Article
Infectious Diseases
Jiayin Li, Yueying Sheng, Ruijing Ma, Mengsha Xu, Fuli Liu, Rong Qin, Mingxi Zhu, Xianchao Zhu, Ping He
Summary: This study identified and characterized a novel capsule depolymerase K64-ORF41 with specific activity against K. pneumoniae K64-serotype, proving to be an accurate and reliable tool for capsular typing and potentially a therapeutic biologic against CRKP-K64 infections. Mutant strains with altered capsular serotype structure were also discovered, highlighting the importance of the depolymerase in sensitizing bacteria to immune responses.
Article
Microbiology
Yunfen Hua, Yongqin Wu, Minjie Guo, Ruijing Ma, Qingchuan Li, Zheyuan Hu, Hongrui Chen, Xingyu Zhang, Hui Li, Qingtian Li, Ping He
Summary: In this study, a new capsule depolymerase K19-Dpo41 from K. pneumoniae bacteriophage SH-KP156570 was identified and characterized. The depolymerase showed specific activities against K19-type K. pneumoniae and increased its susceptibility to human serum bactericidal effect. Moreover, K19-Dpo41 treatment significantly improved the survival rate of Galleria mellonella infected with K19-type K. pneumoniae. These findings provide preliminary evidence for the potential application of K19-Dpo41 in capsular typing and alternative therapeutic strategies against K19-type CRKP infections.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Roman B. Gorodnichev, Maria A. Kornienko, Maja V. Malakhova, Dmitry A. Bespiatykh, Valentin A. Manuvera, Oksana V. Selezneva, Vladimir A. Veselovsky, Dmitry V. Bagrov, Marina V. Zaychikova, Veronika A. Osnach, Anna V. Shabalina, Oleg V. Goloshchapov, Julia A. Bespyatykh, Anna S. Dolgova, Egor A. Shitikov
Summary: To address the crisis of Klebsiella pneumoniae infections caused by drug-resistant strains, new approaches to antimicrobial therapy, such as using phages or phage derivatives, need to be developed. This study describes the first K. pneumoniae phage from the Zobellviridae family, which was isolated from river water and demonstrates lytic activity against K. pneumoniae strains with the KL20 capsule type. The phage's receptor-binding protein was identified as a polysaccharide depolymerase, showing promise for using depolymerases in antimicrobial therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Anastasiya A. Kasimova, Mikhail M. Shneider, Peter Evseev, Andrey A. Shelenkov, Yuliya Mikhailova, Konstantin A. Miroshnikov, Igor Chebotar, Dmitry A. Shagin
Summary: The study identified the presence of the KL108 gene cluster in clinical isolate of Klebsiella pneumoniae 1333/P225, which is involved in capsule biosynthesis. The structure of the K108 capsular polysaccharide (CPS) was determined using various analysis methods. Additionally, two bacteriophages infecting the 1333/P225 strain were isolated and the genes for structural depolymerases were determined.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Microbiology
Yuchen Wu, Xuemei Yang, Congcong Liu, Yanyan Zhang, Yan Chu Cheung, Edward Wai Chi Chan, Sheng Chen, Rong Zhang
Summary: A new variant of Klebsiella pneumoniae carbapenemase (KPC-93) was identified in clinical isolates, causing resistance to ceftazidime-avibactam. The study highlights the potential risk of bla(KPC) gene mutations associated with ceftazidime-avibactam exposure over a short period of time.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Tamal Dey, Ardhendu Chakrabortty, Aastha Kapoor, Anuja Warrier, Vijaya Lakshmi Nag, Karthikeyan Sivashanmugam, Manoharan Shankar
Summary: Classical and hypervirulent strains of Klebsiella pneumoniae can cause different types of infections. The strain P34 isolated in India shows hypermucoviscosity but lacks known determinants of hypermucoviscosity, suggesting the presence of novel genetic factors.
MICROBIOLOGY SPECTRUM
(2022)
Article
Veterinary Sciences
Takeshi Tsuka, Hiroichi Ozaki, Daisuke Saito, Toshiyuki Murase, Yoshiharu Okamoto, Kazuo Azuma, Tomohiro Osaki, Norihiko Ito, Yusuke Murahata, Tomohiro Imagawa
Summary: CTX-M-2-producing Klebsiella oxytoca strains with high ESBL production rates may spread clonally within a farm, while the bla(CTX-M-2) gene in Klebsiella pneumoniae may disseminate among farms through different plasmids. Monitoring ESBL genes, including bla(CTX-M-2), among causative agents of bacterial mastitis in cows can help develop relevant treatments and control practices.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Microbiology
Tao Chen, Hongyun Xu, Yunbo Chen, Jinru Ji, Chaoqun Ying, Zhiying Liu, Hao Xu, Kai Zhou, Yonghong Xiao, Ping Shen
Summary: A MDR OXA-232-producing K. pneumoniae ST231 clone, closely related to Indian isolates, has been found in China. It is likely that this clone was imported from South Asia. The clone is highly resistant but has low virulence potential.
