Article
Fisheries
Weilong Wang, Qingping Lian, Yuange Chen, Naoshi Hiramatsu, Meiqin Wu
Summary: The study developed a method for detecting estrogenic activities in aquatic environments by developing polyclonal antibodies against subtype-specific Vtgs of the dojo loach. It was found that VtgAo1 protein was the major Vtg type in this species and successfully used for Western blotting analysis, indicating induction of VtgAo1 protein after exposure to EE2 and E2.
Article
Biology
Li-Fei Luo, Zi-Sheng Xu, Eman Abdelwareth Baioumy Elsayed Elgazzar, Hang Du, Dan-Yang Li, Xiao-Yun Zhou, Ze-Xia Gao
Summary: Misgurnus anguillicaudatus exhibits both sexual size dimorphism and polyploid size dimorphism. This study found that differences in energy metabolism and steroid hormone synthesis levels may play a key role in the growth dimorphisms observed. Fast-growing individuals (tetraploids, females) showed higher energy metabolism levels and lower abilities in steroid hormone synthesis and fatty acid degradation compared to slow-growing individuals (diploids, males). The differential gene expression analysis between different ploidy levels and sexes revealed potential regulatory genes involved in glycolysis, oxidative phosphorylation, steroid hormone biosynthesis, and fatty acid metabolism pathways. This suggests that metabolic differences may contribute to the growth dimorphisms observed in Misgurnus anguillicaudatus.
Article
Fisheries
Shuaitong Li, Jiahong Zou, Xu Wang, Zixi Song, Zhen Xu, Qingchao Wang
Summary: In this study, glycyrrhizic acid (GA) was applied in the egg hatching and larviculture of dojo loach, showing that low dosage of GA could improve hatchability while high dosage inhibited larval body length growth.
Article
Environmental Sciences
Na Yang, Chaochao Yang, Ting Tan, Qi Wang, Xin Lei
Summary: This study investigated the mechanism of loach testis response to phenanthrene (PHE) and obtained a large amount of gene expression information related to metabolism, spermatogenesis, and immunity genes from RNA-seq.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Immunology
Yayuan Xu, Xinxin Zhang, Dongqi Li, Kun Qian, Yu Liu, Tingjuan Xu, Lishang Dai, Jianghua Cheng
Summary: This study investigated the impact of fermented soybean meal on the immune system of loach by giving LPS-injected loach the fermented soybean powder and using transcriptome sequencing. The results showed significant differential expression in genes involved in cellular processes, metabolic processes, cellular anatomical entities, and binding. The KEGG enrichment analysis revealed that the soybean fermented powder treated groups had significant differences in DNA replication, Nucleotide excision repair, Fanconi anemia pathway, and Base excision repair pathways.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Zeyu Jin, Manli Shen, Liuen Wang, Cui Wang, Miaomiao Gao, Guoying Yu, Zhongjie Chang, Xiaowen Zhang
Summary: A new antimicrobial peptide gene (Ma-Hep) was obtained and found to have antibacterial activity in loaches, providing protection against bacterial infection. Furthermore, feed supplemented with Ma-Hep expressing yeast improved the intestinal flora of loaches and reduced mortality during bacterial infection.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Fisheries
Xiaoli Ke, Jie Liu, Fengying Gao, Jianmeng Cao, Zhigang Liu, Maixin Lu
Summary: Due to environmental pollution and overfishing, the genetic resources of wild loach have been degraded. The study investigated the genetic diversity of different geographical populations of loach in the Pearl River Basin and found that there is abundant genetic diversity, which can be used for breeding. The results provide a theoretical basis for the protection and utilization of loach natural resources in the Pearl River Basin of China.
AQUACULTURE REPORTS
(2022)
Review
Fisheries
Katsutoshi Arai
Summary: The review article "A Review of Dojo Loach Research" summarizes the results of previous studies using the dojo loach as a model animal. It investigates the interrelationship between the constitution of chromosome sets, meiotic configuration, and gametogenesis to better understand the reproductive mechanisms of hybrids, polyploids, and clonal organisms. The study found that autopolyploids with an even number of extra sets of chromosomes were fertile, while autopolyploids with an odd number of extra sets and allopolyploids exhibited a range of sterility. These findings contribute to the understanding of reproductive biology and genetics in fishes.
