Article
Biochemistry & Molecular Biology
Chatvadee Kornsuthisopon, Sunisa Rochanavibhata, Nunthawan Nowwarote, Kevin A. Tompkins, Waleerat Sukarawan, Thanaphum Osathanon
Summary: The small molecule 6-bromoindirubin-3'-oxime (BIO) effectively modulates the osteogenic differentiation of hDPSCs by activating the Wnt signaling pathway. BIO promotes alkaline phosphatase activity and mineralization, while inhibiting colony formation, cell migration, and adipogenic differentiation. These findings suggest that BIO has potential as a bioactive molecule to enhance dentin repair.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Orthopedics
Xiao-hua Li, Fu-ling Chen, Hong-lin Shen
Summary: Salidroside enhanced the osteogenic differentiation of ADSCs through the Wnt/beta-catenin signaling pathway, as confirmed by increased cell viability, ALP activity, and calcium deposition, as well as upregulation of WNT3A and beta-catenin expression.
JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH
(2021)
Article
Developmental Biology
Sujie Hu, Sisi Chen, Haozu Zeng, Xinyi Ruan, Xinyi Lin, Rexhina Vlashi, Chenhe Zhou, Haidong Wang, Guiqian Chen
Summary: This study identified Ap-213 as a transcriptional factor with higher expression in frontal bone compared to parietal bone. It regulates osteoblast proliferation and differentiation through the Wnt/13-catenin signaling pathway. Ap-213 plays an important role in regulating regional osteogenic potential in frontal and parietal bone.
DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Jinwu Bai, Weijun Zhang, Chenwei Zhou, Guangfeng Zhao, Huiming Zhong, Kai Hang, Jianxiang Xu, Wei Zhang, Erman Chen, Jiaqi Wu, Ling Liu, Deting Xue
Summary: MFG-E8 promotes osteogenic differentiation of BMSCs by regulating the GSK3 beta/beta-catenin signaling pathway and therefore, is a potential therapeutic target. This study has important clinical significance in addressing fracture nonunion and bone defects.
Article
Orthopedics
Wei Guo, Xiao-guang Yang, Yu-lin Shi, Hong Wang
Summary: The study found that paeoniflorin can promote osteogenic differentiation of MC3T3-E1 cells by regulating the Wnt/beta-catenin pathway. This has potential implications for the treatment of osteoporosis.
JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH
(2022)
Article
Cell & Tissue Engineering
Youde Liang, Xin Liu, Ruiping Zhou, Dawei Song, Yi-Zhou Jiang, Weiwei Xue
Summary: Mesenchymal stem cells (MSCs) combined with Chaetocin show enhanced osteogenic ability, reduced adipogenic ability, and improved Wnt/β-catenin signaling pathway activity, leading to promotion of bone tissue regeneration and repair.
STEM CELLS INTERNATIONAL
(2021)
Article
Cell & Tissue Engineering
Maorui Zhang, Yujin Gao, Qing Li, Huayue Cao, Jianghua Yang, Xiaoxiao Cai, Jingang Xiao
Summary: This study investigates the impact of the diabetic environment on the osteogenic potential of adipose-derived stem cells (ASCs) and explores the role of DNA methylation and the Wnt/beta-catenin signaling pathway. The findings suggest that downregulating Dnmt3a rescues the poor osteogenic potential of ASCs in diabetic osteoporosis and provides a possible approach for bone tissue regeneration in these patients.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Nanoscience & Nanotechnology
Yibo Zhang, Yawen Li, Wei Liao, Wenzao Peng, Jianghui Qin, Dongyang Chen, Liming Zheng, Wenjin Yan, Lan Li, Zhirui Guo, Peng Wang, Qing Jiang
Summary: The study demonstrated that citrate-stabilized gold nanorods have good biocompatibility and can promote osteogenic differentiation of multiple cell types through the Wnt/beta-catenin signaling pathway.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2021)
Article
Cell & Tissue Engineering
Xiaowen Wu, Xiaochen Wang, Liying Shan, Jie Zhou, Xin Zhang, Endong Zhu, Hairui Yuan, Baoli Wang
Summary: The loss of NFIX gene affects endochondral ossification and mineralization in bone, with NFIX inducing osteoblast differentiation and impairing adipocyte formation by regulating HMGA1 expression. HMGA1 plays a role in osteoblast commitment and mediates NFIX function by activating canonical Wnt signaling pathways.
