Review
Biochemistry & Molecular Biology
Massimo Carossa, Davide Cavagnetto, Francesca Mancini, Alessandro Mosca Balma, Federico Mussano
Summary: This paper reviewed the cellular activity on titanium samples exposed to Plasma of Argon (PoA) treatment. The results showed potential effects of PoA in promoting cell adhesion and protein adsorption, but the duration of this effect remains unclear. However, the overall quality of the analyzed studies was poor, and only one in vivo study was conducted. Further research is needed to demonstrate the long-term efficacy of this treatment and support its use in clinical practice.
Article
Microbiology
Yanyan Fu, Sandra Maass, Francis M. Cavallo, Anne de Jong, Erwin Raangs, Johanna Westra, Girbe Buist, Doerte Becher, Jan Maarten van Dijl
Summary: Periodontitis is an inflammatory disease that causes tooth loss by destroying the supporting tissues around the teeth. Aggregatibacter actinomycetemcomitans (Aa) is a causative agent of periodontitis and is also associated with nonoral diseases. This study identifies serotype-specific differences in the production of virulence factors by clinical Aa isolates, which may lead to new approaches in preventing and treating periodontitis and its associated diseases.
MICROBIOLOGY SPECTRUM
(2023)
Article
Oncology
Ardavan Etemadi, Seyed Shahabodin Hashemi, Nasim Chiniforush
Summary: The study evaluated the effect of Photodynamic therapy with Curcumin and Riboflavin on the decontamination of implant surfaces. The results showed that Photodynamic therapy with Curcumin significantly reduced the bacterial colonies on the implant surfaces, making it an effective method for reducing biofilm.
PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY
(2023)
Article
Medicine, General & Internal
Mark Lindholm, Rolf Claesson, Arthur Kemoli, Tonnie Mulli, Jan Oscarsson, Dorte Haubek, Anders Johansson
Summary: The study investigated the prevalence of Aa and A. aphrophilus in Maasai adolescents in Kenya, as well as the effect of herbal plants on leukotoxicity. Results showed a high prevalence of Aa, with a low frequency of the JP2 genotype, and a widespread presence of A. aphrophilus among the sampled individuals. Additionally, the commonly used herbal plant Warburgia ugandensis was effective in inhibiting Aa leukotoxicity.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Microbiology
Yanyan Fu, Sandra Maass, Marines du Teil Espina, Anouk H. G. Wolters, Yanan Gong, Anne de Jong, Erwin Raangs, Girbe Buist, Johanna Westra, Dorte Becher, Jan Maarten van Dijl
Summary: This study compared the extracellular proteome of different isolates and derivative strains of Aggregatibacter actinomycetemcomitans to investigate its virulence. The results showed that a limited number of extracellular proteins play central roles in the pathogenesis of A. actinomycetemcomitans, suggesting that they could be potential targets for preventing or treating oral diseases.
Article
Cell Biology
Mariana Rates Gonzaga Santos, Ian de Meira Chaves, Celso Martins Queiroz-Junior, Allysson Thiago Cramer, Thales Augusto Anestino, Anna Clara Paiva Menezes dos Santos, Paulo Gaio Leite, Soraia Macari, Breno Rocha Barrioni, Marivalda de Magalhaes Pereira, Mauro Martins Teixeira, Danielle da Gloria de Souza, Mila Fernandes Moreira Madeira, Fabiana Simao Machado
Summary: This study investigated the role of SOCS2 in Aa-induced alveolar bone loss. Alveolar bone loss was induced by infecting mice with Aa and bone parameters, cell counts, and cytokine profile were evaluated. Results showed that SOCS2 deficiency increased alveolar bone loss despite decreased proinflammatory cytokine production. Therefore, SOCS2 is a regulator of Aa-induced bone loss and an important target for preventing alveolar bone loss in periodontal inflammatory conditions.
