Article
Oncology
Maria Gabriela O. Fernandes, Natalia Cruz-Martins, Conceicao Souto Moura, Susana Guimaraes, Joana Pereira Reis, Ana Justino, Maria Joao Pina, Adriana Magalhaes, Henrique Queiroga, Jose Carlos Machado, Venceslau Hespanhol, Jose Luis Costa
Summary: Plasma ctDNA testing showed high accuracy and clinical relevance in newly diagnosed advanced NSCLC patients, highlighting its potential as a valuable tool for mutation detection in clinical practice.
Article
Oncology
Szilvia Lilla Csoma, Judit Bedekovics, Gergo Veres, Anita arokszallasi, Csilla Andras, Gabor Mehes, Attila Mokanszki
Summary: In the era of personalized oncology, next-generation sequencing plays a crucial role in identifying genetic aberrations in biliary tract cancers. Our study demonstrates that liquid biopsy of peripheral blood can effectively monitor the molecular genetic profile and chemotherapy response in BTCs, providing a minimally invasive testing approach for precision oncology treatment.
Article
Oncology
Sehhoon Park, Steve Olsen, Bo Mi Ku, Min-Sang Lee, Hyun-Ae Jung, Jong-Mu Sun, Se-Hoon Lee, Jin Seok Ahn, Keunchil Park, Yoon-La Choi, Myung-Ju Ahn
Summary: This study analyzed the concordance of actionable genomic alterations in non-small cell lung cancer patients detected through circulating tumor DNA (ctDNA) and tissue sequencing. The results showed that ctDNA testing can identify additional actionable genomic alterations beyond tissue testing.
Article
Oncology
Chenglong Zhao, Jianghua Li, Yongchang Zhang, Rui Han, Yubo Wang, Li Li, Yimin Zhang, Mengxiao Zhu, Jie Zheng, Haiwei Du, Chen Hu, Chengzhi Zhou, Nong Yang, Shangli Cai, Yong He
Summary: This study found that the combination of carcinoembryonic antigen (CEA) and CYFRA21-1 can effectively predict the concordance between plasma and tissue biopsies, which can be used to evaluate the priority of plasma and tissue biopsies for gene testing and guide clinical applications in lung adenocarcinoma patients.
Article
Oncology
Wanyuan Cui, Charlotte Milner-Watts, Hazel O'Sullivan, Hannah Lyons, Anna Minchom, Jaishree Bhosle, Michael Davidson, Nadia Yousaf, Sophie Scott, Iris Faull, Marina Kushnir, Rebecca Nagy, Mary O'Brien, Sanjay Popat
Summary: The study compared the clinical utility of upfront cfDNA-NGS with routine tissue testing in patients with aNSCLC, finding that cfDNA-NGS can increase the detection rate of tier-1 variants, show high concordance with tissue results, and halve time-to-treatment.
EUROPEAN JOURNAL OF CANCER
(2022)
Article
Oncology
Cloud P. Paweletz, Grace A. Heavey, Yanan Kuang, Emily Durlacher, Thian Kheoh, Richard C. Chao, Alexander I. Spira, Konstantinos Leventakos, Melissa L. Johnson, Sai-Hong Ignatius Ou, Gregory J. Riely, Kenna Anderes, Wenjing Yang, James G. Christensen, Pasi A. Janne
Summary: This study reports on early ctDNA changes of KRAS G12C in lung cancer patients and suggests that ctDNA changes can be used as a potential measure for early prediction of clinical response.
CLINICAL CANCER RESEARCH
(2023)
Article
Oncology
Chul Seung Lee, Hoon Seok Kim, Jeoffrey Schageman, In Kyu Lee, Myungshin Kim, Yonggoo Kim
Summary: Circulating tumor DNA (ctDNA) is a minimally invasive biomarker that can be analyzed using next-generation sequencing (NGS) to evaluate its clinical and analytical performance in colorectal cancer (CRC) patients. Postoperative ctDNA detection can serve as a valuable marker for identifying the risk of recurrence or persistent tumor lesions in patients with CRC.
