The Effects of Sugars on the Biofilm Formation of Escherichia coli 185p on Stainless Steel and Polyethylene Terephthalate Surfaces in a Laboratory Model

Background: Bacteria utilize various methods in order to live in protection from adverse environmental conditions. One such method involves biofilm formation; however, this formation is dependent on many factors. The type and concentration of substances such as sugars that are present in an environment can be effective facilitators of biofilm formation. Methods: First, the physico-chemical properties of the bacteria and the target surface were studied via the MATS and contact angle measurement methods. Additionally, adhesion to different surfaces in the presence of various concentrations of sugars was compared in order to evaluate the effect of these factors on the biofilm formation of Escherichia coli, which represents a major food contaminant. Results: Results showed that the presence of sugars has no effect on the bacterial growth rate; all three concentrations of sugars were hydrophilic and demonstrated a high affinity toward binding to the surfaces. Conclusions: The impact of sugars and other factors on biofilm formation can vary depending on the type of bacteria present.