Article
Multidisciplinary Sciences
Sandrine Isaac, Alejandra Flor-Duro, Gloria Carruana, Leonor Puchades-Carrasco, Anna Quirant, Marina Lopez-Nogueroles, Antonio Pineda-Lucena, Marc Garcia-Garcera, Carles Ubeda
Summary: This study identifies nutrient depletion by specific commensal bacteria as a novel non-antibiotic strategy to reduce colonization of multidrug-resistant organisms (MDRO), particularly vancomycin-resistant Enterococcus (VRE), in the intestine.
NATURE COMMUNICATIONS
(2022)
Article
Agriculture, Dairy & Animal Science
Yanyan Zhang, Tao Wu, Zhenqiang Chen, Yuxuan Meng, Zhekun Zhu, Qian Wang, Junjie Tian, Dan Yi, Lei Wang, Di Zhao, Yongqing Hou
Summary: Dietary supplementation with E. faecium R1 improved intestinal function, decreased diarrhea incidence, and attenuated intestinal and liver injury in piglets challenged by lipopolysaccharide.
Article
Fisheries
Yanan Tian, Fang Wang, Xiaolei Su, Lele Zhang, Zhenhao Ma, Longkun Gao, Hansheng Yan, Yuanyuan Xue, Chunhui Lv, Xu Zhang, Mingzhu Li, Yuping Hou, Kai Wang
Summary: This study evaluated the probiotic effects of Enterococcus faecium supplementation on the growth, development, intestinal health, and immunity of big-belly seahorses during the diet conversion period. The concentration and frequency of E. faecium supplementation were found to significantly affect the growth performance of seahorses. Seahorses supplemented with high concentration of E. faecium showed improved growth performance and increased expression of intestinal immune-related genes. The remodeling of intestinal micro-biota and their functions also contributed to a healthier intestinal environment and increased resistance against pathogenic challenge.
AQUACULTURE REPORTS
(2023)
Article
Microbiology
Jin Zhou, Ji Luo, Shumin Yang, Qiling Xiao, Xiliang Wang, Zutao Zhou, Yuncai Xiao, Deshi Shi
Summary: This study found that HDRsEf1 has specific effects on the gut microbiota of weaned piglets, with significant changes observed in the ileum and cecum. Administration of HDRsEf1 significantly decreased Escherichia-Shigella in the ileum and increased butyrate-producing bacteria in the cecum, leading to improved intestinal morphological development and reduced inflammatory responses. The findings suggest that HDRsEf1 alters gut microbiota, thereby alleviating inflammation and promoting intestinal health in weaned piglets.
Article
Agriculture, Dairy & Animal Science
Jianping Wang, Chunpeng Wan, Zhao Shuju, Zengqiao Yang, Pietro Celi, Xuemei Ding, Shiping Bai, Qiufeng Zeng, Xiangbing Mao, Shengyu Xu, Keying Zhang, Mingxi Li
Summary: The study examined the difference in microbiota of breeders with different egg-laying rates, and found that breeders with higher egg-laying rates had better reproductive performance and microbiota diversity, while those with lower rates triggered energy storage leading to higher fat deposition.
Article
Immunology
Shimeng Huang, Xiaoping Rong, Meiling Liu, Zhongjun Liang, Yanqiang Geng, Xinyue Wang, Jianyun Zhang, Cheng Ji, Lihong Zhao, Qiugang Ma
Summary: This study investigated the effects of E. faecium on laying performance, egg quality, host metabolism, intestinal mucosal immunity, and gut microbiota of laying hens challenged with S. Enteritidis. The results showed that E. faecium supplementation improved egg production and shell thickness, regulated host lipid metabolism, enhanced immune functions, alleviated intestinal injuries, and improved gut microbial dysbiosis caused by S. Enteritidis infection.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Noha A. Ahmed, Rania Abdelmonem Khattab, Yasser M. Ragab, Mariam Hassan
Summary: This study evaluated the probiotic and safety characteristics of two E. lactis strains isolated from the human gut using in-vitro and in silico approaches. The results showed that the strains exhibited resistance to bile salts and displayed antibacterial activity against certain pathogens. Comparative genome analysis revealed the absence of transferable antibiotic resistance genes. These findings suggest that E. lactis could be a potential source for safer Enterococcus probiotic supplements.
