Article
Chemistry, Applied
Chelsey Hinnenkamp, Baraem P. Ismail
Summary: The study hydrolyzed whey protein concentrate using trypsin and Protease M, showing that Protease M preferentially hydrolyzed alpha-lactalbumin while trypsin targeted beta-lactoglobulin. The two enzymes exhibited different specificity and produced peptides with varying hydrophobicity, but shared 9 bioactive peptides.
Article
Agriculture, Multidisciplinary
Minghao Zhang, Haiyue Sun, Yankai Liu, Yu Wang, Chunhong Piao, Dan Cai, Yuhua Wang, Jingsheng Liu
Summary: The study showed that an appropriate concentration of PUL could enhance the interactions between WPC and PUL, improving the gel by strengthening hydrogen bonding, electrostatic interactions, and exposure of hydrophobic groups, while excessive PUL inhibited the formation of disulfide bonds. Furthermore, interactions like hydrophobic interactions, disulfide bonds, and hydrogen bonds were affected under alkaline conditions. The rheological results indicated that both PUL concentration and pH had similar effects on the storage modulus of WPC/PUL gels.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2021)
Article
Agriculture, Dairy & Animal Science
G. Rathod, J. K. Amamcharla
Summary: Milk protein concentrate (MPC) is a valuable ingredient that can improve viscosity and consistency when the whey proteins are converted into fibrils. In this study, the fibrillated model MPC showed significantly higher viscosity and consistency coefficient compared with the control model MPC.
JOURNAL OF DAIRY SCIENCE
(2021)
Review
Physiology
Katie Harris, Jonathan Michael Laws, Antoine Elias, David Andrew Green, Nandu Goswami, Jens Jordan, Tovy Haber Kamine, Lucia Mazzolai, Lonnie G. Petersen, Andrew James Winnard, Tobias Weber
Summary: This review article discusses the need to predict the risk of venous thromboembolism in healthy individuals in space. The authors conducted a qualitative systematic review on endothelial disruption/dysfunction and identified some markers related to endothelial dysfunction. However, there is currently insufficient evidence to recommend the use of these new markers for screening.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
A. Swaminathan, M. S. Molitor, K. J. Burrington, D. Otter, J. A. Lucey
Summary: The study aimed to produce an enriched dairy lipid fraction from whey protein phospholipid concentrate (WPPC) using a nonsolvent-based separation process. Different chemical pretreatments were applied to the WPPC solutions to obtain proteins, fat, and phospholipids fractionated into layers with similar proportions. Protein composition was characterized through sodium dodecyl sulfate-PAGE under reducing and nonreducing conditions, showing various proteins present in each layer after centrifugation.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Chemistry, Applied
Quintin Ferraris, Armando Alcazar, Michael C. Qian
Summary: This study investigated the specific lipid species present in ethanol extracted lipid residues from commercially available WPPC. Sphingomyelins and phosphatidylcholines were found to be retained in the polar lipid enriched fraction, while some glycosphingolipids crystallized away from other polar lipids during fractionation. Correlation analysis supported that majority of polar lipids recovered using ethanol were retained in the polar lipid enriched residue.
Article
Acoustics
G. Genaro Amador-Espejo, I. Irving Ruiz-Lopez, J. Paola Gibbens-Bandala, J. Raul Delgado-Macuil, Hector Ruiz-Espinosa
Summary: The study evaluated the impact of thermosonication on producing reduced fat cheese as an alternative to full-fat cheese. By selecting appropriate conditions for cheese making and minimizing fat content and treatment duration, low-fat cheeses were successfully prepared with promising results in terms of quality and sensory acceptability.
ULTRASONICS SONOCHEMISTRY
(2021)
Article
Chemistry, Applied
Haiyue Sun, Minghao Zhang, Yankai Liu, Yu Wang, Yiying Chen, Wuyang Guan, Xia Li, Yuhua Wang
Summary: The study demonstrated that the addition of trehalose (TRE) can improve the viability of encapsulated L. plantarum, with optimal conditions being 5.0% TRE concentration and a 1:4 ratio. TRE reduced the storage modulus of the hydrogel and weakened interactions between proteins and polysaccharides, while also showing excellent water holding capacity.
CARBOHYDRATE POLYMERS
(2021)
Article
Chemistry, Applied
Tanja Z. Krunic, Marica B. Rakin
Summary: The study found that using trypsin hydrolysate in whey-based probiotic encapsulation can improve encapsulation efficiency and antioxidant capacity of the carrier. Hydrolysis of proteins positively impacts the beverage's antioxidant properties and probiotic viability, achieving the goals of protecting probiotics, enriching products with antioxidants, and neutralizing bitter taste from bioactive peptides.
Review
Nutrition & Dietetics
Fatemeh Hajizadeh-Sharafabad, Elham Sharifi Zahabi, Ali Tarighat-Esfanjani
Summary: Whey protein has a positive effect on flow-mediated dilation, but does not significantly impact arterial stiffness measures and circulatory biomarkers of vascular function. Further research is needed to confirm the benefits of whey protein on vascular function.
