Journal
BIOMEDICAL OPTICS EXPRESS
Volume 7, Issue 10, Pages 4263-4274Publisher
OPTICAL SOC AMER
DOI: 10.1364/BOE.7.004263
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Funding
- Dutch Technology Foundation STW [12154]
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Photobleaching is a major factor limiting the observation time in fluorescence microscopy. We achieve photobleaching reduction in structured illumination microscopy (SIM) by locally adjusting the illumination intensities according to the sample. Adaptive SIM is enabled by a digital micro-mirror device (DMD), which provides a projection of the grayscale illumination patterns. We demonstrate a reduction in photobleaching by a factor of three in adaptive SIM compared to the non-adaptive SIM based on a spot grid scanning approach. Our proof-of-principle experiments show great potential for DMD-based microscopes to become a more useful tool in live-cell SIM imaging. (C) 2016 Optical Society of America
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