Article
Agriculture, Dairy & Animal Science
Yuhei Kogasaka, Sho Murakami, Shiro Yamashita, Daisuke Kimura, Yoshinori Furumoto, Kana Iguchi, Yutaka Sendai
Summary: In this study, the germline effects of NANOS3 knockout in pigs were investigated using CRISPR/Cas9 technology. The results demonstrated that NANOS3 is crucial for porcine germ cell production.
JOURNAL OF REPRODUCTION AND DEVELOPMENT
(2022)
Article
Reproductive Biology
Kento Miura, Shogo Matoba, Michiko Hirose, Atsuo Ogura
Summary: A CRISPR/Cas9 system utilizing three guide RNAs targeting Nanos3 was able to generate chimeric mice with spermatozoa fully derived from ESCs, confirming that Dnmt3b is not essential for male germ cell development.
BIOLOGY OF REPRODUCTION
(2021)
Review
Agriculture, Dairy & Animal Science
D. P. Berry
Summary: As a larger proportion of beef output comes from dairy herds, dairy producers are making key decisions on genetic merit of carcasses. There is a growing interest in generating valuable calves from dairy females, with the use of selected beef bulls for mating. Developing breeding objectives for beef bulls suitable for dairy production systems can improve profitability and sustainability by exploiting beef-on-dairy breeding strategies.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Endocrinology & Metabolism
Ying-Yi Luo, Hui-Ying Jie, Ke-Jun Huang, Bing Cai, Xiu Zhou, Ming-Yi Liang, Can-Quan Zhou, Qing-Yun Mai
Summary: The expression of SOX17 was found to be dynamic in human female germ cells. A population of SOX17(+) VASA(-) germ cells was discovered in the cortex of fetal ovaries. No relationship between mitosis or meiosis and SOX17 or VASA staining in germ cells was found. These findings provide insight into the potential role of SOX17 in germ cell maturation after specification, although the mechanism is unclear and needs further investigation.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Multidisciplinary Sciences
Morgane Le Rolle, Filippo Massa, Pam Siggers, Laurent Turchi, Agnes Loubat, Bon-Kyoung Koo, Hans Clevers, Andy Greenfield, Andreas Schedl, Marie-Christine Chaboissier, Anne-Amandine Chassot
Summary: This study shows that germ cell-intrinsic beta-catenin activity in mouse embryonic ovaries is essential for maintaining pluripotency and its repression is crucial for differentiation and meiosis entry. The interaction of beta-catenin with the pluripotent-associated factor POU5F1 in the nucleus is associated with germ cell pluripotency, and its exit from the nucleus correlates with germ cell differentiation. These findings highlight the role of beta-catenin as a central gatekeeper in ovarian differentiation and gametogenesis.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biology
Yasunari Seita, Keren Cheng, John R. McCarrey, Nomesh Yadu, Ian H. Cheeseman, Alec Bagwell, Corinna N. Ross, Isamar Santana Toro, Li-hua Yen, Sean Vargas, Christopher S. Navara, Brian P. Hermann, Kotaro Sasaki
Summary: This study successfully established a method for culturing mid-gestation mouse iPSCs on a feeder layer, which can further differentiate into cells similar to primordial germ cells (PGCLCs). Furthermore, these cells can acquire an early developmental germ cell phenotype when co-cultured with mouse testicular somatic cells.
Article
Biology
Hua Yang, Mingtian Deng, Wenli Lv, Zongyou Wei, Yu Cai, Peiyong Cheng, Feng Wang, Yanli Zhang
Summary: Male germ cell development and gene expression play essential roles in male reproduction. In this study, we investigated the expression pattern of four crucial genes (bmp4, dazl, nanos3, and sycp2) in Hu sheep testicular development and constructed an overexpression model using the CRISPR/dCas9 system. Our results showed that the overexpression of these four genes can promote the expression of male germ cell related genes. We also found that these genes are expressed in sheep Leydig cells. This study provides a basis for further male germ cell differentiation in vitro.
