Article
Biochemistry & Molecular Biology
Yang Wang, Randy Y. C. Poon
Summary: The anti-apoptotic protein MCL1 is gradually degraded during mitotic arrest, and the initial level of MCL1 is determined by the mitochondrial ubiquitin ligase MARCH5. Knockout of MARCH5 increases mitotic MCL1 but paradoxically enhances mitotic apoptosis in a BAK-dependent manner. MARCH5 regulates mitotic apoptosis through mechanisms independent of MCL1, including mitochondrial maintenance.
CELL DEATH AND DIFFERENTIATION
(2023)
Article
Oncology
Carolin Golla, Mayas Bilal, Annika Dwucet, Nicolas Bader, Jenson Anthonymuthu, Tim Heiland, Maximilian Pruss, Mike-Andrew Westhoff, Markus David Siegelin, Felix Capanni, Christian Rainer Wirtz, Richard Eric Kast, Marc-Eric Halatsch, Georg Karpel-Massler
Summary: The combination of photodynamic therapy and ABT-263 exhibited synergistic antineoplastic effects on glioblastoma cells, suggesting a potential benefit for treating this disease. The study aimed to enhance the biological effects of 5-aminolevulinic acid-based photodynamic therapy by inhibiting Bcl-2/Bcl-xL proteins in different glioblastoma models. Further investigation is warranted based on the promising results.
Article
Biochemistry & Molecular Biology
Laura Ravazzolo, Sara Trevisan, Silvia Iori, Cristian Forestan, Mario Malagoli, Silvia Quaggiotti
Summary: In this study, RNA sequencing was used to identify the main molecular signatures of maize root response to nitrate, distinguishing between nitric oxide-dependent and independent pathways. Six clusters of transcripts were identified based on their responsiveness to nitric oxide and regulation by nitrate provision, providing insight into the overall root response to nitrate.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Leticia Ramirez-Silva, Gloria Hernandez-Alcantara, Carlos Guerrero-Mendiola, Martin Gonzalez-Andrade, Adela Rodriguez-Romero, Annia Rodriguez-Hernandez, Alan Lugo-Munguia, Paul A. Gomez-Coronado, Cristina Rodriguez-Mendez, Alicia Vega-Segura
Summary: The presence of different amino acid residues in pyruvate kinases from eukarya and bacteria contributes to their K+-dependent and -independent activities. Residues 117 and 120 play a crucial role in the differences between these two types of enzymes, with T120 being important in the conformation of K+-dependent enzymes. The study reveals two different mechanisms for the catalysis of this reaction.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Mohamed Chamlali, Sana Kouba, Lise Rodat-Despoix, Luca Matteo Todesca, Zoltan Pethoe, Albrecht Schwab, Halima Ouadid-Ahidouch
Summary: Orai3 regulates breast cancer cell migration through a Ca2+-dependent modulation of cell adhesive capacities and a Ca2+-independent regulation of the actin cytoskeleton via focal adhesion kinase (FAK).
Article
Neurosciences
Sidhanth Chandra, Antonio Di Meco, Hemraj B. Dodiya, Jelena Popovic, Leah K. Cuddy, Ian Q. Weigle, Xiaoqiong Zhang, Katherine Sadleir, Sangram S. Sisodia, Robert Vassar
Summary: In this study, the researchers found that antibiotic treatment alters the gut microbiome and reduces amyloid beta plaques and proinflammatory microglial phenotype in male APPPS1-21 mice. However, the effect of this alteration on astrocytes and astrocyte-microglia communication in the context of amyloidosis has not been examined.
MOLECULAR NEURODEGENERATION
(2023)
Article
Biochemistry & Molecular Biology
Kyunghyun Yoo, Hye-Hyeon Yun, Soon-Young Jung, Jeong-Hwa Lee
Summary: This study demonstrated that KRIBB11 substantially inhibits the growth of A172 glioma cells by inducing apoptosis through decreasing anti-apoptotic protein MCL-1 levels. Furthermore, the stabilization of MULE upon KRIBB11 treatment is essential for the degradation of MCL-1 and subsequent induction of apoptosis in A172 cells.
