Article
Biochemistry & Molecular Biology
Dhani Ram Mahato, Magnus Andersson
Summary: The function of Na+,K+-ATPases in biological membranes depends on the surrounding lipid environment. Lipid-interaction sites A and B have been confirmed to activate and stabilize the protein, while the location of inhibitory site C has yet to be experimentally determined. Disease mutants in the membrane-protein interface can also affect lipid interactions. In this study, simulations were used to determine the lipid-protein interactions of wild-type and disease mutant Na+,K+-ATPases, revealing that lipid interactions are highly state-dependent and can be disrupted by disease mutations in the lipid interface.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2023)
Article
Oncology
Shinya Matsumura, Takeshi Ishikawa, Juichiro Yoshida, Ryuichi Morita, Tomoki Sakakida, Yuki Endo, Toshifumi Doi, Ryohei Hirose, Ken Inoue, Osamu Dohi, Naohisa Yoshida, Kazuhiko Uchiyama, Tomohisa Takagi, Hideyuki Konishi, Kohichiroh Yasui, Yuji Naito, Yoshito Itoh
Summary: This study evaluated the effect of proton pump inhibitors (PPIs) on chemotherapy for esophageal cancer. The results showed that PPIs can enhance the sensitivity of esophageal cancer cells to 5-FU and improve overall survival in patients undergoing 5-FU-based therapy.
Article
Biochemistry & Molecular Biology
Jingshi Liu, Yongdong Niu, Bin Zhang, Qisi Sun, Haiyi Li, Lu Bai, Zhongjing Su
Summary: The expression levels of GPER1 are found to be associated with tumor type and survival rates in esophageal carcinoma. In overall esophageal carcinoma, low expression of GPER1 is correlated with poor survival rates, while overexpression of GPER1 is associated with the development of esophageal adenocarcinoma and its pre-cancerous lesion. Furthermore, the protein expression of GPER1 is higher in esophageal adenocarcinoma tissues compared to normal esophageal tissues, whereas it is lower in esophageal squamous cell carcinoma tissues.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Clinical Neurology
Samira S. Valvassori, Jefte Peper-Nascimento, Jorge M. Aguiar-Geraldo, Amanda Hilsendeger, Thiani Daminelli, Mario F. Juruena, Rif S. El-Mallakh, Joao Quevedo
Summary: This study found significant differences in biological rhythms, Na+, K+-ATPase, and oxidative stress parameters between bipolar disorder patients and healthy controls, highlighting the importance of these parameters in relation to BD.
JOURNAL OF AFFECTIVE DISORDERS
(2023)
Article
Oncology
Huilai Lv, Yang Tian, Jiachen Li, Chao Huang, Bokang Sun, Chunyue Gai, Zhenhua Li, Ziqiang Tian
Summary: This study evaluated the efficacy and safety of neoadjuvant sintilimab plus chemotherapy in patients with resectable locally advanced esophageal squamous cell carcinoma (ESCC). The results showed that this treatment approach achieved a good pathological complete response rate and had good tolerability.
FRONTIERS IN ONCOLOGY
(2022)
Article
Oncology
Fazlur Rahman Talukdar, Sheila C. Soares Lima, Rita Khoueiry, Ruhina Shirin Laskar, Cyrille Cuenin, Bruna Pereira Sorroche, Anne-Claire Boisson, Behnoush Abedi-Ardekani, Christine Carreira, Diana Menya, Charles P. Dzamalala, Mathewos Assefa, Abraham Aseffa, Vera Miranda-Goncalves, Carmen Jeronimo, Rui M. Henrique, Ramin Shakeri, Reza Malekzadeh, Nagla Gasmelseed, Mona Ellaithi, Nitin Gangane, Daniel R. S. Middleton, Florence Le Calvez-Kelm, Akram Ghantous, Maria Leon Roux, Joachim Schuz, Valerie McCormack, M. Iqbal Parker, Luis Felipe Ribeiro Pinto, Zdenko Herceg
Summary: This study investigated tumor-specific DNA methylation in ESCC cases from high-incidence countries, identifying thousands of differentially methylated positions and regions, particularly in gene regions associated with transcription activation. Prioritized genes for replication showed consistent methylation differences and downregulation in tumors. The study highlights the potential of these DNA methylation events as tumor-specific markers for further biomarker discovery.
