Article
Cell & Tissue Engineering
Pegah Nammian, Seyedeh-Leili Asadi-Yousefabad, Sajad Daneshi, Mohammad Hasan Sheikhha, Seyed Mohammad Bagher Tabei, Vahid Razban
Summary: The study compared the effects of allogeneic bone marrow mesenchymal stem cells (BM-MSCs) and adipose tissue mesenchymal stem cells (AT-MSCs) in the treatment of critical limb ischemia (CLI). The results indicated that BM-MSCs had a stronger effect on endothelial cell migration, muscle restructure, functional improvements, and neovascularization in ischemic tissues compared to AT-MSCs. This suggests that BM-MSC transplantation could be a more effective therapy for diseases with insufficient angiogenesis, such as hindlimb ischemia.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Cardiac & Cardiovascular Systems
Lirong Xu, Yutong Liu, Qianyun Cheng, Yang Shen, Ye Yuan, Xiaolang Jiang, Xu Li, Daqiao Guo, Junhao Jiang, Changpo Lin
Summary: CLI, the most advanced clinical stage of peripheral vascular disease, leads to lower extremity rest pain, ulceration, and gangrene due to insufficient blood and oxygen supply, making effective biomarkers and therapeutic targets crucial. The study confirmed the downregulation of circadian gene Bmal1 in CLI patients, affecting inflammation and angiogenesis. Bmal1 disruption impairs angiogenesis and endothelial cell functions, suggesting its potential as a biomarker and therapeutic target in CLI.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2021)
Article
Cardiac & Cardiovascular Systems
Lirong Xu, Yutong Liu, Qianyun Cheng, Yang Shen, Ye Yuan, Xiaolang Jiang, Xu Li, Daqiao Guo, Junhao Jiang, Changpo Lin
Summary: CLI, the most advanced stage of peripheral vascular disease, involves downregulation of circadian gene Bmal1 which affects lipid metabolism, inflammation, and angiogenesis. This downregulation impairs angiogenesis and endothelial cell functions, while repressing inflammation and promoting angiogenesis through transcriptional regulation of VEGF. Therefore, Bmal1 may serve as an effective biomarker and therapeutic target in CLI.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2021)
Review
Cell & Tissue Engineering
Zeinab Shirbaghaee, Mohammad Hassani, Saeed Heidari Keshel, Masoud Soleimani
Summary: This article reviews the immunomodulatory features of mesenchymal stem cells (MSCs) and their potential applications as therapeutic agents in the treatment of critical limb ischemia (CLI). Despite advances in clinical research, MSC therapy is still considered experimental and not recommended for regular clinical use. The article provides recommendations to improve the efficacy of cell therapy for CLI patients.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Medicine, General & Internal
Alessia Scatena, Pasquale Petruzzi, Filippo Maioli, Francesca Lucaroni, Cristina Ambrosone, Giorgio Ventoruzzo, Francesco Liistro, Danilo Tacconi, Marianna Di Filippi, Nico Attempati, Leonardo Palombi, Leonardo Ercolini, Leonardo Bolognese
Summary: The study showed that treatment with autologous PBMNC implants in NO-CLI patients with DFUs significantly reduced amputation rate, improved survival, and promoted wound healing. Autologous PBMNC injection may effectively prevent amputations in patients with DFUs.
JOURNAL OF CLINICAL MEDICINE
(2021)
Review
Cell & Tissue Engineering
Laura Lozano Navarro, Xueyi Chen, Lady Tatiana Girata Viviescas, Andrea K. Ardila-Roa, Maria L. Luna-Gonzalez, Claudia L. Sossa, Martha L. Arango-Rodriguez
Summary: Peripheral arterial disease affects hundreds of millions of people worldwide and can lead to limb amputation or death. Revascularization is crucial for preventing amputation, but a significant portion of patients are not suitable for traditional surgical treatment, resulting in higher amputation rates. Mesenchymal stem cells have emerged as a promising cell-based therapy for peripheral arterial disease due to their regenerative properties.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Pharmacology & Pharmacy
Tao Chen, Bo Ye, Jing Tan, Haifeng Yang, Faming He, Raouf A. Khalil
Summary: Mesenchymal stem cells, especially those expressing CD146, show potential in improving vascularity and muscle function in a rat model of hind-limb ischemia, reducing apoptosis and increasing vascular endothelial growth factor expression.
