Article
Oncology
Heather M. Wojcik, Harold N. Lovvorn III, Melinda Hollingshead, Janene Pierce, Howard Stotler, Andrew J. Murphy, Suzanne Borgel, Hannah M. Phelps, Hernan Correa, Alan O. Perantoni
Summary: Wilms Tumor (WT) is the most common pediatric kidney cancer. Researchers have successfully cultured WT stem cells and established a cell model for studying WT, which is important for the research on drug efficacy and resistance.
FRONTIERS IN ONCOLOGY
(2023)
Review
Cell Biology
Navin Gupta, Ryuji Morizane
Summary: The development of kidney organoid technology has revitalized the field of kidney developmental biology, allowing for the generation of nephron and collecting duct kidney organoids through directed differentiation protocols. However, there are limitations to current methods that need to be addressed for future improvements.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Manar Abboud Asleh, Mira Zaher, Jad Asleh, Julian Jadon, Lihi Shaulov, Ronit Yelin, Thomas M. Schultheiss
Summary: This study investigates the process of mesenchymal-epithelial transition (MET) in the chick embryo lateral mesoderm. Prior to MET initiation, mesenchymal cells exhibit non-polarized distribution of multiple polarity markers, albeit not aPKC. We present a stepwise model for MET, comprising polarization, 3D-rosette, and epithelialization stages.
DEVELOPMENTAL CELL
(2023)
Article
Agriculture, Dairy & Animal Science
Ejaz R. Dar, Mudasir B. Gugjoo, Moien Javaid, Shahid Hussain, Mujeeb R. Fazili, Kuldeep Dhama, Taha Alqahtani, Ali M. Alqahtani, Riaz A. Shah, Talha Bin Emran
Summary: The study evaluates the influence of ambient temperature, donor physiological status, and tissue source on sheep mesenchymal stem cells. Results show that adipose tissue-derived MSCs from full term pregnant female sheep exhibit higher potential for proliferation and differentiation compared to male sheep-derived MSCs. Cryopreserved MSCs have similar characteristics to fresh cells. Therefore, it is important to consider tissue type and donor physiological status when applying MSCs in clinical settings.
Article
Cell & Tissue Engineering
Zhuoran Yu, Sihan Zhou, Minli Liu, Lijun Chen, Xue Zhang, Xu Yang, Hongdi Cui, Chaoqian Jiang, Yanjiao Lv, Tingsheng Yan, Qingran Kong, Zhonghua Liu
Summary: This study successfully derived and maintained stable porcine pluripotent stem cells (pLCDMs) using the LCDM culture system. Transcriptome analysis revealed the unique molecular characteristics and similarities/differences with human and mouse EPSCs. Furthermore, two small molecules in the culture system were found to play a crucial role in promoting trophoblast lineage differentiation potential of pLCDMs.
Article
Cell & Tissue Engineering
Reinhard Dolp, Gertraud Eylert, Christopher Auger, Ayesha Aijaz, Yufei Andy Chen, Saeid Amini-Nik, Alexandra Parousis, Andrea-Kaye Datu, Marc G. Jeschke
Summary: This study compared the cell characteristics of burned skin-derived mesenchymal stromal/stem cells with umbilical cord mesenchymal stromal/stem cells, revealing differences in mesenchymal differentiation capacity and cytokine secretion. However, other aspects like population doubling time, colony formation, cell proliferation, and immune markers showed no significant differences, indicating potential for cell therapy and skin regeneration.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Biochemistry & Molecular Biology
Songyue Lou, Yongtao Duan, Huizong Nie, Xujie Cui, Jialing Du, Yongfang Yao
Summary: Mesenchymal stem cells (MSCs) have immunomodulatory functions and transplantation potential, making them suitable for the treatment of various diseases. They have broad clinical application prospects in tissue regeneration, immunomodulation, and oncology.
