Article
Environmental Sciences
Diyang Qiu, Congyi Zhu, Ruiyi Fan, Genlin Mao, Pingzhi Wu, Jiwu Zeng
Summary: This study investigated the effects of arsenic on citric acid accumulation in citrus fruits and discovered the mechanisms underlying arsenic action. The results showed that arsenic inhibited citric acid accumulation by downregulating the transcription levels of acid regulator genes. In addition, oxidative stress signaling may play a role in the downregulation of these genes. Mitochondrial thiol proteins could be the main targets of arsenic action in citrus fruits.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Plant Sciences
Shengchao Liu, Xincheng Liu, Bangrui Gou, Dengliang Wang, Chunrong Liu, Jun Sun, Xueren Yin, Donald Grierson, Shaojia Li, Kunsong Chen
Summary: Citric acid plays important roles in plants and affects physiological processes and fruit quality. The study characterizes a transporter, CitALMT, which regulates citrate concentration in citrus fruits.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Horticulture
Shariq Mahmood Alam, Dong-Hai Liu, Yong-Zhong Liu, Han Han, Syed Bilal Hussain, Muhammad Ateeq
Summary: This study identified significant differences in organic acid profiles between top-canopy and bottom-canopy fruits, with the latter showing higher citrate content and lower malate content. The transcript levels of genes related to organic acid metabolism were also found to be different between the two canopy positions, likely due to microclimate changes impacting gene expression.
SCIENTIA HORTICULTURAE
(2022)
Article
Biochemistry & Molecular Biology
Jianqing Yu, Nuozhou Wang, Zhongqin Gong, Liping Liu, Shengli Yang, George Gong Chen, Paul Bo San Lai
Summary: The study revealed that CYP1A2 can sensitize sorafenib-resistant cells to the drug by inhibiting the NF-kappa B p65 axis, thereby increasing drug sensitivity and enhancing the inhibitory effect of the drug.
Article
Plant Sciences
Jianhui Wang, Rui Xu, Shuangping Qiu, Weichun Wang, Fan Zheng
Summary: In this study, a homologous gene of AtTT8 was identified in blood orange (Citrus sinensis cv 'Tarocco') fruit. The mRNA levels of TT8 were significantly higher in 'Tarocco' fruit than in mutant fruit lacking pigment. An alternative splicing transcript, Delta 15-TT8, was also found and its mRNA levels were higher in mutant fruit. The study demonstrated the role of TT8 in regulating anthocyanin biosynthesis and its transport, while the dysfunctional Delta 15-TT8 may act as a negative feedback to downregulate TT8 expression and limit anthocyanin accumulation.
HORTICULTURE RESEARCH
(2023)
Article
Plant Sciences
Meng-Qi Feng, Meng-Di Lu, Jian-Mei Long, Zhao-Ping Yin, Nan Jiang, Peng-Bo Wang, Yue Liu, Wen-Wu Guo, Xiao-Meng Wu
Summary: This study reveals the regulatory pathway of miR156-SPLs and miR172-TOEs in somatic embryogenesis (SE) in citrus callus. CsAGL15 and CsFUS3 activate csi-miR156a, which represses CsSPL genes and further regulates csi-miR172d and CsTOEs. This leads to alterations in starch accumulation in callus cells and affects SE efficiency in citrus.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Plant Sciences
Jiaxian He, Yuantao Xu, Ding Huang, Jialing Fu, Ziang Liu, Lun Wang, Yin Zhang, Rangwei Xu, Li Li, Xiuxin Deng, Qiang Xu
Summary: The citrus transcription factor TRIPTYCHON-LIKE plays a crucial role in regulating fruit flavor and color by competing with MYB activators. It forms a regulatory loop that balances the accumulation of citric acid and proanthocyanidins. This study demonstrates that CitTRL acts as a repressor of citric acid, proanthocyanidins, and anthocyanins accumulation through a cross-regulation mechanism.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Plant Sciences
Jiaming Li, Rongxiang Zhu, Mingyue Zhang, Beibei Cao, Xiaolong Li, Bobo Song, Zhongchi Liu, Jun Wu
Summary: Soluble sugars are important for plant growth, development, and fruit quality. A calcium-dependent protein kinase called PbCPK28 was identified in pears, and a single nucleotide polymorphism (SNP) in its regulatory region was found to be associated with diversity in fructose content. PbCPK28 was shown to interact with and phosphorylate proteins involved in sugar import into vacuoles, allowing for the genetic improvement of fruit sugar contents in pears.