Article
Environmental Sciences
Priya Gupta, Archisman Mahapatra, Anjali Suman, Rahul Kumar Singh
Summary: This study investigated the effects of cypermethrin, a synthetic pesticide, on the development of zebrafish embryos. The results showed that cypermethrin exposure led to abnormal development, decreased heart rate, and disruption of cardiac development and ion regulation. Computational docking also revealed interactions between cypermethrin and developmental proteins. This study provides important information on the ecotoxicological risk and the mode of action of cypermethrin.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Genetics & Heredity
Yi Shen, Yi Shao, Chen Niu, Xiaoli Ruan, Zhaoping Zang, Rena Nakyeyune, Xiuhua Guo, Fen Liu
Summary: This study identified 33 differentially expressed circRNAs in the blood of ESCC patients, and constructed an ESCC-associated circRNA-miRNA-mRNA network. Enrichment analyses showed the association of DEGs with various biological processes and cancer-related pathways.
FRONTIERS IN GENETICS
(2021)
Article
Biochemistry & Molecular Biology
Yongqing Yang, Xiuli Han, Liang Ma, Yujiao Wu, Xiao Liu, Haiqi Fu, Guoyong Liu, Xiaoguang Lei, Yan Guo
Summary: Plant metabolites are dynamically modified and distributed in response to environmental changes. Maintaining ion homeostasis under salt stress requires coordinated activation of two types of central regulators: plasma membrane (PM) H+-ATPase and Na+/H+ antiporter. In this study, we identified phosphatidylinositol (PI) and phosphatidylinositol 4-phosphate (PI4P) as key molecules in regulating ion homeostasis under salt stress in Arabidopsis.
Article
Biology
Elias Rizk, Andy Madrid, Joyce Koueik, Dandan Sun, Krista Stewart, David Chen, Susan Luo, Felissa Hong, Ligia A. Papale, Nithya Hariharan, Reid S. Alisch, Bermans J. Iskandar
Summary: FACS-based sorting was used to investigate the regulation of axon regeneration in retinal ganglion cells (RGCs) following optic nerve transection in rats. The study revealed the involvement of Na+/K+-ATPase and DNA methylation in axon regeneration, with specific genes and pathways identified. Inhibition of Na+/K+-ATPase led to reduced RGC axon regeneration, highlighting its importance as a key regulator of CNS axon regeneration. Reactivation of embryonic signals was also found to be necessary for successful regeneration. These findings provide critical insights into the molecular mechanisms of axon regeneration in the mammalian CNS.
COMMUNICATIONS BIOLOGY
(2023)
Article
Oncology
Rong Li, Xing-Feng Pang, Zhi-Guang Huang, Li-Hua Yang, Zhi-Gang Peng, Jie Ma, Rong-Quan He
Summary: This study revealed that UBE2C mRNA and protein levels were highly expressed in ESCC and may play different roles in different stages of the disease. UBE2C mainly influences the biological function of esophageal cancer through synergistic effects with genes such as CDK1, PTTG1, and SKP2. Additionally, a potential UBE2C-related ceRNA network (HCP5/has-miR-139-5p/UBE2C) was constructed for ESCC.