BIOCHEMICAL PHARMACOLOGY
(2021)
Article
Surgery
Robert Myers, Bakhtawar Mushtaq, Nicholas Taylor, Hajar Rashid, Danielle M. Pineda
Summary: This study aims to understand the impact of bypass surgery on limb salvage in patients aged >80 years with critical limb ischemia. The study found that there was no significant difference in limb salvage, hospital length of stay, and primary patency between patients aged <80 years and those aged >=80 years. However, further studies are needed to determine the statistical impact on mortality in this population with a larger cohort.
JOURNAL OF VASCULAR SURGERY
(2023)
Article
Peripheral Vascular Disease
Michael G. McCoy, Anurag Jamaiyar, Grasiele Sausen, Henry S. Cheng, Daniel Perez-Cremades, Rulin Zhuang, Jingshu Chen, Philip P. Goodney, Mark A. Creager, Marc S. Sabatine, Marc P. Bonaca, Mark W. Feinberg
Summary: Peripheral artery disease (PAD) is a serious condition that can lead to limb loss and death, especially in subjects with diabetes. In this study, researchers identified miR-375 as a potential therapeutic target for critical limb ischemia (CLI), an advanced form of PAD. They found that miR-375, when overexpressed, promotes angiogenesis and arteriogenesis, improving blood flow recovery in diabetic mice. Further investigation revealed that miR-375 regulates the NF-kB signaling pathway by targeting Kruppel-like factor 5 (KLF5). These findings suggest that targeting the miR-375-KLF5-NF-kB signaling axis may be an effective strategy for treating CLI in diabetes.
Review
Engineering, Biomedical
Zheng Xing, Chen Zhao, Siwen Wu, Chunchen Zhang, Haifeng Liu, Yubo Fan
Summary: Therapeutic angiogenesis provides a potential solution for critical limb ischemia by manipulating blood vessel growth in vivo without the need for extra pharmaceutics and surgery. With the rise of interdisciplinary research, regenerative medicine strategies offer new possibilities for treating various clinical diseases.
Article
Cell & Tissue Engineering
Christy Wing Tung Wong, Apurva Sawhney, Yalan Wu, Yi Wah Mak, Xiao Yu Tian, Hon Fai Chan, Anna Blocki
Summary: This study demonstrated that functional BDACs can be sourced under xeno- and/or serum-free conditions and showed superior therapeutic effects compared to traditional MSCs in a murine model of CLI. The secretome of BDACs contained a larger number of proteins with pro-angiogenic and anti-inflammatory properties, potentially contributing to their enhanced therapeutic potential.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Cell Biology
Yipeng Du, Xiaoting Li, Wenying Yan, Zhaohua Zeng, Dunzheng Han, Hong Ouyang, Xiudi Pan, Bihui Luo, Bohua Zhou, Qiang Fu, Dongfeng Lu, Zheng Huang, Zhiliang Li
Summary: The study utilized mutant MetRS to label proteomes of mesenchymal stem cells and demonstrated efficient incorporation of ANL in protein synthesis in ischemic hind limb. The transplanted MSCs significantly improved blood reperfusion and vessel density while reducing inflammation in hindlimb ischemia model. Proteomic analysis revealed differential protein expression in apoptosis and energy metabolism pathways between ischemic and sham groups.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Lucia Beltran-Camacho, Marta Rojas-Torres, Ma Carmen Duran-Ruiz
Summary: Critical limb ischemia is the most severe form of peripheral arterial disease, commonly caused by atherosclerosis leading to progressive blockage of arterial vessels. For patients not suitable for surgical intervention, angiogenic cell therapy has emerged as a promising alternative, showing potential for enhancing revascularization by promoting vessel formation and blood flow recovery.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Yu-lin Cao, Wen-lan Chen, Qian Lei, Fei Gao, Wen-xiang Ren, Li Chen, Hong-xiang Wang, Ting Chen, Qiu-bai Li, Zhi-chao Chen
Summary: The study found that rapamycin can reverse the proangiogenic activity of senescent human umbilical cord mesenchymal stromal/stem cells (MSCs), which is important for treating ischemic diseases and tissue engineering.