Review
Biochemistry & Molecular Biology
Katarzyna Kabala, Malgorzata Janicka
Summary: There are two ATP-dependent proton pumps in plant cells, the Plasma membrane H+-ATPase (PM H+-ATPase) and the vacuolar H+-ATPase (V-ATPase). While the PM H+-ATPase transfers protons from the cytoplasm to the apoplast, the V-ATPase pumps protons into the organelle lumen. These enzymes belong to different protein families and have distinct structures and mechanisms of action. However, they can be regulated by the same mechanisms and may coordinate in certain processes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
Rasaq Olajide Akinsola, Choon Weng Lee, Edmund Ui Hang Sim, Kumaran Narayanan
Summary: Inhibition of lysosomal V-ATPase enhances the efficiency of E. coli for gene delivery into mammalian cells, as shown by the increase in green fluorescent protein expression in various cell lines.
ANALYTICAL BIOCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Nan Xiang, Yihan Zhao, Bing Zhang, Qiuming Gu, Weiling Chen, Xinbo Guo
Summary: In this study, the volatile profiles during young pomelo fruits development were investigated, and the prominent volatile components were identified. The regulatory roles of transcription factors on volatiles accumulation in young pomelos were also explored. The research findings contribute to the extraction of profitable volatiles and enrich the comprehensive utilization of young pomelos.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Genetics & Heredity
Yue Huang, Jiaxian He, Yuantao Xu, Weikang Zheng, Shaohua Wang, Peng Chen, Bin Zeng, Shuizhi Yang, Xiaolin Jiang, Zishuang Liu, Lun Wang, Xia Wang, Shengjun Liu, Zhihao Lu, Ziang Liu, Huiwen Yu, Jianqiang Yue, Junyan Gao, Xianyan Zhou, Chunrui Long, Xiuli Zeng, Yong-Jie Guo, Wen-Fu Zhang, Zongzhou Xie, Chunlong Li, Zhaocheng Ma, Wenbiao Jiao, Fei Zhang, Robert M. Larkin, Robert R. Krueger, Malcolm W. Smith, Ray Ming, Xiuxin Deng, Qiang Xu
Summary: De novo genome assemblies of 12 species from Aurantioideae and pangenome analyses provide insights into the origin and evolution of the orange subfamily and the genetic basis of flavor in citrus fruits.
