Article
Microbiology
Dandan Fu, Qianqian Zheng, Xiaoyan Wu, Jianmei Wu, Ying Shao, Zhenyu Wang, Jian Tu, Xiangjun Song, Kezong Qi
Summary: Avian pathogenic Escherichia coli (APEC) causes avian colibacillosis in poultry. In this study, it was found that regulator EtrA positively regulates the expression of OmpW, and both EtrA and OmpW contribute to the motility, biofilm formation, serum resistance, and pathogenicity of APEC.
VETERINARY MICROBIOLOGY
(2023)
Article
Immunology
Dandan Fu, Jiaxuan Li, Jianmei Wu, Ying Shao, Xiangjun Song, Jian Tu, Kezong Qi
Summary: The transcriptional regulator EivF, part of the ETT2 cluster in E. coli strains, plays a crucial role in the pathogenicity of APEC by regulating genes related to membrane, adhesion, environmental stress, and secretion proteins. Its inactivation enhances resistance to serum, adherence to chicken cells, and colonization ability in chicks, indicating its negative repressor role in APEC pathogenicity.
MICROBIAL PATHOGENESIS
(2021)
Article
Agriculture, Dairy & Animal Science
Xiangkun Wang, Lingzi Li, Hongqi Shang, Fan Zhou, Cheng Wang, Shuyu Zhang, Panpan Gao, Ping Guo, Ruiliang Zhu, Zhenhong Sun, Kai Wei
Summary: This study found that duck circovirus infection in the duck population in China can cause immunosuppression and secondary infection. The virus affects the development of immune organs in infected ducks and increases the pathogenicity and colonization ability of Avian Pathogenic Escherichia coli (APEC).
Article
Agriculture, Dairy & Animal Science
Jian Tu, Dandan Fu, Yi Gu, Ying Shao, Xiangjun Song, Mei Xue, Kezong Qi
Summary: APEC is the responsible pathogen for colibacillosis in poultry, and the role of YgeK in APEC has not been reported. This study found that YgeK plays a crucial role in APEC virulence by affecting flagellar formation, bacterial motility, serum sensitivity, and adhesion ability. The results contribute to understanding the function of the ETT2 cluster in APEC and clarify its pathogenic mechanism.
Article
Veterinary Sciences
Dandan Fu, Ying Shao, Jiaxuan Li, Jianmei Wu, Xiaoyan Wu, Xiangjun Song, Jian Tu, Kezong Qi
Summary: The transcription regulator YjjQ plays an important role in APEC biofilm formation and motility by affecting the expression of relevant genes. The findings of this study provide new insights into the pathogenicity of APEC and have implications for its prevention and control.
RESEARCH IN VETERINARY SCIENCE
(2022)
Article
Multidisciplinary Sciences
Darby M. Newman, Nicolle L. Barbieri, Aline L. de Oliveira, Dajour Willis, Lisa K. Nolan, Catherine M. Logue
Summary: Colibacillosis caused by avian pathogenic Escherichia coli (APEC) is a devastating disease in poultry industry, causing significant economic losses. Study found that APEC strains commonly carry virulence-associated genes and ColV plasmid genes, form biofilms, and exhibit antimicrobial resistance.
Article
Agriculture, Dairy & Animal Science
Kai Ma, Hui Wang, Zhenfei Lv, Yutong Hu, Hongli Wang, Fang Shu, Chengfeng Zhu, Ting Xue
Summary: This study investigated the role of the two-component system CpxRA in APEC and found that it is involved in bacterial biofilm formation, antibiotic susceptibility, and transcription levels of the efflux pump emrKY. The results confirm the important regulatory role of the CpxRA two-component system in APEC resistance under antibiotic stress.
Review
Microbiology
Adrianna Aleksandrowicz, Muhammad Moman Khan, Katarzyna Sidorczuk, Mateusz Noszka, Rafal Kolenda
Summary: APEC, a common pathogen in poultry, relies on various adhesins to evade host defense mechanisms and cause infection. Understanding these adhesins is essential for developing effective strategies to combat APEC infections.
VETERINARY MICROBIOLOGY
(2021)
Article
Microbiology
Marianne Nicolas, Angelina Trotereau, Antoine Culot, Arshnee Moodley, Robert Atterbury, Jeroen Wagemans, Rob Lavigne, Philippe Velge, Catherine Schouler
Summary: Colibacillosis, the most common bacterial disease affecting poultry, is primarily treated with antibiotics. However, the rise of multidrug-resistant avian-pathogenic Escherichia coli (APEC) necessitates the exploration of alternative treatments, such as phage therapy. This study isolates and characterizes 19 genetically diverse coliphages, and demonstrates their efficacy in controlling APEC infections in ovo.
