Article
Virology
Hsu-Feng Chu, Shu-Chun Cheng, Chiao-Yin Sun, Chi-Yuan Chou, Ta-Hsien Lin, Wei-Yi Chen
Summary: In this study, the crystal structure of porcine epidemic diarrhea virus (PEDV) papain-like protease 2 (PLP2) in complex with ubiquitin (Ub) was reported. The analysis revealed the interactions between PEDV PLP2 and the Ub substrate. Furthermore, the study identified key residues and a conformational feature of PEDV PLP2 that contribute to its function. These findings provide structural insights into the mechanistic effects of PEDV PLP2.
JOURNAL OF VIROLOGY
(2022)
Article
Immunology
Jihee Kim, Jaewon Yoon, Jung-Eun Park
Summary: The study identified the essential role of the M protein in the formation of PED VLPs. Immunization with PED VLPs induced IgG and IgA antibodies in mice, protecting against PED virus infection in Vero cells. PED VLPs immunization also induced Th2-dominant immune responses in mice.
Article
Immunology
Jingping Ren, Wei Zeng, Changsheng Jiang, Chang Li, Chengjun Zhang, Hua Cao, Wentao Li, Qigai He
Summary: Porcine epidemic diarrhea virus (PEDV) causes detrimental effects on the global swine industry. The study demonstrates that cinchonine can effectively inhibit PEDV replication by inducing cellular autophagy, suggesting its potential as a novel antiviral agent.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Haifei Wang, Peng Hui, Yoshinobu Uemoto, Yueyun Ding, Zongjun Yin, Wenbin Bao
Summary: This study investigated the metabolic and proteomic characteristics of PEDV-infected porcine intestinal epithelial cells using liquid chromatography tandem mass spectrometry and isobaric tags for relative and absolute quantification techniques. Changes in metabolism related to cysteine and methionine metabolism, glycine, serine, and threonine metabolism, and mineral absorption were observed. The betaine-homocysteine S-methyltransferase (BHMT) gene was found to potentially regulate these processes and knockdown of BHMT decreased PEDV replication and virus titers.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Virology
Alice-Roza Eruera, Alice M. McSweeney, Geena M. McKenzie-Goldsmith, Helen K. Opel-Reading, Simone X. Thomas, Ashley C. Campbell, Louise Stubbing, Andrew Siow, Jonathan G. Hubert, Margaret A. Brimble, Vernon K. Ward, Kurt L. Krause
Summary: Norovirus is a major cause of viral gastroenteritis worldwide. This study provides new insights for drug design against GII.4 genogroup of noroviruses.
Article
Veterinary Sciences
Yun Chen, Yu Zhang, Qiyuan Luo, Yongjian Zhu, Huijun Du, Suya Liao, Yuhui Yang, Huricha Chen
Summary: The study evaluated the antiviral activity of Alpiniae oxyphyllae fructus polysaccharide 3 (AOFP3) against PEDV infection, showing that AOFP3 decreased PEDV titer, prevented cellular damage, and exhibited antioxidative activity.
RESEARCH IN VETERINARY SCIENCE
(2021)
Article
Microbiology
Junyong Mao, Xiaojun Huang, Ying Shan, Jidong Xu, Qin Gao, Xiaohan Xu, Chuni Zhang, Fushan Shi, Min Yue, Fang He, Weihuan Fang, Xiaoliang Li
Summary: In this study, the gene expression patterns of host responses to PEDV infection were investigated using RNA-seq. The results showed that PEDV infection significantly affected the gene expression of the host, particularly those related to lipid metabolism. Additionally, treatment with palmitic acid reduced the replication of PEDV. Further research is needed to understand the mechanisms underlying the inhibition of PEDV replication by palmitic acid.
VETERINARY MICROBIOLOGY
(2022)
Article
Virology
Mingxia Li, Liping Zhang, Li Pan, Peng Zhou, Ruiming Yu, Zhongwang Zhang, Jianliang Lv, Huichen Guo, Yonglu Wang, Sa Xiao, Xinsheng Liu
Summary: In this study, the researchers found that nicotinamide (NAM) effectively inhibits the replication of PEDV and PDCoV by primarily targeting their RNA and protein synthesis processes. The activation of the ERK1/2/MAPK pathway and downregulation of transcription factors also contribute to the inhibition of virus replication. This suggests that NAM could be a promising antiviral candidate not only for swine intestinal coronaviruses but also for other human and animal coronaviruses.