INFLAMMATION RESEARCH
(2023)
Article
Multidisciplinary Sciences
Maryam Pourhajibagher, Abbas Bahador
Summary: This study focused on the use of photo-sonodynamic antimicrobial chemotherapy (PSACT) to attenuate the virulence of Aggregatibacter actinomycetemcomitans. The Cur-NPhs-PSACT showed dose-dependent antimicrobial effects, with high concentrations leading to significant reductions in cell viability, biofilm killing/degradation, metabolic activity, and expression of virulence determinant genes. This novel approach may have potential in decreasing periodontal disease severity in patients.
SCIENTIFIC REPORTS
(2021)
Article
Pharmacology & Pharmacy
En Hyung Chang, Angela C. Brown
Summary: The study found that EGCg increased LtxA production and changed the localization of secreted LtxA from the supernatant to the surface of bacterial cells. In the co-culture model, a single low dose of EGCg did not protect host THP-1 cells from A. actinomycetemcomitans-mediated cytotoxicity, but a multiple dosing strategy had improved effects.
JOURNAL OF PHARMACY AND PHARMACOLOGY
(2021)
Article
Microbiology
Manuela R. Bueno, Karin H. Ishikawa, Gislane Almeida-Santos, Ellen S. Ando-Suguimoto, Natali Shimabukuro, Dione Kawamoto, Marcia P. A. Mayer
Summary: Probiotics may help achieve a balanced microbiome in periodontitis by interfering with the colonization of Aggregatibacter actinomycetemcomitans in the oral cavity. This in vitro study evaluated the effect of two commercially available lactobacilli strains on gingival epithelial cells infected with A. actinomycetemcomitans. The results suggest that these probiotics may reduce the ability of A. actinomycetemcomitans to interact with gingival epithelial cells and modulate the cells' response.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Immunology
Hazel Ozuna, Silvia M. Uriarte, Donald R. Demuth
Summary: This study revealed that human neutrophils can synthesize, store, and release epinephrine, which A. actinomycetemcomitans relies on as a growth signal. The bacteria promotes azurophilic granule exocytosis by neutrophils to acquire epinephrine for survival, providing new insights into the mechanism of bacterial infection in periodontitis.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Dentistry, Oral Surgery & Medicine
Nabil Khzam, Leticia Algarves Miranda, Omar Kujan, Kate Shearston, Dorte Haubek
Summary: This study investigated the global prevalence of the JP2 genotype of Aggregatibacter actinomycetemcomitans (Aa). The results showed a relatively high presence of the JP2 genotype in subjects from South America, North America, and Africa. However, there were no studies estimating the presence of the JP2 genotype in the Oceania region. Due to the heterogeneity and quality of the included publications, caution should be exercised when interpreting the data, and there is a need for additional evidence.
CLINICAL ORAL INVESTIGATIONS
(2022)
Article
Microbiology
Zeinab Razooqi, Carola Hoglund Aberg, Francis Kwamin, Rolf Claesson, Dorte Haubek, Jan Oscarsson, Anders Johansson
Summary: The study aimed to investigate the presence of two oral pathogens and their genotypes in relation to the development of periodontal disease. The results show that the presence of Aggregatibacter actinomyctemcomitans and Filifactor alocis is associated with increased periodontal pocket depth and progression of the disease.
Article
Microbiology
Yohei Nakamura, Koji Watanabe, Yoshie Yoshioka, Wataru Ariyoshi, Ryota Yamasaki
Summary: The effect of hydrogen peroxide on Aggregatibacter actinomycetemcomitans was investigated. The treatment resulted in the survival of approximately 0.5% of the bacterial population. Surviving bacteria did not acquire resistance to hydrogen peroxide but exhibited persister behavior. Sterilization with mitomycin C reduced the number of persister survivors.
Article
Medicine, General & Internal
Klara Martinsson, Andrea Di Matteo, Carina Ohman, Anders Johansson, Anna Svard, Kulveer Mankia, Paul Emery, Alf Kastbom
Summary: This study aimed to determine the presence of antibodies towards LtxA in patients at risk of developing rheumatoid arthritis (RA). The results showed that serum anti-LtxA levels were not elevated before RA diagnosis, and associations with disease progression and ACPA levels differed between populations.
