Article
Multidisciplinary Sciences
Bo Jiang, Xuan Wu, Fang Meng, Limiao Si, Sunrun Cao, Yuqing Dong, Huayi Sun, Mengzhu Lv, Hongde Xu, Ning Bai, Qiqiang Guo, Xiaoyu Song, Yang Yu, Wendong Guo, Fei Yi, Tingting Zhou, Xiaoman Li, Yanling Feng, Zhuo Wang, Dan Zhang, Yi Guan, Mengtao Ma, Jingwei Liu, Xining Li, Weidong Zhao, Baohua Liu, Toren Finkel, Liu Cao
Summary: Progerin, a result of LMNA mutation, causes nuclear abnormalities and premature aging. Impaired IGF-1R/Akt signaling pathway leads to growth retardation and aging. Progerin mislocalizes outside of nucleus, interacts with IGF-1R, and down-regulates its expression, resulting in inhibited mitochondrial respiration, retarded cell growth, and accelerated senescence. PTEN inhibitor bpV (HOpic) increases Akt activity and improves abnormalities in progeria. These findings provide important insights into the role of Progerin in regulating IGF-1R/Akt signaling and have potential implications in treating LMNA-associated progeroid disorders.
Article
Cell Biology
Yu-Tzu Chan, Ruey-Jen Lin, Ya-Hui Wang, Tsai-Hsien Hung, Yenlin Huang, John Yu, Jyh-Cherng Yu, Alice L. Yu
Summary: This study reveals the crucial role of YAP in breast cancer stem cells. Knockdown of YAP reduces the viability and stemness of BCSCs in vitro and tumor formation in vivo. IGF-1R and IGF-1 regulate the expression and localization of YAP, and YAP overexpression increases the expression of IGF-1. Clinical analysis shows that high levels of YAP and IGF-1 are associated with shorter overall survival in basal-like breast cancer patients. In conclusion, the interplay between YAP and the IGF-1/IGF-1R pathway is clinically relevant for sustaining the properties of BCSCs.
CELL COMMUNICATION AND SIGNALING
(2023)
Article
Biotechnology & Applied Microbiology
Runze Zhang, Yanhong Lian, Kangjie Xie, Yunfang Cai, Yafei Pan, Yuntian Zhu
Summary: Ropivacaine inhibits proliferation, promotes apoptosis, suppresses migration and invasion, and targets the IGF-1R/PI3K/AKT/mTOR signaling pathway to suppress hepatocellular carcinoma progression.
Article
Biochemistry & Molecular Biology
Qin Zhao, Hong Su, Wei Jiang, Haodong Luo, Lu Pan, Yuan Liu, Ce Yang, Ying Yin, Lehua Yu, Botao Tan
Summary: This study investigated the effect of IGF-1 combined with OPN on neuronal axon growth and protein expression levels, and its underlying mechanism. The results showed that IGF-1 combined with OPN promoted axon growth through the IGF-1R/Akt/mTOR signaling pathway in lipid rafts, and the effect was superior to either agent alone. However, this effect was suppressed by the mTOR inhibitor rapamycin or the lipid raft cholesterol extraction agent M-beta-CD. Additionally, M-beta-CD significantly decreased the expression of p-IR. Western blot analysis revealed that the IGF-1 combined with OPN group had the highest expression levels of IR and p-IR in the neuronal lipid rafts. The enrichment of IR by IGF-1 combined with OPN was weakened, and p-IR was reduced when M-beta-CD was administered to the lipid rafts of neurons. This study demonstrated that IGF-1 combined with OPN promoted axon growth by activating the IGF-1R/Akt/mTOR signaling pathway in lipid rafts.