Article
Genetics & Heredity
Shang-Wei Chen, Hua-Fu Zhou, Han-Jie Zhang, Rong-Quan He, Zhi-Guang Huang, Yi-Wu Dang, Xia Yang, Jun Liu, Zong-Wang Fu, Jun-Xian Mo, Zhong-Qing Tang, Chang-Bo Li, Rong Li, Li-Hua Yang, Jie Ma, Lin-Jie Yang, Gang Chen
Summary: Esophageal squamous cell carcinoma (ESCC) is the major histological type of esophageal cancers. The transcription factor PTTG1 was found to be highly expressed in ESCC tissues and may interact with target genes through chemokines and cytokine signaling pathways, particularly SLC25A17 and ERH. This suggests that PTTG1 may play a crucial role in the formation of ESCC by regulating downstream target genes.
FRONTIERS IN GENETICS
(2021)
Article
Obstetrics & Gynecology
T. Hirakawa, M. Goto, K. Takahashi, T. Iwasawa, A. Fujishima, K. Makino, H. Shirasawa, W. Sato, T. Sato, Y. Kumazawa, Y. Terada
Summary: The study investigated the expression and localization of specific Na+/K+ ATPase isoforms in human blastocysts and their association with embryonic developmental behavior. The results showed that Na+/K+ ATPase alpha 1, beta 1, and beta 3 isoforms were expressed in human blastocysts, but their expression levels were not correlated with blastocyst expansion rate. The alpha 1 and beta 3 isoforms were localized to the basolateral membrane of trophectoderm cells, while the beta 3 isoform was localized between trophectoderm cells.
HUMAN REPRODUCTION
(2022)
Article
Oncology
Wencheng Zhang, Cihui Yan, Xuan Gao, Xiaoxia Li, Fuliang Cao, Gang Zhao, Jingjing Zhao, Puchun Er, Tian Zhang, Xi Chen, Yuwen Wang, Yao Jiang, Quanren Wang, Baozhong Zhang, Dong Qian, Jun Wang, Dejun Zhou, Xiubao Ren, Zhentao Yu, Lujun Zhao, Zhiyong Yuan, Ping Wang, Qingsong Pang
Summary: The study showed that radiotherapy plus anti-PD-1 antibody as first-line therapy for locally advanced esophageal squamous cell carcinoma is safe and feasible, with tumor-infiltrating and peripheral lymphocytes being associated with patient survival, suggesting potential for further exploration as a treatment option.
Article
Genetics & Heredity
Xijuan Yao, Hui Chen, Bing Xu, Jing Lu, Junjie Gu, Fangyu Chen, Mengyang Ju, Xinchen Sun
Summary: This study aimed to investigate the role of ATP6V1C1 in radiotherapy resistance in esophageal squamous cell carcinoma (ESCC) cells. The results showed that suppressing ATP6V1C1 increased the sensitivity of ESCC cells to radiotherapy, while also inhibiting autophagy by activating the mTOR signaling pathway to suppress radiosensitivity. These findings suggest that ATP6V1C1 may serve as a potential therapeutic target to enhance radiation sensitivity in ESCC.
Article
Environmental Sciences
Sun-Wung Hsieh, Szu-Chia Chen, Chun-Hung Chen, Ming-Tsang Wu, Chih-Hsing Hung
Summary: This study aims to investigate the risks of cognition from incense smoke. Data from Taiwan Biobank in the community were obtained. The study found that incense exposure is one of the independent risk factors for cognitive decline.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL HEALTH RESEARCH
(2023)
Article
Public, Environmental & Occupational Health
Yun-Shiuan Chuang, Chun-Ying Lee, Pei-Chen Lin, Chih-Hong Pan, Hui-Min Hsieh, Chia-Fang Wu, Ming-Tsang Wu
Summary: This study found possible correlations between occupational exposure to lead, chlorinated solvents, benzene, and asbestos and breast cancer risk among female workers, suggesting a need for regular screening for breast cancer for employees exposed to these special workplace hazards.
