Article
Biochemistry & Molecular Biology
Paras Jain, Sugandha Bhatia, Erik W. Thompson, Mohit Kumar Jolly
Summary: Phenotypic heterogeneity is a hallmark of aggressive cancer behavior and a clinical challenge. This study suggests that fluctuations or noise in content duplication and partitioning of the SNAIL gene during cell division can explain spontaneous phenotypic switching and dynamic heterogeneity in PMC42-LA cells.
Article
Pharmacology & Pharmacy
Hongchao Zhang, Guopei Zhang, Jingyue Zhang, Mingyang Xiao, Su Cui, Shengwen Wu, Cuihong Jin, Jinghua Yang, Xiaobo Lu
Summary: Tobacco, specifically nicotine, plays a significant role in the development and progression of lung cancer through the induction of epithelial-mesenchymal transition (EMT). This study reveals the interplay between PPP1R13L and SP1 in nicotine-induced EMT, showing that PPP1R13L inhibits the transcriptional activity of SP1 while also being transcriptionally activated by SP1 in a feedback loop. The expression of PPP1R13L and SP1 is associated with the clinicopathological characteristics of lung cancer patients.
BIOCHEMICAL PHARMACOLOGY
(2022)
Article
Oncology
Meredith S. Brown, Kristen E. Muller, Diwakar R. Pattabiraman
Summary: The epithelial-to-mesenchymal transition (EMT) and its reversal, the mesenchymal-to-epithelial transition (MET), play critical roles in the metastasis of breast cancer and other solid tumors. However, defining and quantifying EMT status is not commonly used in clinical practice. Therefore, identifying and measuring the presence of different EMT states in tumors is a crucial first step in evaluating patient prognosis.
Article
Biochemical Research Methods
Ayalur Raghu Subbalakshmi, Sarthak Sahoo, Prakruthi Manjunatha, Shaurya Goyal, Vignesh A. Kasiviswanathan, Yeshwanth Mahesh, Soundharya Ramu, Isabelle McMullen, Jason A. A. Somarelli, Mohit Kumar Jolly
Summary: ELF3 is a factor strongly associated with an epithelial phenotype and is inhibited during EMT. It inhibits the progression of EMT and can counteract EMT induction even in the presence of EMT-inducing factors. The prognostic capacity of ELF3 is specific to cell-of-origin or lineage.
JOURNAL OF BIOLOGICAL ENGINEERING
(2023)
Article
Rheumatology
Daji Wang, Fan Zhang, Jing He, Chuyi Li, Xingjiao Liu, Meng Zhang, Haihui Huang, Zuying Xiong, Hongxia Duan, Xiaoyan Huang, Meiying Wang
Summary: This study measured the expression of vimentin and its phosphorylated forms in lupus nephritis (LN) and found that vimentin phosphorylated on Ser72 is important for renal epithelial cell migration, which could enhance the progression of vimentin-induced epithelial-mesenchymal transition during LN development.
INTERNATIONAL JOURNAL OF RHEUMATIC DISEASES
(2023)
Article
Biochemistry & Molecular Biology
Xiaoru Zhang, Vinay Singh Tanwar, Cynthia C. Jose, Hyun-Wook Lee, Suresh Cuddapah
Summary: This study demonstrates that nickel exposure leads to persistent downregulation of E-cadherin by causing loss of Sp1 binding at the CDH1 promoter, which induces EMT. Continuous upregulation of ZEB1 is also a key factor in the loss of E-cadherin, and knocking out ZEB1 using CRISPR-Cas9 technology can restore E-cadherin expression.
MOLECULAR CARCINOGENESIS
(2022)
Article
Medicine, Research & Experimental
Zongji Zheng, Shuting Zhang, Jiaqi Chen, Meina Zou, Yanlin Yang, Wen Lu, Shijing Ren, Xiangyu Wang, Wenhui Dong, Zikun Zhang, Ling Wang, Meiping Guan, Gladys L. Y. Cheing, Yaoming Xue, Yijie Jia
Summary: The feedback loop formed by HDAC2 and miR-205 plays a crucial role in regulating ECM production in tubular epithelial cells in individuals with DKD, suggesting its significance in the pathogenesis of DKD.
