Article
Plant Sciences
Coralie Chuberre, Philippe Chan, Marie-Laure Walet-Balieu, Francois Thiebert, Carole Burel, Julie Hardouin, Bruno Gugi, Muriel Bardor
Summary: This study conducted a comparative proteomic analysis of three different morphotypes of Phaeodactylum tricornutum. The results revealed significant differences in certain biological processes between oval and triradiate cells compared to fusiform cells, and the oval cells showed a distinct metabolism.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Eleonora Curcuraci, Simona Manuguerra, Concetta Maria Messina, Rosaria Arena, Giuseppe Renda, Theodora Ioannou, Vito Amato, Claire Hellio, Francisco J. Barba, Andrea Santulli
Summary: In this study, the growth performance, lipid productivity, fatty acid composition, and antioxidant content of P. tricornutum under different nitrogen conditions were investigated. It was found that nitrogen deprivation increased lipid production, decreased secondary metabolite production, upregulated genes related to fatty acid biosynthesis, and downregulated genes related to photosynthesis in P. tricornutum.
Article
Plant Sciences
Yogesh Taparia, Achintya Kumar Dolui, Sammy Boussiba, Inna Khozin-Goldberg
Summary: This study describes the construction of a modular two-component transcriptional unit system to express SpCas9 in a diatom. The system enables multiplexed targeting and marker-free genome editing. The editing efficiency of the system was evaluated by targeting the StLDP gene, and the results showed the importance of StLDP in lipid droplet regulation.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemical Research Methods
Josselin Lupette, Marianne Tardif, Sabine Brugiere, Yohann Coute, Juliette Salvaing, Eric Marechal
Summary: This study evaluated whole proteome changes in diatoms under different nitrogen conditions, finding that nitrogen limitation can lead to the arrest of amino acid and protein synthesis, remodeling of nitrogen metabolism, and a decrease in proteasome abundance.
Article
Biotechnology & Applied Microbiology
Andrew Diamond, Aracely Maribel Diaz-Garza, Jessica Li, Samuel S. Slattery, Natacha Merindol, Elisa Fantino, Fatma Meddeb-Mouelhi, Bogumil J. Karas, Simon Barnabe, Isabel Desgagne-Penix
Summary: The efficacy and limitations of the episome expression system in Phaeodactylum tricornutum were studied, revealing issues such as episome rearrangement and toxicity of fusion proteins.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2023)
Article
Green & Sustainable Science & Technology
Yixi Su, Maonian Xu, Sigurour Brynjolfsson, Weiqi Fu
Summary: This study investigated the adaptive responses of the marine diatom Phaeodactylum tricornutum to acidic stress. The evolved strains showed increased growth rate in acidic conditions, and phenotypic characterization demonstrated stable acid tolerance. RNA sequencing and whole-genome re-sequencing analyses revealed upregulation of core pathways, including photosynthesis and pH regulation/ion transport, across all three evolved strains. This study demonstrated the feasibility of recovering photosynthetic capability and provided molecular data to reveal essential alterations in genetic regulations for acid tolerance.
JOURNAL OF CLEANER PRODUCTION
(2023)
Article
Plant Sciences
Teng Huang, Yufang Pan, Eric Marechal, Hanhua Hu
Summary: By using proteomic technology, this study identified 2850 proteins, including 92 plastid-encoded proteins, in the Phaeodactylum tricornutum plastid, and determined 839 nuclear-encoded proteins to be plastidial proteins. The study also revealed the complex nature of the diatom plastid and its involvement in various metabolic processes, such as triacylglycerol hydrolysis, glycolysis, and synthesis of tocopherol and triterpenes.
Article
Plant Sciences
Victor Murison, Josiane Herault, Martine Come, Sabrina Guinio, Alexis Lebon, Christophe Chamot, Magalie Benard, Ludovic Galas, Benoit Schoefs, Justine Marchand, Muriel Bardor, Lionel Ulmann
Summary: This study investigated the accumulation and degradation of lipid droplets in two ecotypes of P. tricornutum under nitrogen deprivation and resupply conditions. The results showed that lipid accumulation may be limited by the size of the plastid, and a possible migration of lipid droplets to vacuoles was observed, suggesting autophagic degradation.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Limnology
Peipei Zhao, Qinghua Wu, Xuekui Xia, Shiyi Guo, Sizhong Shen, Yujue Wang, Aiyou Huang
Summary: Diatoms, such as Phaeodactylum tricornutum, play a crucial role in global net primary productivity and biogeochemical cycles. This study investigated the responses of P. tricornutum to low oxygen conditions and found that it can reset its metabolism pathways and activate stress resistance mechanisms to adapt to hypoxic environments.
