Article
Oncology
Miao He, Yingying He, Jian Xu, Youcai Zhang, Xiaoyu Cao, Li Wang, Feng Luo
Summary: This study revealed the association between FADD expression and prognosis, immune exhaustion, and tumor malignancy in LUAD patients. In addition, FADD can serve as an efficient indicator for assessing sensitivity to chemotherapy and immunotherapy, making it a potential new target for precision medicine and targeted therapy for LUAD.
FRONTIERS IN ONCOLOGY
(2023)
Article
Immunology
Kiarash Saleki, Moein Shirzad, Mostafa Javanian, Sheyda Mohammadkhani, Mohammad Hossein Alijani, Niloufarsadat Miri, Morteza Oladnabi, Abbas Azadmehr
Summary: This study aimed to investigate the role of Fas/FasL in lung involvement and mortality of COVID-19 patients. The results showed increased mFasL expression in moderate and severe COVID-19 patients, and mFas expression was inversely correlated with myalgia symptoms. Moreover, elevated sFasL protein in serum was associated with reduced lung injury and mortality, and blood profile alterations in COVID-19 patients could affect Fas/FasL complex interactions. The findings suggest that sFasL may serve as a prognostic marker for the clinical management of COVID-19 patients.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Katarzyna Gebarowska, Anna Mroczek, Jerzy R. Kowalczyk, Monika Lejman
Summary: T cell acute lymphoblastic leukemia (T-ALL) is a biologically and genetically heterogeneous disease with poor prognosis. Although miRNAs may play a vital role in T-ALL, to date, no miRNA has become a significant prognostic and diagnostic factor.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Fengling Shao, Huajie Mao, Tengling Luo, Qijun Li, Lei Xu, Yajun Xie
Summary: Abnormal lipid metabolism pathways in LUAD were identified by analyzing differentially expressed genes. A novel prognostic risk score was successfully constructed and validated in LUAD samples. Knockdown of HPGDS promoted lipid synthesis and cell migration in LUAD.
FRONTIERS IN ONCOLOGY
(2022)
Article
Multidisciplinary Sciences
Hilmar Quentmeier, Robert Geffers, Vivien Hauer, Stefan Nagel, Claudia Pommerenke, Cord C. Uphoff, Margarete Zaborski, Hans G. Drexler
Summary: Overexpression of antiapoptotic BCL2 family proteins occurs in various hematologic malignancies and contributes to tumorigenesis by inhibiting the apoptotic machinery of the cells. The BH3 mimetics provide an option for medication, with venetoclax being the first drug applied for chronic lymphocytic leukemia and acute myeloid leukemia. The expression screening of cell lines using the LL-100 panel revealed that anaplastic large cell lymphoma (ALCL) and primary effusion lymphoma (PEL) showed high expression of MCL1 and low expression of BCL2. The MCL1 inhibitor AZD-5991 induced apoptosis in cell lines from both malignancies, suggesting its potential as a drug for these diseases. ALCL cell lines also expressed BCLXL and BCL2A1, contributing to cell survival. The combination of specific BH3 mimetics yielded synergistic effects, providing a novel strategy for the treatment of ALCL. The PI3K/mTOR inhibitor BEZ-235 could also be used in combination with AZD-5991, offering an alternative to avoid thrombocytopenia associated with BCLXL inhibitors.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Sahezeel Awadia, Merna Sitto, Sundaresh Ram, Wenbin Ji, Yajing Liu, Raheema Damani, Dipankar Ray, Theodore S. Lawrence, Craig J. Galban, Steven D. Cappell, Alnawaz Rehemtulla
Summary: FADD functions as an inhibitor of APC/C-Cdh1, leading to G1 arrest in cells. Phosphorylation and nuclear translocation of FADD are essential for its role in the cell cycle.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Elham F. Ahanin, Rebecca A. Sager, Sarah J. Backe, Diana M. Dunn, Natela Dushukyan, Adam R. Blanden, Nilamber A. Mate, Tamie Suzuki, Tyler Anderson, Meri Roy, Jasmeen Oberoi, Chrisostomos Prodromou, Imad Nsouli, Michael Daneshvar, Gennady Bratslavsky, Mark R. Woodford, Dimitra Bourboulia, John D. Chisholm, Mehdi Mollapour
Summary: In this study, a selective inhibitor of PP5, P053, was developed using an in silico approach. P053 binds to PP5 and induces apoptosis in renal cancer cells. Further investigation revealed that PP5 interacts with components of the extrinsic apoptotic pathway complex II, suggesting its involvement in regulating extrinsic apoptosis. These findings highlight the potential therapeutic strategy of targeting PP5 for renal cancer treatment.