Article
Biochemistry & Molecular Biology
Guihong Liang, Haixing Song, Yan Xiao, Zhenhua Zhang
Summary: This study explored the impact of V-ATPase activity in plant vacuoles on growth and development. The lack of two tonoplast-localized subunits of V-ATPase resulted in defective growth and excessive ammonium accumulation in plants, which could be alleviated by potassium supplementation. This research provided new insights into the relationship between V-ATPase, growth, and ammonium accumulation, while highlighting the role of potassium in mitigating ammonium toxicity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Cai-Yun Shi, Syed Bilal Hussain, Han Han, Shariq Mahmood Alam, Dong Liu, Yong-Zhong Liu
Summary: The low accumulation of citrate in citrus leaves is mainly due to the low expression level of the CsPH8 gene, while the high expression of citrate-utilizing genes in leaves prevents citrate accumulation.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Plant Sciences
Suwen Lu, Junli Ye, Kaijie Zhu, Yin Zhang, Mengwei Zhang, Qiang Xu, Xiuxin Deng
Summary: The study revealed that a MADS gene CsMADS5 in citrus positively regulates carotenoid biosynthesis by directly activating carotenogenic genes, leading to enhanced fruit coloration and carotenoid concentrations. Additionally, CsMADS5 physically interacts with another positive regulator CsMADS6, suggesting a collaborative role in promoting carotenoid accumulation. These findings expand our knowledge of the complex transcriptional regulatory network governing carotenoid biosynthesis during fruit ripening.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Plant Sciences
Aidi Zhang, Qiuyun Zhang, Jianzhao Li, Hansheng Gong, Xinguang Fan, Yanqing Yang, Xiaofen Liu, Xueren Yin
Article
Plant Sciences
Jing Zhang, Xue-Ren Yin, Heng Li, Meng Xu, Meng-Xue Zhang, Shao-Jia Li, Xiao-Fen Liu, Yan-Na Shi, Donald Grierson, Kun-Song Chen
JOURNAL OF EXPERIMENTAL BOTANY
(2020)
Article
Plant Sciences
Ping Xu, Hui Su, Shiqi Zhao, Rong Jin, Haiyan Cheng, Anan Xu, Wanyi Lai, Xueren Yin, Yuefei Wang
Article
Biotechnology & Applied Microbiology
Zuying Zhang, Yanna Shi, Yuchen Ma, Xiaofang Yang, Xueren Yin, Yuanyuan Zhang, Yuwei Xiao, Wenli Liu, Yunduan Li, Shaojia Li, Xiaofen Liu, Donald Grierson, Andrew C. Allan, Guihua Jiang, Kunsong Chen
PLANT BIOTECHNOLOGY JOURNAL
(2020)
Article
Agronomy
Mei-yan Tu, Ying-ying Wu, Jing Li, Dong Chen, Guo-liang Jiang, Hai-yan Song, Xue-ren Yin, Xiao-fen Liu, Ming-zhang Li, Shu-xia Sun
Summary: This study found that the change in flesh color of yellow-fleshed kiwifruit is highly correlated with chlorophyll degradation-related genes, especially three chlorophylla/b-binding protein (CAB) genes and one stay-green gene (AcSGR1), while no correlation was found with carotenoid-related genes. The results suggest the important role of chlorophyll degradation in the coloration of 'Jinshi NO.1' and provide potential candidates for further functional characterization and transcriptional regulation.
NEW ZEALAND JOURNAL OF CROP AND HORTICULTURAL SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Chenning Zhao, Xiaojuan Liu, Qin Gong, Jinping Cao, Wanxia Shen, Xueren Yin, Donald Grierson, Bo Zhang, Changjie Xu, Xian Li, Kunsong Chen, Chongde Sun
Summary: CitCHIL1 enhances the accumulation of citrus flavanones and flavones by assisting the cyclization of naringenin chalcone. The transcription factors CitERF32, CitERF33, and CitRAV1 positively regulate the expression of CitCHIL1, forming a complex that enhances flavonoid production. This study provides insight into the molecular mechanisms underlying efficient flavonoid biosynthesis.
PLANT BIOTECHNOLOGY JOURNAL
(2021)
Article
Chemistry, Applied
Yanpei Chen, Xiaocheng Zhang, Zisheng Luo, Jian Sun, Li Li, Xueren Yin, Jiangkuo Li, Yanqun Xu
Summary: The study revealed that microwave treatments can effectively delay the softening of persimmon fruit and improve fruit quality, making it a suitable method for fruit preservation.
