Article
Biochemistry & Molecular Biology
Juan Palacios-Ortega, Esperanza Rivera-de-Torre, Sara Garcia-Linares, Jose G. Gavilanes, Alvaro Martinez-del-Pozo, J. Peter Slotte
Summary: Sticholysins are pore-forming toxins produced by sea anemones, capable of oligomerizing in solution and forming pores with specific stoichiometry. The study also indicates that the stoichiometry of actinoporin pores is conserved in equilibrium, regardless of the particular composition.
Article
Chemistry, Applied
Ashton N. Bartley, Sadie F. Depeter, Ronald K. Castellano
Summary: This article reports the preparation and photophysical investigation of the first molecular multi-FRET system from the benzotrifuranone (BTF) scaffold. The target molecule was synthesized using a step-conservative approach, incorporating three optically complementary chromophores. The study demonstrates high energy transfer efficiency and significant pseudo-Stokes shift in the fluorescence emission of the target. The design has potential applications in material science and life sciences.
Review
Chemistry, Multidisciplinary
Bolong Zhang, Guanpeng Lyu, Elaine A. Kelly, Rachel C. Evans
Summary: This article introduces the potential of Förster resonance energy transfer (FRET) in enhancing the performance of luminescent solar concentrators (LSCs), including key criteria and interactions with the host material. The authors aim to showcase the potential of FRET-LSCs in both conventional solar harvesting and emerging LSC-inspired technologies, while also encouraging diverse participation from researchers by addressing unanswered questions in this field.
Article
Chemistry, Multidisciplinary
Mingce Tian, Yaping Zhu, Weijiang Guan, Chao Lu
Summary: Measuring drug release dynamics at the subcellular level is crucial for improving therapeutic efficacy and reducing side effects. A novel gemini fluorescent surfactant capable of targeting mitochondria and responding to changes in redox conditions is designed to address this challenge. A quantitative FRET platform is developed using this mitochondria-targeted fluorescent nanocarrier and fluorescent drugs, enabling real-time measurement of drug release from organelle-targeted nanocarriers. This platform offers a new quantitative method for evaluating organelle-targeted drug release and provides insights into drug release behaviors at the subcellular level.
Article
Chemistry, Analytical
Roberto F. Delgadillo, Katie A. Carnes, Kathia Zaleta-Rivera, Omar Olmos, Lawrence J. Parkhurst
Summary: The time-resolved donor-detected Forster resonance energy transfer (trDDFRET) allows observation of molecular interactions within 10-100 angstrom region, while the time-resolved acceptor-detected FRET (trADFRET) can observe longer-range interactions. A new methodology based on trADFRET, namely FLIM-trADFRET, has been proposed to observe biological machinery in the range of 100-300 A in vivo. Proof of concept was demonstrated with a set of well-defined DNA scaffolds to evaluate this new methodology.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Yang Zou, Saran Long, Tao Xiong, Xueze Zhao, Wen Sun, Jianjun Du, Jiangli Fan, Xiaojun Peng
Summary: In this study, a new strategy using the single-molecule Forster resonance energy transfer (smFRET) mechanism was developed to transfer part of the fluorescent energy into heat for combined photodynamic and photothermal therapy. This approach not only improves treatment efficiency but also enables fluorescence imaging. The strategy allows for a combination treatment outcome at relatively low concentrations and light doses.
ACS CENTRAL SCIENCE
(2021)
Article
Biochemical Research Methods
Camille Dubois, Ludivine Houel-Renault, Marie Erard, Nada N. Boustany, Nathalie Westbrook
Summary: The study emphasizes the importance of understanding forces within cells for tissue growth. The researchers demonstrate the use of a cost-effective setup to measure FRET efficiency, allowing for easier study of these forces.
JOURNAL OF BIOMEDICAL OPTICS
(2023)
Article
Materials Science, Multidisciplinary
Sameer Al-Bati, Mohammad Hafizuddin Hj Jumali, Khatatbeh Ibtehaj, Bandar Ali Al-Asbahi, Chi Chin Yap
Summary: The study comprehensively investigated the energy transfer mechanism between different polymers and calculated various energy transfer parameters to confirm the occurrence of energy transfer and its influencing factors. Spectral analysis and parameter calculation provided insights into the energy transfer process in the three binary blends studied.
Article
Biochemistry & Molecular Biology
Fiona Gordon, Saioa Elcoroaristizabal, Alan G. Ryder
Summary: The study presents a novel quantitative FRET analysis using ARMES in a HSA and ANS model, resolving fluorophore contributions spectrally through pEEM matrices and multivariate analysis. The results show increased accuracy in calculating FRET efficiency with the new method, utilizing PARAFAC and Tucker3 for multivariate analysis.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2021)
Article
Chemistry, Multidisciplinary
David Guzman, Ilias Papadopoulos, Giulia Lavarda, Parisa R. Rami, Rik R. Tykwinski, M. Salome Rodriguez-Morgade, Dirk M. Guldi, Tomas Torres
Summary: The study demonstrates the efficient energy transfer between a subporphyrazine and a pentacene dimer through intramolecular Forster resonance energy transfer and intramolecular singlet fission. The polarity of the solvent plays a crucial role in adjusting spectral overlap, ensuring a significant Forster rate constant, and maximizing triplet quantum yields.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Biochemistry & Molecular Biology
Nyema M. Harmon, Xueting Huang, Chia-Hung Christine Hsiao, Andrew J. Wiemer, David F. Wiemer
Summary: Cell-cleavable protecting groups combined with fluorescent groups can be an effective strategy for constructing biological probes to address issues such as stability, solubility, and cellular uptake. A novel synthesis method was utilized to successfully create a phosphonate compound containing a FRET pair, which can deliver payloads to intracellular receptors. This study demonstrates the potential of bioactivatable phosphonates with FRET pairs in developing probes for monitoring cellular uptake of otherwise charged payloads.
