Article
Chemistry, Physical
Katarzyna Gajos, Andrzej Budkowski, Panagiota Petrou, Sotirios Kakabakos
Summary: The biosensor interface is crucial for biosensing devices and point-of-need platforms. Traditional biosensor response cannot differentiate between different surface modifications, while Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS) can provide microanalysis and chemical imaging to solve this problem. ToF-SIMS allows control over molecular orientation and conformation, and can be used to optimize biosensor surface composition and biofunctionalization protocols. It provides more accurate characterization of molecular composition and allows verification of applied protocols and optimization of assay conditions. Furthermore, ToF-SIMS imaging can be used to study biomolecules printed into microarrays, biosensor arrays or separate biosensor components on a chip. It is hoped that these experimental developments using ToF-SIMS analysis methods will inspire future research in understanding complex surface phenomena.
APPLIED SURFACE SCIENCE
(2022)
Article
Chemistry, Physical
Aram Hong, Sang Ju Lee, Chang Min Choi, Ji Young Baek, Myoung Choul Choi
Summary: By measuring the surface potential of a polymer film, the effect of gas on charge compensation was investigated. The experimental results showed that the type of injected gas may impact the surface charging of the sample.
APPLIED SURFACE SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Hung-Ming Chang, Hsing-Chun Lin, Hsin-Lin Cheng, Chih-Kai Liao, To-Jung Tseng, Ting-Yi Renn, Chyn-Tair Lan, Li-You Chen
Summary: The study found that early-life sleep deprivation can have long-term effects, such as disruption in ion distribution, interruption of neurotransmitter and receptor expressions, decreases in antioxidant enzyme levels, and impairment of signaling pathways leading to neuronal death in hippocampal regions. However, administration of melatonin can prevent these effects and increase the number of neurons in the hippocampus.
Article
Endocrinology & Metabolism
Linshu Wang, Kiran Chaudhari, Ali Winters, Yuanhong Sun, Ran Liu, Shao-Hua Yang
Summary: This study optimized a biopsy punching method to analyze the region-specific glucose metabolism in rat and mouse brains. The results showed that the cerebellum had a more quiescent phenotype of glucose metabolism compared to other brain regions and had higher AMPK activation. Additionally, the study found that rodent brains have relatively low mitochondrial oxidative phosphorylation efficiency and a significant percentage of respiration is linked to proton leak.
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
(2022)
Article
Materials Science, Multidisciplinary
Dexuan Yan, Xinlei Cao, Ke Shen
Summary: This study investigated the purification mechanism of polycrystalline graphite by comparing IG-11 graphite with IG-110 nuclear grade graphite. The analysis revealed that metallic impurities in IG-11 were primarily segregated within graphite porosities, while IG-110 demonstrated a significant reduction in impurities. This research contributes to the development of innovative graphite purification techniques for greater purity and stronger oxidation resistance.
JOURNAL OF NUCLEAR MATERIALS
(2024)
Article
Biochemistry & Molecular Biology
Emily J. Camm, Christine M. Cross, Andrew D. Kane, Jane L. Tarry-Adkins, Susan E. Ozanne, Dino A. Giussani
Summary: Chronic fetal hypoxia during pregnancy can lead to long-term structural and functional impairments in the adult offspring's brain, particularly affecting neuronal number, vascular and synaptic density in the hippocampus, and memory function. Maternal supplementation with vitamin C during hypoxic pregnancy can protect against oxidative stress in the placenta and prevent adverse effects on hippocampal atrophy and memory loss in the adult offspring, indicating a potential therapeutic strategy.
