon-Chang Baek, Dong-Il Chung, Yeonchul Hong, Youn-Kyoung Goo
Summary: The study evaluated genetic polymorphisms and phylogenetic relationships of four vir genes in Myanmar P. vivax population, finding that vir 12 was the most genetically diverse while vir 4 was the most conserved. Haplotypes of vir 4 and 12 from Myanmar clustered with those from India and Republic of Korea, confirming high genetic polymorphism of vir genes. These results provide valuable information for further functional research and practical use of vir genes.
PARASITOLOGY INTERNATIONAL
(2021)
Article
Immunology
Colleen M. Leonard, Ashenafi Assefa, Heven Sime, Hussein Mohammed, Amha Kebede, Hiwot Solomon, Chris Drakeley, Matt Murphy, Jimee Hwang, Eric Rogier
Summary: Assessing the spatial distribution of malaria exposure using multiple metrics can improve understanding of malaria transmission dynamics in a region. In low-transmission areas, antibody data provide a useful marker to assess malaria exposure. This study conducted blood sample assessments in three regions in Ethiopia, showing low prevalence of malaria infection.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Immunology
Yan-Bing Cui, Hai-Mo Shen, Shen-Bo Chen, Kokouvi Kassegne, Tian-Qi Shi, Bin Xu, Jun-Hu Chen, Jia-Hong Wu, Yue Wang
Summary: This study analyzed the genetic polymorphism and selection of the PvAMA-1 gene in Plasmodium vivax isolates from imported cases to China. The results showed high genetic diversity and positive selection in the PvAMA-1 gene, which are important for the design and development of malaria vaccines.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Infectious Diseases
Ibrar Ullah, Sahib Gul Afridi, Muhammad Israr, Hizbullah Khan, Sulaiman Shams, Komal Zaib, Huong Giang Le, Jung-Mi Kang, Byoung-Kuk Na, Asifullah Khan
Summary: The study revealed low genetic differentiation across the pvama-1 DI among the P. vivax isolates acquired from widespread regions of KP province of Pakistan. This information may implicate in future vaccine designing strategies based on antigenic features of pvama-1.
BMC INFECTIOUS DISEASES
(2022)
Article
Infectious Diseases
Zainab Bibi, Anam Fatima, Rehana Rani, Ayesha Maqbool, Samea Khan, Shumaila Naz, Shahid Waseem
Summary: The study focused on the genetic diversity of Plasmodium vivax in Pakistan by analyzing pvcsp and pvmsp-1 genes as molecular markers. The results showed high levels of polymorphism at the central-repeat region of pvcsp gene and block 2 of pvmsp-1 gene, indicating the need for further research on the genetic diversity of P. vivax in order to better understand its population dynamics.
Article
Infectious Diseases
Andargie Abate, Isabelle Bouyssou, Solenne Mabilotte, Cecile Doderer-Lang, Laurent Dembele, Didier Menard, Lemu Golassa
Summary: This study aimed to determine the distribution of P. vivax asexual parasitaemia according to Duffy antigen polymorphisms in Ethiopia. The results showed that P. vivax parasitaemia was significantly lower in Duffy-negative patients, suggesting a less efficient invasion of Duffy-negative reticulocytes by P. vivax. Additionally, the low parasitaemia observed in Duffy-negative individuals could serve as an undetected reservoir, potentially delaying the elimination of vivax malaria in Ethiopia.
Article
Cell Biology
Matthias Paulus Wagner, Pauline Formaglio, Olivier Gorgette, Jerzy Michal Dziekan, Christele Huon, Isabell Berneburg, Stefan Rahlfs, Jean-Christophe Barale, Sheldon I. Feinstein, Aron B. Fisher, Didier Menard, Zbynek Bozdech, Rogerio Amino, Lhousseine Touqui, Chetan E. Chitnis
Summary: Plasmodium falciparum imports a host antioxidant enzyme, PRDX6, during hemoglobin uptake, which repairs lipid peroxidation damage, but inhibition of PRDX6 leads to parasite death.
Review
Infectious Diseases
Dysoley Lek, Agus Rachmat, Dustin Harrison, Geoffrey Chin, Suwanna Chaoratanakawee, David Saunders, Didier Menard, William O. Rogers
Summary: This study evaluated the effectiveness of three therapies for uncomplicated falciparum malaria in Northern Cambodia. The results showed that DHA/PPQ and ATQ/PG had acceptable efficacy, while AS/MQ exhibited some level of resistance. The study also found significant evidence of artemisinin resistance in Northern Cambodia as early as 2009.