INTERNATIONAL ARCHIVES OF OCCUPATIONAL AND ENVIRONMENTAL HEALTH
(2022)
Article
Biochemistry & Molecular Biology
Peter G. Osborne, Raghavendra Rao Pasupuleti, Ming-Tsang Wu, Chien-Hung Lee, Vinoth Kumar Ponnusamy
Summary: Betel quid (BQ), an addictive carcinogenic stimulant chewed by millions in Asia, contains slaked lime which affects the levels of alkaloids in the betel quid. Chewing BQ leads to stimulant effects, while unripe green betel nut could potentially be used as a replacement therapy for BQ substance use disorder.
PROCESS BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Wei-Chung Chen, Chun-Chieh Wu, Yi-Hsun Chen, Jui-Ying Lee, Yao-Kuang Wang, Nian-Siou Wu, Ming-Tsang Wu, I-Chen Wu
Summary: This study aimed to investigate the expression of PD-L1 in esophageal squamous cell carcinoma (ESCC) tissues before and after concurrent chemoradiation therapy (CCRT). The results showed a decrease in PD-L1 expression after CCRT, and high pre-CCRT PD-L1 expression was associated with poor prognosis.
Article
Chemistry, Analytical
Yu-Ping Hsiao, Arvind Mukundan, Wei-Chung Chen, Ming-Tsang Wu, Shang-Chin Hsieh, Hsiang-Chen Wang
Summary: In this study, a biochip consisting of a light-absorbing layer of a silicon solar element and serrated interdigitated electrodes was fabricated. The biochip successfully identified four different types of cancer cells. The use of photo-excited carriers and the concentration catalysis mechanism of GSH/GSSG enabled fast and label-free measurement of cancer cells. The analysis time was short, and the results were accurate.
Article
Environmental Sciences
Yu-Ming Hsu, Chia-Fang Wu, Min-Zong Huang, Jentaie Shiea, Chih-Hung Pan, Chia-Chu Liu, Chu-Chih Chen, Yin-Han Wang, Ching-Mei Cheng, Ming-Tsang Wu
Summary: This study utilized a rapid TD-ESI/MS screening technique to assess melamine exposure in workers and created avatar-like body images to study temporal and dynamic changes in exposure. The results showed variations in melamine exposure among different worksites and a linear relationship between body image scores and urinary melamine levels.
Article
Environmental Sciences
Sabelo Bonginkosi Dlamini, Ming-Tsang Wu, Hans-Uwe Dahms
Summary: This study investigated the association between hypertension and ART toxicity markers, and found that patients with hypertension had decreased eGFR. The study also showed a significant association between time of the study and eGFR. These findings highlight the need for special attention to renal impairment in PLHIV with hypertension during ART treatment.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Environmental Sciences
Tsung-Lin Tsai, Chia-Jung Hsieh, Ming-Tsang Wu, Mei-Lien Chen, Po-Hsiu Kuo, Shu-Li Wang
Summary: Our study found that prenatal exposure to toxic metals and PAEs may contribute to certain DSM-5-oriented problems in children at 4 years of age. Reducing exposure to toxic metals and PAEs during pregnancy is recommended to prevent increased mental health problems in children.
ENVIRONMENT INTERNATIONAL
(2023)
Article
Environmental Sciences
Hsing-Kang Chen, Shu-Li Wang, Yu-Hsun Chang, Chien-Wen Sun, Ming-Tsang Wu, Mei-Lien Chen, Yu-Jie Lin, Chia -Jung Hsieh
Summary: This study examines the associations between prenatal phthalate exposure and neonatal neurobehavioral development. It found that phthalate exposure was negatively associated with neurobehaviors in female infants and positively associated with neurobehaviors in male infants. These findings highlight the gender-specific effects of maternal phthalate exposure on the neurodevelopment of newborns.