ACTA PHARMACOLOGICA SINICA
(2022)
Article
Cardiac & Cardiovascular Systems
Xiaolang Jiang, Hao Liu, Tianyue Pan, Shiyang Gu, Yuan Fang, Zheng Wei, Gang Fang, Bin Chen, Junhao Jiang, Yun Shi, Peng Liu, Weiguo Fu, Zhihui Dong
Summary: The study demonstrated that using peripheral blood mononuclear cells for AICLTI treatment has favorable long-term outcomes, improving patients' quality of life, restoring labor competence, and significantly reducing major amputation rates.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2021)
Article
Peripheral Vascular Disease
Rupert Bauersachs, Marianne Brodmann, Christopher Clark, Sebastian Debus, Marco De Carlo, Jorge Francisco Gomez-Cerezo, Juraj Madaric, Lucia Mazzolai, Jean-Baptiste Ricco, Henrik Sillesen, Victor Aboyans
Summary: Public awareness of PAD is generally low internationally, despite its commonality. There are disparities in PAD familiarity levels between countries and age groups. Awareness campaigns are needed to improve early diagnosis and encourage the management of PAD risk factors.
VASA-EUROPEAN JOURNAL OF VASCULAR MEDICINE
(2021)
Article
Medicine, General & Internal
Gualtiero Palareti, Angelo Bignamini, Michela Cini, Young-Jun Li, Tomasz Urbanek, Juraj Madaric, Kamel Bouslama, German Y. Sokurenko, Giuseppe M. Andreozzi, Jiri Matuska, Armando Mansilha, Victor Barinov
Summary: The study analyzed the treatment decisions for VTE patients in 7 countries, finding differences in management strategies. Most patients used DOACs, with an average maintenance anticoagulation duration of around 6 months. Approximately 20% stopped anticoagulation, 50% extended it, and over 25% received antithrombotic drugs instead of anticoagulants for extended treatment.
INTERNAL AND EMERGENCY MEDICINE
(2022)
Article
Biology
Klara Cverenkarova, Martina Valachovicova, Tomas Mackulak, Lukas Zemlicka, Lucia Birosova
Summary: Microplastics have become a widespread environmental contaminant, endangering animal life and the food chain, requiring more attention for further research and protection of public health.
Editorial Material
Peripheral Vascular Disease
Andrej Klepanec, Matus Hoferica, Jan Harsany, Guerkan Sengoelge
Summary: This article introduces the application of Surfacer(R)Inside-out system in the treatment of chronic thoracic venous occlusion (CTVO), and reports the first case to our knowledge of successful restoration of dialysis access using the system via an unconventional and transcollateral approach.
JOURNAL OF VASCULAR ACCESS
(2022)
Article
Hematology
Gualtiero Palareti, Angelo A. Bignamini, Tomasz Urbanek, Michela Cini, Young-Jun Li, Juraj Madaric, Kamel Bouslama, German Y. Sokurenko, Giuseppe M. Andreozzi, Jiri Matuska, Armando Mansilha, Victor Barinov
Summary: This study investigated the influence of clinical presentation, extent of venous thrombosis, and presence of residual vein obstruction on the decision about the duration of secondary prophylaxis after a first venous thromboembolism. The results showed that the decision varied among countries, with most patients with proximal or associated deep vein thrombosis continuing treatment, while patients with isolated distal thrombosis were more likely to stop treatment. Factors such as presence of concomitant diseases and risk of post-thrombotic syndrome also influenced the decision.
THROMBOSIS RESEARCH
(2022)
Article
Environmental Sciences
Dusan Salat, Denisa Nikodemova, Andrej Klepanec, Viera Lehotska, Anna Salatova
Summary: Optimization in mammography is crucial for achieving a balance between image quality and patient dose. This study analyzed DRL compliance in 16 mammography screening centers and proposed new DRLs for screening mammography centers in Slovakia. The new DRLs were significantly lower than the existing national DRLs and the achievable AGD levels recommended in the European Guidelines for Quality Assurance in Breast Cancer Screening and Diagnosis.
RADIATION PROTECTION DOSIMETRY
(2022)
Article
Chemistry, Medicinal
Tomas Jasenovec, Dominika Radosinska, Marta Kollarova, Norbert Vrbjar, Peter Balis, Simona Trubacova, Ludovit Paulis, Lubomira Tothova, Ivana Shawkatova, Jana Radosinska
Summary: The objective of this study was to characterize monocrotaline-induced pulmonary arterial hypertension in a rat model and analyze the effects of bosentan. The study confirmed the effects of monocrotaline on lungs, heart, and pulmonary arterial pressure. Activation of the alternative pathway of the renin-angiotensin system and modifications of erythrocyte Na,K-ATPase enzyme kinetics were observed. Bosentan exhibited antioxidant effects and beneficial hemodynamic effects in PAH condition.