Article
Cell & Tissue Engineering
Hongwei Gao, Hui Dong, Jiachun Zheng, Xufeng Jiang, Mingzhi Gong, Le Hu, Jinshan He, Yongxiang Wang
Summary: The study identified LINC01119 as a negative regulator of osteogenic differentiation in MSCs, with its knockdown promoting calcification and osteogenic differentiation. It was revealed that LINC01119 negatively regulates osteogenesis through the Wnt pathway by targeting FZD4, with transcription factor EBF3 directly binding to LINC01119's promoter region.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Cell Biology
Jiaqi Guo, Linglin Qian, Jingjing Ji, Zhenjun Ji, Yu Jiang, Ya Wu, Ziwei Yang, Genshan Ma, Yuyu Yao
Summary: Serpina3c promotes adipogenesis and maintains normal fat function by inhibiting the Wnt/beta-catenin pathway. Serpina3c knockout mice exhibit reduced body weight and white adipose tissue weight, and increased lipid depositions in the liver and muscles. In addition, knockout mice show severe insulin resistance.
CELLULAR SIGNALLING
(2022)
Article
Biochemistry & Molecular Biology
Haoran Li, Wuling Zhou, Shiwei Sun, Tianlong Zhang, Tieqi Zhang, Haitian Huang, Minghai Wang
Summary: This study found that MFAP5 regulates osteogenesis in BMSCs through the Wnt/beta-catenin and AMPK signaling pathways, and may serve as a therapeutic target for patients with osteoporosis.
MOLECULAR MEDICINE
(2021)
Article
Dentistry, Oral Surgery & Medicine
Ming Ying, Bin Zhang
Summary: This study investigated the effect of daidzein on the proliferation and osteogenic differentiation of human periodontal ligament stem cells. It was found that daidzein promotes these processes by activating the Wnt/β-catenin signaling pathway.
Article
Chemistry, Applied
Na Ren, Xin Yu, Aizhu Wang, Na Liang, Zhichao Feng, Chunhui Sun
Summary: The study demonstrates that ScCl3 can enhance proliferation and osteogenic differentiation of rat bone MSCs while reducing adipogenic differentiation to a certain extent through the Wnt/beta-catenin signaling pathway. Appropriate concentrations of ScCl3 show potential in improving bone remodeling and may lead to new strategies for designing composites for biomedical applications in the future.
JOURNAL OF RARE EARTHS
(2022)
Article
Cell Biology
Yuexin Xu, Xiaogang Bao, Xiaoyun Chen, Peixuan Wu, Shiyu Chen, Bowen Zhang, Jing Ma, Guohua Xu, Duan Ma
Summary: This study revealed that STARD3NL suppressed osteogenesis by binding with Annexin A2, resulting in the inactivation of Wnt signaling. Inhibition of Stard3nl could reverse bone loss in osteoporotic mice.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2022)
Article
Cardiac & Cardiovascular Systems
Jarmon G. Lees, Marek Napierala, Alice Pebay, Mirella Dottori, Shiang Y. Lim
Summary: Friedreich's ataxia (FRDA) is a hereditary neuromuscular disorder with cardiomyopathy being the main cause of premature death. The cardiomyopathy associated with FRDA is a complex and progressive disease without effective treatment options, involving various pathological mechanisms at the cellular level such as cardiomyocyte hypertrophy, apoptosis, and fibrosis, as well as the participation of non-myocyte cells like vascular cells and inflammatory cells.