Article
Engineering, Environmental
Liuqin Huang, Qun Yu, Wen Liu, Jungang Wang, Wenxiao Guo, Endong Jia, Qiang Zeng, Ruijun Qin, Jianqiu Zheng, Kirsten S. Hofmockel, Hailiang Dong, Hongchen Jiang, Zihua Zhu
Summary: This study provides direct and detailed molecular evidence for the preferential adsorption of microbial carbon over humic substances on clay minerals during iron redox processes. The results show that pre-adsorbed humic substances can inhibit the structural modification of clay minerals by microbial Fe(III) reduction, leading to a decreased adsorption of microbial carbon. The study also highlights the unique role of ToF-SIMS in understanding complex organic-mineral-microbe interactions.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2021)
Article
Chemistry, Analytical
David Majer, Matjaz Finsgar
Summary: This study developed a square-wave adsorption stripping voltammetry (SWAdSV) method for the simultaneous determination of epinephrine (EP) and uric acid (UA) using a poly(L-cysteine) (pLC) film modified screen-printed carbon electrode (pLC-SPCE). The method showed low detection and quantification limits, wide linear concentration range, high accuracy and precision. It also demonstrated good selectivity towards interferents and successfully analyzed pharmaceutical samples and human urine.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Physical
Camille Trinidad, Jolanta Swiatowska, Sandrine Zanna, Antoine Seyeux, Dimitri Mercier, Remi Viroulaud, Philippe Marcus
Summary: XPS and ToF-SIMS analysis revealed that surface modifications induced by H2SO4, HNO3, and a mixture of both acids resulted in significant copper enrichment at the oxide/metal interface of AA-2024-T3. This enrichment was mainly attributed to selective dissolution of less noble elements, leading to dealloying of intermetallic particles.
APPLIED SURFACE SCIENCE
(2021)
Article
Biochemical Research Methods
Zhiyuan Yuan, Qiming Zhou, Lesi Cai, Lin Pan, Weiliang Sun, Shiwei Qumu, Si Yu, Jiaxin Feng, Hansen Zhao, Yongchang Zheng, Minglei Shi, Shao Li, Yang Chen, Xinrong Zhang, Michael Q. Zhang
Summary: Spatial metabolomics using SEAM method combines high-spatial-resolution imaging mass spectrometry and computational algorithms to explore tissue organization and heterogeneity at the single-cell level. It has been successfully applied in mouse and human liver tissues, revealing metabolic zonation patterns in the liver and subpopulations of hepatocytes with special metabolic features associated with fibrotic niches. SEAM is a powerful platform for analyzing spatially resolved nuclear metabolomics profiling.
Article
Chemistry, Analytical
Tanja Bien, Sebastian Bessler, Klaus Dreisewerd, Jens Soltwisch
Summary: Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has the potential to visualize the spatial distribution of biomolecules in cell cultures and tissue sections with high resolution, but sample preparation is crucial. A study found that a robust sample preparation protocol can preserve cell morphology and molecular integrity, contributing to the study of cell-to-cell heterogeneities at a molecular level. Advancements in single-cell MALDI imaging could benefit from these protocols.
ANALYTICAL CHEMISTRY
(2021)
Review
Clinical Neurology
Stefan Stamenkovic, Yuandong Li, Jack Waters, Andy Shih
Summary: The increasing socio-economic burden of Alzheimer disease (AD) and AD-related dementias has created a pressing need to define targets for therapeutic intervention. Animal models that recapitulate features of AD/AD-related dementias may provide mechanistic insight because microvascular pathology can be studied as it develops in vivo. Recent advances in in vivo optical and ultrasound-based imaging of the rodent brain facilitate this goal by providing access to deeper brain structures, including white matter and hippocampus, which are more vulnerable to injury during cerebrovascular disease.
Article
Chemistry, Analytical
David Majer, Matjaz Finsgar
Summary: This study developed a non-enzymatic sensor for the determination of the organophosphorus pesticide methyl parathion. By optimizing the experimental conditions and utilizing the multiple standard addition methodology, the accuracy and precision of the detection method were significantly improved.
MICROCHEMICAL JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Klaudia Szafran, Malgorzata Jurak, Robert Mroczka, Agnieszka Ewa Wiacek
Summary: This paper aims to characterize multi-component films containing chitosan (Ch), phospholipid 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), immunosuppressant cyclosporine A (CsA) and/or antioxidant lauryl gallate (LG) for developing PET coatings. The films were prepared using the Langmuir-Blodgett technique and their nanostructure, surface chemistry, and wettability were determined. The results demonstrate the relationship between surface properties and component ratio and provide insights into the coating organization and interactions at the molecular level. These organized layers can enhance the biocompatibility of biomaterials and offer a basis for further research on the immune system response and physicochemical properties of biomaterials.
