Journal
BIOINTERPHASES
Volume 11, Issue 2, Pages -Publisher
AMER INST PHYSICS
DOI: 10.1116/1.4944830
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Funding
- Center for Synthetic Biology bioSYNergy - UCPH Excellence Program for Interdisciplinary Research
- Swedish Research Council
- Plant Power: Light driven synthesis of complex terpenoids using cytochrome P450s - Innovation Fund Denmark
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In this review, the authors discuss the challenges of studying supported lipid bilayers (SLBs) deposited by vesicle fusion in terms of (1) evaluating SLB formation and quality using quartz crystal microbalance with dissipation and (2) analyzing the composition and asymmetry of SLBs composed by lipid mixtures using complementary surface sensitive techniques. An overview of the literature is presented and the inconsistencies on this topic are discussed with the objective to expand beyond simple lipid compositions and set the basis for forming and analyzing SLBs of complex natural lipid extracts formed via the vesicle fusion method. The authors conclude by providing some guidelines to successfully form SLBs of complex lipid mixtures including natural extracts. (C) 2016 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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