NEUROCHEMICAL RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Hai-Ying Mo, Qi-Yao Wei, Qiu-Hua Zhong, Xiao-Yun Zhao, Dan Guo, Jin Han, Wachiraporn Noracharttiyapot, Lydia Visser, Anke van den Berg, Yan-Ming Xu, Andy T. Y. Lau
Summary: CYP27C1 expression levels vary in lung cancer cell lines. Silencing CYP27C1 enhances cell proliferation, colony formation, migration, and tolerance towards anticancer agents in lung cancer cells. This study reveals that CYP27C1 impacts lung cancer cell development by participating in the regulation of the IGF-1R/Akt/p53 signaling pathway and determines cellular sensitivity towards multiple anticancer agents.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Wenwei Wan, Qikai Qin, Linshan Xie, Hanqing Zhang, Fan Wu, Raymond C. Stevens, Yan Liu
Summary: The GLP-1R cellular signaling mechanism in the treatment of type 2 diabetes mellitus (T2DM) has been extensively studied. However, there is still a need for functional molecules with reduced side effects and enhanced therapeutic efficacy. This review summarizes the cellular signaling of GLP-1R and its involvement in T2DM treatment, and outlines the development of novel therapeutic drugs for T2DM and other metabolic diseases through incretin therapy.
Review
Cell Biology
Pei-Chin Chen, Yung-Che Kuo, Cheng-Ming Chuong, Yen-Hua Huang
Summary: Stem cells interact harmoniously with their niches during development and tumor pathology, influencing cell fate. The modulation of IGF-1R signaling plays a crucial role in determining cell fate.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Veterinary Sciences
Jie Song, Linlin Hao, Xiangfang Zeng, Rui Yang, Shiyan Qiao, Chunli Wang, Hao Yu, Siyao Wang, Yingying Jiao, Hongyao Jia, Songcai Liu, Ying Zhang
Summary: This study aimed to investigate the effects of novel miRNA Y-56 on porcine skeletal muscle satellite cells (PSCs) and found that Y-56 inhibited cell proliferation and cell cycle by targeting the IGF-1R-mediated AKT and ERK pathways.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Endocrinology & Metabolism
Alessandra Esposito, Michael Kluppel, Brittany M. Wilson, Sai R. K. Meka, Anna Spagnoli
Summary: Non-union fractures have significant clinical and economic burdens, and this study reveals the importance of the interaction between CXCR4 and IGF-1R in maintaining bone homeostasis and promoting fracture repair. By regulating the IGF-1R signaling pathway and CXCR4 expression, improvements in fracture healing can be achieved, suggesting potential for the development of novel therapeutic interventions.
Article
Biochemistry & Molecular Biology
Ting Liu, Yang Li, Baoyu Yang, Haozhen Wang, Chen Lu, Alan K. Chang, Xiuting Huang, Xiujin Zhang, Ziyin Lu, Xiuli Lu, Bing Gao
Summary: The study revealed that reducing cholesterol levels can lead to neural apoptosis by impairing the IGF-1-Akt survival signaling pathway in the brain, potentially contributing to the pathogenesis of Alzheimer's disease.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Cardiac & Cardiovascular Systems
Wei Jiang, Chun Gan, Xindi Zhou, Qing Yang, Dan Chen, Han Xiao, Lujun Dai, Yaxi Chen, Mo Wang, Haiping Yang, Qiu Li
Summary: Our groundbreaking study has found that the soluble form of Klotho can effectively inhibit high glucose-induced ox-LDL deposition in podocytes affected by DKD. We further demonstrated that Klotho achieves this inhibition by reducing the expression of IGF-1R, leading to a decrease in RAC1 expression and an enhancement of mitochondrial function. Ultimately, this results in a significant reduction in OLR1 expression, thereby reducing renal ox-LDL deposition in DKD.
CARDIOVASCULAR DIABETOLOGY
(2023)
Article
Pharmacology & Pharmacy
Nyera H. El-Shafei, Mai A. Zaafan, Esraa A. Kandil, Rabab H. Sayed
Summary: Benign prostatic hyperplasia (BPH) is a non-malignant enlargement of prostate cells that causes lower urinary tract symptoms. The pathogenesis of BPH involves various factors such as androgens receptors signaling pathways, oxidative stress, apoptosis, and potential changes in IGF-1/PI3K/AKT/FOXO pathway. This study investigates the potential beneficial effects of simvastatin on testosterone-induced BPH.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Li Chen, Meng Tian, Jing Yang, Zhenxu Wu
Summary: Polymer microspheres have shown great potential in bone tissue engineering due to their unique properties. In this study, BBR-encapsulated IGF-1@PLGA/HA composite microspheres were fabricated and shown to promote osteogenic differentiation of bone cells, indicating their potential for bone regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Xiaofu Yu, Ruoying Mao, Wei Feng, Yazhen Zhao, Jing Qin, Yunshan Yang, Ansheng Wang, Zhong Shi
Summary: Esophageal squamous cell carcinoma (ESCC) is a major health problem, especially in the Chinese population. WISP3 expression is significantly downregulated in ESCC tissues, correlating with worse overall survival of ESCC patients. WISP3 acts as a tumor suppressor in ESCC by inhibiting AKT activity via the IGF-2-IGF1R signaling cascade.
