4.2 Article

Development of nuclear, chloroplast and mitochondrial SNP markers for Khaya sp.

Journal

CONSERVATION GENETICS RESOURCES
Volume 8, Issue 3, Pages 283-297

Publisher

SPRINGER
DOI: 10.1007/s12686-016-0557-4

Keywords

Khaya; SNP; Tropical tree; MassARRAY (R) iPLEX (TM); Genetic differentiation

Funding

  1. German Federal Ministry of Food and Agriculture (BMEL)
  2. International Tropical Timber Organization (ITTO)
  3. Prince Albert II of Monaco Foundation
  4. CGIAR Research Program on Forests, Trees and Agroforestry (FTA)
  5. Service Cooperation et d'Action culturelle de l'Ambassade de France au Congo (SCAC)
  6. Fondation international pour la Science
  7. Centre de cooperation International en Recherche Agronomique pour le Developement (CIRAD)
  8. Double Helix Tracking Technologies

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100 SNP and one Indel markers were developed for Khaya using a combination of restriction associated DNA sequencing and low coverage MiSeq genome sequencing. The marker set was successfully used to genotype a set of 1919 individuals, representing 5 Khaya species from 18 African countries, using MassARRAY(A (R))iPLEX (TM) genotyping.

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