Review
Immunology
Wenjing Xuan, Maciej S. Lesniak, Charles David James, Amy B. Heimberger, Peiwen Chen
Summary: This article discusses how intrinsic alterations in tumor cells affect the biology of immune cells and how these immune cells support tumor progression. Studying the relationship between GBM and GAM can reveal the molecular basis of GBM tumorigenesis and lead to new treatment approaches to improve patient outcomes.
TRENDS IN IMMUNOLOGY
(2021)
Article
Endocrinology & Metabolism
Jia Gu, Yongqing Ye, Yan Wang, Wenjian Zhan, Rutong Yu
Summary: The study found that long non-coding RNA LINC01094 is highly expressed in glioblastoma. STAT3 can enhance the transcription activity of LINC01094, which, in turn, regulates the expression of UBE2N through binding miR-545-3p, thus modulating the proliferation, apoptosis, and radiosensitivity of glioblastoma. This finding provides a potential target for glioblastoma treatment.
JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS
(2023)
Review
Biochemistry & Molecular Biology
Jinhui Wang, Hejia Ni, Lin Chen, Jianan Zou, Chunyan Liu, Qingshan Chen, Pascal Ratet, Dawei Xin
Summary: This review summarizes the latest advances in understanding the interaction between secreted effectors and soybean feedback mechanism, uncovering the conserved and special signaling pathways induced by pathogenic soybean cyst nematode, Pseudomonas, Xanthomonas, as well as symbiotic rhizobium.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Pharmacology & Pharmacy
Qiu Sun, Mengyuan Huang, Yuquan Wei
Summary: This article mainly describes the importance of non-methylation reactions of SAM-dependent enzymes in biosynthesis, compares the structural and mechanistic similarities and distinctions between SAM-dependent MTases and non-methylating SAM-dependent enzymes, summarizes the reactions catalyzed by these enzymes, and discusses the structural conservation and catalytical diversity of class I-like non-methylating SAM-dependent enzymes. It also proposes a possibility in enzyme evolution and suggests future perspectives for enzyme-mediated chemistry and biotechnology for the development of new methods for drug synthesis.
ACTA PHARMACEUTICA SINICA B
(2021)
Article
Environmental Sciences
Yuanzhou Xu, Xinyue Zhang, Zhuoliang Xiao, Fei Wang, Mingyue He, Yunyun Li, Ting Che, Yajuan Li, Xiayun Zang, Huixin Li, Feng Hu, Li Xu
Summary: Plant growth and environmental concentration have an impact on the uptake of fluoranthene by plants, yet their specific contributions remain poorly evaluated. In this study, ryegrass was exposed to different concentrations of fluoranthene to investigate its uptake mechanism. The results showed that fluoranthene absorption capacity was stronger in the roots than in the leaves. The bioconcentration and translocation factors of fluoranthene were highest at a concentration of 5 mg/L. Plant growth and substance synthesis indices exhibited a similar pattern to the bioconcentration factor, while antioxidant enzyme activities showed a concentration-dependent response.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Biochemistry & Molecular Biology
Lorela Ciraku, Zachary A. Bacigalupa, Jing Ju, Rebecca A. Moeller, Giang Le Minh, Rusia H. Lee, Michael D. Smith, Christina M. Ferrer, Sophie Trefely, Luke T. Izzo, Mary T. Doan, Wiktoria A. Gocal, Luca D'Agostino, Wenyin Shi, Joshua G. Jackson, Christos D. Katsetos, Kathryn E. Wellen, Nathaniel W. Snyder, Mauricio J. Reginato
Summary: This study identifies a novel mechanism in which OGT regulates acetate-dependent acetyl-CoA and lipid production by regulating phosphorylation of ACSS2 by CDK5. The OGT/CDK5/ACSS2 pathway may be a potential target for treating altered metabolic dependencies in brain tumors.