BRITISH JOURNAL OF NUTRITION
(2022)
Article
Polymer Science
Shuo Zhang, Guowei Deng, Fang Wang, Haiyan Xu, Jiagen Li, Jialei Liu, Dengfeng Wu, Shitao Lan
Summary: The study investigated the effect of preheated whey protein concentrate on the stability of purple sweet potato anthocyanins. It was found that whey protein concentrate can preserve the stability of anthocyanins by binding to them through non-covalent interaction.
Article
Food Science & Technology
Xueli Pu, Mengyuan Zhang, Xudong Lin, Yanfeng Tuo, Mian Safian Murad, Guangqing Mu, Shujuan Jiang
Summary: The gel prepared by mixing whey protein concentrate and egg white protein showed improved texture, water holding capacity, and thermal stability. Increasing EWP content led to enhanced hardness, adhesiveness, resilience, and gumminess in the composite gel, with EWP gel displaying the highest water holding capacity and the strongest resistance to deformation. Additionally, the rheological analysis indicated an increasing trend in storage modulus and loss modulus, with the DSC test showing the highest thermal stability in the WE1/1 gel.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Chemistry, Applied
Mina Kord Heydari, Elham Assadpour, Seid Mahdi Jafari, Hamedreza Javadian
Summary: This study aimed to optimize the preparation of whey protein concentrate (WPC)-pectin nanocomplexes as a carrier for rose essential oil (REO) using response surface methodology (RSM). The results showed that the highest encapsulation efficiency was achieved at a 4:0.5 ratio of WPC:pectin and pH = 3, while the highest stability was related to a 4:1 ratio of WPC:pectin at pH = 9. Additionally, the highest viscosity was obtained when using a 4:1 ratio of WPC:pectin and pH = 3, while the lowest stability was observed under the same conditions. Finally, the highest solubility was reported at pH = 9, with the lowest solubility seen at pH = 3 due to complex formation between WPC and pectin.
Article
Agriculture, Multidisciplinary
Matias Miguel Alancay, Manuel Oscar Lobo, Norma Cristina Samman
Summary: Protein-pectin conjugates obtained through controlled Maillard reaction have been studied for their contribution to improving emulsifying and thermal properties of proteins. The results showed that the formation of conjugates enhanced the emulsifying properties and improved the thermal stability of whey protein concentrate (WPC).
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2023)
Article
Biochemistry & Molecular Biology
Siyu Zhang, Cuina Wang, Weigang Zhong, Alyssa H. Kemp, Mingruo Guo, Adam Killpartrick
Summary: The study found that polymerized whey protein concentrate may serve as an effective carrier for enhancing the bioavailability and antioxidant capacity of GSH, with no significant toxic reactions observed.
Article
Nanoscience & Nanotechnology
Ewelina Piktel, Ilona Oscilowska, Lukasz Suprewicz, Joanna Depciuch, Natalia Marcinczyk, Ewa Chabielska, Przemyslaw Wolak, Tomasz Wollny, Marianna Janion, Magdalena Parlinska-Wojtan, Robert Bucki
Summary: Novel peanut-shaped gold nanoparticles (AuP NPs) were developed and shown to exhibit high anti-cancer activity against SKOV-3 cells in vitro. The AuP NPs decreased cell viability and proliferation by inducing apoptosis and autophagy, with overproduction of reactive oxygen species (ROS) playing a critical role in cell death.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2021)
Article
Cell Biology
Anna Gromotowicz-Poplawska, Piotr Szoka, Agnieszka Zakrzeska, Patrycjusz Kolodziejczyk, Natalia Marcinczyk, Janusz Szemraj, Piotr Tutka, Ewa Chabielska
Summary: The study found that aldosterone can enhance arterial thrombosis in rats, especially under diabetic and hyperglycemic conditions. Aldosterone shortens bleeding time, increases levels of tissue factor and plasminogen activator inhibitor in plasma, decreases levels of nitric oxide metabolites, and increases oxidative stress. Aldosterone induces a prothrombotic phenotype in endothelial cells, particularly under hyperglycemic conditions, by impacting primary hemostasis, the TF-dependent coagulation cascade, fibrinolysis, NO bioavailability, and oxidative stress balance.