MICROBIOLOGY SPECTRUM
(2023)
Article
Food Science & Technology
Peili Wang, Jiaxiang Zhang, Yanfei Chen, Haoran Zhong, Heng Wang, Jianji Li, Guoqiang Zhu, Pengpeng Xia, Luying Cui, Jun Li, Junsheng Dong, Qingqing Gao, Xia Meng
Summary: The study confirmed that clbG is essential for the synthesis of genotoxin colibactin in APEC XM strain, and its deletion reduced the likelihood of APEC meningitis in mice.
Article
Veterinary Sciences
Sherif I. A. Mahmoud, Kamel A. Zyan, Mohamed M. Hamoud, Eman Khalifa, Shahin Dardir, Rabab Khalifa, Walid H. Kilany, Wael K. Elfeil
Summary: In this study, the use of LPAIV H9N2 vaccine in commercial broilers showed significant reduction in virus shedding and clinical manifestations, as well as lower mortality rates when co-infected with E. coli. Thus, the LPAIV H9N2 vaccine plays an important role in prevention and control in commercial broilers.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Veterinary Sciences
Xiangchun Ruan, Xiaoling Deng, Meiling Tan, Chengbo Yu, Meishi Zhang, Ying Sun, Nuohao Jiang
Summary: Resveratrol exhibited good antibiofilm activity against APEC in vitro, including inhibiting swimming and swarming motility, biofilm formation, and biofilm eradication.
BMC VETERINARY RESEARCH
(2021)
Article
Microbiology
Andre Becker S. Saidenberg, Arnoud H. M. van Vliet, Marc Stegger, Thor Bech Johannesen, Torsten Semmler, Marcos Cunha, Alessandro C. de O. Silveira, Eleine Kuroki Anzai, Isabel C. A. Scaletsky, Anders Dalsgaard, Roberto M. La Ragione, Terezinha Knobl
Summary: Extraintestinal pathogenic Escherichia coli (ExPEC) is a globally distributed pathogen associated with human and companion animal diseases, while avian pathogenic pathotype (APEC) severely impacts poultry health and production. The similarities between APEC and human ExPEC suggest a potential for zoonotic transmission.
VETERINARY MICROBIOLOGY
(2022)
Article
Microbiology
Koeun Kim, Young Ju Lee
Summary: This study analyzed the relationship between CRISPR sequence type and antimicrobial resistance (AMR) in Avian pathogenic Escherichia coli (APEC) isolates. The results showed that CRISPR sequence type is associated with multidrug resistance and can enhance strategies for tackling antimicrobial resistance in bacterial pathogens.
VETERINARY MICROBIOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Dandan Fu, Jianmei Wu, Yi Gu, Qianwen Li, Ying Shao, Hanshuang Feng, Xiangjun Song, Jian Tu, Kezong Qi
Summary: This study constructed ompR gene mutant and complement strains using the CRISPR-Cas9 system. It was found that inactivation of ompR gene attenuated the pathogenicity of APEC and affected bacterial motility, biofilm formation, and curli production.
Article
Multidisciplinary Sciences
Shouming Zhang, Xueping Gao, Genhui Xiao, Chengping Lu, Huochun Yao, Hongjie Fan, Zongfu Wu
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2018)
Article
Infectious Diseases
Jinhu Huang, Xi Liu, Hao Chen, Li Chen, Xueping Gao, Zihao Pan, Jian Wang, Chengping Lu, Huochun Yao, Liping Wang, Zongfu Wu
TRANSBOUNDARY AND EMERGING DISEASES
(2019)
Article
Microbiology
Zihao Pan, Jin Liu, Yue Zhang, Shenshen Chen, Jiale Ma, Wenyang Dong, Zongfu Wu, Huochun Yao
VETERINARY MICROBIOLOGY
(2019)
Article
Biochemistry & Molecular Biology
David Lalaouna, Jessica Baude, Zongfu Wu, Arnaud Tomasinil, Johana Chicher, Stefano Marzil, Francois Vandenesch, Pascale Romby, Isabelle Caldelari, Karen Moreau
NUCLEIC ACIDS RESEARCH
(2019)
Article
Microbiology
Jiale Ma, Ze Zhang, Zihao Pan, Qiankun Bai, Xiaojun Zhong, Yinchu Zhu, Yue Zhang, Zongfu Wu, Guangjin Liu, Huochun Yao
Article
Immunology
Guangjin Liu, Tingting Gao, Xiaojun Zhong, Jiale Ma, Yumin Zhang, Shiyu Zhang, Zongfu Wu, Zihao Pan, Yinchu Zhu, Huochun Yao, Yongjie Liu, Chengping Lu
INFECTION AND IMMUNITY
(2020)
Article
Microbiology
Yinchu Zhu, Wenyang Dong, Jiale Ma, Yue Zhang, Xiaojun Zhong, Zihao Pan, Guangjin Liu, Zongfu Wu, Huochun Yao
Summary: The study revealed the relatedness between different subpopulations of S. suis serotypes (SS2, SS3, SS7) and capsule switching in CC28 and CC29. Clade 2 SS2 strains showed high genetic similarity with SS3 and SS7, sharing common competence and defensive elements.