Article
Multidisciplinary Sciences
Shengjun Tan, Huijing Ma, Jinbo Wang, Man Wang, Mengxia Wang, Haodong Yin, Yaqiong Zhang, Xinying Zhang, Jieyu Shen, Danyang Wang, Graham L. Banes, Zhihua Zhang, Jianmin Wu, Xun Huang, Hua Chen, Siqin Ge, Chun-Long Chen, Yong E. Zhang
Summary: Transposable elements, previously regarded as junk, are now seen as catalysts of evolution. The analysis of 100 animal genomes reveals that terminal inverted repeat-type transposable elements catalyze new gene structures and genes in animals through both transposition-independent and -dependent mechanisms.
NATURE COMMUNICATIONS
(2021)
Article
Medicine, Research & Experimental
Julia Modesto Vicente, Caroline Honaiser Lescano, Silvana Bordin, Fabiola Zakia Monica, Gabriella Gobbi, Gabriel Forato Anhe
Summary: This study found that Agomelatine (Ago) can reduce platelet aggregation and adhesion, suggesting its potential in preventing atherothrombotic ischemic events.
Article
Pharmacology & Pharmacy
Corinna J. Mohr, Werner Schroth, Thomas E. Muerdter, Dominic Gross, Selina Maier, Benjamin Stegen, Alice Dragoi, Friederike A. Steudel, Severine Stehling, Reiner Hoppe, Stephen Madden, Peter Ruth, Stephan M. Huber, Hiltrud Brauch, Robert Lukowski
Summary: Pore-forming alpha subunits of the BK channel play a role in promoting breast cancer, while the absence or knockdown of BK subunits attenuate the proliferation of breast cancer cells. Tamoxifen stimulates the proliferation of breast cancer cells at low concentrations.
BRITISH JOURNAL OF PHARMACOLOGY
(2022)
Article
Cell & Tissue Engineering
Jennifer Steens, Lea Klar, Christine Hansel, Alexis Slama, Thomas Hager, Verena Jendrossek, Clemens Aigner, Diana Klein
Summary: Human lungs contain endogenous mesenchymal stem cells, with lung-resident MSCs predominantly located in the vascular stem cell niche of lung arteries. These LR-MSCs bear similarities to MSCs derived from adult human blood vessel walls, expressing a specific HOX code. The identification of LR-MSCs as VW-MSCs has implications for understanding lung physiology, pulmonary diseases, and therapeutic approaches.
STEM CELLS TRANSLATIONAL MEDICINE
(2021)
Article
Oncology
Alina Wittka, Julia Ketteler, Lars Borgards, Patrick Maier, Carsten Herskind, Verena Jendrossek, Diana Klein
Summary: In prostate cancer, inhibiting the gain of CAV1 in malignant epithelial cells and limiting the loss of stromal CAV1 are crucial for improving the response to radiotherapy. Additionally, targeting activated stromal fibroblasts by limiting fibroblast activation plays a key role in inhibiting the resistance-promoting CAV1-dependent signals of the tumor stroma.
FRONTIERS IN ONCOLOGY
(2022)
Article
Multidisciplinary Sciences
Matteo Rossi, Patricia Altea-Manzano, Margherita Demicco, Ginevra Doglioni, Laura Bornes, Marina Fukano, Anke Vandekeere, Alejandro M. Cuadros, Juan Fernandez-Garcia, Carla Riera-Domingo, Cristina Jauset, Melanie Planque, H. Furkan Alkan, David Nittner, Dongmei Zuo, Lindsay A. Broadfield, Sweta Parik, Antonino Alejandro Pane, Francesca Rizzollo, Gianmarco Rinaldi, Tao Zhang, Shao Thing Teoh, Arin B. Aurora, Panagiotis Karras, Ines Vermeire, Dorien Broekaert, Joke Van Elsen, Maximilian M. L. Knott, Martin F. Orth, Sofie Demeyer, Guy Eelen, Lacey E. Dobrolecki, Ayse Bassez, Thomas Van Brussel, Karl Sotlar, Michael T. Lewis, Harald Bartsch, Manfred Wuhrer, Peter van Veelen, Peter Carmeliet, Jan Cools, Sean J. Morrison, Jean-Christophe Marine, Diether Lambrechts, Massimiliano Mazzone, Gregory J. Hannon, Sophia Y. Lunt, Thomas G. P. Grunewald, Morag Park, Jacco van Rheenen, Sarah-Maria Fendt
Summary: The loss of phosphoglycerate dehydrogenase (PHGDH) has been found to increase cancer metastasis. Heterogeneous or low expression of PHGDH in primary tumors of breast cancer patients is associated with decreased metastasis-free survival time. Loss of PHGDH activates the hexosamine-sialic acid pathway through its interaction with the glycolytic enzyme phosphofructokinase, leading to increased cell migration and invasion.