CELL CHEMICAL BIOLOGY
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
Giovanni Donato Aquaro, Carmelo De Gori, Giulia Grilli, Roberto Licordari, Andrea Barison, Giancarlo Todiere, Umberto Ianni, Matteo Parollo, Crysanthos Grigoratos, Luca Restivo, Antonio De Luca, Lorenzo Faggioni, Dania Cioni, Gianfranco Sinagra, Gianluca Di Bella, Emanuele Neri
Summary: The prognostic role of left ventricular papillary muscle abnormalities in patients with preserved LV systolic ejection fraction is unclear. This study aimed to evaluate the predictive role of LV papillary muscle abnormalities by CMR in patients with ventricular arrhythmias and preserved LVEF. The results showed that Dark-Paps is a novel prognostic marker in patients with ventricular arrhythmias and preserved ejection fraction.
EUROPEAN RADIOLOGY
(2023)
Article
Multidisciplinary Sciences
Chi Zhang, Jiangnan Xia, Xiujuan Liu, Zexing Li, Tangke Gao, Tian Zhou, Kaiwen Hu
Summary: Cell death-related genes are predictors of prognosis in cancer patients. PANoptosis, a newly discovered form of cell death, has been linked to cancer cell death and antitumor immunity. In this study, a risk prediction model for six regulators of PANoptosis was developed using mRNA gene expression and clinical information from public databases. Six genes were validated in the Gene Expression Omnibus (GEO) database. The six-gene-based model accurately estimated the prognosis of lung adenocarcinoma (LUAD) patients. Higher risk group and Cluster 2 were associated with poor prognosis and reduced expression of immune infiltrate molecules and human leukocyte antigen. qRT-PCR confirmed distinct expression of PANoptosis-related genes (PRGs) in lung cancer cells. The study also evaluated the relationship between PRGs and somatic mutations, tumor immune dysfunction exclusion, tumor stemness indices, and immune infiltration. The risk signature was utilized for nomogram construction, stratification, prediction of small-molecule drug response, somatic mutations, and chemotherapeutic response.
SCIENTIFIC REPORTS
(2023)
Article
Cell Biology
Xue Wu, Yuan Li, Wandong Zhang, Jing Zhang, Baoan Chen, Zheng Ge
Summary: This study analyzed the gene changes for diagnosis and prognosis of DLBCL using publicly available data. The results showed that high expression of RAC1 is associated with clinical stage and overall survival in DLBCL.
Article
Medicine, Research & Experimental
Chenyu Wang, Xianglai Jiang, Qiqi Zhao, Zhiyuan Xie, Hui Cai
Summary: This study found that FADD is highly expressed in multiple cancers and can be used as a diagnostic biomarker for breast cancer and colon cancer. It also has a predictive risk factor for the development of HNSC, LIHC and LUAD, and can potentially be used as a prognostic marker for these cancers.
BIOMEDICAL REPORTS
(2023)
Article
Multidisciplinary Sciences
Sofie Mossner, Doreen Manuela Floss, Juergen Scheller
Summary: Research focused on a synthetic cytokine receptor activation strategy, particularly highlighting the noncovalent trimers of the tumor necrosis factor superfamily, explored the impact of synthetic cytokine receptors on cellular signaling, showing the potential to induce pro-apoptotic and anti-apoptotic signaling through synthetic cytokine receptors.
Article
Cell Biology
Baoping Cao, Xiaochuan Guo, Lefu Huang, Bin Wang, Weixia Wang, Dong Han, Weijing Zhang, Kaili Zhong
Summary: The study demonstrated that in patients with diffuse large B-cell lymphoma, reduced expression of CDH23 due to DNA methylation was associated with poor prognosis. Functional enrichment analysis showed that CDH23 plays important roles in cancer cell growth, cell metastasis, and immunity.
Article
Multidisciplinary Sciences
Hudie Wei, Haolan Wang, Genxin Wang, Lingzhi Qu, Longying Jiang, Shuyan Dai, Xiaojuan Chen, Ye Zhang, Zhuchu Chen, Youjun Li, Ming Guo, Yongheng Chen
Summary: Mitochondrial apoptosis is controlled by BCL-2 family proteins. The tumor suppressor protein p53 triggers apoptosis through direct interactions with BCL-2 proteins. This study presents crystal structures of p53 bound to BCL-2 and reveals the molecular mechanism by which p53 regulates apoptosis by antagonizing the interaction of BCL-2 with pro-apoptotic BCL-2 family members.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Marcel Doerflinger, Boris Reljic, Joseph Menassa, Christina Nedeva, Irvin Jose, Pierre Faou, Liana Mackiewicz, Ashley Mansell, Marc Pellegrini, Richard Hotchkiss, Hamsa Puthalakath
Summary: Sepsis-induced lymphopenia and immune suppression are crucial for patient survival, yet accurate prognostic techniques or biomarkers for cell death are lacking. The novel circulating prognostic biomarker BiP may serve as a valuable tool for monitoring immune cell death during sepsis.