FRONTIERS IN MEDICINE
(2023)
Article
Immunology
Zihan Li, Qiuxia Huang, Zhuoran Wang, Lijia Huang, Lisha Gu
Summary: Periodontitis is a dysbiosis condition caused by the coordinated action of multiple members within the microbial consortium. This study demonstrates that Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans can alter the structure and composition of subgingival microbiome, leading to dysbiosis and impaired epithelium function. P. gingivalis drives the commensal microbiome towards dysbiosis, while A. actinomycetemcomitans enhances the symbiotic state.
JOURNAL OF INFECTIOUS DISEASES
(2023)
Article
Chemistry, Multidisciplinary
Livia Nastri, Ludovica Nucci, Domenico Carozza, Stefano Martina, Ismene Serino, Letizia Perillo, Fabrizia d'Apuzzo, Vincenzo Grassia
Summary: The study aimed to evaluate gingival recessions and periodontal status in patients who underwent non-extraction orthodontic treatment and retention for at least two years. The findings suggest that non-extraction orthodontic treatment does not have a significant impact on the development of gingival recessions or periodontal health after retention.
APPLIED SCIENCES-BASEL
(2022)
Review
Chemistry, Physical
Luigi Guida, Eriberto Bressan, Gennaro Cecoro, Armando Davide Volpe, Massimo Del Fabbro, Marco Annunziata
Summary: This systematic review and meta-analysis found that short implants perform as well as longer implants in edentulous sites without the need for bone augmentation. The survival rate of short implants is similar to that of longer implants, but longer implants experience more bone loss at 1 and 5 years, while short implants have more technical complications at 10 years.
Review
Chemistry, Multidisciplinary
Gennaro Cecoro, Debora Bencivenga, Marco Annunziata, Nunzio Cennamo, Fulvio Della Ragione, Alessandro Formisano, Angelantonio Piccirillo, Emanuela Stampone, Pio Antonio Volpe, Luigi Zeni, Adriana Borriello, Luigi Guida
Summary: This scoping review evaluates the influence of magnetic field stimulation on dental implant osseointegration. In vitro studies show a positive effect of Pulsed ElectroMagnetic Fields (PEMF) on bone cell proliferation, while the effects of Static magnetic Fields from permanent Magnets (SFM) are conflicting. Both types of stimulation induce cell adhesion and osteogenic differentiation. In vivo studies demonstrate increased bone-to-implant contact rate and positive effects on implant stability. However, further preclinical and clinical studies are needed to assess the effect of magnetic fields on dental implant osseointegration more clearly.
APPLIED SCIENCES-BASEL
(2022)
Article
Dentistry, Oral Surgery & Medicine
Luigi Guida, Umberto Esposito, Massimiliano Sirignano, Paolo Torrisi, Marco Annunziata, Denis Cecchinato
Summary: The aim of this multicenter trial was to compare the effectiveness of 6mm short and 11mm long implants for the rehabilitation of completely edentulous non-atrophic mandibles. The study found that there was no significant difference in clinical and radiographic outcomes between the two types of implants when used in similar conditions.
CLINICAL ORAL IMPLANTS RESEARCH
(2023)
Article
Chemistry, Physical
Francesco Pera, Paolo Pesce, Francesco Bagnasco, Nicolo Pancini, Massimo Carossa, Lorenzo Baldelli, Marco Annunziata, Marco Migliorati, Domenico Baldi, Maria Menini
Summary: The aim of this study was to compare the accuracy of a new digital impression system with the traditional plaster impression technique in the realization of full-arch implant-supported metal frameworks. The results showed that the accuracy of the metal frameworks obtained through the digital impression system was comparable to those obtained through the traditional plaster impression technique. This is significant for promoting the clinical application of digital impression systems.
Article
Chemistry, Multidisciplinary
Roberto Sorrentino, Maria I. Di Mauro, Renato Leone, Gennaro Ruggiero, Marco Annunziata, Fernando Zarone
Summary: The study evaluated the long-term clinical performance of narrow diameter implants and different types of crowns in the treatment of maxillary lateral incisors agenesis. Marginal bone resorption and soft tissue quality were evaluated after 16 years. The overall success and survival rates of the restorations were 85% and 95% respectively, with no significant differences between metal-ceramic and all-ceramic crowns.
