Journal
SCIENTIFIC REPORTS
Volume 6, Issue -, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/srep22354
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Funding
- Spanish Ministerio de Economia y Competitividad
- European Seventh Framework Programme (FP7) under the project MULTIFUN grant from the Spanish Ministerio de Economia y Competitividad [262943, BFU2014-54181, BIO2013-44647-R]
- Madrid regional government [S2013/MIT-2850, S2010/BMD-2305]
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We used soft X-ray three-dimensional imaging to quantify the mass of superparamagnetic iron oxide nanoparticles (SPION) within whole cells, by exploiting the iron oxide differential absorption contrast. Near-edge absorption soft X-ray nanotomography (NEASXT) combines whole-cell 3D structure determination at 50 nm resolution, with 3D elemental mapping and high throughput. We detected three-dimensional distribution of SPIONs within cells with 0.3 g/cm(3) sensitivity, sufficient for detecting the density corresponding to a single nanoparticle.
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