Review
Oncology
Maria Gabriela O. Fernandes, Natalia Cruz-Martins, Jose Carlos Machado, Jose Luis Costa, Venceslau Hespanhol
Summary: Liquid biopsy, specifically plasma ctDNA analysis, has revolutionized cancer patient management by detecting genomic alterations and resistance mechanisms, particularly in lung cancer patients. While offering a comprehensive view of tumor diversity, its non-invasive nature overcomes the challenges of obtaining tissue samples. Further clarification is needed regarding the use of LB in supporting diagnostic and therapeutic decisions.
CANCER CELL INTERNATIONAL
(2021)
Article
Oncology
Zeyun Lin, Yuqin Li, Shiqi Tang, Qiuhua Deng, Juhong Jiang, Chengzhi Zhou
Summary: This study compared the molecular profiles of tissue and plasma samples, and found that tissue-NGS has a higher sensitivity in detecting genetic alterations compared to plasma-NGS. Tissue-NGS remains the preferred method for evaluating the molecular profile of NSCLC patients when tumor tissue is available, but plasma can be used as substitute material when tissue is unavailable.
Article
Biochemistry & Molecular Biology
Woo Kyung Ryu, Sekyung Oh, Jun Hyeok Lim, Seung Jae Lee, Hyun-Tae Shin, Jeong-Seon Ryu
Summary: Recent research suggests that changes in ctDNA levels are correlated with TB changes, and the detection of ctDNA can be used to predict new metastases, rapid tumor growth, and poor survival in NSCLC patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Panagiota Economopoulou, Aris Spathis, Ioannis Kotsantis, Eirini Maratou, Maria Anastasiou, Myrto K. Moutafi, Maria Kirkasiadou, Anastasios Pantazopoulos, Maria Giannakakou, Daniel L. Edelstein, Hillary Sloane, Johannes Fredebohm, Frederick S. Jones, Anastasios Kyriazoglou, Niki Gavrielatou, Periklis Foukas, Ioannis Panayiotides, Amanda Psyrri
Summary: The aim of this pilot study was to evaluate somatic mutations in tumor and circulating DNA samples from HNSCC patients and assess the association of changes in ctDNA levels with survival. The study included 62 HNSCC patients treated with surgery or radical chemoradiotherapy. Tumor DNA was extracted from plasma and tumor tissue, and the presence of pathogenic variants in four genes was assessed.
Article
Oncology
Masato Kojima, Takanori Harada, Takahiro Fukazawa, Sho Kurihara, Ryo Touge, Isamu Saeki, Shinya Takahashi, Eiso Hiyama
Summary: In this study, single-cell next-generation sequencing of circulating tumor cells (CTCs) from neuroblastoma (NB) patients was performed. The findings showed that the isolation rate of CTCs was associated with disease progression, and single-cell RNA sequencing revealed upregulated genes related to angiogenesis and cell cycle in CTCs. These results indicate the utility of single-cell CTC sequencing in characterizing NB tumor biology and metastasis mechanisms.
Article
Respiratory System
Ya-Sian Chang, Siang-Jyun Tu, Yu-Chia Chen, Ting-Yuan Liu, Ya-Ting Lee, Ju-Chen Yen, Hsin-Yuan Fang, Jan-Gowth Chang
Summary: This study identified 19 known driver mutations and new variants in Taiwanese patients with non-small cell lung cancer. EGFR mutations were found at a higher frequency compared to Caucasians, while KRAS and TP53 mutations were less common. All ClinVar pathogenic variants were trunk mutations present in the tumor regions.
