Article
Optics
N. R. Subedi, S. Yaraghi, P. S. Jung, G. Kukal, A. G. McDonald, D. N. Christodoulides, A. E. Vasdekis
Summary: Research shows that digitally scanned Airy beams can improve the throughput rates in Raman imaging by an order of magnitude compared to traditional diffraction-limited beams, achieving high contrast and 1 micron axial resolution for three-dimensional imaging of microparticles. This method also achieves orders of magnitude lower irradiation density than coherent Raman imaging schemes, with faster speed and lower phototoxicity.
Article
Developmental Biology
Mostafa Aakhte, Hans-Arno J. Mueller
Summary: Light-sheet or selective plane illumination microscopy (SPIM) is suitable for in toto imaging of living specimens at high temporal-spatial resolution, but faces limitations due to light scattering in opaque specimens. The Multiview tiling SPIM (MT-SPIM) combines the strengths of Multi-view SPIM (M-SPIM) and a confined, multi-tiled light sheet, providing high-resolution, robust, and rotation-free imaging of living specimens. It improves axial resolution by a factor of two, enhancing automated segmentation of cellular structures.
Article
Biochemical Research Methods
Kevin M. Dean, Tonmoy Chakraborty, Stephan Daetwyler, Jinlong Lin, Gerard Garrelts, Ons M'Saad, Hannahmariam T. Mekbib, Fabian F. Voigt, Martina Schaettin, Esther T. Stoeckli, Fritjof Helmchen, Joerg Bewersdorf, Reto Fiolka
Summary: The protocol provides detailed instructions for assembling and operating a versatile variant of light-sheet fluorescence microscopy called axially swept light-sheet microscopy (ASLM), which offers an unparalleled combination of field of view, optical resolution, and optical sectioning. It includes information on the working principle, applications, practical tips, part lists, schematics, and software for instrument control of ASLM.
Article
Biochemical Research Methods
Petra Paie, Gianmaria Calisesi, Alessia Candeo, Andrea Comi, Federico Sala, Francesco Ceccarelli, Ada De Luigi, Pietro Veglianese, Korbinian Muhlberger, Michael Fokine, Gianluca Valentini, Roberto Osellame, Mark Neil, Andrea Bassi, Francesca Bragheri
Summary: Heterogeneity investigation at the single-cell level is crucial in clinical research. The combination of light sheet fluorescence microscopy and structured illumination microscopy in an optofluidic platform enables high throughput super-resolution imaging, allowing comprehensive evaluation of cellular heterogeneity at high resolution.
Article
Optics
Bo Xiong, Tianyi Zhu, Yuhan Xiang, Xiaopeng Li, Jinqiang Yu, Zheng Jiang, Yihan Niu, Dong Jiang, Xu Zhang, Lu Fang, Jiamin Wu, Qionghai Dai
Summary: MiSLFM utilizes a tilted mirror to achieve super-resolved axial resolution with a single objective, expanding the depth of field of LFM. It allows observation of various organelle interactions and intercellular interactions, as well as more robust blood cell tracking in zebrafish larvae under low light conditions.
LIGHT-SCIENCE & APPLICATIONS
(2021)
Article
Cell Biology
Laura Zoe Kreplin, Senthil Arumugam
Summary: Live-cell imaging plays a crucial role in researching organelle dynamics, cytoskeletal interactions, membrane protrusions, and cell motility. In recent years, various light-sheet geometries have been developed to push the boundaries of measurements.
CURRENT OPINION IN CELL BIOLOGY
(2023)
Article
Optics
Behzad Khajavi, Ruijiao Sun, Harshdeep Singh Chawla, Henry H. Le, Manmohan Singh, Alexander W. Schill, Mary E. Dickinson, David Mayerich, Kirill Larin
Summary: A high-resolution imaging system combining OCT and ISFM was developed to enable faster and more specific imaging of mouse embryos compared to traditional fluorescence microscopy. OCT provides structural information about the embryo from the same plane illuminated by LSFM, allowing for co-registered functional and structural imaging of mouse embryos.