Article
Medicine, General & Internal
Martin Bendszus, Jens Fiehler, Fabien Subtil, Susanne Bonekamp, Anne Hege Aamodt, Blanca Fuentes, Elke R. Gizewski, Michael D. Hill, Antonin Krajina, Laurent Pierot, Claus Z. Simonsen, Kamil Zelenak, Rolf A. Blauenfeldt, Bastian Cheng, Angelique Denis, Hannes Deutschmann, Franziska Dorn, Fabian Flottmann, Susanne Gellissen, Johannes C. Gerber, Mayank Goyal, Jozef Haring, Christian Herweh, Silke Hopf-Jensen, Vi Tuan Hua, Maerit Jensen, Andreas Kastrup, Christiane Fee Keil, Andrej Klepanec, Egon Kurca, Ronni Mikkelsen, Markus Moehlenbruch, Stefan Mueller-Huelsbeck, Nico Muennich, Paolo Pagano, Panagiotis Papanagiotou, Gabor C. Petzold, Mirko Pham, Volker Puetz, Jan Raupach, Gernot Reimann, Peter Arthur Ringleb, Maximilian Schell, Eckhard Schlemm, Silvia Schoenenberger, Bjorn Tennoe, Christian Ulfert, Katerina Valis, Eva Vitkova, Dominik F. Vollherbst, Wolfgang Wick, Goetz Thomalla
Summary: Endovascular thrombectomy is associated with improved functional outcome and lower mortality in patients with acute ischemic stroke from large vessel occlusion using non-contrast CT as the primary imaging modality for patient selection.
Article
Multidisciplinary Sciences
Dominika Kochanova, Sachin Gulati, Matus Durdik, Lukas Jakl, Pavol Kosik, Milan Skorvaga, Katarina Vrobelova, Katarina Vigasova, Eva Markova, Dusan Salat, Andrej Klepanec, Igor Belyaev
Summary: The present study evaluated the genomic instability in interventional radiologists and found that long-term exposure to low-dose ionizing radiation increased the frequency of micronuclei and various types of chromosomal aberrations in these medical professionals.
SCIENTIFIC REPORTS
(2023)
Meeting Abstract
Physiology
T. Jasenovec, D. Radosinska, M. Kollarova, N. Vrbjar, P. Balis, S. Trubacova, L. Paulis, L. Tothova, J. Radosinska
Article
Medicine, General & Internal
Marian Ondrejkovic, Dusan Salat, Daniel Cambal, Andrej Klepanec
Summary: This study aimed to evaluate the impact of high and irregular heart rate on the image quality and radiation exposure in CT coronary angiography using a 256-row, 16cm wide detector CT system. The results showed that patients with a regular heart rate had better image quality and lower radiation dose compared to patients with an irregular heart rhythm. The use of a CT scanner with a 16cm wide detector enabled low radiation exposure even at high heart rates or irregular heart rhythms.
Article
Biochemistry & Molecular Biology
Katarina Grossmannova, Monika Barathova, Petra Belvoncikova, Viliam Lauko, Lucia Csaderova, Jan Tomka, Tomas Dulka, Jaromir Pastorek, Juraj Madaric
Summary: Abdominal aortic aneurysms (AAA) are a significant cause of premature deaths worldwide. In this study, the presence of hypoxia-related protein CA IX in AAA tissues was confirmed, suggesting its potential role in aneurysmal wall remodeling.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Meeting Abstract
Peripheral Vascular Disease
Benjamin Vigl, Simona Trubacova, Marko Poglitsch, Oliver Domenig, Peter Balis, Andrej Barta, Pavol Valovic, Silvia Liskova, Fedor Simko, Ludovit Paulis
JOURNAL OF HYPERTENSION
(2021)
Meeting Abstract
Peripheral Vascular Disease
Zuzana Kmecova, Jana Veteskova, Katarina Zirova, Lenka Bies Pivackova, Gabriel Doka, Eva Malikova, Peter Krenek, Nina Passakova, Ludovit Paulis, Milan Luknar, Eva Goncalvesova, Jan Klimas
JOURNAL OF HYPERTENSION
(2021)
Article
Chemistry, Multidisciplinary
Martina Valachovicova, Jana Pribojova, Csilla Mislanova
Summary: In Slovakia, about 200 kidney transplants are performed annually, with each patient requiring personalized immunosuppressive treatment to prevent rejection. A validated high-performance liquid chromatography connected to tandem mass spectrometry method was used to determine specific drugs in whole blood, providing reliable and reproducible results suitable for routine clinical practice.