Functional Trade-Offs in Promiscuous Enzymes Cannot Be Explained by Intrinsic Mutational Robustness of the Native Activity

Catalytic Promiscuity of Ancestral Esterases and Hydroxynitrile Lyases

(2016) Titu Devamani et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Panoramic view of a superfamily of phosphatases through substrate profiling

(2015) Hua Huang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Directed evolution of λ integrase activity and specificity by genetic derepression

(2015) Jia Wei Siau et al.   PROTEIN ENGINEERING DESIGN & SELECTION

The origins of mutational robustness

(2015) Mario A. Fares   TRENDS IN GENETICS

A highly selective biosynthetic pathway to non-natural C50 carotenoids assembled from moderately selective enzymes

(2015) Maiko Furubayashi et al.   Nature Communications

Transhydrogenase Promotes the Robustness and Evolvability of E. coli Deficient in NADPH Production

(2015) Hsin-Hung Chou et al.   PLoS Genetics

Reverse evolution leads to genotypic incompatibility despite functional and active site convergence

(2015) Miriam Kaltenbach et al.   eLife

Enzyme architecture: on the importance of being in a protein cage

(2014) John P Richard et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

The robustness and innovability of protein folds

(2014) Ágnes Tóth-Petróczy et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Substrate scope and selectivity in offspring to an enzyme subjected to directed evolution

(2014) Cecilia Blikstad et al.   FEBS Journal

Enzyme Promiscuity: Engine of Evolutionary Innovation

(2014) Chetanya Pandya et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Connectivity between Catalytic Landscapes of the Metallo-β-Lactamase Superfamily

(2014) Florian Baier et al.   JOURNAL OF MOLECULAR BIOLOGY

A Systematic Survey of an Intragenic Epistatic Landscape

(2014) Claudia Bank et al.   MOLECULAR BIOLOGY AND EVOLUTION

Differential Effects of a Mutation on the Normal and Promiscuous Activities of Orthologs: Implications for Natural and Directed Evolution

(2014) Akhil Khanal et al.   MOLECULAR BIOLOGY AND EVOLUTION

Polyspecific pyrrolysyl-tRNA synthetases from directed evolution

(2014) Li-Tao Guo et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The Robustness and Evolvability of Transcription Factor Binding Sites

(2014) J. L. Payne et al.   SCIENCE

The Robustness of a Signaling Complex to Domain Rearrangements Facilitates Network Evolution

(2014) Paloma M. Sato et al.   PLOS BIOLOGY

Large-Scale Determination of Sequence, Structure, and Function Relationships in Cytosolic Glutathione Transferases across the Biosphere

(2014) Susan T. Mashiyama et al.   PLOS BIOLOGY

Specificity in Transition State Binding: The Pauling Model Revisited

(2013) Tina L. Amyes et al.   BIOCHEMISTRY

GroEL/ES Buffering and Compensatory Mutations Promote Protein Evolution by Stabilizing Folding Intermediates

(2013) Kirsten T. Wyganowski et al.   JOURNAL OF MOLECULAR BIOLOGY

Molecular Engineering of Organophosphate Hydrolysis Activity from a Weak Promiscuous Lactonase Template

(2013) Monika M. Meier et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Precision is essential for efficient catalysis in an evolved Kemp eliminase

(2013) Rebecca Blomberg et al.   NATURE

Evolution of a designed retro-aldolase leads to complete active site remodeling

(2013) Lars Giger et al.   Nature Chemical Biology

The role of mutational robustness in RNA virus evolution

(2013) Adam S. Lauring et al.   NATURE REVIEWS MICROBIOLOGY

Tracing Determinants of Dual Substrate Specificity in Glycoside Hydrolase Family 5

(2012) Zhiwei Chen et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Evolutionary transitions to new DNA methyltransferases through target site expansion and shrinkage

(2012) Liat Rockah-Shmuel et al.   NUCLEIC ACIDS RESEARCH

Intramolecular Epistasis and the Evolution of a New Enzymatic Function

(2012) Sajid Noor et al.   PLoS One

The role of robustness in phenotypic adaptation and innovation

(2012) A. Wagner   PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES

Diminishing returns and tradeoffs constrain the laboratory optimization of an enzyme

(2012) Nobuhiko Tokuriki et al.   Nature Communications

Exploring the Evolution of Novel Enzyme Functions within Structurally Defined Protein Superfamilies

(2012) Nicholas Furnham et al.   PLoS Computational Biology

Divergent Evolution in Enolase Superfamily: Strategies for Assigning Functions

(2011) John A. Gerlt et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Fitness Trade-Offs in the Evolution of Dihydrofolate Reductase and Drug Resistance in Plasmodium falciparum

(2011) Marna S. Costanzo et al.   PLoS One

The causes of epistasis

(2011) J. A. G. M. de Visser et al.   PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES

A Dynamic Knockout Reveals That Conformational Fluctuations Influence the Chemical Step of Enzyme Catalysis

(2011) G. Bhabha et al.   SCIENCE

Initial Mutations Direct Alternative Pathways of Protein Evolution

(2011) Merijn L. M. Salverda et al.   PLoS Genetics

Enzyme Promiscuity: A Mechanistic and Evolutionary Perspective

(2010) Olga Khersonsky and Dan S. Tawfik   Annual Review of Biochemistry

What makes an enzyme promiscuous?

(2010) Ann Babtie et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Messy biology and the origins of evolutionary innovations

(2010) Dan S Tawfik   Nature Chemical Biology

Mutational effects and the evolution of new protein functions

(2010) Misha Soskine et al.   NATURE REVIEWS GENETICS

Mutational Robustness of Ribosomal Protein Genes

(2010) P. A. Lind et al.   SCIENCE

Robustness and Evolvability

(2010) Joanna Masel et al.   TRENDS IN GENETICS

Evolutionary Trajectories of Beta-Lactamase CTX-M-1 Cluster Enzymes: Predicting Antibiotic Resistance

(2010) Ângela Novais et al.   PLoS Pathogens

Stability effects of mutations and protein evolvability

(2009) Nobuhiko Tokuriki et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Chaperonin overexpression promotes genetic variation and enzyme evolution

(2009) Nobuhiko Tokuriki et al.   NATURE

An epistatic ratchet constrains the direction of glucocorticoid receptor evolution

(2009) Jamie T. Bridgham et al.   NATURE

Designer enzymes for glycosphingolipid synthesis by directed evolution

(2009) Susan M Hancock et al.   Nature Chemical Biology

In the light of directed evolution: Pathways of adaptive protein evolution

(2009) J. D. Bloom et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Conformational sampling, catalysis, and evolution of the bacterial phosphotriesterase

(2009) C. J. Jackson et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Evolutionary History of a Specialized P450 Propane Monooxygenase

(2008) Rudi Fasan et al.   JOURNAL OF MOLECULAR BIOLOGY

Expanded molecular diversity generation during directed evolution by trinucleotide exchange (TriNEx)

(2008) Amy J. Baldwin et al.   NUCLEIC ACIDS RESEARCH

A compromise required by gene sharing enables survival: Implications for evolution of new enzyme activities

(2008) S. Y. McLoughlin et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA