Article
Microbiology
Miguel Diaz-Guerrero, Meztlli O. Gaytan, Eduardo Soto, Norma Espinosa, Elizabeth Garcia-Gomez, Arely Marcos-Vilchis, Angel Andrade, Bertha Gonzalez-Pedrajo
Summary: The type III secretion system (T3SS) is a complex molecular device that enables pathogenic bacteria to transport effector proteins directly into host cells. The CesL/SepL/SepD complex regulates the switch from translocator to effector secretion in enteropathogenic Escherichia coli, ensuring proper secretion and stability of the components involved. CesL also interacts with the cytoplasmic domains of export gate components, contributing to the regulation of substrate secretion.
Review
Biochemistry & Molecular Biology
Amit Meir, Kevin Mace, Yogesh Vegunta, Sunanda M. Williams, Gabriel Waksman
Summary: This review provides an update on recent advances in substrate recruitment and delivery by recruitment platforms associated with Type III, IV, and VI secretion systems in gram-negative bacteria.
TRENDS IN MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Alina Guse, Manuel Halte, Svenja Huesing, Marc Erhardt
Summary: The study revealed that the type-III secretion system of bacterial flagellum has a remarkable substrate specificity during flagellar assembly, accurately secreting the required substrates at different stages, and the specificity switch is unidirectional and irreversible.
MOLECULAR MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Lu-Lu He, Xin Wang, Dylan O'Neill Rothenberg, Xiaoli Xu, Hai-Hong Wang, Xin Deng, Zi-Ning Cui
Summary: In this study, a high-throughput screening reporter system was constructed to screen for imidazole, oxazole, and thiazole compounds. The screening identified three compounds that significantly inhibited the activity of hrpW and hrpL gene promoters in P. syringae without affecting its growth. These compounds were found to suppress the expression of T3SS genes and inhibit the pathogenicity of P. syringae in tomato and bean. The findings suggest the potential of novel P. syringae T3SS inhibitors for disease prevention and control.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Julie Ming Liang, Lisa Ann Burdette, Han Teng Wong, Danielle Tullman-Ercek
Summary: Protein production using bacteria is costly and inefficient. Researchers have engineered a bacterial secretion system to improve the production process by directly secreting soluble proteins into the culture media. They explored different activation methods and created strains capable of efficiently producing heterologous proteins. They also discovered a genetic relationship between T3SS regulators, hilE and hilD, further enhancing the industrial competitiveness of protein production strains.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Stephan Wimmi, Alexander Balinovic, Hannah Jeckel, Lisa Selinger, Dimitrios Lampaki, Emma Eisemann, Ina Meuskens, Dirk Linke, Knut Drescher, Ulrike Endesfelder, Andreas Diepold
Summary: Current research shows that in gastrointestinal pathogens, the external pH plays a crucial role in regulating the assembly of T3SS cytosolic components, preventing T3SS activity in the stomach and facilitating its reactivation in the intestine.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Marc-Andre Leblanc, Morgan R. Fink, Thomas T. Perkins, Marcelo C. Sousa
Summary: The study shows that effector proteins from certain bacteria can be injected into host cells through a type III secretion system. These proteins need to be partially unfolded to pass through the narrow channel. Mechanical unfolding of the proteins revealed that they exhibit low force and high mechanical compliance, facilitating efficient secretion.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Biochemistry & Molecular Biology
Julia Sanchez-Garrido, David Ruano-Gallego, Jyoti S. Choudhary, Gad Frankel
Summary: Recent studies have shown that T3SS effectors interact with each other in the host, revealing their interdependency and context-dependent essentiality, which is of great significance for understanding the pathogenesis of the pathogens.
TRENDS IN MICROBIOLOGY
(2022)
Article
Microbiology
Kevin Munoz Navarrete, Ladislav Bumba, Tatyana Prudnikova, Ivana Malcova, Tania Romero Allsop, Peter Sebo, Jana Kamanova
Summary: The study reveals that BopN is a gatekeeper of the Bordetella T3SS, responsible for regulating the secretion and translocation of the BteA effector into host cells. It is similar to gatekeepers that control access to the T3SS channel from the bacterial cytoplasm. Deletion of the bopN gene in B. bronchiseptica increased secretion of BteA, but decreased the translocation of BteA into host cell cytoplasm.
MICROBIOLOGY SPECTRUM
(2023)
Review
Microbiology
Elizabeth A. Rucks
Summary: Type III secretion systems (T3SS) are utilized by Gram-negative pathogens to deliver effector proteins into target eukaryotic cells for manipulating cell functions and enhancing pathogenesis. This review will discuss the history, biochemical characterization, and function of chlamydial T3SS, as well as the use of heterologous/surrogate models for studying it. The review will also cover the history of chlamydial effectors and recent advances in the field.
MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS
(2023)
Article
Microbiology
Anqun Hu, Ming Hu, Shanshan Chen, Yang Xue, Xu Tan, Jianuan Zhou
Summary: The study identified five compounds that can effectively inhibit soft rot symptoms caused by Dickeya zeae, reduce hypersensitive response on non-host plants, and downregulate the expression of T3SS, providing potential biocontrol agents for the prevention of soft rot disease.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Eduardo Soto, Jorge E. Galan, Marfa Lara-Tejero
Summary: In this study, an extensive in vivo cross-linking strategy aided by structure modeling was used to investigate the sorting platform complex of type III secretion systems. The assembly process of this bacterial structure was mapped using identified cross-links as signatures for pairwise intersubunit interactions and systematic genetic deletions. Insights generated by this study could be used for the rational development of antivirulence strategies against medically important bacterial pathogens.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Gastroenterology & Hepatology
Ce Zhang, Min Liu, Ying Wu, Xixi Li, Chen Zhang, Douglas R. Call, Ming Liu, Zhe Zhao
Summary: In Vibrio parahaemolyticus, the expression of T3SS1 is regulated by a complex quorum sensing phosphorelay circuit involving the hybrid histidine kinase ArcB, sensor kinase LuxQ, and response regulator LuxO. The study shows that LuxQ can directly interact with ArcB under high oxygen conditions, leading to phosphorylation of LuxO and activation of T3SS1 expression. Under hypoxic conditions, ArcB autophosphorylates and phosphorylates ArcA, but ArcA does not participate in regulating T3SS1 expression.
Article
Biochemistry & Molecular Biology
Bailey Milne-Davies, Stephan Wimmi, Andreas Diepold
Summary: The type III secretion system is the common core of two bacterial molecular machines: the flagellum and the injectisome. It is characterized by flexibility, adaptivity, and a dynamic nature.
MOLECULAR MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Sean Miletic, Dirk Fahrenkamp, Nikolaus Goessweiner-Mohr, Jiri Wald, Maurice Pantel, Oliver Vesper, Vadim Kotov, Thomas C. Marlovits
Summary: The study suggests that pathogenic bacteria can inject effector proteins directly into host cell cytoplasm through virulent type III secretion systems or injectisomes. Structures of a needle complex engaged with the effector protein reveal the complete secretion channel and provide insights into the mechanism of substrate translocation through T3SSs.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Medicinal
Maulik K. Nariya, Jae Hyun Kim, Jian Xiong, Peter A. Kleindl, Asha Hewarathna, Adam C. Fisher, Sangeeta B. Joshi, Christian Schoneich, M. Laird Forrest, C. Russell Middaugh, David B. Volkin, Eric J. Deeds
JOURNAL OF PHARMACEUTICAL SCIENCES
(2017)
Article
Chemistry, Medicinal
Peter A. Kleindl, Jian Xiong, Asha Hewarathna, Olivier Mozziconacci, Maulik K. Nariya, Adam C. Fisher, Eric J. Deeds, Sangeeta B. Joshi, C. Russell Middaugh, Christian Schoneich, David B. Volkin, M. Laird Forrest
JOURNAL OF PHARMACEUTICAL SCIENCES
(2017)
Article
Chemistry, Medicinal
Asha Hewarathna, Olivier Mozziconacci, Maulik K. Nariya, Peter A. Kleindl, Jian Xiong, Adam C. Fisher, Sangeeta B. Joshi, C. Russell Middaugh, M. Laird Forrest, David B. Volkin, Eric J. Deeds, Christian Schoneich
JOURNAL OF PHARMACEUTICAL SCIENCES
(2017)
Article
Biochemical Research Methods
Abhishek Mallela, Maulik K. Nariya, Eric J. Deeds
PLOS COMPUTATIONAL BIOLOGY
(2020)
Article
Biochemical Research Methods
Denis Schapiro, Artem Sokolov, Clarence Yapp, Yu-An Chen, Jeremy L. Muhlich, Joshua Hess, Allison L. Creason, Ajit J. Nirmal, Gregory J. Baker, Maulik K. Nariya, Jia-Ren Lin, Zoltan Maliga, Connor A. Jacobson, Matthew W. Hodgman, Juha Ruokonen, Samouil L. Farhi, Domenic Abbondanza, Eliot T. McKinley, Daniel Persson, Courtney Betts, Shamilene Sivagnanam, Aviv Regev, Jeremy Goecks, Robert J. Coffey, Lisa M. Coussens, Sandro Santagata, Peter K. Sorger
Summary: MCMICRO is a modular and open-source computational pipeline that enables the transformation of highly multiplexed tissue whole-slide images into single-cell data. It is versatile and can be used with various imaging platforms, maintaining spatial context and providing a foundation for the continued development of tissue imaging software.