MICROBIOLOGY SPECTRUM
(2023)
Article
Infectious Diseases
Ahalieyah Anantharajah, Matthieu Deltombe, Marie de Barsy, Stephanie Evrard, Olivier Denis, Pierre Bogaerts, Marie Hallin, Veronique Yvette Miendje Deyi, Denis Pierard, Peggy Bruynseels, Jerina Boelens, Youri Glupczynski, Te-Din Huang
Summary: This study investigated 22 clinical invasive isolates of Klebsiella pneumoniae and found that hypervirulent strains showed increased pathogenicity. The majority of the isolates expressed the K2 capsular serotype and belonged to various MLST lineages, while the remaining strains expressed K1 and were associated with liver abscesses. Among the patients, 36% had primary extra-hepatic infections, 95% had sepsis, and the mortality rate was 30%. Improved clinical and microbiological diagnostics are needed to better understand and manage community-acquired invasive infections caused by hypervirulent K. pneumoniae.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2022)
Article
Microbiology
Han Lin, Jie Ma, Jingyang Sun, Zhendong Qin, Biao Jiang, Wei Li, Qing Wang, Youlu Su, Li Lin, Chun Liu
Summary: From September to October 2021, a high-mortality disease outbreak occurred in a bullfrog farm in Guangzhou, China. The infected bullfrogs showed multiple organ congestion and inflammation. The pathogenic bacterium isolated from the infected bullfrogs was confirmed to be carbapenemase-producing multidrug-resistant K. pneumoniae (MDR-KP) strain, and this is the first report of K. pneumoniae infected bullfrogs and amphibians.
MICROBIOLOGY SPECTRUM
(2023)
Article
Chemistry, Applied
I-Ming Lee, Tzu-Yin Huang, Feng-Ling Yang, Victor Johansson, Chun-Ru Hsu, Pei-Fang Hsieh, Shin-Tai Chen, Yen-Ju Yang, Peter Konradsson, Jyh-Horng Sheu, Jin-Town Wang, Shih-Hsiung Wu
Summary: This study identified the structure of capsular polysaccharide of Klebsiella pneumoniae KN2 and demonstrated its ability to modulate immune response and stimulate macrophages to release immune-related factors. The study also found that hexasaccharide can serve as a potential vaccine adjuvant.
CARBOHYDRATE POLYMERS
(2022)
Article
Virology
Min Li, Hui Wang, Long Chen, Genglin Guo, Pei Li, Jiale Ma, Rong Chen, Hong Du, Yuqing Liu, Wei Zhang
Summary: In this study, phage P560 isolated from sewage showed specificity for capsule locus type KL47 K. pneumoniae. The capsule depolymerase P560dep derived from phage P560 was able to degrade the capsule polysaccharides of KL47 type K. pneumoniae and inhibit biofilm formation. Additionally, a single dose of P560dep protected mice from lethal challenge and alleviated pathological changes in the lungs and livers of mice infected with KL47 type K. pneumoniae.
Article
Immunology
Jiehui Qiu, Dandan Wei, Jiaxin Ma, Ren Liu, Jianglong Shi, Qun Ren, Chunping Wei, Binghui Huo, Lanlan Zhu, Tianxin Xiang, Yang Liu, Na Cheng
Summary: This study examined the epidemiology, clinical characteristics, and molecular and virulence attributes of Klebsiella pneumoniae serotype K54 (K54-Kp). It was found that K54-Kp had a high resistance rate to commonly used antibiotics and patients with hepatobiliary diseases were more susceptible to K54-Kp infection. The strains primarily carried several virulence genes and exhibited strong biofilm formation ability. However, the virulence of K54-Kp was lower compared to other types in the Galleria mellonella model.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Fuqiang Kang, Zili Chai, Beiping Li, Mingda Hu, Zilong Yang, Xia Wang, Wenting Liu, Hongguang Ren, Yuan Jin, Junjie Yue
Summary: This study identified 15,946 prophages from 1437 fully assembled K. pneumoniae genomes deposited in the NCBI database, with 9755 prophages on chromosomes and 6191 prophages on plasmids. The results showed that prophages in the K. pneumoniae genomes are diverse and widely distributed. The prophages encoded multiple putative virulence factors and antibiotic resistance genes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Kasturi Banerjee, Michael P. Motley, Camila Boniche-Alfaro, Somanon Bhattacharya, Raj Shah, Andrew Ardizzone, Bettina C. Fries
Summary: In this study, we present in vitro and in vivo data demonstrating the cross-protective efficacy of the wzi50 CPS-specific monoclonal antibody MAb 24D11 against three prominent win types (wzi29, wzi154, and wzi50) of the carbapenem-resistant clonal group CG258. In a murine pulmonary infection model, MAb 24D11 reduced bacterial lung burden and dissemination to other organs even if administered 4 h postinfection, and its protective efficacy was also observed in neutropenic mice, highlighting its potential value in clinical settings.