RESPIRATORY RESEARCH
(2021)
Article
Oncology
Russell J. Diefenbach, Jenny H. Lee, Ashleigh Stewart, Alexander M. Menzies, Matteo S. Carlino, Robyn P. M. Saw, Jonathan R. Stretch, Georgina V. Long, Richard A. Scolyer, Helen Rizos
Summary: This study developed a custom panel for detecting TERT promoter mutations in ctDNA from melanoma patients. Analysis of patient samples showed a high detection rate for BRAF, NRAS, and TERT promoter mutations, and the panel demonstrated consistency with tissue biopsies.
FRONTIERS IN ONCOLOGY
(2022)
Article
Oncology
Martin Zacharias, Gudrun Absenger, Karl Kashofer, Robert Wurne, Joerg Lindenmann, Angelika Terbuch, Selma Konjic, Stefan Sauer, Franz Gollowitsch, Gregor Gorkiewicz, Luka Brcic
Summary: The study demonstrated that incorporating RNA-based panels in the reflex testing approach leads to more detected fusions and increased targeted therapies for lung cancer patients. This comprehensive molecular profiling strategy helps identify patients with emerging biomarkers in the rapidly evolving landscape of targeted therapies.
TRANSLATIONAL LUNG CANCER RESEARCH
(2021)
Article
Oncology
Julia K. Rotow, Philippe Gui, Wei Wu, Victoria M. Raymond, Richard B. Lanman, Frederic J. Kaye, Nir Peled, Ferran Fece de la Cruz, Brandon Nadres, Ryan B. Corcoran, Iwei Yeh, Boris C. Bastian, Petr Starostik, Kimberly Newsom, Victor R. Olivas, Alexander M. Wolff, James S. Fraser, Eric A. Collisson, Caroline E. McCoach, D. Ross Camidge, Jose Pacheco, Lyudmila Bazhenova, Tianhong Li, Trever G. Bivona, Collin M. Blakely
CLINICAL CANCER RESEARCH
(2020)
Article
Oncology
Lindsey M. Charo, Ramez N. Eskander, Ryosuke Okamura, Sandip P. Patel, Mina Nikanjam, Richard B. Lanman, David E. Piccioni, Shumei Kato, Michael T. McHale, Razelle Kurzrock
Summary: The molecular characterization of gynecologic cancers through ctDNA analysis plays a vital role in determining prognosis and guiding personalized therapy. This study found that higher ctDNA mutation frequency is associated with poor overall survival, while therapy tailored to ctDNA alterations leads to improved outcomes. The concordance between tissue and ctDNA genomic results remains high, demonstrating the potential utility of ctDNA in individualizing cancer therapy for gynecologic cancer patients.
MOLECULAR ONCOLOGY
(2021)
Article
Oncology
Scott Kopetz, Katherine A. Guthrie, Van K. Morris, Heinz-Josef Lenz, Anthony M. Magliocco, Dipen Maru, Yibing Yan, Richard Lanman, Ganiraju Manyam, David S. Hong, Alexey Sorokin, Chloe E. Atreya, Luis A. Diaz, Carmen Allegra, Kanwal P. Raghav, Stephen E. Wang, Christopher H. Lieu, Shannon L. McDonough, Philip A. Philip, Howard S. Hochster
Summary: Combination therapy of vemurafenib and irinotecan in patients with BRAF(V600E)-mutated colorectal cancer has shown improved progression-free survival, response rate, and disease control rate, as well as reduced BRAF(V600E) variant allele frequency in circulating tumor DNA.
JOURNAL OF CLINICAL ONCOLOGY
(2021)
Article
Oncology
Vincent K. Lam, Jianjun Zhang, Carol C. Wu, Hai T. Tran, Lerong Li, Lixia Diao, Jing Wang, Waree Rinsurongkawong, Victoria M. Raymond, Richard B. Lanman, Jeff Lewis, Emily B. Roarty, Jack Roth, Stephen Swisher, J. Jack Lee, Don L. Gibbons, Vassiliki A. Papadimitrakopoulou, John Heymach
Summary: The study found a moderate but significant correlation between ctDNA variant allele frequency and imaging measures of tumor burden, with varying strengths based on tumor genotypes. Factors such as TP53 and EGFR mutations, visceral metastasis, and tumor burden were independent predictors of increased ctDNA shedding, suggesting that ctDNA levels are affected by both tumor burden and genotype.