EXPERIMENTAL CELL RESEARCH
(2021)
Article
Medicine, Research & Experimental
Jiangtao Yu, Xiaoli Hu, Xiuxiu Chen, Qiangyong Zhou, Qi Jiang, Zhengzheng Shi, Haiyan Zhu
Summary: CNOT7 plays an important role in biological processes and has potential for treating malignant tumors. This study aimed to investigate the functions and mechanism of CNOT7 in ovarian cancer. Results showed that CNOT7 overexpression in A2780 and SKOV3 cells could be partially or completely eliminated by treatment with the AKT inhibitor LY294002.
Article
Biochemistry & Molecular Biology
Ajit C. Dhadve, Kishore Hari, Bharat Rekhi, Mohit Kumar Jolly, Abhijit De, Pritha Ray
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2020)
Article
Cell Biology
Arijit Mal, Amirali B. Bukhari, Ram K. Singh, Aastha Kapoor, Amlan Barai, Ishan Deshpande, Tabassum Wadasadawala, Pritha Ray, Shamik Sen, Abhijit De
Summary: A substantial number of breast cancer patients undergoing radiation therapy develop local recurrence over time, suggesting adaptive radioresistance. This study established radioresistant cell lines and found that EpCAM overexpression in CSCs contributes to radioresistance and metastatic behavior. EpCAM-overexpressing cells exhibit plasticity and heterogeneity, ultimately aggravating the disease condition by inducing radioresistant and metastatic behavior.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Pharmacology & Pharmacy
Omkar Mhatre, B. Pradeep K. Reddy, Chetna Patnaik, Sabyasachi Chakrabarty, Aravind Ingle, Abhijit De, Rohit Srivastava
Summary: Niclosamide, an FDA approved antihelminthic drug, is now being repurposed as a potent anti-cancer and anti-inflammatory agent through encapsulation in polydopamine nanoparticles. The nanoparticles exhibit high drug loading efficiency, pH-dependent drug release profile, and time-dependent cellular uptake with significant cytotoxicity in cancer cells. Furthermore, the nanoparticles show excellent in vivo compatibility and non-toxic nature, demonstrating their potential for clinical application.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Pharmacology & Pharmacy
Rutika Godse, Maitreyi Rathod, Abhijit De, Ujwala Shinde
Summary: This study developed a novel sugar receptor-targeted drug delivery system for retinoblastoma cells using etoposide as a model drug. The system showed high specificity and cytotoxicity, demonstrating potential as a new therapeutic approach for retinoblastoma.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2021)
Article
Pharmacology & Pharmacy
Aishwarya Shetty, Sumit K. Mishra, Abhijit De, Sudeshna Chandra
Summary: This study reports the synthesis of a multifunctional nanocomposite for targeted cancer therapy. The nanocomposite exhibits near-infrared light responsive photothermal therapy and potential for photoluminescence. Both in vitro and in vivo experiments demonstrate the promising application of this nanocomposite in cancer treatment.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2022)
Article
Oncology
Pranay Dey, Mansi Joshi, Aaiyas Mujawar, Renu Malhotra, Abhijit De
Summary: In this study, we demonstrated for the first time the specific and direct knockdown of important oncogenic proteins and their phospho-PTM targets using a method called 'TRIM-ing' in TRIM21 overexpressing breast cancer cells. The results showed rapid degradation of the target proteins, disrupting downstream signaling and providing significant therapeutic effects.