Correction
Cell Biology
Violetta Ritter, Franziska Krautter, Diana Klein, Verena Jendrossek, Justine Rudner
CELL DEATH & DISEASE
(2022)
Article
Cell Biology
Luca Hegedues, Kata D. Szuecs, Matthias Kudla, Julian Heidenreich, Verena Jendrossek, Samuel Pena-Llopis, Tamas Garay, Andras Czirok, Clemens Aigner, Till Ploenes, Silvia Vega-Rubin-de-Celis, Balazs Hegedues
Summary: This study investigated the effect of Nintedanib on SRC kinase, one of its targets, in MPM cells. The results showed that Nintedanib did not inhibit SRC activation and even increased SRC phosphorylation in some MPM cell lines. However, combination treatment with the SRC inhibitor Dasatinib reversed this effect. The study also revealed that combination treatment promoted autophagy in MPM cells, which was independent of the AKT/mTOR and ERK pathways.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Kexu Xiang, Christian Kalthoff, Corinna Muench, Verena Jendrossek, Johann Matschke
Summary: Inhibition of SLC25A1 leads to accumulation of oncometabolite D-2-HG, increasing the sensitivity of cancer cells to radiotherapy and delaying DNA damage repair. Combining SLC25A1 inhibition with PARP or DNA-PKcs inhibitors further enhances radiotherapy-induced DNA damage and delays repair, resulting in increased radiosensitivity of cancer cells.
CELL DEATH & DISEASE
(2022)
Article
Oncology
Isabell Goetting, Safa Larafa, Katharina Eul, Mikhail Kunin, Burkhard Jakob, Johann Matschke, Verena Jendrossek
Summary: Aberrant activation of AKT affects cellular antioxidant defense and radiosensitivity. TRAMPC1 cells overexpressing the clinically relevant AKT variant AKT-E17K exhibit improved antioxidant defense and increased radiation resistance. This change is associated with AKT-dependent regulation of cellular levels of reduced glutathione (GSH), both directly and indirectly. Pharmacological inhibition of AKT-dependent metabolic enzymes supporting defense against oxidative stress, such as glutathione synthase and glutathione reductase, enhances the eradication of clonogenic tumor cells, particularly those overexpressing AKT-E17K.
FRONTIERS IN ONCOLOGY
(2022)
Correction
Multidisciplinary Sciences
Matteo Rossi, Patricia Altea-Manzano, Margherita Demicco, Ginevra Doglioni, Laura Bornes, Marina Fukano, Anke Vandekeere, Alejandro M. Cuadros, Juan Fernandez-Garcia, Carla Riera-Domingo, Cristina Jauset, Melanie Planque, H. Furkan Alkan, David Nittner, Dongmei Zuo, Lindsay A. Broadfield, Sweta Parik, Antonino Alejandro Pane, Francesca Rizzollo, Gianmarco Rinaldi, Tao Zhang, Shao Thing Teoh, Arin B. Aurora, Panagiotis Karras, Ines Vermeire, Dorien Broekaert, Joke Van Elsen, Maximilian M. L. Knott, Martin F. Orth, Sofie Demeyer, Guy Eelen, Lacey E. Dobrolecki, Ayse Bassez, Thomas Van Brussel, Karl Sotlar, Michael T. Lewis, Harald Bartsch, Manfred Wuhrer, Peter van Veelen, Peter Carmeliet, Jan Cools, Sean J. Morrison, Jean-Christophe Marine, Diether Lambrechts, Massimiliano Mazzone, Gregory J. Hannon, Sophia Y. Lunt, Thomas G. P. Grunewald, Morag Park, Jacco van Rheenen, Sarah-Maria Fendt
Article
Oncology
Sara Jennrich, Martin Pelzer, Tobias Tertel, Benjamin Koska, Melanie Vullings, Basant Kumar Thakur, Verena Jendrossek, Beate Timmermann, Bernd Giebel, Justine Rudner
Summary: Glioblastoma multiforme (GBM) is an aggressive tumor of the central nervous system with limited treatment options. Radiation therapy, specifically proton-based irradiation, shows promising results in treating GBM tumors. Extracellular vesicles (EVs) released by GBM cells can potentially serve as markers for predicting tumor response to radiotherapy.