JOURNAL OF THORACIC ONCOLOGY
(2021)
Article
Multidisciplinary Sciences
Richard B. Lanman, Linda Hylkema, Cristie M. Boone, Brian Allee, Roger O. Castillo, Stephanie A. Moreno, Mary Faith Flores, Upuli DeSilva, Brittany Bingham, Brian M. Kemp
Summary: Understanding the historic range of a species is crucial for contemporary management and habitat restoration. Analysis of ancient fish specimens confirmed the native presence of Chinook salmon in the Guadalupe River in San Jose, California, expanding their documented historic range.
Article
Multidisciplinary Sciences
Belinda Kingston, Rosalind J. Cutts, Hannah Bye, Matthew Beaney, Giselle Walsh-Crestani, Sarah Hrebien, Claire Swift, Lucy S. Kilburn, Sarah Kernaghan, Laura Moretti, Katie Wilkinson, Andrew M. Wardley, Iain R. Macpherson, Richard D. Baird, Rebecca Roylance, Jorge S. Reis-Filho, Michael Hubank, Iris Faull, Kimberly C. Banks, Richard B. Lanman, Isaac Garcia-Murillas, Judith M. Bliss, Alistair Ring, Nicholas C. Turner
Summary: Genomic profiling of advanced breast cancer using plasma circulating tumour DNA sequencing revealed diverse subclonal resistance mutations, with distinct mutational processes identified in ER-positive breast cancer. This study highlights the importance of subclonal diversification in pre-treated advanced breast cancer, presenting novel therapeutic opportunities.
NATURE COMMUNICATIONS
(2021)
Meeting Abstract
Oncology
Ray D. Page, Leylah Drusbosky, Hiba I. Dada, Victoria M. Raymond, Davey B. Daniel, Stephen G. Divers, Karen L. Reckamp, Miguel Angel Villalona-Calero, Justin Iver Odegaard, Richard B. Lanman, Vassiliki Papadimitrakopoulou, Natasha B. Leighl
JOURNAL OF CLINICAL ONCOLOGY
(2021)
Correction
Multidisciplinary Sciences
Belinda Kingston, Rosalind J. Cutts, Hannah Bye, Matthew Beaney, Giselle Walsh-Crestani, Sarah Hrebien, Claire Swift, Lucy S. Kilburn, Sarah Kernaghan, Laura Moretti, Katie Wilkinson, Andrew M. Wardley, Iain R. Macpherson, Richard D. Baird, Rebecca Roylance, Jorge S. Reis-Filho, Michael Hubank, Iris Faull, Kimberly C. Banks, Richard B. Lanman, Isaac Garcia-Murillas, Judith M. Bliss, Alistair Ring, Nicholas C. Turner
NATURE COMMUNICATIONS
(2021)
Article
Oncology
Ray D. Page, Leylah M. Drusbosky, Hiba Dada, Victoria M. Raymond, Davey B. Daniel, Stephen G. Divers, Karen L. Reckamp, Miguel A. Villalona-Calero, Daniel Dix, Justin Odegaard, Richard B. Lanman, Vassiliki A. Papadimitrakopoulou, Natasha B. Leighl
Summary: Liquid biopsy provides a fast and noninvasive method for genomic profiling in patients with newly diagnosed, advanced NSCLC, allowing the identification of NCCN-recommended biomarkers and informing first-line therapy options. This study demonstrates that liquid biopsy achieves similar treatment outcomes compared to tissue-genotyping, while offering a significantly faster initiation of targeted therapy. Liquid biopsy can be a valuable tool in guiding targeted therapy decisions at the time of diagnosis in advanced disease.
