Article
Pharmacology & Pharmacy
Tao Wang, Li Wang, Yi Zhang, Jian Sun, Yilin Xie, Yan Yuan, Jianhong Gu, Jianchun Bian, Zongping Liu, Hui Zou
Summary: This study demonstrates that puerarin alleviates cadmium-induced hepatocyte injury by activating autophagy and mitigating autophagy blockade. Through investigating a cell model exposed to cadmium, it was found that puerarin enhanced autophagic activity, strengthened lysosomal degradation capacity, and restored the fusion of autophagosomes and lysosomes by upregulating Rab7 expression.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Food Science & Technology
Mengting Shang, Shuyan Niu, Xiaoru Chang, Jiangyan Li, Wenli Zhang, Menghao Guo, Tianshu Wu, Ting Zhang, Meng Tang, Yuying Xue
Summary: This study evaluated the effects of silver nanoparticles (AgNPs) on different stages of autophagy and found that they induced neuroinflammation and blocked autophagic flux. AgNPs hindered autophagic flux by inhibiting the fusion between autophagosomes and lysosomes, aggravating the neurotoxicity caused by AgNPs.
FOOD AND CHEMICAL TOXICOLOGY
(2022)
Review
Cell Biology
Xiaoyu Tian, Junlin Teng, Jianguo Chen
Summary: Macroautophagy/autophagy is a cellular mechanism for the degradation of cellular contents, with the final step being the fusion of autophagosome with the lysosome mediated by SNARE proteins. In addition to regulating autophagosome-lysosome fusion, some SNAREs are also involved in other autophagic processes, controlling fusion process spatially and temporally.
Article
Materials Science, Multidisciplinary
Yiming Geng, Shengyun Huang, Li Ma, Mingyang Li, Enli Yang, Yiming Li, Dongsheng Zhang, Xiao Fu, Haiwei Wu
Summary: Autophagy is a cellular process that captures and degrades intracellular components to maintain metabolism and homeostasis. In late stage tumorigenesis, autophagy promotes the survival, growth, and aggressiveness of tumors, as well as drug resistance. Nanoparticles can induce autophagy and offer a new perspective for tumor therapy strategies.
APPLIED MATERIALS TODAY
(2023)
Article
Cell Biology
Hansol Heo, Hyungsun Park, Myung Shin Lee, Jongyoon Kim, Juyeong Kim, Soon-Young Jung, Sun Kyeon Kim, Seongju Lee, Jaerak Chang
Summary: TRIM22 protein has been identified to play a novel role in autophagy regulation by mediating the association of GABARAP family proteins with PLEKHM1, thereby promoting autophagosome-lysosome fusion and facilitating autophagic clearance of protein aggregates. A TRIM22 variant associated with early-onset familial Alzheimer's disease interferes with autophagosome-lysosome fusion and autophagic clearance.
Article
Food Science & Technology
Xiaoxu Wang, Yu Song, Peixu Cong, Zhigao Wang, Yanjun Liu, Jie Xu, Changhu Xue
Summary: Docosahexaenoic acid-acylated astaxanthin monoester (AST-DHA) can rectify autophagic impairment in Alzheimer's disease and provide neuroprotection in Aβ-related pathology.
MOLECULAR NUTRITION & FOOD RESEARCH
(2023)
Review
Physiology
Shengyuan Wang, Hongyan Li, Minghao Yuan, Haixia Fan, Zhiyou Cai
Summary: Adenosine monophosphate-activated protein kinase (AMPK) plays a significant role in maintaining cellular energy homeostasis and is linked with different stages of autophagy.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Genetics & Heredity
Xiangli Zhao, Rossella Liberti, Jinlong Jian, Wenyu Fu, Aubryanna Hettinghouse, Ying Sun, Chuan-ju Liu
Summary: The study reveals that PGRN is a crucial mediator of autophagosome-lysosome fusion in Gaucher disease, and it interacts physically with Rab2, a molecule involved in this fusion process. A 15-kDa C-terminal fragment of PGRN is essential for binding to Rab2 and alleviating autophagic impairment caused by PGRN deficiency. These findings shed new light on autophagy mechanisms and potential therapeutic strategies for Gaucher disease.
JOURNAL OF MOLECULAR MEDICINE-JMM
(2021)
Article
Cell Biology
Zhifei Xu, Zezheng Pan, Ying Jin, Zizheng Gao, Feng Jiang, Huangxi Fu, Xueqin Chen, Xiaochen Zhang, Hao Yan, Xiaochun Yang, Bo Yang, Qiaojun He, Peihua Luo
Summary: This study revealed a novel mechanism for Crizotinib-induced cardiotoxicity, demonstrating that reviving the autophagy flux can reverse cardiomyocyte damage and mitochondrial injury, with metformin potentially serving as a therapeutic approach for Crizotinib-induced cardiotoxicity.
Article
Cell Biology
Hong Huang, Qinqin Ouyang, Kunrong Mei, Ting Liu, Qiming Sun, Wei Liu, Rong Liu
Summary: This study reveals that both acetylation and phosphorylation modifications control the function of SCFD1 in autophagosome-lysosome fusion. KAT2B/PCAF catalyzes the acetylation of SCFD1, while SIRT4 deacetylates it. Additionally, AMPK-controlled phosphorylation disrupts the interaction between SCFD1 and KAT2B and inhibits SCFD1 acetylation. Furthermore, SCFD1 acetylation inhibits autophagic flux by blocking SNARE complex formation.