Article
Electrochemistry
Junsoo Han, Shova Neupane, Luntao Wang, Antoine Seyeux, Lorena Klein, Sandrine Zanna, Dimitri Mercier, Vincent Maurice, Philippe Marcus
Summary: The effect of high-temperature annealing with hydrogen gas surface preparation on a binary polycrystalline Ni-20 at.% Cr alloy was compared with conventional mechanical grinding surface preparation. The results showed that the annealed surface formed a thinner and less homogeneous inner barrier layer, leading to an oxidation peak in the corrosion measurement. However, despite this high oxidation peak, the annealed surface exhibited a more corrosion resistant inner layer formation compared to the mechanically ground surface, attributed to increased Cr enrichment caused by selective Ni dissolution.
ELECTROCHIMICA ACTA
(2023)
Article
Biochemistry & Molecular Biology
Ibrahim Kaya, Eva Jennische, Johan Dunevall, Stefan Lange, Andrew G. Ewing, Per Malmberg, Ahmet Tarik Baykal, John S. Fletcher
ACS CHEMICAL NEUROSCIENCE
(2020)
Article
Biochemistry & Molecular Biology
Mai Hoang Philipsen, Nhu T. N. Phan, John Stephen Fletcher, Andrew G. Ewing
ACS CHEMICAL NEUROSCIENCE
(2020)
Article
Biochemistry & Molecular Biology
Ibrahim Kaya, Eva Jennische, Stefan Lange, Ahmet Tarik Baykal, Per Malmberg, John S. Fletcher
JOURNAL OF NEUROCHEMISTRY
(2020)
Article
Chemistry, Analytical
Sanna Samfors, John S. Fletcher
ANNUAL REVIEW OF ANALYTICAL CHEMISTRY, VOL 13
(2020)
Article
Biophysics
Kelly Dimovska Nilsson, Noora Neittaanmaki, Oscar Zaar, Tina B. Angerer, John Paoli, John S. Fletcher
Article
Biochemical Research Methods
Ibrahim Kaya, Sanna Samfors, Malin Levin, Jan Boren, John S. Fletcher
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2020)
Article
Chemistry, Physical
Kelly Dimovska Nilsson, Johan Granden, Anne Farewell, John S. Fletcher
Summary: ToF-SIMS was used to analyze the chemical changes in the bacterial envelope of a fabF knock-out Escherichia coli strain. The results showed a substantial depletion of FA(18:1) in the fabF mutant during logarithmic growth, as well as a reduction in FA(cp19:0) during stationary phase. Additionally, the wild type strain exhibited a reduction in cyclopropanation at lower culture temperatures and a 'thickening' of the lipid A layer on the surface of the bacteria during stationary phase.
SURFACE AND INTERFACE ANALYSIS
(2021)
Article
Neurosciences
Sarah Threlfell, Amir Saeid Mohammadi, Brent J. Ryan, Natalie Connor-Robson, Nicola J. Platt, Rishi Anand, Florence Serres, Trevor Sharp, Nora Bengoa-Vergniory, Richard Wade-Martins, Andrew Ewing, Stephanie J. Cragg, Katherine R. Brimblecombe
Summary: This study investigated the impact of human alpha-synuclein on DAT function in a transgenic mouse model of early PD. The results show that alpha-synuclein can promote striatal DAT function, which is regulated by extracellular cholesterol.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2021)
Article
Engineering, Mechanical
Georgios Birmpilis, Amir Saeid Mohammadi, Julie Villanova, Elodie Boller, Edward Ando, Jelke Dijkstra
Summary: The three-dimensional fabric in natural sensitive clay is quantified using high resolution nano and microtomographies, scanning electron microscopy, and dynamic light scattering. While individual platelet-shaped clay particles cannot be segmented for quantitative analysis, a wide range of particle sizes was detected, and it was found that smaller particles deviate in orientation compared to larger particles.