MICROBIOLOGY SPECTRUM
(2022)
Article
Immunology
Ying-Chun Chien, Yu-Tsung Huang, Chun-Hsing Liao, Jung-Yien Chien, Po-Ren Hsueh
Summary: This study investigated the clinical characteristics and outcomes of bacteremia caused by Haemophilus and Aggregatibacter species in patients treated at a medical center between 2006 and 2018. The results showed that the majority of patients with bacteremia had community-acquired disease, with a higher Sequential Organ Failure Assessment (SOFA) score being an independent predictor of poor 7-day clinical outcomes in patients with community-acquired H. influenzae bacteremia.
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION
(2021)
Article
Infectious Diseases
Yu-Tsung Huang, Yao-Wen Kuo, Meng-Rui Lee, Yu-Huan Tsai, Lee-Jene Teng, Mao-Song Tsai, Chun-Hsing Liao, Po-Ren Hsueh
Summary: The study in Taiwan found that non-perinatal listeriosis cases were more common in males with an average age of 63.9 years, most of whom had underlying conditions. The 30-day all-cause mortality rate was 25.2%, which was associated with age, steroid usage, and respiratory distress.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Immunology
Jih-Hsin Huang, Hou-Tai Chang, Chun-Hsing Liao, Kuan-Ming Chiu
Summary: A surge of COVID-19 cases in northern Taiwan posed a crisis to the medical system, but Far Eastern Memorial Hospital (FEMH) responded quickly and increased its capacity through various measures such as reducing hospitalization cases, expanding beds, and conducting extensive testing. With determined leadership and concerted efforts, FEMH successfully combated the epidemic.
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION
(2022)
Article
Engineering, Environmental
Chih-Kuei Chen, Nhat-Thien Nguyen, Thuy-Trang Le, Cong-Chinh Duong, Cong-Nguyen Nguyen, Duc-Toan Truong, Chun-Hsing Liao
Summary: This study utilized modified sludge biochar for arsenic adsorption, significantly increasing its adsorption potential. Using Density Functional Theory (DFT) for the first time, the research simulated the adsorption mechanism of arsenic and optimized operation conditions.
WATER SCIENCE AND TECHNOLOGY
(2022)
Article
Infectious Diseases
Johannes Camp, Tim Filla, Lina Glaubitz, Achim J. Kaasch, Frieder Fuchs, Matt Scarborough, Hong Bin Kim, Robert Tilley, Chun-Hsing Liao, Jonathan Edgeworth, Emmanuel Nsutebu, Luis Eduardo Lopez-Cortes, Laura Morata, Martin J. Llewelyn, Vance G. Fowler, Guy Thwaites, Harald Seifert, Winfried Kern, Siegbert Rieg
Summary: This study investigated the impact of neutropenia on the mortality and long-term outcomes of Staphylococcus aureus bloodstream infection. The results showed that neutropenia was not associated with a higher survival rate during follow-up, but neutropenic patients had a lower rate of SAB infection-related late complications.
CLINICAL MICROBIOLOGY AND INFECTION
(2022)
Article
Urology & Nephrology
Kuei-Ting Tung, Yu-Sen Peng, Shih-Ping Hsu, Hon-Yen Wu, Yen-Ling Chiu, Ju-Yeh Yang, Mei-Fen Pai, Kai-Hsiang Shu, Szu-Yu Pan, Hui-Ming Lu, Wan-Yu Lin, Chun-Hsing Liao, Fang-Yeh Chu, Wan-Chuan Tsai
Summary: This study aimed to explore whether Asian hemodialysis patients can effectively produce an immune response after being vaccinated with the first dose of the ChAdOx1 nCoV-19 vaccine. The results showed that the seropositive rate in hemodialysis patients was low, and most had low antibody titers after the first dose of the vaccine. Younger age, better dialysis adequacy, and higher albumin levels were associated with seropositivity.
HEMODIALYSIS INTERNATIONAL
(2022)
Article
Microbiology
Chun-Hsing Liao, Yu-Tsung Huang, Po-Ren Hsueh
Summary: In this study, a longitudinal epidemiological surveillance of hypervirulent Klebsiella pneumoniae (hvKP) in Taiwan was conducted. K1 remained the dominant serotype of hvKP and was associated with most virulence genes.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Immunology
Fang -Fang Hsu, Chia-Jui Yang, Mao -Song Tsai, Hsih-Yeh Tsai, Hong -An Chen, Chun-Hsing Liao
Summary: Prompt infection control measures and large-scale PCR screening can control a COVID-19 outbreak within 2 weeks. Exposure time is the major risk factor for HCW infection.