Review
Microbiology
Daniel A. Pfeffer, Ari Winasti Satyagraha, Arkasha Sadhewa, Mohammad Shafiul Alam, Germana Bancone, Yap Boum, Marcelo Brito, Liwang Cui, Zeshuai Deng, Gonzalo J. Domingo, Yongshu He, Wasif A. Khan, Mohammad Golam Kibria, Marcus Lacerda, Didier Menard, Wuelton Monteiro, Sampa Pal, Sunil Parikh, Arantxa Roca-Feltrer, Michelle Roh, Mahmoud M. Sirdah, Duoquan Wang, Qiuying Huang, Rosalind E. Howes, Ric N. Price, Benedikt Ley
Summary: This study investigates the variation in G6PD activity caused by different genetic variants and finds that different mutations can lead to varying degrees of enzyme deficiency. Some of these mutations are associated with clinically relevant enzymatic deficiencies.
Article
Multidisciplinary Sciences
Xinyu Yu, Changhong Wang, Yuemeng Zhao, Jianxia Tang, Meng Zhu, Lucien Platon, Richard Culleton, Guoding Zhu, Didier Menard, Qingfeng Zhang, Jun Cao
Summary: The emergence and spread of artemisinin-tolerant malaria parasites pose a global threat to malaria control programs. Mutations in the Kelch13 protein's propeller domain lead to Plasmodium falciparum artemisinin resistance (ART-R). The reduced susceptibility and extended ring stage in PfKelch13 mutant lines indicate altered energy and porphyrin metabolism, specifically in the tricarboxylic acid (TCA) cycle, glycolysis, and amino acid metabolism. These findings suggest that the adaptative metabolic plasticity, manifested through defined metabolic remodeling, serves as a trigger for ART-R.
Article
Microbiology
Lucien Platon, David A. Baker, Didier Menard
Summary: The ring-stage survival assay is the reference assay to measure in vitro Plasmodium falciparum artemisinin partial resistance. The main challenge of the standard protocol is to generate 0-to-3-h postinvasion ring stages (the stage least susceptible to artemisinin) from schizonts obtained by sorbitol treatment and Percoll gradient.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Infectious Diseases
Andargie Abate, Jifar Hassen, Laurent Dembele, Didier Menard, Lemu Golassa
Summary: This study conducted a membrane feeding assay on 44 P. vivax infected patients in Adama city, Ethiopia, and found that individuals with Duffy blood group had a higher infectiousness to Anopheles mosquitoes, but the difference was not statistically significant. This suggests that Duffy antigen polymorphisms may contribute to the transmissibility of P. vivax gametocytes to mosquitoes, but further studies are needed.
Article
Infectious Diseases
Mamadou Samb Yade, Baba Dieye, Romain Coppee, Aminata Mbaye, Mamadou Alpha Diallo, Khadim Diongue, Justine Bailly, Atikatou Mama, Awa Fall, Alphonse Birane Thiaw, Ibrahima Mbaye Ndiaye, Tolla Ndiaye, Amy Gaye, Abdoulaye Tine, Younouss Diedhiou, Amadou Mactar Mbaye, Cecile Doderer-Lang, Mamane Nassirou Garba, Amy Kristine Bei, Didier Menard, Daouda Ndiaye
Summary: This study investigated the susceptibility to artemisinin in Senegal in 2017 and found that artemisinin was still fully effective in the Thies region. The combination of ex vivo RSA and TADS methods is important for monitoring artemisinin resistance in Africa.
Article
Parasitology
Mariangela L. L'Episcopia, Cecile Doderer-Lang, Edvige Perrotti, Giovanni Battista Priuli, Serena Cavallari, Carlotta Guidetti, Francesco Bernieri, Didier Menard, Carlo Severini
Summary: Benin, like other countries in sub-Saharan Africa, is still heavily burdened by malaria. This study conducted molecular surveillance on malaria parasites in Benin and found evidence of antimalarial drug resistance.