Article
Microbiology
Xiaoting Wang, Bingqing Chen, Ruisong Yu, Fusheng Si, Chunfang Xie, Zhen Li, Shijuan Dong, Daojing Zhang
Summary: Porcine epidemic diarrhea virus (PEDV) continues to pose a threat to the swine industry worldwide. This study evaluated the effectiveness of magnolol (MAG) as a potential inhibitor of PEDV. In vitro testing showed that MAG exhibited anti-PEDV activity, suppressing viral replication. However, further in vivo testing using animal hosts is necessary to determine its efficacy.
Article
Microbiology
Yingshuo Sun, Ting Gong, Dongdong Wu, Yongzhi Feng, Qi Gao, Jiabao Xing, Xiaoyu Zheng, Zebu Song, Xing Liu, Xiongnan Chen, Yankuo Sun, Guihong Zhang, Lang Gong
Summary: In this study, two strains of PEDV were isolated from diarrheal samples collected from two new PED outbreak farms in 2022. The strains were analyzed for their gene sequences and pathogenicity. The results showed that both strains belong to the G2c subtype and can cause severe diarrhea and intestinal tissue lesions in infected piglets. It was also found that PEDV can be detected in various organs, with the highest levels in the intestinal tissues.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Zhiwei Li, Zhiqian Ma, Linfang Dong, Ting Yang, Yang Li, Dian Jiao, Weiguo Han, Haixue Zheng, Shuqi Xiao
Summary: This study confirms that the S gene is the decisive gene for PEDV adaptability to monkey Vero cells and porcine LLC-PK1 cells. The exchange of S1 and S2 subunits in adaptive strains does not enable PEDV to adapt to cells, and the 803L and 976H residues in the S2 subunit are critical for rCH/SX/2016-S1(HNXP)+S3(HNXP) adaptation to Vero cells. These findings highlight the crucial role of the PEDV S protein in cell tropism and suggest the potential role of coronaviruses S protein in cross-species transmission. Additionally, this study provides unique insights into identifying PEDV functional receptors and developing PEDV and other coronaviruses vaccines.
Article
Immunology
Mingxiang Li, Yiye Zhang, Yuxin Fang, Shaobo Xiao, Puxian Fang, Liurong Fang
Summary: Porcine epidemic diarrhea virus (PEDV) is a re-emerging enteropathogenic coronavirus. In this study, a recombinant trypsin-independent PEDV strain was developed, which can provide protection against both G1 and G2 PEDV infections and does not require trypsin for propagation.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Pharmacology & Pharmacy
Hongyu Rao, Weiwei Su, Xiaoli Zhang, Yonggang Wang, Tao Li, Jinliang Li, Xuan Zeng, Peibo Li
Summary: In this study, Hypericum japonicum extract (HJ) was found to have antiviral effects against PEDV. In vitro experiments showed that HJ could directly inactivate PEDV and inhibit its proliferation at non-cytotoxic concentrations. In vivo experiments demonstrated that HJ could reduce viral titers in the intestines of infected piglets and improve their intestinal pathological, suggesting that HJ has the potential to be developed as an anti-PEDV drug.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Microbiology
Suttipun Sungsuwan, Supasek Kadkanklai, Wuttichai Mhuantong, Anan Jongkaewwattana, Peera Jaru-Ampornpan
Summary: Coronaviruses pose threats to agriculture and human health, and understanding how they evade host defense can help manage infections. Zinc-finger antiviral protein (ZAP) has antiviral effects against coronaviruses. The virus uses its own nucleocapsid protein to interact with ZAP and counteract its activity.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Jie Ren, Zhen Ding, Puxian Fang, Shaobo Xiao, Liurong Fang
Summary: PEDV nsp13 is a crucial protein with ATPase and helicase activities that play a key role in unwinding viral RNA. By studying its biochemical properties, new insights have been provided for the development of antiviral drugs.
VETERINARY MICROBIOLOGY
(2021)