Article
Multidisciplinary Sciences
Pilar Lopez-Nieva, Iria Gonzalez-Vasconcellos, Laura Gonzalez-Sanchez, Maria A. Cobos-Fernandez, Sara Ruiz-Garcia, Raul Sanchez Perez, Angel Aroca, Jose Fernandez-Piqueras, Javier Santos
Summary: This study investigates the differential response of combined radiation treatments and single dose treatments. The findings highlight the different cell cycle responses and protein expression profiles, as well as the epigenetic regulation following combined radiation treatments. The results suggest that combined-dose radiation regimes could be more efficient at making cells respond to radiation.
SCIENTIFIC REPORTS
(2022)
Article
Hematology
Antonio Lahera, Pilar Lopez-Nieva, Hernan Alarcon, Jose L. Marin-Rubio, Maria A. Cobos-Fernandez, Pablo Fernandez-Navarro, Agustin F. Fernandez, Laura Vela-Martin, Isabel Sastre, Sara Ruiz-Garcia, Pilar Llamas, Jose L. Lopez-Lorenzo, Javier Cornago, Javier Santos, Jose Fernandez-Piqueras, Maria Villa-Morales
Summary: Despite frequent alterations in the JAK/STAT pathway in T-ALL/LBL, there is currently no approved therapy for patients with constitutive JAK/STAT signaling. This study identifies SOCS3 hypermethylation as a recurrent event in T-ALL/LBL and demonstrates its potential as a therapeutic target by counteracting the constitutive activation of the JAK/STAT pathway through different molecular mechanisms.
BRITISH JOURNAL OF HAEMATOLOGY
(2023)
Letter
Hematology
Antonio Lahera, Laura Vela-Martin, Pilar Lopez-Nieva, Rocio N. Salgado, Sandra Rodriguez-Perales, Raul Torres-Ruiz, Jose L. Lopez-Lorenzo, Javier Cornago, Pilar Llamas, Pablo Fernandez-Navarro, Rebeca Sanchez-Dominguez, Jose C. Segovia, Isabel Sastre, Maria A. Cobos-Fernandez, Pablo Menendez, Javier Santos, Jose Fernandez-Piqueras, Maria Villa-Morales
BRITISH JOURNAL OF HAEMATOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Maria Villa-Morales, Laura Perez-Gomez, Eduardo Perez-Gomez, Pilar Lopez-Nieva, Pablo Fernandez-Navarro, Javier Santos
Summary: The standard-of-care treatment for T-cell acute lymphoblastic leukaemia (T-ALL) achieves reasonable response rates, but relapsed or treatment-resistant patients have poor outcomes. This study explores the potential of NRF2 activation as a prognostic biomarker in T-ALL. Through analysis of transcriptomic, genomic, and clinical data, high NFE2L2 levels were found to be associated with shorter overall survival in T-ALL patients. The PI3K-AKT-mTOR pathway plays a role in the oncogenic signaling induced by NRF2 in T-ALL. High NFE2L2 levels are also associated with drug resistance in T-ALL, possibly due to NRF2-induced glutathione biosynthesis. These findings suggest that NFE2L2 levels could be a predictive biomarker for poor treatment response in T-ALL patients, offering insights for improved patient stratification and targeted therapies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Letter
Dermatology
Rosa Sacedon, M. Carmen de Arriba, Lucia Martinez-Santamaria, Rocio Maseda, Sara Herraiz-Gil, Eva Jimenez, Isabel Rosales, Lucia Quintana, Nuria Illera, Marta Garcia, Nora Butta, Ihosvany Fernandez-Bello, Su M. Lwin, Miguel Fernandez-Arquero, Carlos Leon, John A. Mcgrath, M. Angeles Vicente, Marcela del Rio, Raul de Lucas, Silvia Sanchez-Ramon, Maria Jose Escamez
BRITISH JOURNAL OF DERMATOLOGY
(2023)
Review
Cell Biology
Jose L. Marin-Rubio, Ishier Raote, Joseph Inns, Carol Dobson-Stone, Neil Rajan
DISEASE MODELS & MECHANISMS
(2023)
Article
Biochemical Research Methods
Amy L. George, Frances R. Sidgwick, Jessica E. Watt, Mathew P. Martin, Matthias Trost, Jose Luis Marin-Rubio, Maria Emilia Duenas
Summary: Thermal proteome profiling (TPP) is a powerful approach to studying the interactions of therapeutic molecules with proteins. Isobaric tandem mass tags (TMTs) are commonly used in TPP analysis, but data-independent acquisition (DIA) approaches offer higher sensitivity and protein coverage with reduced costs. In this study, different DIA-based label-free quantification approaches were compared to TMT-DDA for thermal shift quantitation, and it was found that DIA approaches are a cost-effective alternative.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Antonio Lahera, Laura Vela-Martin, Pablo Fernandez-Navarro, Pilar Llamas, Jose L. Lopez-Lorenzo, Javier Cornago, Javier Santos, Jose Fernandez-Piqueras, Maria Villa-Morales
Summary: This study identified the JAK3(Q988P) mutation in a T-ALL patient, which plays an oncogenic role in leukemogenesis and confers resistance to conventional treatment as well as ruxolitinib. These findings highlight the importance of evaluating this mutation for guiding personalized therapeutic strategies.