ENVIRONMENTAL POLLUTION
(2023)
Article
Biochemistry & Molecular Biology
Karthikeyan Prakasham, Swapnil Gurrani, Jen-Taie Shiea, Ming-Tsang Wu, Chia-Fang Wu, Yi-Jia Ku, Tseng-Yu Tsai, Hung-Ta Hua, Yu-Jia Lin, Po-Chin Huang, Gangadhar Andaluri, Vinoth Kumar Ponnusamy
Summary: A fast mycotoxin extraction (FaMEx) technique was developed for the rapid identification and quantification of carcinogenic ochratoxin-A (OTA) in food and agricultural soil samples. The method showed excellent linearity, sensitive detection, and low limits of quantification, making it suitable for mycotoxin detection in various samples.
Article
Medical Laboratory Technology
Hui-Ming Chen, Chou-Cheng Chen, Jia-Jen Chen, Chia-Fang Wu, Sih-Syuan Lee, Fu-Chen Kuo, Chien-Wen Sun, Mei-Lien Chen, Chia-Jung Hsieh, Shu-Li Wang, Ming-Tsang Wu
Summary: This study aims to establish reference intervals for important biochemical parameters in cord blood of newborns in Taiwan and investigate their sex differences. It also examines the correlation between maternal blood levels and neonatal cord blood levels.
CLINICA CHIMICA ACTA
(2023)
Article
Food Science & Technology
Karthikeyan Prakasham, Swapnil Gurrani, Jentaie Shiea, Ming-Tsang Wu, Reeta Rani Singhania, Anil Kumar Patel, Cheng-Di Dong, Yu-Chia Lin, Bongee Tsai, Po-Chin Huang, Gangadhar Andaluri, Vinoth Kumar Ponnusamy
Summary: This study presents a novel semi-automated in-syringe-based fast mycotoxin extraction (IS-FaMEx) technique coupled with direct-injection electrospray-ionization tandem mass spectrometer (ESI-MS/MS) detection for the quantification of ochratoxin A in coffee and tea samples. The developed method showed remarkable linearity, with a correlation coefficient of > 0.999 and > 92% extraction recovery with a precision of 6%. The detection and quantification limits for ochratoxin A were lower than the European Union regulatory limit, making it a potential methodology for mycotoxin detection in food products.
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
(2023)
Article
Environmental Sciences
Yi-Chun Tsai, Chia-Fang Wu, Wei-Wen Hung, Ping-Shaou Yu, Chia-Chu Liu, Tusty-Jiuan Hsieh, Chu-Chih Chen, Sih-Syuan Li, Jia-Jen Chen, Yi-Wen Chiu, Shang-Jyh Hwang, Ming-Tsang Wu
Summary: This prospective cohort study investigated the impact of melamine exposure on kidney outcomes in type 2 diabetes mellitus (T2D) patients. The findings showed a significant association between melamine exposure and adverse kidney outcomes in T2D patients, especially in those with male, well sugar control or good baseline kidney function.
ENVIRONMENTAL POLLUTION
(2023)
Article
Chemistry, Applied
Karthikeyan Prakasham, Swapnil Gurrani, Jentaie Shiea, Ming-Tsang Wu, Chia-Fang Wu, Yu -Chia Lin, Bongee Tsai, Po-Chin Huang, Gangadhar Andaluri, Vinoth Kumar Ponnusamy
Summary: In this study, a novel semi-automated in-syringe-based coagulant-assisted liquid-liquid microextraction (IS-CGA-LLME) technique coupled with UHPLC-MS/MS was developed for the quantification of mycotoxin (OT-A) in coffee and tea samples. The method showed highly sensitive detection and quantification limits for OT-A, excellent extraction recovery and precision. This method provides a simple, highly sensitive, semi-automated, low-matrix effect, and efficient procedure for mycotoxin determination in food samples.
Meeting Abstract
Urology & Nephrology
C-C Liu, T-J Hsieh, C-F Wu, C-H Lee, Y-C Tsai, T-Y Huang, S-C Wen, C-H Lee, T-M Chien, Y-C Lee, Y-H Chou, S-P Huang, Y-S Juan, W-J Wu, M-T Wu