Article
Oncology
Jun Wang, Shaobo Ouyang, Siyu Zhao, Xianhua Zhang, Mingyang Cheng, Xin Fan, Ying Cai, Lan Liao
Summary: circFAM126A is significantly upregulated in oral squamous cell carcinoma (OSCC) and modulates the growth and epithelial-mesenchymal transition (EMT) of OSCC cells through the miR-186/FUS/RAB41 axis.
FRONTIERS IN ONCOLOGY
(2022)
Article
Biochemical Research Methods
Yingying Ma, Songbiao Zhu, Meiqi Yi, Wenhao Zhang, Yuanyuan Xue, Xiaohui Liu, Haiteng Deng
Summary: Protein S-glutathionylation is an important post-translational modification that regulates cellular processes. This study investigated the changes in glutathionylome during epithelial-mesenchymal transition (EMT) and found that the glutathionylation level of 15-hydroxyprostaglandin dehydrogenase (15-PGDH) increased, inhibiting its enzymatic activity and promoting EMT.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Wen-Wei Chang, Bing-Yen Wang, Shih-Hong Chen, Peng-Ju Chien, Gwo-Tarng Sheu, Ching-Hsiung Lin
Summary: This study found that in pemetrexed-resistant NSCLC cells, overexpression of BMI1 leads to downregulation of miR-145-5p, resulting in increased expression of Sp1 and induction of the EMT process. Increasing miR-145-5p expression could serve as a sensitizing agent for pemetrexed-resistant NSCLC patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Baris Kucukkaraduman, Ekin Gokce Cicek, Muhammad Waqas Akbar, Secil Demirkol Canli, Burcak Vural, Ali Osmay Gure
Summary: This study investigated the use of eight natural compounds and two repurposed agents on cancer cells, with curcumin showing the most significant anti-cancer activity across different cell lines. While some natural products induced MET in cancer cells, the MET induction did not necessarily enhance chemosensitivity.
Article
Immunology
Zhishang Meng, Yanzhu Chen, Wenyi Wu, Bin Yan, Lusi Zhang, Huihui Chen, Yongan Meng, Youling Liang, Xiaoxi Yao, Jing Luo
Summary: This study identified PRRX1 as a reliable TME-related biomarker that can predict survival and provide new insights into therapy for uveal melanoma (UM) patients. The results showed that increased PRRX1 expression is independently associated with poorer overall survival and metastasis-free survival in UM patients, and PRRX1 promotes malignant progression by facilitating epithelial-mesenchymal transition. These findings suggest that PRRX1 may be a potential target for UM therapy.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Pharmacology & Pharmacy
Andrea Karolin, Genevieve Escher, Stefan Rudloff, Daniel Sidler
Summary: The study revealed that cyclosporine A acts independently of NFAT on kidney epithelial cells. Moreover, inhibition of serine/threonine protein kinases mimics cyclosporine A's activity on kidney epithelial cells.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Deguang Kong, Hengbo Zhou, Deepika Neelakantan, Connor J. Hughes, Jessica Y. Hsu, Ramakrishnan Rajaram Srinivasan, Michael T. Lewis, Heide L. Ford
Summary: VEGF-C secreted by EMT breast cancer cells can promote proliferation, migration, and invasion of epithelial breast cancer cells through non-canonical activation of GLI signaling. Inhibiting VEGF-C in EMT cells or knocking down NRP2 in neighboring epithelial cancer cells disrupts the aggressive phenotypes induced by EMT cells, suggesting a novel therapeutic target for metastasis in breast cancer.
Article
Multidisciplinary Sciences
Michela Serresi, Sonia Kertalli, Lifei Li, Matthias Jurgen Schmitt, Yuliia Dramaretska, Jikke Wierikx, Danielle Hulsman, Gaetano Gargiulo
Summary: Research has shown that chromatin regulators have a broader impact on EMT interconversion in lung cancer cells than kinases, and the loss of ARID1A, DOT1L, BRD2, and ZMYND8 may have nondeterministic and sometimes opposite consequences. These findings reveal general principles underlying transcriptional control of cancer cell plasticity.
Article
Cardiac & Cardiovascular Systems
Kiran A. Vaidya, Matthew P. Donnelly, Terence W. Gee, Marine-Ayan Ibrahim Aibo, Stephen Byers, Jonathan T. Butcher
CARDIOVASCULAR PATHOLOGY
(2020)
Article
Multidisciplinary Sciences
Maria C. Palumbo, Lisa Q. Rong, Jiwon Kim, Pedram Navid, Razia Sultana, Jonathan Butcher, Alberto Redaelli, Mary J. Roman, Richard B. Devereux, Leonard N. Girardi, Mario F. L. Gaudino, Jonathan W. Weinsaft
Article
Biophysics
Huseyin Enes Salman, Maha Alser, Akshay Shekhar, Russell A. Gould, Fatiha M. Benslimane, Jonathan T. Butcher, Huseyin C. Yalcin
Summary: This study investigates disturbed hemodynamics and deteriorated cardiac growth following left atrial ligation (LAL) to explore potential mechanobiological mechanisms for the embryonic development of hypoplastic left heart syndrome (HLHS), highlighting the critical importance of hemodynamic disturbances during heart valve and ventricle development.
BIOMECHANICS AND MODELING IN MECHANOBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Shaohua Wu, Vikas Kumar, Peng Xiao, Mitchell Kuss, Jung Yul Lim, Chittibabu Guda, Jonathan Butcher, Bin Duan
SCIENTIFIC REPORTS
(2020)
Article
Engineering, Biomedical
Terence W. Gee, Jennifer M. Richards, Ablajan Mahmut, Jonathan T. Butcher
Summary: This study utilized a mechanically stressed 3D culture system to replicate valve interstitial cell-induced matrix calcification and investigated the collaborative interactions between valve endothelial cells and interstitial cells in calcific pathogenesis. Results showed that endothelial cells induce pathological remodeling and calcification of interstitial cells through EndMT and paracrine signaling.
Review
Cardiac & Cardiovascular Systems
Katherine Driscoll, Alexander D. Cruz, Jonathan T. Butcher
Summary: Calcific aortic valve disease is on the rise globally, but treatment is limited to prosthetic replacement. With a growing number of younger and more active patients, alternative therapies are needed. There is potential for biologically based diagnostics and treatments to help alleviate or delay disease progression, but a limited understanding of the disease mechanisms hinders progress towards clinical interventions.
CIRCULATION RESEARCH
(2021)
Article
Multidisciplinary Sciences
Michael F. Z. Wang, Madhav Mantri, Shao-Pei Chou, Gaetano J. Scuderi, David W. McKellar, Jonathan T. Butcher, Charles G. Danko, Iwijn De Vlaminck
Summary: Conventional single-cell RNA sequencing analysis relies on genome annotations that may be incomplete or inaccurate especially for understudied organisms. Here the authors present a bioinformatic tool that leverages single-cell data to uncover biologically relevant transcripts beyond the best available genome annotation.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Madhav Mantri, Gaetano J. Scuderi, Roozbeh Abedini-Nassab, Michael F. Z. Wang, David McKellar, Hao Shi, Benjamin Grodner, Jonathan T. Butcher, Iwijn De Vlaminck
Summary: By utilizing single-cell and spatial transcriptomics in chicken hearts, the authors conducted a census of cellular interactions from early to late four-chambered heart stage, identifying a distinct epicardial-mesenchymal cell population with a migratory phenotype.
NATURE COMMUNICATIONS
(2021)
Article
Engineering, Biomedical
Stephanie E. Lindsey, Irene E. Vignon-Clementel, Jonathan T. Butcher
Summary: Mechanical forces play a crucial role in the growth and remodeling of primitive pharyngeal arch arteries into the heart's great vessels. However, the direct correlation between hemodynamics and PAA morphogenesis has been poorly understood. A study using minimally invasive occlusion experiments in avian embryos and 3D anatomical models revealed significant changes in morphology and hemodynamics following vessel occlusion, providing insights into the dynamic vessel adaptation process.
ANNALS OF BIOMEDICAL ENGINEERING
(2021)
Article
Developmental Biology
David Bassen, Mingkun Wang, Duc Pham, Shuofei Sun, Rashmi Rao, Rishabh Singh, Jonathan Butcher
Summary: This study investigated the influence of compressive and tensile stresses on valve growth and shape maturation, identifying a mechanically activated switch between valve growth and maturation. Compressive stress was found to drive cell proliferation via BMP signaling, while tensile stress promoted valve maturation through MLC2 contractility. The effects of osmotic stress on valve development were shown to be conserved through different stages.
Article
Anatomy & Morphology
Duc H. Pham, Charles R. Dai, Belle Y. Lin, Jonathan T. Butcher
Summary: The study found that the elongation of the fetal semilunar valve occurs through fibrosa-sided endocardial proliferation. Low shear stress induces canonical Wnt signaling in fetal valve endothelium, driving endocardial proliferation, while high shear stress activates Notch signaling to inhibit Wnt signaling. This novel mechanically regulated molecular switch explains how fluid shear stress guides the growth direction of valve endothelium.
DEVELOPMENTAL DYNAMICS
(2022)
Article
Engineering, Biomedical
Nianfang Ma, Daniel Y. Cheung, Jonathan T. Butcher
Summary: In this study, a novel photocrosslinkable composite hydrogel mNCC-MeGel (mNG) was fabricated and evaluated for heart valve applications. Encapsulated HADMSC in mNG showed support for a quiescent fibroblastic phenotype and resistance towards calcification, making it an attractive biomaterial for TEHV. Additionally, mNG was successfully used for 3D bioprinting a tall tubular structure that sustained cell viability, demonstrating its potential for tissue engineering.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2022)
Review
Biochemistry & Molecular Biology
Anirikh Chakrabarti, Lucie Geurts, Lesley Hoyles, Patricia Iozzo, Aletta D. Kraneveld, Giorgio La Fata, Michela Miani, Elaine Patterson, Bruno Pot, Colette Shortt, David Vauzour
Summary: The link between the gut and brain has attracted increasing scientific interest in the past decade, with a focus on the role of gut microbiota in gut-brain communication. Association studies have explored the potential influence of gut microbiota on memory, learning, anxiety, stress, and neurodevelopmental and neurodegenerative disorders. However, the triggers and mechanisms underlying this influence are still largely unknown, and most evidence comes from pre-clinical studies.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Multidisciplinary Sciences
Emily J. Farrar, Emilye Hiriart, Ablajan Mahmut, Bernd Jagla, David S. Peal, David J. Milan, Jonathan T. Butcher, Michel Puceat
Summary: Studies have found that a subset of mouse endocardial cells express Oct4 induced by nuclear factor kappa B (NF kappa B) during the process of transition starting at day 9.5 of embryo development. These cells acquire a chondro-osteogenic fate and in adulthood lead to valve calcification. Genetic deletion of OCT4 can prevent aortic stenosis and valve calcification.
Correction
Engineering, Biomedical
Sraeyes Sridhar, Duc H. Pham, Terence W. Gee, Joanna Hua, Jonathan T. Butcher
CURRENT OPINION IN BIOMEDICAL ENGINEERING
(2021)