Article
Biochemistry & Molecular Biology
Pranay Dey, Nikhil Gadewal, Abhijit De
Summary: Among the EGFR family, HER3 acts as a pseudo-kinase and primarily interacts with HER2 in the presence of heregulin-1 beta. The hotspot mutations G284R and D297Y, as well as the double mutant HER2-S310F/HER3-G284R, were identified in breast cancer patients. These mutations cause conformational changes in the flanking regions of HER2, resulting in an unstable heterodimer and abrogating downstream signalling. The HER3-D297Y mutation forms stable interactions with EGFR-WT in the presence of EGF or heregulin-1 beta, making cancer cells susceptible to EGFR-targeted drugs.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Analytical
Aaiyas Mujawar, Pratham Phadte, Ksenia A. Palkina, Nadezhda M. Markina, Ameena Mohammad, Bhushan L. Thakur, Karen S. Sarkisyan, Anastasia V. Balakireva, Pritha Ray, Ilia Yamplosky, Abhijit De
Summary: This study presents the development of a humanized luciferase imaging reporter based on a mushroom luciferase called Luz. In vitro and in vivo assessments demonstrate that the human-codon-optimized Luz has higher activity than native Luz in cancer cell types. The importance of live cell multiplexed measurement of gene regulatory function using more than two luciferases is suggested to address realistic situations in disease biology.
Article
Materials Science, Multidisciplinary
Gayathri Ravichandran, Dinesh Harijan, Nagarajan Ganapathy, Ganesan Prabusankar, Abhijit De, Aravind Kumar Rengan
Summary: In this study, we have uncovered the potential therapeutic role of cobalt nanoparticles by elucidating their interaction with oxygen and amino acids. This discovery has led to the design of a novel combinatorial targeted therapy that induces cell death through starvation and acidification, as well as the identification of autophagy and cellular degradation.
ACS MATERIALS LETTERS
(2023)
Article
Nanoscience & Nanotechnology
K. Shitaljit Sharma, M. Swathi Raju, Suhas Phapale, Snehal K. Valvi, Akhil K. Dubey, Dibakar Goswami, Debes Ray, Abhijit De, Prasad P. Phadnis, Vinod K. Aswal, Rajesh Vatsa, Haladhar D. Sarma
Summary: Based on boron neutron capture therapy (BNCT), researchers have developed a zinc gallate-based nanoformulation for cancer treatment and diagnosis. The nanoformulation, with its functionalized surface, effectively targets tumor cells and demonstrates excellent therapeutic efficacy in in vitro and in vivo studies.
ACS APPLIED BIO MATERIALS
(2022)
Article
Nanoscience & Nanotechnology
K. Shitaljit Sharma, Swathi M. Raju, Dibakar Goswami, Abhijit De, Prasad P. Phadnis, Rajesh K. Vatsa
Summary: In this study, zinc gallate-based persistent luminescence nanoparticles coated with a variant of pH-low-insertion peptide (pHLIP) were developed for cancer detection and diagnosis. Results indicated that these nanoparticles exhibited faster and more precise uptake in the acidic tumor environment compared to other organs. Experiments on mice showed successful tumor targeting within a short period of time.
ACS APPLIED BIO MATERIALS
(2021)
Article
Nanoscience & Nanotechnology
Nishant Kumar Jain, Shalini Dimri, Rajendra Prasad, Gayathri Ravichandran, Vegi Naidu, Abhijit De, Rohit Srivastava
ACS APPLIED BIO MATERIALS
(2020)
Article
Nanoscience & Nanotechnology
Kandala Laxman, B. Pradeep K. Reddy, Sumit K. Mishra, Maddala Bala Gopal, Andrea Robinson, Abhijit De, Rohit Srivastava, Mangalampalli Ravikanth
ACS APPLIED MATERIALS & INTERFACES
(2020)
Article
Nanoscience & Nanotechnology
R. S. Prabhuraj, Arijit Mal, Snehal K. Valvi, Rohit Srivastava, Abhijit De, Rajdip Bandyopadhyaya
ACS APPLIED BIO MATERIALS
(2020)
Article
Oncology
Shalini Dimri, Renu Malhotra, Tanuja Shet, Smruti Mokal, Sudeep Gupta, Abhijit De
EXPERIMENTAL CELL RESEARCH
(2020)