Article
Agriculture, Dairy & Animal Science
Lacey M. Dysart, Riley D. Messman, Abigail A. Crouse, Caleb O. Lemley, Jamie E. Larson
Summary: The objectives of the experiments were to determine the effects of exogenous estradiol benzoate on follicular, luteal, and uterine hemodynamics. In Experiment 1, EB treatment resulted in higher estradiol concentrations at Day 0 and higher progesterone concentrations at Day 21. In Experiment 2, EB only affected the corpus luteum on Day 21, with pregnant cows showing larger CL size and blood perfusion.
ANIMAL REPRODUCTION SCIENCE
(2021)
Article
Reproductive Biology
Diksha Sharma, Deepa Bhartiya
Summary: The study reveals the presence of VSELs and OSCs, along with increased numbers of GCNs blocked in pre-meiotic or early meiotic stage in aged ovaries. Ovarian senescence/menopause does not result from accelerated loss of primordial follicles, but rather from dysfunction of stem cells.
JOURNAL OF OVARIAN RESEARCH
(2022)
Article
Agriculture, Dairy & Animal Science
Dongmei Jiang, Qian Sun, Yilong Jiang, Xuemin Zhou, Lijuan Kang, Zelong Wang, Xin Wang, Xiaoguang An, Chengweng Ji, Weikang Ling, Yinglian Wang, Yujie Yang, Bo Kang
Summary: This study found that exogenous spermidine can induce autophagy and inhibit oxidative stress damage in goose ovaries and follicular granulosa cells. The addition of spermidine increased the contents of putrescine, spermidine, and spermine, while reducing ROS levels and apoptosis rate.
JOURNAL OF ANIMAL SCIENCE
(2023)
Review
Andrology
Ying-Hong Cui, Wei Chen, Si Wu, Cai-Lin Wan, Zuping He
Summary: Infertility affects 10%-15% of couples worldwide, with male infertility accounting for 50% of the cases. In China, there has been a significant decrease in the newborn population by 7.82 million in 2020 compared to 2016. Stem cells, including ESCs, iPSCs, SSCs, and MSCs, have the potential to differentiate into male germ cells, and recent progress has been made in generating male germ cells in vitro from stem cells under specific conditions. This review addresses the current advancements in deriving male germ cells from stem cells and discusses their potential applications in reproductive medicine.
ASIAN JOURNAL OF ANDROLOGY
(2023)
Article
Zoology
Toshiya Nishimura, Minoru Tanaka
Summary: Nanos3 plays a crucial role in the maintenance, development, and sex determination of germ cells in medaka. Loss of Nanos3 results in a significant decrease in germ cell number and infertility. Additionally, Nanos3 affects the proper regulation of germline sex in medaka.
ZOOLOGICAL SCIENCE
(2022)
Article
Cell & Tissue Engineering
Joel Zvick, Monika Tarnowska-Sengul, Adhideb Ghosh, Nicola Bundschuh, Pjeter Gjonlleshaj, Laura C. Hinte, Christine L. Trautmann, Falko Noe, Xhem Qabrati, Seraina A. Domenig, Inseon Kim, Thomas Hennek, Ferdinand von Meyenn, Ori Bar-Nur
Summary: This study successfully achieved complementation of functional spermatozoa in adult chimeras through the technique of blastocyst complementation. Using mouse and rat pluripotent stem cells, the researchers were able to generate chimeras with either species-specific sperm or interspecies sperm capable of fertilization. Single-cell RNA sequencing was used to study spermatogenesis in the chimeric testis. The findings of this study have important implications for germ cell research and conservation efforts for endangered animal species.
Article
Reproductive Biology
Paulina Nguyen-Powanda, Bernard Robaire
Summary: The efficiency of antioxidant defense system decreases with aging, resulting in high levels of reactive oxygen species and DNA damage in spermatozoa. This study found that aging and removal of SOD1 (superoxide dismutase-1) lead to increased lipid peroxidation and DNA damage in germ cells.
BIOLOGY OF REPRODUCTION
(2021)
Article
Cell Biology
Chenglei Tian, Linlin Liu, Ming Zeng, Xiaoyan Sheng, Dai Heng, Lingling Wang, Xiaoying Ye, David L. Keefe, Lin Liu
Summary: Research shows that oocytes derived from parthenogenetic embryonic stem cells can produce healthy offspring while maintaining maternal imprinting, offering a promising route for deriving functional oocytes and potentially rebuilding ovarian endocrine function.