Article
Biochemistry & Molecular Biology
Marta Baranowska-Kuczko, Hanna Kozlowska, Monika Kloza, Ewa Harasim-Symbor, Michal Biernacki, Irena Kasacka, Barbara Malinowska
Summary: Our study found that hypertension and chronic administration of the FAAH inhibitor URB597 have beneficial effects on the vascular endocannabinoid system in SHR, suggesting potential therapeutic benefits.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Natalia Marcinczyk, Agata Golaszewska, Anna Gromotowicz-Poplawska, Tomasz Misztal, Jakub Strawa, Michal Tomczyk, Irena Kasacka, Ewa Chabielska
Summary: The study demonstrated that tormentil extract (TE) had multidirectional effects on hemostasis in STZ-induced diabetic rats and mice. While TE inhibited platelet activity and improved endothelial functions, it also showed unfavorable effects by increasing the activity of the coagulation system and inhibiting fibrinolysis. These opposing effects may explain the model-specific influence of TE on the thrombotic process in STZ-induced diabetes.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Physiology
Agata Golaszewska, Tomasz Misztal, Natalia Marcinczyk, Ewa Chabielska, Tomasz Rusak
Summary: The study shows that adrenaline can enhance platelet activation and thrombus formation in the presence of subthreshold collagen, leading to increased platelet aggregation and formation of denser platelet-fibrin clots. These effects are associated with a decrease in fibrinolysis rate, which can be reduced by commonly used antiplatelet drugs.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Natalia Marcinczyk, Tomasz Misztal, Anna Gromotowicz-Poplawska, Agnieszka Zebrowska, Tomasz Rusak, Piotr Radziwon, Ewa Chabielska
Summary: The study introduced the PECAM-1/thrombus ratio as a parameter indicating platelet activation status, verified its usefulness in animal and human studies using a flow chamber model, and found that acetylsalicylic acid (ASA) could decrease thrombus area while increasing PECAM-1/thrombus ratio in a dose-dependent manner.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Anna Krzyzewska, Marta Baranowska-Kuczko, Krzysztof Minczuk, Hanna Kozlowska
Summary: There is currently no complete cure for pulmonary hypertension (PH), but research is being done on the potential use of cannabinoids as adjunctive therapy. Cannabinoids have vasodilatory effects on pulmonary circulation through various mechanisms, which may help reduce pulmonary vascular resistance and improve patients' quality of life.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Hanna Kozlowska, Barbara Malinowska, Marta Baranowska-Kuczko, Magdalena Kusaczuk, Milosz Nesterowicz, Miroslaw Kozlowski, Christa E. Mueller, Katarzyna Kiec-Kononowicz, Eberhard Schlicker
Summary: The GPR18 receptor protein, found in the heart and vasculature, plays a functional role in the cardiovascular system. New GPR18 agonists, PSB-MZ-1415 and PSB-MZ-1440, were shown to relax human pulmonary arteries, while the GPR18 antagonist PSB-CB-27 inhibited this effect. The activity of GPR18 appeared to be lower in male patients, smokers, and patients with hypercholesterolemia, potentially making it a new target for the treatment of pulmonary arterial hypertension.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Pharmacology & Pharmacy
Natalia Marcinczyk, Anna Gromotowicz-Poplawska, Michal Tomczyk, Ewa Chabielska
Summary: This paper describes the hemostasis-modifying ability of tannins, compounds of plant origin and plant extracts, and their influence on the thrombotic process. It highlights the effects of tannins on platelets, coagulation system, fibrinolysis system, and endothelium, as well as their pathophysiology-dependent action. The low bioavailability of tannins suggests the need for further in vivo studies to understand their actual activity.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Chemistry, Medicinal
Marta Baranowska-Kuczko, Hanna Kozlowska, Monika Kloza, Magdalena Kusaczuk, Ewa Harasim-Symbor, Michal Biernacki, Irena Kasacka, Barbara Malinowska
Summary: The study showed that chronic cannabidiol (CBD) treatment has vascular protective effects in hypertensive rats, improving endothelial function and vasodilation, partly through influencing the endocannabinoid system.
Article
Biochemistry & Molecular Biology
Anna Krzyzewska, Marta Baranowska-Kuczko, Anna Jastrzab, Irena Kasacka, Hanna Kozlowska
Summary: CBD has antioxidant and anti-inflammatory effects in the treatment of pulmonary hypertension. It can be used as an adjuvant therapy for pulmonary hypertension, but further research is needed to confirm its effectiveness.
Article
Nanoscience & Nanotechnology
Agnieszka M. Pudlarz, Katarzyna Ranoszek-Soliwoda, Michal S. Karbownik, Ewa Czechowska, Emilia Tomaszewska, Grzegorz Celichowski, Jaroslaw Grobelny, Ewa Chabielska, Anna Gromotowicz-Poplawska, Janusz Szemraj
Summary: This study found that the application of immobilized superoxide dismutase and catalase on nanoparticles can accelerate DNA repair and reduce harmful effects caused by UV radiation. The expression levels of nucleotide excision repair genes are increased with the application of immobilized enzymes.
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Justyna Rutkowska, Irena Kasacka, Marek Rogowski, Ewa Olszewska
Summary: This study aimed to determine the biological activity of cholesteatoma by analyzing the expression and localization of immunoproteasome subunits LMP2 and LMP7. The results showed the presence of LMP2 and LMP7 expressions within cholesteatoma tissue, providing new insights into immunoproteasome-dependent pathophysiologic mechanisms in cholesteatoma.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