JOURNAL OF OCEANOLOGY AND LIMNOLOGY
(2022)
Article
Plant Sciences
Luisana Avilan, Regine Lebrun, Carine Puppo, Sylvie Citerne, Stephane Cuine, Yonghua Li-Beisson, Benoit Menand, Ben Field, Brigitte Gontero
Summary: This study reveals the function of ppGpp in the diatom Phaeodactylum tricornutum and demonstrates its important roles in photosynthesis, growth, and protein protection response.
Article
Plant Sciences
Richard Smith, Juliette Jouhet, Chiara Gandini, Vladimir Nekrasov, Eric Marechal, Johnathan A. Napier, Olga Sayanova
Summary: The marine diatom Phaeodactylum tricornutum is a model organism for studying long chain polyunsaturated fatty acids biosynthesis. The study identified the key enzyme plastidial acyl carrier protein Delta 9-desaturase and revealed its important role in EPA synthesis and triacylglycerol production. This research provides insight into lipid metabolism in diatoms and suggests novel approaches for manipulating oil composition in microalgae.
Article
Plant Sciences
Matteo Scarsini, Adrien Thurotte, Brigitte Veidl, Frederic Amiard, Frederick Niepceron, Myriam Badawi, Fabienne Lagarde, Benoit Schoefs, Justine Marchand
Summary: A study on the cellular quota quantification method of the diatom Phaeodactylum tricornutum showed significant differences in lipid metabolism regulation between different ecotypes of the diatom.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Environmental Sciences
Jie Ma, Beibei Zhou, Fengyuan Chen, Ke Pan
Summary: Metal tolerance in marine diatoms varies between morphotypes, where the oval morphotype of Phaeodactylum tricornutum accumulates more cadmium (Cd) but is also more tolerant to Cd due to its smaller spherical form, rougher cell surface, lower surface potential, lower permeability to Cd ions, and higher levels of phytochelatin and glutathione. This study provides new insights into the adaptive strategies of diatoms to changing environments, contributing to their evolutionary success.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Green & Sustainable Science & Technology
Yi Cui, Skye R. Thomas-Hall, Elvis T. Chua, Peer M. Schenk
Summary: A biorefinery platform for cost-effective cultivation, harvest, and processing of Phaeodactylum tricornutum has been developed to extract omega-3 fatty acids and protein. Land-based cultivation of P. tricornutum is shown to be a sustainable source of omega-3 fatty acids without pressure on marine bioresources, with ethanol identified as the safest and most suitable option for biorefinery.
JOURNAL OF CLEANER PRODUCTION
(2021)
Article
Biotechnology & Applied Microbiology
Aiyou Huang, Yuanxiang Li, Jiawen Duan, Shiyi Guo, Xiaoni Cai, Xiang Zhang, Hao Long, Wei Ren, Zhenyu Xie
Summary: This study found that lactate plays important roles in energy homeostasis and C:N balance regulation in Phaeodactylum tricornutum through protein lactylation modification in response to low nitrogen and high CO2 conditions.
BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS
(2022)
Article
Environmental Sciences
Erkui Yue, Fuxi Rong, Zhen Liu, Songlin Ruan, Tao Lu, Haifeng Qian
Summary: This study identifies miR535 as a key regulator of Cd tolerance and accumulation in rice plants. Knocking out miR535 increases Cd tolerance, while overexpressing miR535 has the opposite effect. Cd accumulation is significantly higher in OE535 plants and lower in mir535 plants compared to wild type. The CRISPR/Cas9 technology may be used to create new rice varieties for food safety.