Article
Engineering, Multidisciplinary
Zeinab Hormozi-Moghaddam, Manijhe Mokhtari-Dizaji, Mohammad-Ali Nilforoshzadeh, Mohsen Bakhshandeh
Summary: This study found that a mechanical index of 0.20 promoted collagen I expression and cell proliferation through low-intensity ultrasound stimulation.
Article
Multidisciplinary Sciences
Leticia de Barros Sene, Wellerson Rodrigo Scarano, Adriana Zapparoli, Jose Antonio Rocha Gontijo, Patricia Aline Boer
Summary: This study found that gestational low-protein intake can affect renal development in offspring by altering miRNA and predicted target gene expression levels in the kidney, leading to a reduction in nephron number. Specifically, there were decreased cell numbers and changes in immunoreactivity of related proteins observed in the metanephros.
Review
Biochemistry & Molecular Biology
George Kolios, Vasilis Paspaliaris
Summary: Mesenchyme Stem Cells (MSCs) are extensively used in regenerative medicine and have shown positive effects in treating various diseases through their secretion of growth factors. They have the potential to be efficacious tools in regenerative medicine.
Article
Cell Biology
Hong Hu, Yufeng Duan, Kun Wang, Huancheng Fu, Yuansong Liao, Tianshu Wang, Ziwei Zhang, Fanchen Kang, Baiquan Zhang, Haiying Zhang, Fangjun Huo, Yike Yin, Guoqing Chen, Hongbo Hu, Haoyang Cai, Weidong Tian, Zhonghan Li
Summary: This study investigates the cellular dynamics during tooth development and reveals the contribution of dental niche cells to tooth organogenesis. The research also identifies a previously unappreciated group of migratory cells potentially involved in dental papilla formation.
Article
Biochemistry & Molecular Biology
Shalini Raik, Prakshi Sharma, Saroj Kumar, Vidya Rattan, Ashim Das, Navin Kumar, Radhika Srinivasan, Shalmoli Bhattacharyya
Summary: Mesenchymal stem/stromal cell (MSC) spheroids generated in a three-dimensional (3D) culture system can better maintain stem cell characteristics and mimic the in vivo behavior of cells and tissue. In this study, the spheroids generated in ultra-low attachment flasks were extensively characterized and compared with monolayer culture derived cells. Dental pulp stem cells (DPSCs) cultured under ultra-low attachment conditions formed compact and well-organized multicellular spheroids, exhibiting superior stemness, differentiation, and regenerative abilities compared to monolayer cells. The results suggest that scaffold-free 3D culture efficiently preserves DPSCs intrinsic properties and functionality, making it a feasible and efficient method for generating robust spheroids for various in vitro and in vivo therapeutic applications.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Yu Guo, Huan Zhu, Xiangchen Li, Caiyun Ma, Tingting Sun, Yuanyuan Wang, Chunjing Wang, Weijun Guan, Changqing Liu
Summary: Reversine has the ability to reverse cell differentiation and suppress tumor growth, inducing multinucleated cells through cytokinesis failure, reversing cell differentiation, and promoting cell apoptosis through the mitochondria mediated pathway.
SCIENTIFIC REPORTS
(2021)
Review
Cell Biology
Yufeng Shang, Haotong Guan, Fuling Zhou
Summary: Umbilical cord mesenchymal stem cells (UC-MSCs) have the characteristics of inhibiting tumor cell proliferation and migration, supporting hematopoietic microenvironment, and suppressing the immune system, showing great potential in the treatment of hematologic diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Daisuke Yamada, Tomoka Takao, Masahiro Nakamura, Toki Kitano, Eiji Nakata, Takeshi Takarada
Summary: This study found that high pluripotent cell density suppresses the expression of PRRX1 mRNA and tdTomato during the induction of PRRX1(+) LBM-like cells from hPSCs. Through RNA sequencing and flow cytometry, CD44(high) CD140B(high) CD49f(-) were identified as the surface antigens for PRRX1-tdTomato(+) LBM-like cells. These results were validated in different hPSC lines.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