INTERNATIONAL JOURNAL OF CARDIOLOGY
(2022)
Article
Plant Sciences
Pieter-Willem Hendriks, Peter R. Ryan, Philip Hands, Vivien Rolland, Saliya Gurusinghe, Leslie A. Weston, Greg J. Rebetzke, Emmanuel Delhaize
Summary: The selection for early shoot vigour in wheat is associated with increased root hair length and changes to cell size in roots and shoots. Six cycles of recurrent selection for early shoot vigour in wheat resulted in significant increases in leaf width and shoot biomass. Increased shoot vigour was associated with greater root biomass, larger rhizosheath size, and longer root hairs.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Agronomy
Saliya Gurusinghe, K. M. Shamsul Haque, Paul A. Weston, William B. Brown, Leslie A. Weston
Summary: The use of competitive crops in successional rotations has been shown to reduce the growth and establishment of annual weeds by depleting the weed seedbank. Rotational treatments, such as dual-purpose cereals or a canola break-crop along with cereals, were effective in suppressing weed propagule numbers. Rotational selection is an important and cost-effective tool in integrated weed management systems when applied over multiple growing seasons.
Article
Agronomy
Pieter-Willem Hendriks, Saliya Gurusinghe, Peter R. Ryan, Gregory J. Rebetzke, Leslie A. Weston
Summary: This study evaluated the impact of increased early shoot vigour on the weed competitiveness of Australian wheats and found that enhancing wheat competitiveness through early vigour can significantly suppress weed growth.
Review
Cardiac & Cardiovascular Systems
Ren Jie Phang, Rebecca H. Ritchie, Derek J. Hausenloy, Jarmon G. Lees, Shiang Y. Lim
Summary: Patients with Type 2 diabetes often suffer from a distinct cardiac condition called diabetic cardiomyopathy, which is not well understood and has limited treatment options. This review focuses on the roles of non-myocyte cells in the heart and the potential use of sodium-glucose cotransporter 2 inhibitors as a therapy for diabetic cardiomyopathy.
CARDIOVASCULAR RESEARCH
(2023)
Review
Oncology
Yali Deng, Doan T. M. Ngo, Jessica K. Holien, Jarmon G. Lees, Shiang Y. Lim
Summary: Drp1 inhibition holds great potential as a targeted mitochondrial therapy for doxorubicin-induced cardiotoxicity, which is a significant challenge in clinical practice.
CURRENT ONCOLOGY REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Jonathan Lozano, Alin Rai, Jarmon G. Lees, Haoyun Fang, Bethany Claridge, Shiang Y. Lim, David W. Greening
Summary: This study presents a rapid method to generate large quantities of EV-like nanovesicles from human iPSCs, which can regulate cardiac cells and have therapeutic potential. The nanovesicles carry proteins similar to the parental cells and promote angiogenesis and cardiomyocyte survival while attenuating fibroblast activation in the heart.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Quanxia Lyu, Shu Gong, Jarmon G. Lees, Jialiang Yin, Lim Wei Yap, Anne M. Kong, Qianqian Shi, Runfang Fu, Qiang Zhu, Ash Dyer, Jennifer M. Dyson, Shiang Y. Lim, Wenlong Cheng
Summary: This study presents a method using a soft resistive force-sensing diaphragm to monitor the contraction forces and beating patterns of cardiac organoids in real-time. The method overcomes the mismatch between the topological complexity and soft tissue properties of organoids and rigid force sensors, providing a new approach for studying the mechanical properties of cardiac tissues.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Ayeshah A. Rosdah, Belinda M. Abbott, Christopher G. Langendorf, Yali Deng, Jia Q. Truong, Helen M. M. Waddell, Naomi X. Y. Ling, William J. Smiles, Lea M. D. Delbridge, Guei-Sheung Liu, Jonathan S. Oakhill, Shiang Y. Lim, Jessica K. Holien
Summary: Mitochondrial dynamin-related protein 1 (Drp1) is a key regulator of mitochondrial dynamics. This study identified a novel small molecule inhibitor, DRP1i27, that specifically binds to human Drp1 isoform 3 and enhances cellular networks of fused mitochondria.