JOURNAL OF ENVIRONMENTAL SCIENCES
(2023)
Article
Environmental Sciences
Yitian Yu, Qi Zhang, Zhenyan Zhang, Shuyidan Zhou, Mingkang Jin, Dong Zhu, Xiaoru Yang, Haifeng Qian, Tao Lu
Summary: Knowledge of the dissemination and emergence of antibiotic resistance genes (ARGs) in the plant rhizosphere is important for evaluating the risk they pose to soil planetary health. In this study, we analyzed the changes in the rhizosphere antibiotic resistome during the enrichment process of the rhizosphere microbiome in Arabidopsis thaliana. We found that plants selectively enriched beneficial bacteria with many ARGs, and the wheat rhizosphere had a higher abundance of ARGs compared to other crops. These findings highlight the potential threat to human health from the dissemination of ARGs, despite their beneficial functions in the plant rhizosphere.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Environmental Sciences
Fan Zhang, Nuohan Xu, Zhenyan Zhang, Qi Zhang, Yaohui Yang, Zhitao Yu, Liwei Sun, Tao Lu, Haifeng Qian
Summary: The rhizosphere microbiome is crucial for plant growth and acts as an important interface for resource exchange between plants and the soil environment. This study analyzed the variations of bacterial and fungal communities in the rhizosphere of four crop species during successive cultivation and found that specific microorganisms that benefit plant growth decreased in abundance over time, while potentially pathogenic fungi increased. The co-occurrence network analysis showed that bacterial and fungal communities under maize were more stable than those under other crops. The findings highlight the importance of indigenous soil flora in determining plant growth and suggest the potential use of critical microorganisms to control crop growth and yield.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Marine & Freshwater Biology
Yan Wang, Kepin Ni, Zhenyan Zhang, Nuohan Xu, Chaotang Lei, Bingfeng Chen, Qi Zhang, Liwei Sun, Yiling Chen, Tao Lu, Haifeng Qian
Summary: The emergence and transmission of antibiotic resistance genes (ARGs) and virulence factors (VFs) pose health risks to the ecosystem and humans. Understanding how non-antibiotic antimicrobial agents drive the expression of ARGs and VFs in freshwater ecosystems, however, remains large challenges.
AQUATIC TOXICOLOGY
(2023)
Article
Environmental Sciences
Guoyan Qin, Qi Zhang, Ziyao Zhang, Yiling Chen, Jichao Zhu, Yaohui Yang, W. J. G. M. Peijnenburg, Haifeng Qian
Summary: In this study, the impact of the fungicide difenoconazole on the gut microbial community of Enchytraeus crypticus and the soil microbiome was investigated. The results showed that difenoconazole treatment reduced body weight and increased oxidative stress levels in E. crypticus. The fungicide also altered the composition and structure of the gut microbial community and impaired the abundance of beneficial bacteria in the soil, affecting the stability of the soil-soil fauna microecology. Additionally, the study revealed the enrichment of bacterial genes involved in detoxification and virus genes involved in the carbon cycle, showing their ecological importance under pesticide stress.
ENVIRONMENTAL POLLUTION
(2023)
Article
Engineering, Environmental
Bingfeng Chen, Zhenyan Zhang, Qi Zhang, Nuohan Xu, Tao Lu, Tingzhang Wang, Wenjie Hong, Zhengwei Fu, Josep Penuelas, Michael Gillings, Haifeng Qian
Summary: Antimicrobial peptides are a promising class of antimicrobials that can combat antibiotic resistance crisis. A global study on the diversity, hosts, and resistance potential of these peptides is lacking. This research analyzed 52,515 metagenome-assembled genomes to investigate the diversity and number of antimicrobial peptides in different habitats. The number of peptides was highest in the human gut microbiome, and their hosts varied across habitats. The risk of resistance genes to human health was quantified, with those conferring resistance to multiple AMPs and polymyxin B posing the highest threat.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Environmental Sciences
Wei Lin, Zhenyan Zhang, Yiling Chen, Qi Zhang, Mingjing Ke, Tao Lu, Haifeng Qian
Summary: This study investigated the effects of glyphosate on six common cyanobacterial species and found that the growth of five species was promoted to different degrees, while only Pseudanabaena was inhibited. Phylogenetic analysis revealed that Pseudanabaena, which is more closely related to plants, was sensitive to glyphosate. Additionally, the study demonstrated that glyphosate served as a source of phosphorus and accelerated cyanobacterial growth.