FRONTIERS IN ONCOLOGY
(2022)
Article
Multidisciplinary Sciences
Christina Wenzek, Philine Steinbach, Florian Wirsdoerfer, Kathrin Sutter, Julia D. Boehme, Robert Geffers, Robert Klopfleisch, Dunja Bruder, Verena Jendrossek, Jan Buer, Astrid M. Westendorf, Torben Knuschke
Summary: CD47 plays a significant role in immune responses, but its role in antiviral immunity is not fully understood. This study revealed that CD47-deficient mice showed enhanced clearance of IAV, and this enhanced clearance was mediated by aM Phi. The study also found that CD47 restricts HB expression in aM Phi after IAV and SARS-CoV-2 infection, and HB enhances the IFN-b response, showing antiviral properties.
Article
Cell Biology
Jessica Morgner, Laura Bornes, Kerstin Hahn, Carmen Lopez-Iglesias, Lona Kroese, Colin E. J. Pritchard, Claire Vennin, Peter J. Peters, Ivo Huijbers, Jacco van Rheenen
Summary: This study reveals that the basement membrane (BM) surrounding mammary tumors has a faster turnover rate of laminin beta1 compared to the BM surrounding healthy epithelium. Cancer cells and tumor-infiltrating endothelial cells are found to synthesize laminin beta1, but its production is heterogeneous and temporary, leading to local discontinuity and even disappearance of the BM.
DEVELOPMENTAL CELL
(2023)
Article
Oncology
Nika Guberina, Florian Wirsdoerfer, Martin Stuschke, Verena Jendrossek
Summary: The risk of overlapping pulmonary toxicity induced by thoracic radio(chemo)therapy and immune checkpoint inhibitor therapy in the treatment of patients suffering from non-small cell lung cancer (NSCLC) is a significant challenge in successful radioimmunotherapy. Factors such as patient selection, monitoring biomarkers, and early detection of lung tissue changes play crucial roles in optimizing treatment outcomes and minimizing side effects. The concurrent use of radiotherapy and immunotherapy can provide new curative options for locally advanced NSCLC patients, but careful management of adverse events, especially in the lung, is necessary.
Article
Immunology
Kristina Zec, Stephanie Thiebes, Jenny Bottek, Devon Siemes, Philippa Spangenberg, Duc Viet Trieu, Nils Kirstein, Nirojah Subramaniam, Robin Christ, Diana Klein, Verena Jendrossek, Maria Loose, Florian Wagenlehner, Jadwiga Jablonska, Thilo Bracht, Barbara Sitek, Bettina Budeus, Ludger Klein-Hitpass, Dirk Theegarten, Olga Shevchuk, Daniel R. Engel
Summary: This study provides novel insights into the molecular adaptations of alveolar macrophages (AM) upon lung infection with Streptococcus pneumoniae. The results reveal a strong neutrophil-associated proteomic signature in AM, and identify CD11b as a central molecular hub influencing neutrophil recruitment, activation, and migration.
FRONTIERS IN IMMUNOLOGY
(2023)
Meeting Abstract
Immunology
C. Wenzek, P. Steinbach, F. Wirsdoerfer, K. Sutter, J. D. Boehme, R. Geffers, D. Bruder, V. Jendrossek, J. Buer, A. M. Westendorf, T. Knuschke
EUROPEAN JOURNAL OF IMMUNOLOGY
(2022)