JOURNAL OF ENGINEERING MECHANICS
(2022)
Article
Biochemical Research Methods
Tina B. Angerer, Jerome Bour, Jean-Luc Biagi, Eugene Moskovets, Gilles Frache
Summary: Mass spectrometry imaging is a technique used to localize and identify lipids in tissue samples. By utilizing an AP-MALDI source coupled to an Orbitrap Elite, lipid locations and structures can be determined with high mass resolution spectra and cellular spatial resolution. Different MALDI matrices and treatment protocols have varying effects on the lipid coverage and signal intensity in positive and negative ion modes.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2022)
Article
Dermatology
C. Jacques, S. Rattier, P. Bianchi, T. B. Angerer, G. Frache, L. Cattuzzato, L. Perrin, A. Villaret, V Duran, J. Noharet, A. Rouquier, S. Bessou-Touya, C. Bidan, H. Duplan
Summary: This study developed two formulations that resemble the lipid organization of the stratum corneum and can penetrate the skin without disrupting the lipid lamella organization. These formulations increased skin hydration in clinical subjects and could potentially alleviate sensitive skin.
JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY
(2022)
Article
Chemistry, Multidisciplinary
C. Jacques, F. Crepel, D. El Assad, T. B. Angerer, J. Bour, C. Jeanjean-Miquel, D. Redoules, D. Bacqueville, F. Pamelard, S. Bessou-Touya, G. Frache, H. Duplan
Summary: The study found that sun filters in sunscreen mainly remain on the surface of the skin after topical application, with minimal penetration into deeper layers. This finding was confirmed using mass spectrometry imaging techniques, which can visualize the distribution of compounds in the skin.
JOURNAL OF CONTROLLED RELEASE
(2022)
Article
Chemistry, Analytical
Charlotte B. A. Stoffels, Tina B. Angerer, Herve Robert, Nathalie Poupin, Laila Lakhal, Gilles Frache, Muriel Mercier-Bonin, Jean-Nicolas Audinot
Summary: This study conducted a comprehensive lipidomic analysis on the liver of PFOA-exposed mice using three mass spectrometry techniques. It was found that over 350 hepatic lipids were significantly impacted by PFOA exposure, with phosphatidylethanolamine (PE), phosphatidylcholine (PC), and triacylglycerol (TG) being the most affected lipid classes. The multi-modal mass spectrometry analysis revealed the lipidomic impact of PFOA in the mouse liver and provided new opportunities in toxicology research.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Inci Barut, Xiulan He, Erol Sener, Sanna Samfors, Andrew G. Ewing, John S. Fletcher
Summary: The abnormal functioning of proteasome is associated with crucial pathological conditions, such as neurodegeneration. However, the complex underlying causes at the cellular level are still unclear, and there are conflicting reports on the neuroprotective and neurodegenerative effects of proteasomal inhibitors like lactacystin, which are used as models for neurodegenerative diseases. The conflicting results may be due to different doses of lactacystin. Hence, a dose-dependent study was conducted to investigate the effects of lactacystin on cell membrane lipid profile and exocytosis dynamics.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Engineering, Environmental
Cecilia Burzio, Amir Saeid Mohammadi, Per Malmberg, Oskar Modin, Frank Persson, Britt-Marie Wilen
Summary: The presence of pharmaceuticals in aquatic environments poses challenges to water quality worldwide, including harmful effects on both ecological and human health, development of antibiotic resistance, and disruption of endocrine function in aquatic organisms. This study successfully used time-of-flight secondary ion mass spectrometry imaging to investigate the distribution of pharmaceuticals and endogenous molecules in the complex biological matrix of biofilms used in wastewater treatment. The findings showed that certain pharmaceuticals were located in specific sites within the biofilm, with colocalization mainly with phosphocholine lipids. This approach provides a powerful strategy for visualizing the chemical composition of biofilms and elucidating the mechanisms behind the removal of pharmaceuticals and antimicrobials in biological wastewater treatment.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)