Article
Biodiversity Conservation
Masamichi Kuroda, Takafumi Fujimoto, Etsuro Yamaha, Katsutoshi Arai
Summary: In most Japanese populations of dojo loach, genetically diversified diploid groups A and B coexist with unisexual clonal lineages. By improving the PCR-RFLP analysis of the RAG1 gene, different fragment patterns were observed for each group, allowing for quick and accurate group identification. This genetic study also detected two different hybrid genotypes in the loach populations.
CONSERVATION GENETICS RESOURCES
(2021)
Article
Fisheries
Guang Yang, Xiaolin Cui, Shu Liu, Jing Lu, Xiaoyue Hou, Wenrong Meng, Bofei Wu, Yongcheng Su, Hongyu Zhang, Wei Zheng, Yaowei Fang
Summary: This study investigated the effects of dietary supplementation of Lactobacillus helveticus HML037 on the growth performance, innate immunity, disease resistance, and intestinal health of pond loaches. The results showed significant improvements in growth performance, immune response, and gut microbiota, particularly at the level of 1 x 10(8) CFU g(-1) of L. helveticus HML037.
Article
Fisheries
Hui-Min Lin, Chen Zeng, Shan-Shan Shui, Bin Zhang
Summary: The study investigated the effects of Fe(II)-chelating hairtail protein hydrolysates on digestive enzymes, nonspecific immunity, and intestinal microbial community in loach. Fe(II)-HPH was found to impact enzyme activities and microbial composition in the gut. Different concentrations of Fe(II)-HPH showed varying effects on enzyme activities and microbial communities in the loach intestine.
AQUACULTURE REPORTS
(2021)
Article
Biotechnology & Applied Microbiology
Bintong Yang, Haichao Song, Renge Hu, Luotao Tao, Zhenlin Liang, Wei Cong, Yuanhuan Kang
Summary: This study screened two representative strains of lactic acid bacteria, Weissella confusa N17 and Lactobacillus saniviri N19, from the intestine of healthy loaches, which showed excellent traits including inhibiting pathogenic bacteria, biofilm formation ability, and adhesion to gut cells. Among them, W. confusa N17 exhibited better protection against A.veronii infection. Therefore, W. confusa N17 holds great potential for further applications in loach aquaculture.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2023)
Article
Fisheries
Ziyu Zhou, Gu Shan, Xin Li, Wenxuan Guo, Kexin Ma, Yueyao Yang, Yifan Li, Yunbang Zhang, He Zhou, Xiaojuan Cao
Summary: This study successfully created hybrid triploid loaches with characteristics of fast growth, high-quality meat, high edibility, high disease resistance, and sterility by crossing a natural tetraploid loach and a diploid loach. Through histological observations and transcriptome analyses, 10 differentially expressed genes related to the early fertility mechanism and two signal pathways closely related to the early fertility mechanism were identified. These findings laid a foundation for further research on the molecular inhibition mechanism in hybrid triploid loaches.
Article
Biochemistry & Molecular Biology
Junxiao Su, Shaokui Yi, Zexia Gao, Khalid Abbas, Xiaoyun Zhou
Summary: DNA methylation plays a crucial role in regulating the expression patterns of aromatase genes cyp19a1a and cyp19a1b during gametogenesis in Misgurnus anguillicaudatus. Methylation negatively regulates the expression of cyp19a1a in the ovaries, while cyp19a1b expression fluctuates in the brain but maintains high methylation levels. This study highlights the importance of DNA methylation in mediating gonadal development in teleosts.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Physiology
Mengya Wang, Wenyu Xu, Jiahong Zou, Shuaitong Li, Zixi Song, Feifei Zheng, Wei Ji, Zhen Xu, Qingchao Wang
Summary: The study found that 400mg/L H2O2 treatment significantly improved loach hatchability and reduced egg mortality, while 200mg/L treatment showed no significant effects. NaCl treatment had no significant impact on loach hatchability or egg mortality. Results indicated a potential role of 400mg/L H2O2 in enhancing antioxidant capacity and immune response in fish larvae through promoting early development.
FRONTIERS IN PHYSIOLOGY
(2021)