SCIENTIFIC REPORTS
(2022)
Article
Agronomy
Long Ma, Nirodha Weeraratne, Saliya Gurusinghe, Jesmin Aktar, K. M. Shamsul Haque, Philip Eberbach, Geoff G. Gurr, Leslie A. Weston
Summary: The introduction of exotic dung beetles in southern Australia has reduced the amount of livestock dung on the soil surface, but its impact on pasture quality and soil nutrient mobility has not been studied in the Riverina region of New South Wales. This study evaluated the effects of a common introduced dung beetle (Bubas bison) on water quality and soil microbiome using lysimetry and DNA assessment. The results showed that dung beetles and soil type influenced pasture performance and microbial communities, although water quality seemed unaffected.
Article
Biochemistry & Molecular Biology
Jiang-Hui Wang, Daniel Urrutia-Cabrera, Jarmon G. Lees, Santiago Mesa Mora, Tu Nguyen, Sandy S. C. Hung, Alex W. Hewitt, Shiang Y. Lim, Thomas L. Edwards, Raymond C. B. Wong
Summary: Age-related macular degeneration (AMD) is a blinding disease characterized by dysfunction of the retinal pigmented epithelium (RPE). This study developed a human RPE model using CRISPRi technology, providing a useful tool for functional studies of AMD-associated genes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Agronomy
Jeffrey D. D. Weidenhamer, Don Cipollini, Kathryn Morris, Saliya Gurusinghe, Leslie A. A. Weston
Summary: Progress in understanding allelopathic interactions among plants has been limited by the complexity of direct and indirect interactions, as well as the influence of various factors such as resource limitations, pathogens, and herbivores. Recent advancements in technology and analytical techniques have allowed researchers to study the biosynthesis of allelochemicals and plant responses in more detail. This review highlights the importance of applying these new approaches in ecologically rigorous ways to enhance our understanding of allelopathic interactions.
Review
Agronomy
Thomas Nordblom, Saliya Gurusinghe, Andrew Erbacher, Leslie A. Weston
Summary: Southern Australian farming systems face limitations in choosing cover crops suitable for various purposes due to Mediterranean climatic conditions. Recent studies have shown the potential benefits of cover crop inclusion in viticulture and pasture systems with lower moisture stress. However, the delivery of cover crop benefits is greatly influenced by soil type and regional climatic conditions. Therefore, longer-term field evaluations and investigations of termination options under varying environmental and soil conditions are needed.
Article
Entomology
Nisansala N. Perera, Russell A. Barrow, Paul A. Weston, Vivien Rolland, Philip Hands, Saliya Gurusinghe, Leslie A. Weston, Geoff M. Gurr
Summary: This study investigated the structure and distribution of antennal sensilla of three introduced dung beetle species in Australia using scanning electron microscopy, and examined their olfactory responses to different dung volatiles using electroantennography. The results revealed species-specific olfactory responses of dung beetles to volatiles, which has important implications for attracting dung beetles in the field.
Article
Cell Biology
Tu Nguyen, Daniel Urrutia-Cabrera, Luozixian Wang, Jarmon G. Lees, Jiang-Hui Wang, Sandy S. C. Hung, Alex W. Hewitt, Thomas L. Edwards, Sam McLenachan, Fred K. Chen, Shiang Y. Lim, Chi D. Luu, Robyn Guymer, Raymond C. B. Wong
Summary: This study generated a POLDIP2 knockout cell line in human retinal pigment epithelium cells using CRISPR/Cas technology. It found a link between POLDIP2 and age-related macular degeneration (AMD), and showed that the knockout of POLDIP2 does not affect cell proliferation, cell viability, phagocytosis, and autophagy. RNA sequencing revealed significant changes in genes related to immune response, complement activation, oxidative damage, and vascular development in the POLDIP2 knockout cells. Additionally, the study demonstrated that loss of POLDIP2 reduces mitochondrial superoxide levels and upregulates mitochondrial superoxide dismutase SOD2 in ARPE-19 cells, supporting a potential role of POLDIP2 in regulating oxidative stress in AMD pathology.