MICROBIOLOGICAL RESEARCH
(2021)
Article
Microbiology
Xiaoming Wang, Junjie Sun, Chen Bian, Jianping Wang, Zijing Liang, Yanling Shen, Huochun Yao, Jinhu Huang, Liping Wang, Han Zheng, Zongfu Wu
Summary: The zoonotic pathogen Streptococcus suis SS31 exhibits high heterogeneity, with 75 strains clustered into 3 lineages showing different evolutionary paths and antimicrobial resistance profiles. Additionally, some strains isolated from clinically healthy pigs also showed pathogenicity, highlighting the unique characteristics of SS31 and contributing to public health surveillance and understanding the diversity of S. suis.
VETERINARY MICROBIOLOGY
(2021)
Article
Infectious Diseases
Shuoyue Wang, Miaohang Ma, Zijing Liang, Xinchi Zhu, Huochun Yao, Liping Wang, Zongfu Wu
Summary: This study provides valuable information about the pathogenetic characteristics and antimicrobial resistance of Streptococcus pasteurianus. The capsule was identified as an essential virulence factor for this pathogen, and a mouse infection model was established.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Infectious Diseases
Zhaoying Liu, Qiuhua Xu, Pujun Liang, Zeren Peng, Huochun Yao, Han Zheng, Zongfu Wu
Summary: Streptococcus suis, a severe zoonotic and foodborne pathogen, has distinct population structure and pathogenicity based on various serotypes. This study focused on serotype 8 (SS8) and found that SS8 population exhibited geographical clustering and included strains with global outbreak potential. Several SS8 strains showed virulence in mice and zebrafish, but no specific virulence indicators were identified. All SS8 isolates were classified as multidrug-resistant, with a high prevalence of resistance genes for macrolides, lincosamides, and tetracyclines. These findings highlight the importance of SS8 surveillance.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Immunology
Zijing Liang, Huizhen Wu, Chen Bian, Hao Chen, Yanling Shen, Xueping Gao, Jiale Ma, Huochun Yao, Liping Wang, Zongfu Wu
Summary: This study revealed the mechanism of stable persistence of Streptococcus suis in pig tonsil niche and its competitive survival strategy. It was found that S. suis strain WUSS351 is virulent and multidrug-resistant, and possesses several antimicrobial systems. Additionally, the study demonstrated the T7SS-mediated antagonism of S. suis against other strains. The research also revealed that Lcn351 mainly targets S. suis in pig tonsils. These findings have important implications for the epidemiology and disease progression of S. suis.
Article
Microbiology
Miaohang Ma, Shuoyue Wang, Xinchi Zhu, Xinchun Li, Yinli Bao, Xiang Chen, Zongfu Wu
Summary: Streptococcus pasteurianus is a zoonotic pathogen causing meningitis and bacteremia. This study established a multiplex PCR assay for the detection of S. pasteurianus and found two non-virulent strains with multidrug resistance characteristics. These findings provide important insights into the epidemiology and pathogenesis of this zoonotic bacterium.
Article
Immunology
Xinchi Zhu, Shuoyue Wang, Yu Du, Zijing Liang, Huochun Yao, Xiang Chen, Zongfu Wu
Summary: This study utilized RNA-Seq to reveal the transcriptional landscape of Streptococcus suis under oxidative stress. Several novel genes responsible for resisting oxidative stress were identified, along with a novel aquaporin, Aagp, which plays a crucial role during hydrogen peroxide stress. Additionally, Aagp may be involved in glycerol transport and contributes to S. suis virulence.
Article
Immunology
Pujun Liang, Mingliu Wang, Marcelo Gottschalk, Ana Vela, April A. Estrada, Jianping Wang, Pengcheng Du, Ming Luo, Han Zheng, Zongfu Wu
Summary: This study confirmed the non-negligible pathogenicity of Streptococcus suis serotype 7 and deepened the understanding of its population structure, providing valuable information for improved surveillance of this serotype.
EMERGING MICROBES & INFECTIONS
(2021)
Article
Immunology
Qiankun Bai, Jiale Ma, Ze Zhang, Xiaojun Zhong, Zihao Pan, Yinchu Zhu, Yue Zhang, Zongfu Wu, Guangjin Liu, Huochun Yao