BIOORGANIC CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Aleksandra Pilch-Wrobel, Agata Maria Kotulska, Satu Lahtinen, Tero Soukka, Artur Bednarkiewicz
Summary: This study evaluates the designed virtual core-shell NP models for UCNPs and proposes new designs of UCNPs as FRET donors. The luminescence kinetics of UCNP donors during RET and new disparity measurements are discussed. These studies help understand the energy transfer and migration between lanthanide ion dopants in UCNPs and how the core-shell architecture affects their performance as FRET donors to organic acceptor dyes.
Article
Chemistry, Multidisciplinary
Shuting Xiao, Yi Yao, Shuilin Liao, Bin Xu, Xue Li, Yuxiao Zhang, Lei Zhang, Qiang Chen, Haoneng Tang, Qibin Song, Ming Dong
Summary: Researchers have developed an EV-MPDS system based on FRET signals, which allows convenient diagnosis of lung cancer without the need for EV extraction and purification. In clinical samples, this system demonstrated improved accuracy and sensitivity compared to the ELISA detection method, with early screening accuracy further enhanced through machine learning analysis of five biomarkers.
Article
Chemistry, Multidisciplinary
Xin Zou, Nan Gan, Mengyang Dong, Wenguang Huo, Anqi Lv, Xiaokang Yao, Chengzhu Yin, Ziyang Wang, Yuanyuan Zhang, Huan Chen, Huili Ma, Long Gu, Zhongfu An, Wei Huang
Summary: A strategy for achieving narrowband organic afterglow materials through Förster resonance energy transfer from long-lived phosphorescence donors to narrowband fluorescence acceptors in a polyvinyl alcohol matrix is presented. The resulting materials exhibit narrowband emission with a full width at half maximum (FWHM) as small as 23 nm and the longest lifetime of 721.22 ms. By pairing the appropriate donors and acceptors, multicolor and high color purity afterglow ranging from green to red with the maximum photoluminescence quantum yield of 67.1% are achieved. Furthermore, the potential applications in high-resolution afterglow displays and dynamic and quick information identification in low-light conditions are demonstrated.
ADVANCED MATERIALS
(2023)
Article
Biochemical Research Methods
Mehavesh K. Hameed, Javad B. M. Parambath, Sofian M. Kanan, Ahmed A. Mohamed
Summary: Biocompatible and luminescent nanostructures synthesized by capping gold-carbon nanoparticles with amino acids were successfully utilized for the quantitative estimation of the drug ranitidine. The method showed a detection limit of 0.174, 0.56, and 0.332 mu M for different amino acid bioconjugates, and was also successful in quantifying ranitidine in spiked serum samples. The study demonstrates the potential of this approach for sensitive and accurate drug quantification.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Lauren E. Rylaarsdam, Grace N. Johnecheck, Brendan D. Looyenga, Larry L. Louters
Article
Environmental Sciences
Evans K. Lodge, Lawrence S. Engel, Sara Ferrando-Martinez, Derek Wildman, Monica Uddin, Sandro Galea, Allison E. Aiello
JOURNAL OF EXPOSURE SCIENCE AND ENVIRONMENTAL EPIDEMIOLOGY
(2020)
Letter
Education, Scientific Disciplines
Evans K. Lodge
JOURNAL OF SURGICAL EDUCATION
(2020)
Article
Biochemistry & Molecular Biology
Evans K. Lodge, Jedediah D. Bell, Emily M. Roloff, Kathryn E. Hamilton, Larry L. Louters, Brendan D. Looyenga
Article
Biochemistry & Molecular Biology
Miranda F. Bouwer, Kathryn E. Hamilton, Patrick B. Jonker, Sam R. Kuiper, Larry L. Louters, Brendan D. Looyenga
Summary: The study found that NMS-873 rapidly induces aerobic fermentation in human and mouse cells and unexpectedly inhibits mitochondrial oxidative phosphorylation as a dual inhibitor of Complex I and ATP synthase. This suggests that caution is needed when interpreting cell survival data associated with NMS-873 treatment, as cellular toxicity may be caused by both VCP/p97-dependent and VCP/p97-independent mechanisms.
Article
Infectious Diseases
Evans K. Lodge, Annakate M. Schatz, John M. Drake
Summary: This study found significant changes in population isolation rates across multiple outbreaks of EVD, SARS, and MERS, which is crucial for disease modelers to predict the spread of emerging pathogens.