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION
(2022)
Letter
Infectious Diseases
Jia-Arng Lee, Shin-Hei Du, Tai-fen Lee, Yu-Shan Huang, Chun-Hsing Liao, Yu-Tsung Huang, Po-Ren Hsueh
JOURNAL OF INFECTION
(2023)
Article
Immunology
Hon-Yen Wu, Yi-Tsung Lin, Wan-Chuan Tsai, Yen-Ling Chiu, Mei-Ju Ko, Ju-Yeh Yang, Mei-Fen Pai, Hsin-Sui Hsu, Shih-Ping Hsu, Yu-Sen Peng, Chun-Hsing Liao
Summary: This study aimed to investigate the distribution of Enterobacteriaceae in the gut microbiota of hemodialysis patients and analyze its association with clinical parameters. The study found significant differences in the gut microbiota composition at the genus level between hemodialysis patients and the control group, with an increase in the Escherichia-Shigella complex and an association between Klebsiella and emergency room visits. Therefore, further research on the microbiota composition of Enterobacteriaceae is necessary.
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION
(2023)
Article
Infectious Diseases
Chun-Hsing Liao, Ren-Hsuan Ku, Li-Hua Li, Cheng-Mu Wu, Tsuey-Ching Yang
Summary: In our recent study, we found that the yceA-cybB-yceB gene cluster is up-regulated in the presence of oxidative stress. This gene cluster plays a role in alleviating oxidative stress, enhancing swimming motility, and increasing susceptibility to certain antibiotics.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Infectious Diseases
Bing-Jie Shen, Jann-Tay Wang, Hou-Tai Chang, Shan-Chwen Chang, Chun-Hsing Liao
Summary: Active surveillance for sputum cultures revealed Burkholderia cepacia infection in an intensive care unit from September 2008 to September 2009. The use of disposable ventilator circuits resulted in a decrease in infection rates, and this effect persisted in the following years.
TROPICAL MEDICINE AND INFECTIOUS DISEASE
(2023)
Article
Medicine, Research & Experimental
Chang Yi Wang, Kao-Pin Hwang, Hui-Kai Kuo, Wen-Jiun Peng, Yea-Huei Shen, Be-Sheng Kuo, Juin-Hua Huang, Hope Liu, Yu-Hsin Ho, Feng Lin, Shuang Ding, Zhi Liu, Huan-Ting Wu, Ching-Tai Huang, Yuarn-Jang Lee, Ming-Che Liu, Yi-Ching Yang, Po-Liang Lu, Hung-Chin Tsai, Chen-Hsiang Lee, Zhi-Yuan Shi, Chun-Eng Liu, Chun-Hsing Liao, Feng-Yee Chang, Hsiang-Cheng Chen, Fu-Der Wang, Kuo-Liang Hou, Jennifer Cheng, Min-Sheng Wang, Ya-Ting Yang, Han-Chen Chiu, Ming-Han Jiang, Hao-Yu Shih, Hsuan-Yu Shen, Po-Yen Chang, Yu-Rou Lan, Chi-Tian Chen, Yi-Ling Lin, Jian-Jong Liang, Chun-Che Liao, Yu-Chi Chou, Mary Kate Morris, Carl V. Hanson, Farshad Guirakhoo, Michael Hellerstein, Hui-Jing Yu, Chwan-Chuen King, Tracy Kemp, D. Gray Heppner, Thomas P. Monath
Summary: The UB-612 vaccine showed favorable safety profile, long-lasting antibody response, and broad T cell immunity against SARS-CoV-2 variants, including Delta and Omicron, in phase I/II trials.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Article
Cell Biology
Chun-Hsing Liao, Hsu-Feng Lu, Hsin-Hui Huang, Yu Chen, Li-Hua Li, Yi-Tsung Lin, Tsuey-Ching Yang
Summary: This study focused on elucidating the ferric citrate acquisition system of S. maltophilia, revealing the involvement of FciTABC and FeoABI systems in ferric citrate acquisition.
JOURNAL OF BIOMEDICAL SCIENCE
(2022)
Article
Microbiology
Yu-Tsung Huang, Yao-Wen Kuo, Nan-Yao Lee, Ni Tien, Chun-Hsing Liao, Lee-Jene Teng, Wen-Chien Ko, Po-Ren Hsueh
Summary: Detection of carbapenemase-producing Enterobacterales is important for therapeutic decisions and infection control. The Xpert CARBA-R and NG-Test CARBA 5 assays showed high sensitivity and specificity in detecting carbapenemases in carbapenem-resistant Enterobacterales isolates.
MICROBIOLOGY SPECTRUM
(2022)