MOLECULAR CARCINOGENESIS
(2023)
Article
Multidisciplinary Sciences
Isabel Tundidor, Marta Seijo-Vila, Sandra Blasco-Benito, Maria Rubert-Hernandez, Sandra Adamez, Clara Andradas, Sara Manzano, Isabel Alvarez-Lopez, Cristina Sarasqueta, Maria Villa-Morales, Carmen Gonzalez-Lois, Esther Ramirez-Medina, Belen Almoguera, Antonio J. Sanchez-Lopez, Laura Bindila, Sigrid Hamann, Norbert Arnold, Christoph Roecken, Ignacio Heras-Murillo, David Sancho, Gema Moreno-Bueno, Maria M. Caffarel, Manuel Guzman, Cristina Sanchez, Eduardo Perez-Gomez
Summary: Clinical management of breast cancer metastasis is an unmet need. This study identifies fatty acid amide hydrolase (FAAH) as a predictor of prognosis in patients with luminal BC and a potential therapeutic target for preventing tumor progression and lung metastasis.
NATURE COMMUNICATIONS
(2023)
Article
Food Science & Technology
Sara Herraiz-Gil, Maria del Carmen de Arriba, Maria J. Escamez, Carlos Leon
Summary: The integration of multi-omics data enables comprehensive analysis of complex matrixes at the molecular level. Modern analytical techniques have been essential in advancing food science and understanding the relationships between food, health, and disease. However, there are still challenges in integrating all these data. This review provides an up-to-date critical overview of multi-omic approaches, their advantages, limitations, and recent research in the field of Foodomics.
CURRENT OPINION IN FOOD SCIENCE
(2023)
Article
Chemistry, Medicinal
Jose Luis Marin-Rubio, Rachel E. Peltier-Heap, Maria Emilia Duenas, Tiaan Heunis, Abeer Dannoura, Joseph Inns, Jonathan Scott, A. John Simpson, Helen J. Blair, Olaf Heidenreich, James M. Allan, Jessica E. Watt, Mathew P. Martin, Barbara Saxty, Matthias Trost
Summary: In this study, a label-free cellular phenotypic drug discovery assay was developed to identify anti-inflammatory drugs in acute myeloid leukemia cells. The results showed that nilotinib, but not imatinib, could block inflammatory responses. Further investigation revealed that nilotinib binds to p38α and inhibits the p38α-MK2/3 signaling axis, thereby suppressing pro-inflammatory cytokine expression, cell adhesion, and innate immunity markers in activated monocytes derived from AML.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Meeting Abstract
Gastroenterology & Hepatology
Ruth Walker, Michalina Zatorska, Jose Luis Marin-Rubio, Matthias Trost, Maria Emilia Duenas, Olivier Govaere
JOURNAL OF HEPATOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Pilar Lopez-Nieva, Pablo Fernandez-Navarro, Maria Angeles Cobos-Fernandez, Iria Gonzalez-Vasconcellos, Raul Sanchez Perez, Angel Aroca, Jose Fernandez-Piqueras, Javier Santos
Summary: This study investigates the differential expression pattern of circRNAs in different stages of human thymocyte development and provides new circRNA-miRNA-mRNA networks capable of controlling the expression of genes involved in T-cell differentiation in the thymus.