Article
Infectious Diseases
Casimire Wendlamita Tarama, Harouna Sore, Mafama Siribie, Siaka Debe, Rene Kinda, Adama Ganou, Wendyam Gerard Nonkani, Farida Tiendrebeogo, Winnie Bantango, Kassoum Yira, Aladari Sagnon, Sonia Ilboudo, Esther Yeri Hien, Moussa Wandaogo Guelbeogo, NFale Sagnon, Yves Traore, Didier Menard, Adama Gansane
Summary: This study aimed to assess the proportions of mutations in the genes associated with drug resistance in Plasmodium falciparum isolates in Burkina Faso. The findings suggest that the efficacy of current antimalarial drugs is not significantly affected by highly resistant mutants.
Article
Infectious Diseases
Abnet Abebe, Didier Menard, Sisay Dugassa, Ashenafi Assefa, Jonathan J. Juliano, Eugenia Lo, Lemu Golassa
Summary: Both microscopy and RDT showed moderate agreement with PCR in the detection and identification of P. vivax (mono) and P. vivax/P. falciparum (mixed) infections. Therefore, to achieve malaria elimination goals, strengthening routine malaria diagnostic methods by implementing diagnostic tools with a good performance in detecting and accurately identifying malaria species in clinical settings is recommended.
Article
Infectious Diseases
Mahamat Souleymane Issa, Marian Warsame, Moussa Hassane Taisso Mahamat, Issakha Diar Mahamat Saleh, Kodbsse Boulotigam, Honore Djimrassengar, Ali Haggar Issa, Ousmane Abdelkader, Manah Hassoumi, Mbanga Djimadoum, Cecile Doderer-Lang, Jean Bosco Ndihiokubwayo, Charlotte Rasmussen, Didier Menard
Summary: This study confirms the high efficacy of artemisinin-based combinations, AS-AQ and AL, in the study areas in Chad. The absence of known pfkelch13 mutations and high parasite clearance rate at day 3 suggest the absence of artemisinin resistance. The absence of pfdhfr/pfdhps quintuple or sextuple mutants supports the continued use of sulfadoxine-pyrimethamine for intermittent preventive treatment during pregnancy. However, the presence of parasites with dual pfhrp2/pfhrp3 deletions poses a potential threat to the efficacy of HRP2-based rapid diagnostic tests and requires continued surveillance.
Article
Parasitology
Edvige Perrotti, Mariangela L'Episcopia, Michela Menegon, Irene S. Soares, Angel Rosas-Aguirre, Niko Speybroeck, Alejandro LLanos-Cuentas, Didier Menard, Marcelo Urbano Ferreira, Carlo Severini
Summary: This study investigates the genetic diversity of the pvetramp11.2 gene in parasite populations from the Amazonian region and worldwide. The low variability of the sequence encoding PvETRAMP11.2 protein suggests its suitability as a marker in serodiagnostic assays and vaccine design. The study also explores B-cell epitopes and their polymorphisms.
PARASITES & VECTORS
(2023)
Article
Infectious Diseases
Abnet Abebe, Isabelle Bouyssou, Solenne Mabilotte, Sisay L. Dugassa, Ashenafi Assefa, Jonathan L. Juliano, Eugenia Lo, Didier L. Menard, Lemu Golassa
Summary: This study evaluated the Duffy status of P. vivax-infected patients in different malaria endemic areas in Ethiopia. The results showed that Duffy-negative individuals are not fully protected against P. vivax infection, and these infections in Duffy negatives are often associated with low parasitemia, potentially serving as hidden reservoirs of transmission in Ethiopia.
PLOS NEGLECTED TROPICAL DISEASES
(2023)
Article
Public, Environmental & Occupational Health
Antia Figueroa-Romero, Daniel Bissombolo, Martin Meremikwu, Arsene Ratsimbasoa, Charfudin Sacoor, Iwara Arikpo, Elsha Lemba, Abel Nhama, Rianasoambolanoro Rakotosaona, Mireia Llach, Clara Pons-Duran, Sergi Sanz, Laurence Ma, Cecile Doderer-Lang, Christina Maly, Elaine Roman, Franco Pagnoni, Alfredo Mayor, Didier Menard, Raquel Gonzalez, Clara Menendez
Summary: The study aimed to assess the effect of community delivery of intermittent preventive treatment of malaria in pregnancy (C-IPTp) on the development of Plasmodium falciparum resistance to sulfadoxine-pyrimethamine. The results showed that C-IPTp did not increase the prevalence of molecular markers associated with sulfadoxine-pyrimethamine resistance after three years of implementation.
LANCET GLOBAL HEALTH
(2023)