Journal
JOURNAL OF INTEGRATIVE AGRICULTURE
Volume 15, Issue 3, Pages 528-536Publisher
ELSEVIER SCI LTD
DOI: 10.1016/S2095-3119(15)61203-5
Keywords
non-heading Chinese cabbage; stamen-petalody; ethylene-responsive factor; gene expression
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Funding
- Independent Innovation Fund for Agricultural Science and Technology of Jiangsu Province, China [CX(15)1015]
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BrcERF-B3 gene, a member of ethylene-responsive factor family, was screened from a mutant plant in non-heading Chinese cabbage (Brassica rapa ssp. chinensis) by cDNA-AFLP technology. We got full length cDNA of two BrcERF-B3 genes by homology-based cloning from two materials and found that their nucleotide sequences were the same by sequencing. The BrcERF-B3 protein, belonging to the B3 subgroup of the ERF subfamily, shared a close relationship with B. rapa. RT-PCR result showed that BrcERF-B3 expressed only in mutant stamen rather than maintainer stamen. qRT-PCR results indicated that BrcERF-B3 expressed highly during reproductive growth development and in the early of mutant buds, suggesting BrcERF-B3 might be involved in the formation of abnormal flower in mutant. What's more, the expression of BrcERF-B3 was more significant to ABA, MeJA and cold stresses in mutant than in maintainer and was down-regulated in NaCl treatment in two lines, implying BrcERF-B3 might be different roles in biotic and abiotic stresses.
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