Article
Nutrition & Dietetics
Lingshuang Yang, Xinqiang Xie, Ying Li, Lei Wu, Congcong Fan, Tingting Liang, Yu Xi, Shuanghong Yang, Haixin Li, Jumei Zhang, Yu Ding, Liang Xue, Moutong Chen, Juan Wang, Qingping Wu
Summary: The study found that Enterococcus faecium strain 132 and Lactobacillus paracasei strain 201 can effectively lower blood lipids, improve cholesterol metabolism, and reduce fat accumulation. Additionally, these two strains can alleviate symptoms of hypercholesterolemia by regulating gut microbiota and increasing levels of acetic acid and propionic acid in the intestines.
Article
Nutrition & Dietetics
Wenfeng Xu, Kaixiang Zou, Ying Zhan, Yunjie Cai, Zhihong Zhang, Xueying Tao, Liang Qiu, Hua Wei
Summary: This study identified a cholesterol-lowering strain, Enterococcus faecium GEFA01, from healthy lean individuals and evaluated its cholesterol-lowering capacity in mice fed a high-cholesterol and high-fat diet. E. faecium GEFA01 exhibited a high cholesterol removal rate and significantly decreased cholesterol levels in mice by modulation of gut microbiota short-chain fatty acid axis. The findings suggest that E. faecium GEFA01 has potential as a probiotic candidate for lowering cholesterol levels in the future.
FRONTIERS IN NUTRITION
(2022)
Article
Microbiology
Ge Huang, Yizheng Zhou, Hai Cheng, Tao Lv, Lisi Zheng, Chengbin Li, Yunbo Chen
Summary: Through genomic and transcriptomic analysis, we found differences in carbon metabolism and amino acids between endogenously infected and only-colonized Enterococcus faecium, suggesting metabolic diversity as a strategy for endogenous infection.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Md Rayhan Mahmud, Ching Jian, Md Karim Uddin, Mirja Huhtinen, Anne Salonen, Olli Peltoniemi, Heli Venhoranta, Claudio Oliviero
Summary: Small-scale studies on pigs' intestinal microbiota and growth performance have yielded inconsistent results. This study found that the gut microbiota during the nursery stage, not the suckling period, was a predictor of piglet growth. Certain SCFA-producing bacterial genera were significantly correlated with high piglet growth, and the gut microbiota of high-ADG piglets matured faster and stabilized sooner after weaning compared to low-ADG piglets. The findings suggest that weaning is a major driver of gut microbiota variation and that specific gut microbiota at weaning may be beneficial for piglet growth.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biotechnology & Applied Microbiology
Alexander Suvorov, Shuangzhi Zhao, Galina Leontieva, Galina Alekhina, Jinyu Yang, Anna Tsapieva, Alena Karaseva, Valentina Smelova, Danyang Guo, Leilei Chen
Summary: This study compared the probiotic effect of enterococcal Enterococcus faecium L3 to the antibiotic enramycin as a chicken feed additive. The results showed that chickens fed with the probiotic had greater weight gain, increased HDL levels in blood serum, and a different caecal microbiome composition compared to the control group. This suggests that probiotic E. faecium L3 could be a potential replacement for the antibiotic enramycin.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2023)
Article
Microbiology
Gregory S. Canfield, Anushila Chatterjee, Juliel Espinosa, Mihnea R. Mangalea, Emma K. Sheriff, Micah Keidan, Sara W. McBride, Bruce D. McCollister, Howard C. Hang, Breck A. Duerkop
Summary: Enterococcus faecium, a commensal of the human intestine, has evolved into a hospital-adapted, multidrug-resistant pathogen. Bacteriophages, natural predators of bacteria, show potential as therapeutics against MDR E. faecium infections, despite the unknown molecular events governing their interactions. While phage resistance may emerge, it is shown that lytic phages could effectively synergize with antibiotics to slow or inhibit E. faecium growth.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Biochemistry & Molecular Biology
Li Wang, Lin Qiao, Aike Li, Lixian Chen, Beibei He, Gang Liu, Weiwei Wang, Jun Fang
Summary: A high-temperature-resistant strain RS047-wl of Enterococcus faecium (E. faecium) was obtained through continuous heat-adaptation test. Multi-omics analysis revealed the involvement of 98 differentially expressed genes and 115 differential metabolites in regulating heat resistance, including the changes in AgrB, AgrC, and AgrA gene expressions in QS system pathways, accumulation of highly soluble osmotic substances, and down-regulation of organic acids metabolism and purine metabolism. These findings provide target genes for genetic modification and clues for screening heat-resistant bacteria to improve the heat resistance ability of E. faecium for production.