Article
Chemistry, Analytical
Yingying Cao, Seungah Lee, Kyungsoo Kim, Seong Ho Kang
Summary: In this study, a four-dimensional cuboid multiangle illumination-based light-sheet super-resolution (4D CMLS) imaging system was developed to overcome optical illusions in cells. The system integrated total internal reflection fluorescence and a differential interference contrast microscope, and used a rotatable cuboid prism to change the direction of light and select the optimal illumination angle. The 4D CMLS imaging method enables in-depth 4D observation of samples, making it suitable for single-molecule and subcellular organelle observations in single cells.
ANALYTICAL CHEMISTRY
(2022)
Article
Optics
Feng Xing, Xiaoming He, Kekuan Wang, Depeng Wang, Huijun Tan
Summary: A dual-view light-field particle imaging velocimetry (LF-PIV) system was proposed to achieve three-dimensional acquisition at isotropic spatial resolution with only one camera. Feasibility of the system was comprehensively assessed through imaging of sparsely distributed beads and vortex flows. Results showed that the dual-view LF-PIV system resolved beads with isotropic resolution, reported lower errors of the divergence, and revealed the three-dimensional vortex field more accurately than the conventional system. It opens up avenues for large-scale high-resolution flow dynamics imaging without additional cost.
OPTICS AND LASERS IN ENGINEERING
(2023)
Article
Biology
Soohyun Park, Myeongsu Na, Sunghoe Chang, Ki Hean Kim
Summary: The development of high-resolution open-top axially swept LSM (HR-OTAS-LSM) enables high-throughput and high-resolution imaging in all dimensions. The method uses an aberration-corrected axially swept excitation light sheet and a high numerical aperture immersion objective lens to achieve high axial and lateral resolutions.
Article
Optics
Xuanwen Hua, Wenhao Liu, Shu Jia
Summary: The study introduces a high-resolution Fourier light-field microscopy (HR-FLFM) for fast and volumetric live-cell imaging, enabling exploration of intracellular organization and processes for understanding cell physiology, development, and pathology. HR-FLFM offers near-diffraction-limited resolution in all three dimensions, an extended focal depth to several micrometers, and faster volume acquisition time. This system demonstrates easy instrumentation access, low photo damage for continuous observation, and high compatibility with general cell assays, providing a promising methodological pathway to investigate a wide range of intracellular processes and functions with precise spatiotemporal contextual details.
Article
Oncology
Simon F. Merz, Philipp Jansen, Ricarda Ulankiewicz, Lea Bornemann, Tobias Schimming, Klaus Griewank, Zuelal Cibir, Andreas Kraus, Ingo Stoffels, Timo Aspelmeier, Sven Brandau, Dirk Schadendorf, Eva Hadaschik, Gernot Ebel, Matthias Gunzer, Joachim Klode
Summary: This study utilized LSFM technology to comprehensively analyze SLNs of melanoma patients, revealing metastases undetectable by routine histopathological assessment and improving the accuracy of detection and localization of metastatic cancer, potentially providing additional therapeutic options for patients.
EUROPEAN JOURNAL OF CANCER
(2021)
Article
Engineering, Biomedical
Xuejia Hu, Jingjing Zheng, Qinghao Hu, Li Liang, Dongyong Yang, Yanxiang Cheng, Sen-Sen Li, Lu-Jian Chen, Yi Yang
Summary: This study presents a smart acoustic 3D cell assembly strategy that utilizes 3D printed modules and hydrogel sheets to create versatile biomimetic micro cellular patterns. The strategy allows for the construction of vascular networks and extends the capabilities of acoustic cell assembly into 3D space. It offers innovative, flexible, and precise patterning, and holds great potential for manufacturing various artificial tissue structures that replicate in vivo functions.
Article
Multidisciplinary Sciences
Yu Shi, Timothy A. Daugird, Wesley R. Legant
Summary: Light sheet microscopes reduce phototoxicity and background while improving imaging speed. The authors quantify the differences between Gaussian and lattice light sheets using simulations and experimental data, and introduce an approach to spectrally fuse sequential acquisitions of different lattice light sheet patterns.
NATURE COMMUNICATIONS
(2022)
Review
Neurosciences
Hannah C. Bennett, Yongsoo Kim
Summary: This article highlights advances in high-resolution 3D mapping methods for studying the cerebrovascular network and neurovascular unit (NVU) in the mouse brain. The use of block-face imaging and light sheet fluorescent microscopy has enabled researchers to examine the anatomical arrangement of the cerebrovascular network and gain biological insights. These imaging techniques have the potential to advance our understanding of brain disorders and overall brain health.