JOURNAL OF ENVIRONMENTAL SCIENCES
(2023)
Article
Engineering, Environmental
Ming-Kang Jin, Qi Zhang, Yu-Tian Yang, Cai-Xia Zhao, Jian Li, Hongjie Li, Haifeng Qian, Dong Zhu, Yong-Guan Zhu
Summary: Worldwide, the use of pyrethroids, such as cypermethrin, is widespread as insecticides, but their impact on the soil microbiome and non-target soil fauna is not well understood. In this study, we investigated the effects of cypermethrin on the bacterial communities and antibiotic resistance genes (ARGs) in the soil and gut of Enchytraeus crypticus using genetic sequencing and qPCR. The results showed that cypermethrin exposure led to an increase in potential pathogens in the soil and gut microbiome of E. crypticus, disrupting the microbiome structure and immune system. The co-occurrence of potential pathogens, ARGs, and mobile genetic elements revealed an increased risk of pathogenicity and antibiotic resistance. Structural equation modeling demonstrated that ARG dissemination was promoted by both mobile genetic elements and the ratio of core to non-core bacterial abundance. Overall, this study provides important insights into the environmental risk of cypermethrin on the dissemination of ARGs in the soil and non-target soil fauna.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Engineering, Environmental
Bingfeng Chen, Zhenyan Zhang, Tingzhang Wang, Hang Hu, Guoyan Qin, Tao Lu, Wenjie Hong, Jun Hu, Josep Penuelas, Haifeng Qian
Summary: We created a global map using a machine-learning model to predict marine microplastic pollution. The results showed that microplastics accumulate in subtropical gyres and near polar seas. Our study also identified areas with high microplastic pollution and high potential for biodegradation, but the limited number of samples hindered accurate predictions.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Environmental Sciences
Nuohan Xu, Hang Hu, Yan Wang, Zhenyan Zhang, Qi Zhang, Mingjing Ke, Tao Lu, Josep Penuelas, Haifeng Qian
Summary: River microbiotas play a critical role in geochemical processes and ecological functions of rivers, but their response to environmental drivers is sensitive. Understanding the geographic patterns of river microbial traits can provide important insights into river health and function.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Ecology
Tao Lu, Nuohan Xu, Chaotang Lei, Qi Zhang, Zhenyan Zhang, Liwei Sun, Feng He, Ning-Yi Zhou, Josep Penuelas, Yong-Guan Zhu, Haifeng Qian
Summary: In this study, 6102 high-quality sequencing results of soil bacterial samples were re-analyzed. The results showed that the type of land use was the principal driver of bacterial richness and diversity. Additionally, SOC content was found to be positively correlated with key bacteria and total nitrogen content.
SOIL ECOLOGY LETTERS
(2023)
Article
Agriculture, Dairy & Animal Science
Chunlei Yang, Xiangfei Deng, Peter Lund, Haixia Liu, Xingwang Ding, Zhengwei Fu, Naifeng Zhang, Jinjun Li, Lifeng Dong
Summary: Developing alternatives to antibiotics for prevention of gastrointestinal dysbiosis in early-weaning farmed animals is urgently needed. This study explored the potential effects of trans-10, cis-12 conjugated linoleic acid (CLA) on maintaining ruminal homeostasis of young ruminants during the weaning transition period. Results showed that CLA supplementation enhanced the ruminal microbiota-driven transcriptional regulation and alleviated early-weaning stress in young ruminants.
Article
Environmental Sciences
Chaotang Lei, Tao Lu, Haifeng Qian, Yuxue Liu
Summary: Biochar amendment significantly affects the soil microbial community by altering the composition of bacteria and fungi. It enhances the stability of the soil microbial community while reducing the interactions between fungi and bacteria. In addition, biochar amendment enhances the biosynthetic capabilities of bacteria and increases the fungal nutrient patterns, resulting in an increase in the abundance and diversity of saprophytic fungi that promote soil nutrient cycling.
Article
Ecology
Yitian Yu, Qi Zhang, Zhenyan Zhang, Nuohan Xu, Yan Li, Mingkang Jin, Guoqiang Feng, Haifeng Qian, Tao Lu
Summary: This study investigated the impact of residual chlorine on soil microbial communities. The results showed that continuous chlorine treatment did not have a lasting effect on the diversity and composition of soil microbial communities, and slightly affected the metabolic functions of rhizosphere microbial communities, which later recovered to their original status. Due to the potential slight long-term effects on plant growth and soil micro-ecology, conducting environmental risk assessments and minimizing risks to the environment are crucial.
SOIL ECOLOGY LETTERS
(2023)
Article
Materials Science, Biomaterials
Qinglian Hu, Chao He, Zhuoting Lu, Ying He, Hui Xie, Jingyu Li, Zhengwei Fu, Bing Guo
Summary: Sub-cellular targeted mild photothermal therapy (PTT) has the potential to improve therapeutic efficacy and minimize damage to healthy tissues. Mitochondria-targeted nanoparticles (M-TD1 NPs) constructed using small organic molecules (TD1) exhibit excellent photothermal performance, leading to apoptosis and autophagy of cancer cells, thus achieving antitumor and metastasis inhibition effects.
BIOMATERIALS SCIENCE
(2022)