Article
Biochemistry & Molecular Biology
Yating Du, Shiqi Luo, Xin Zhou
Summary: This study presents the first complete genome sequence of Enterococcus faecium isolated from honey bee gut, and investigates the interactions between E. faecium and honey bees through transcriptome and miRNA analysis. It was found that colonization of E. faecium increased honey bee gut weight, with up-regulation of developmental genes and enrichment of down-regulated miRNAs in developmental pathways. The study adds insights on how E. faecium affects honey bee gut weight at both transcriptional and post-transcriptional levels, as well as the role of miRNAs in mediating host and bacteria interactions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Letter
Respiratory System
Yong Song, Siew-Kim Khoo, Khui Hung Lee, Mika Makela, Tari Haahtela, Peter LeSouef, Guicheng (Brad) Zhang
EUROPEAN RESPIRATORY JOURNAL
(2017)
Review
Allergy
Khui Hung Lee, Yong Song, Michael O'Sullivan, Gavin Pereira, Richard Loh, Guicheng (Brad) Zhang
INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY
(2017)
Article
Biotechnology & Applied Microbiology
Yang Wang, Wei Du, Kai Lei, Baikui Wang, Yuanyuan Wang, Yingshan Zhou, Weifen Li
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2017)
Article
Microbiology
W. Du, H. Xu, X. Mei, X. Cao, L. Gong, Y. Wu, Y. Li, D. Yu, S. Liu, Y. Wang, W. Li
BENEFICIAL MICROBES
(2018)
Article
Allergy
Ashlyn Poole, Yong Song, Michael O'Sullivan, Khui Hung Lee, Jessica Metcalfe, Jing Guo, Helen Brown, Ben Mullins, Richard Loh, Guicheng Brad Zhang
PEDIATRIC ALLERGY AND IMMUNOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Khui Hung Lee, Jing Guo, Yong Song, Amir Ariff, Michael O'Sullivan, Belinda Hales, Benjamin J. Mullins, Guicheng Zhang
Summary: This study identified Ruminococcaceae UCG-002 as potentially associated with IgE-mediated food allergy using 16S rRNA gene sequencing and network analysis. Functional pathway analysis revealed enrichment of methane metabolism and glycerolipid metabolism in the gut microbiome of food-allergic children.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Allergy
Khui Hung Lee, Anthony Bosco, Michael O'Sullivan, Yong Song, Jessica Metcalfe, Kan Yu, Benjamin J. Mullins, Richard Loh, Guicheng Zhang
Summary: This study revealed the changes in immune cells and gene network patterns in the pathophysiology of nut allergy. The results showed an increase in neutrophils, a decrease in CD4(+) T-cells and regulatory T-cells, and an upregulation of type 1 interferon signaling pathway associated with the development of nut allergy.
WORLD ALLERGY ORGANIZATION JOURNAL
(2022)
Review
Nursing
Weiting Liu, Carol Chunfeng Wang, Khui Hung Lee, Xiaopeng Ma, Timothy Leen Kang
Summary: This study suggests that acupuncture and moxibustion are more effective than the control group in managing primary dysmenorrhea (PD), providing effective pain relief with fewer adverse events.
CLINICAL NURSING RESEARCH
(2022)
Review
Integrative & Complementary Medicine
Weiting Liu, Carol Chunfeng Wang, Khui Hung Lee, Xiaopeng Ma, Timothy Leen Kang
Summary: Acupuncture and moxibustion have potential in managing primary dysmenorrhea, but there are methodological challenges in clinical studies, which require urgent attention. Randomized controlled trials with pragmatic designs may be more suitable for studying these therapies.
COMPLEMENTARY THERAPIES IN MEDICINE
(2022)
Review
Nursing
Binglei Chen, Carol Chunfeng Wang, Khui Hung Lee, Jianhong Cecilia Xia, Zongting Luo
Summary: This review aimed to synthesize the best available evidence on the efficacy and safety of acupuncture in managing depression. The analysis of 22 clinical trials found that electroacupuncture combined with antidepressants achieved superior outcomes, indicating that acupuncture, either in isolation or as an adjunct to pharmacological treatment, has clinical benefits and can be considered a safe option for managing depression.
RESEARCH IN NURSING & HEALTH
(2023)
Meeting Abstract
Cardiac & Cardiovascular Systems
K. Yu, K. H. Lee, E. Afrifa-Yamoah, J. Guo, N. Pachter, K. Harrison, J. Goldblatt, J. Xiao, G. Zhang