BMC INFECTIOUS DISEASES
(2021)
Article
Public, Environmental & Occupational Health
Evans K. Lodge, Cathrine Hoyo, Carmen M. Gutierrez, Kristen M. Rappazzo, Michael E. Emch, Chantel L. Martin
Summary: The study reveals significant disparities in crime exposure based on participant race and ethnicity, influenced by crime type and measurement method. Therefore, thoughtful consideration is needed when using police-reported crime data to account for joint exposure to crime and police.
Article
Public, Environmental & Occupational Health
Alexis C. Dennis, Esther O. Chung, Evans K. Lodge, Rae Anne Martinez, Rachel E. Wilbur
Summary: The article highlights the significant impact of structural racism on health inequalities in the United States, presenting a new framework to illustrate the unequal effects of structural racism on health opportunities among different racial and ethnic groups.
ETHNICITY & DISEASE
(2021)
Article
Biochemistry & Molecular Biology
Kathryn E. Hamilton, Miranda F. Bouwer, Larry L. Louters, Brendan D. Looyenga
Summary: The study suggests that fluorescent analogs 2-NBDG and 6-NBDG may enter mammalian cells through mechanisms independent of transporters, casting doubt on their utility as accurate proxies for glucose transport.
Article
Environmental Sciences
Evans K. Lodge, Chantel L. Martin, Rebecca C. Fry, Alexandra J. White, Cavin K. Ward-Caviness, Sandro Galea, Allison E. Aiello
Summary: Vacant, aged, and poorly maintained housing may contribute to low-level lead exposure among adults in older cities like Detroit. US Census and neighborhood quality data can be used to identify population-level lead exposure among US adults.
JOURNAL OF EXPOSURE SCIENCE AND ENVIRONMENTAL EPIDEMIOLOGY
(2023)
Article
Public, Environmental & Occupational Health
Evans K. Lodge, Zakiya Haji-Noor, Carmen M. Gutierrez, Allison E. Aiello, Cathrine Hoyo, Michael E. Emch, Chantel L. Martin
Summary: Gestational exposure to violent crime and racialized drug policing is associated with increased blood pressure in early childhood, specifically among children born to Black participants.
Article
Genetics & Heredity
Evans K. Lodge, Radhika Dhingra, Chantel L. Martin, Rebecca C. Fry, Alexandra J. White, Cavin K. Ward-Caviness, Agaz H. Wani, Monica Uddin, Derek E. Wildman, Sandro Galea, Allison E. Aiello
Summary: The effects of lead, mercury, manganese, and copper on individual disease processes have been well understood. However, estimating the health effects of long-term exposure to these metals at low concentrations is difficult. This study aimed to estimate the effects of these metals on biomarkers of biological aging. The results showed positive associations between lead and GrimAge acceleration, and mercury and PhenoAge acceleration, while negative associations were found between manganese and PhenoAge acceleration, and mercury and Horvath Age acceleration. Additionally, curvilinear relationships were observed between copper and both PhenoAge and GrimAge acceleration. Increasing total exposure to the mixture of metals was associated with accelerated biological aging. Despite few statistically significant findings, the consistency and novelty of the results warrant further interest.
ENVIRONMENTAL EPIGENETICS
(2022)
Article
Psychology, Clinical
Janine N. Baldino, Evans K. Lodge, Rita M. Lahlou
Summary: The study found that TGNC individuals had more convenient access to healthcare through telehealth; the main advantages include reduced transportation burden, more efficient use of time, and more frequent communication with student volunteer providers.
TRANSGENDER HEALTH
(2022)
Article
Physiology
Kylee A. Rosette, Stephen M. Lander, Calvin VanOpstall, Brendan D. Looyenga
Summary: The study showed that coculturing tumor cells and fibroblasts in a structured manner more accurately mimics the behavior of papillary renal cell carcinoma, highlighting the importance of stromal fibroblasts in this disease model. Monoculture of tumor cells in two-dimensional settings fails to replicate important features of the disease.
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2021)
Article
Health Care Sciences & Services
Emily J. Ciccone, Paul N. Zivich, Evans K. Lodge, Deanna Zhu, Elle Law, Elyse Miller, Jasmine L. Taylor, Suemin Chung, Jason Xu, Alexander Volfovsky, Cherese Beatty, Haley Abernathy, Elise King, Haley E. Garrett, Alena J. Markmann, Meghan E. Rebuli, Subhashini Sellers, David J. Weber, Raquel Reyes, Naseem Alavian, Jonathan J. Juliano, Ross M. Boyce, Allison E. Aiello
Summary: This study aims to determine the incidence and risk factors of SARS-CoV-2 infection among healthcare personnel at a tertiary care medical center, while actively monitoring PPE use, interactions between participants, secondary cases in households, and participant mental health. Data collection is ongoing and expected to continue through September 2021, with the goal of understanding SARS-CoV-2 transmission risks in healthcare settings and the community.
JMIR RESEARCH PROTOCOLS
(2021)