Article
Agriculture, Multidisciplinary
Su-mei Kou, Rong Jin, Ying-ying Wu, Jing-wen Huang, Qiu-yun Zhang, Ning-jing Sun, Yong Yang, Chang-fei Guan, Wen-qiu Wang, Chang-qing Zhu, Qing-gang Zhu, Xue-ren Yin
Summary: The study found that different persimmon cultivars exhibit varying rates of deastringency under high-CO2 treatment, influenced by metabolites such as acetaldehyde and ethanol. Differentially expressed structural genes in the acetaldehyde metabolism pathway are positively correlated with acetaldehyde production.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Plant Sciences
Hang Ge, Yan-na Shi, Meng-xue Zhang, Xian Li, Xue-ren Yin, Kun-song Chen
Summary: The study identified a MADS-box gene, EjAGL65, which is involved in the transcriptional regulation of lignin biosynthesis during postharvest chilling induced flesh lignification in loquat fruit. This finding contrasts with the classical model of lignin accumulation in developmental processes and adds to our understanding of the regulatory network in fruit postharvest chilling injury.
FRONTIERS IN PLANT SCIENCE
(2021)
Editorial Material
Agronomy
Yunliu Zeng, Xueren Yin
NEW ZEALAND JOURNAL OF CROP AND HORTICULTURAL SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Mijing Jin, Sufu Gan, Jianqing Jiao, Yiheng He, Hui Liu, Xueren Yin, Qinggang Zhu, Jingping Rao
Summary: Chilling injury is a barrier to kiwifruit refrigeration, but understanding the molecular basis of the cold response in refrigerated kiwifruit is crucial. The study identified 81 bZIP family proteins in kiwifruit and revealed that the expression of AREB/ABF family members is induced by low temperature and ABA. Ectopic expression of AchnABF1 enhances cold tolerance in plants and is involved in osmotic stress response.
Review
Plant Sciences
Mareike Jezek, Andrew C. Allan, Jeffrey J. Jones, Christoph-Martin Geilfus
Summary: Foliar anthocyanin accumulation is not only induced by nitrogen or phosphorous deficiency but also by other nutritional imbalances. This misconception has led to excessive use of fertilizers, causing environmental burden. Anthocyanins have various physiological functions, and this article discusses their synthesis pathways and functions in nutrient-stressed leaves. Integrating knowledge from genetics, molecular biology, ecophysiology, and plant nutrition, the mechanisms and reasons for foliar anthocyanin accumulation under nutritional stress are deduced. Further research can utilize this knowledge to use leaf pigments as bioindicators for demand-oriented fertilizer application, benefiting the environment in the face of increasing climate crisis impacts on crop performance.
Article
Plant Sciences
Yun Zhao, Juanli Sun, Sylvia Cherono, Jian-Ping An, Andrew C. Allan, Yuepeng Han
Summary: Anthocyanin is a vital indicator for both fruit nutritional and commercial value, and its accumulation is regulated by multiple networks associated with genetic, developmental, hormonal, and environmental factors. Transcriptional and epigenetic regulation play crucial roles in anthocyanin biosynthesis. This article reviews the current understanding of the regulatory mechanisms of anthocyanin accumulation, with a focus on transcriptional and epigenetic regulation and the crosstalk between various signaling pathways. It also discusses the synergistic or antagonistic effects of developmental, hormonal, and environmental cues on anthocyanin accumulation in fruit. Recent research has significantly advanced our knowledge of the regulation of developmental cues, hormones, environmental factors, and their crosstalk in fruit anthocyanin accumulation.
Meeting Abstract
Plant Sciences
M. R. Boase, T. McGhie, L. Wang, H. Zhang, H. Luo, I. King, D. J. Lafferty, N. W. Albert, A. C. Allan, R. V. Espley
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
(2022)
Article
Horticulture
Siti Khadijah A. Karim, Andrew C. Allan, Robert J. Schaffer, Karine M. David
Summary: This study investigated the role of cell division and cell expansion in determining the final size of apple fruits, and found that both processes play important roles in determining fruit size. Different apple cultivars showed similarities and differences in gene expression and cell size. Additionally, cell sizes changed during fruit development but remained constant after a certain stage.