CLINICAL LUNG CANCER
(2022)
Article
Oncology
James T. Topham, Chris J. O'Callaghan, Harriet Feilotter, Hagen F. Kennecke, Young S. Lee, Weimin Li, Kimberly C. Banks, Katie Quinn, Daniel J. Renouf, Derek J. Jonker, Dongsheng Tu, Eric X. Chen, Jonathan M. Loree
Summary: This study aimed to explore the acquired resistance mechanisms in metastatic colorectal cancer patients after anti-epidermal growth factor receptor (EGFR) antibody treatment. The results indicated that multiple genes experienced increased mutation frequency, concurrent alterations, and copy gains in patients following anti-EGFR therapy. Furthermore, gene fusions were found to be associated with prior anti-EGFR therapy. The study also highlighted the prevalence of polyclonal resistance in patients with prior anti-EGFR treatment. This research provides valuable insights into the acquired resistance mechanisms of anti-EGFR therapy.
JOURNAL OF CLINICAL ONCOLOGY
(2023)
Article
Oncology
Morten Lapin, Helen J. Huang, Sharmeen Chagani, Milind Javle, Rachna T. Shroff, Shubham Pant, Mohamed A. Gouda, Anjali Raina, Kiran Madwani, Veronica R. Holley, S. Greg Call, Derek J. Dustin, Richard B. Lanman, Funda Meric-Bernstam, Victoria M. Raymond, Lawrence N. Kwong, Filip Janku
Summary: In patients with IDH-mutated advanced cholangiocarcinoma, detection of IDH mutations in ctDNA is feasible and dynamic changes in ctDNA may correspond with the clinical course and clonal evolution. High detection rates were observed using ddPCR and NGS for analyzing ctDNA samples.
JCO PRECISION ONCOLOGY
(2022)
Article
Oncology
Ramon Palmero, Alvaro Taus, Santiago Viteri, Margarita Majem, Enric Carcereny, Javier Garde-Noguera, Enriqueta Felip, Ernest Nadal, Andrea Malfettone, Miguel Sampayo, Francois Riva, Rebecca J. Nagy, Richard B. Lanman, Iris Faull, Daniel Dix, Niki Karachaliou, Rafael Rosell
Summary: This study found that comprehensive cfDNA testing is as effective as tissue testing in detecting guideline-recommended biomarkers in aNSCLC. First-line therapy based on cfDNA produced similar outcomes to tissue-based testing, highlighting the clinical utility of comprehensive cfDNA genotyping as an initial modality in treatment-naive aNSCLC patients.
JCO PRECISION ONCOLOGY
(2021)
Meeting Abstract
Oncology
Javier Pascual, Rosalind J. Cutts, Belinda Kingston, Sarah Hrebien, Lucy S. Kilburn, Sarah Kernaghan, Laura Moretti, Katie Wilkinson, Andrew M. Wardley, Iain R. Macpherson, Richard D. Baird, Rebecca Roylance, Michael Hubank, Giselle Walsh, Iris Faull, Kimberly C. Banks, Richard B. Lanman, Isaac Garcia-Murillas, Judith M. Bliss, Alistair Ring, Nicholas C. Turner
Article
Fisheries
Leigh Marymor, Richard Burnham Lanman
Summary: Recent evidence suggests that North American beavers were historically native to California's coast, but symbols representing beavers are poorly represented in California rock art, especially in the San Francisco Bay Area. A novel record of Western Message Petroglyphs indicates the presence of a beaver lodge in the late nineteenth century in the Alameda Creek watershed, potentially the last evidence of beavers in the region before their extinction due to the fur trade.
CALIFORNIA FISH AND WILDLIFE JOURNAL
(2021)
Meeting Abstract
Oncology
Jason Agulnik, Jennifer H. Law, Rosalyn Juergens, Janessa Laskin, Scott Laurie, Desiree Hao, Doreen A. Ezeife, Lisa W. Le, Lesli A. Kiedrowski, Richard B. Lanman, Natasha B. Leighl
CLINICAL CANCER RESEARCH
(2020)