APPLIED SCIENCES-BASEL
(2023)
Article
Infectious Diseases
Francesco Napolitano, Concetta Paola Pelullo, Monica Lamberti, Giovanna Donnarumma, Gabriella Di Giuseppe
Summary: This study evaluates antimicrobial prescribing practices in hospitals in Italy, focusing on antibiotic appropriateness. The findings show that over one third of patients received inappropriate antibiotic prescriptions. Factors such as being female, having a high Charlson comorbidity index score, and longer hospital stays were associated with inappropriate prescriptions.
Article
Chemistry, Analytical
Nunzio Cennamo, Angelantonio Piccirillo, Debora Bencivenga, Francesco Arcadio, Marco Annunziata, Fulvio Della Ragione, Luigi Guida, Luigi Zeni, Adriana Borriello
Summary: Point-of-Care (POC) tests based on biomarkers are crucial for advancing medicine, especially in monitoring acute and chronic inflammations. Among the pro-inflammatory cytokines, interleukin 6 (IL-6) is considered one of the most important. This study presents the development of two IL-6 biosensors using a combination of the same antibody self-assembled monolayer and two different plasmonic probes. Experimental results show that these biosensors can detect IL-6 in different concentration ranges. The proposed sensing approach has the potential to be used in POC devices for diagnosing and monitoring oral and systemic diseases by detecting IL-6 in the desired concentration range.
Article
Biochemistry & Molecular Biology
Alessandra Fusco, Vittoria Savio, Donatella Cimini, Sergio D'Ambrosio, Adriana Chiaromonte, Chiara Schiraldi, Giovanna Donnarumma
Summary: This study aimed to evaluate the ability of different strains of Lactobacillus spp. to improve the integrity of the intestinal barrier, exert anti-inflammatory and immunomodulatory activity, and prevent Salmonella Typhimurium and enteroinvasive Escherichia coli (EIEC) infections. The results showed that L. brevis, L. reuteri, and L. rhamnosus were the most effective in protecting the intestinal epithelium.
Article
Engineering, Biomedical
Aishwarya Bal, Saurav Panda, Rinkee Mohanty, Anurag Satpathy, Rashmita Nayak, Margherita Tumedei, Francesca Argenta, Gianluca Colapinto, Massimo Del Fabbro, Marco Annunziata
Summary: The effectiveness of hyaluronic acid (HA) gel injection with and without plasma rich in growth factors (PRGF) for managing interdental papillary loss was evaluated in this study. The results showed significant improvement in PW, PDH, DA, and DV in both groups, with HA + PRGF group showing significantly greater improvement in PDH, DA, and DV at 6 and 12 weeks compared to HA group. Therefore, PRGF may have a significant adjuvant effect when used along with HA.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)
Article
Dentistry, Oral Surgery & Medicine
Luigi Guida, Debora Bencivenga, Marco Annunziata, Francesco Arcadio, Adriana Borriello, Fulvio Della Ragione, Alessandro Formisano, Angelantonio Piccirillo, Luigi Zeni, Nunzio Cennamo
Summary: In this study, a novel optical fiber biosensor based on surface plasmon resonance was developed to detect salivary MMP-8 protein. The biosensor had a low detection limit in both buffer and saliva, and showed high selectivity. This technology has the potential to be a highly sensitive biosensor for monitoring salivary MMP-8 levels.
JOURNAL OF DENTISTRY
(2023)
Article
Dentistry, Oral Surgery & Medicine
Marco Annunziata, Giovanna Donnarumma, Agostino Guida, Livia Nastri, Gerardo Persico, Alessandra Fusco, Ignacio Sanz-Sanchez, Luigi Guida
Summary: The objective of this randomized clinical trial was to evaluate the efficacy of repeated ICG-aPDT as an adjunct to full-mouth subgingival debridement in treating periodontitis. The study found that the combination of repeated ICG-aPDT and full-mouth subgingival debridement did not provide significant benefits compared to debridement alone, except for selective clinical and microbiological improvements.
CLINICAL ORAL INVESTIGATIONS
(2023)