Article
Cell Biology
Haitao Wang, Jianhua Zhang, Haoqiu Liu, Man Wang, Yan Dong, Yijun Zhou, Sek-Man Wong, Kai Xu, Qiufang Xu
Summary: The rice black-streaked dwarf virus (RBSDV) utilizes phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P-2) to evade autophagic degradation in insects and promote its survival.
Article
Biochemistry & Molecular Biology
Hao Wang, Guangxu Bai, Jianwei Chen, Wen Han, Ran Guo, Na Cui
Summary: The study found that during sepsis, the mTOR pathway modulates CD4 + T cell autophagy and apoptosis by regulating the transcription of A-L fusion-related proteins. Further experiments demonstrated that deletion of mTOR could alleviate fusion dysfunction between autophagosomes and lysosomes in CD4 + T cells, improving cell apoptosis.
Article
Biochemistry & Molecular Biology
Sumin Son, Ahruem Baek, Jong Hun Lee, Dong-Eun Kim
Summary: The study found that intermediate cytofilament keratin 8 (KRT8) interacts with the cytolinker plectin (PLEC) to facilitate autophagosome-lysosome fusion. Inhibition or disruption of this interaction attenuates the fusion process.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Cell Biology
Peili Hou, Xuefeng Wang, Hongmei Wang, Tiecheng Wang, Zhangping Yu, Chunqing Xu, Yudong Zhao, Wenqi Wang, Yong Zhao, Fengyun Chu, Huasong Chang, Hongchao Zhu, Jiahui Lu, Fuzhen Zhang, Xue Liang, Xingyu Li, Song Wang, Yuwei Gao, Hongbin He
Summary: This study demonstrates that SARS-CoV-2 infection activates the accumulation of autophagosomes and utilizes the autophagic machinery to promote viral replication. The ORF7a protein plays a crucial role in this process by initiating autophagy through specific molecular pathways and limiting the progression of autophagic flux, ultimately resulting in failure of autophagosome fusion with lysosomes.
Editorial Material
Cell Biology
Qinqin Ouyang, Rong Liu
Summary: The STX17-SNAP29-VAMP8 SNARE complex plays a role in the fusion between autophagosomes and lysosomes. This study shows that MTOR phosphorylates VAMP8, leading to the inhibition of autophagosome-lysosome fusion. It also identifies SCFD1 as a regulatory protein that promotes SNARE complex assembly and consequently enhances autophagosome-lysosome fusion. Furthermore, phosphorylation of VAMP8 disrupts autophagosome-lysosome fusion in the liver and affects lipid metabolism.
Article
Medicine, General & Internal
Jiawei Li, Yue Qiu, Long Li, Jiyan Wang, Yin Celeste Cheuk, Ruirui Sang, Yichen Jia, Jina Wang, Yi Zhang, Ruiming Rong
FRONTIERS IN MEDICINE
(2020)
Article
Pharmacology & Pharmacy
Dong Zhu, Qunye Tang, Baixue Yu, Mei Meng, Wenjie Liu, Jiawei Li, Tongyu Zhu, Paul M. Vanhoutte, Susan W. S. Leung, Yi Zhang, Yi Shi
BRITISH JOURNAL OF PHARMACOLOGY
(2020)
Article
Pharmacology & Pharmacy
Simeng Zhu, Shiqian Huang, Guofang Xia, Jin Wu, Yan Shen, Ying Wang, Rennolds S. Ostrom, Ailian Du, Chengxing Shen, Congfeng Xu
Summary: The study demonstrates that α7-nAChRs inhibit inflammation by activating adenylyl cyclase-6 and promoting degradation of TLR4, suggesting a novel therapeutic approach for treating COPD and other inflammatory diseases.
BRITISH JOURNAL OF PHARMACOLOGY
(2021)
Article
Immunology
Jiawei Li, Juntao Chen, Mingnan Zhang, Chao Zhang, Renyan Wu, Tianying Yang, Yue Qiu, Jingjing Liu, Tongyu Zhu, Yi Zhang, Ruiming Rong
Summary: The study found that mTOR deficiency enhances the immunosuppressive function of monocytic MDSCs and prolongs the survival time of mouse cardiac transplantation, mainly manifested as promoting MDSC differentiation, increasing intracellular autophagy levels, and inhibiting T cell activation and inducing regulatory T cells.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell Biology
Zhenyu Ma, Lulu Sheng, Juan Li, Jianmin Qian, Gang Wu, Zhengxin Wang, Yi Zhang
Summary: Resveratrol (RSV) exerts a protective effect in a CCl4-induced rat model of hepatic fibrosis (HF) by reducing collagen deposition, reversing epithelial-mesenchymal transition, and decreasing apoptosis and inflammation induced by endoplasmic reticulum stress (ERS).
Article
Immunology
Renyan Wu, Jiawei Li, Guowei Tu, Ying Su, Xuepeng Zhang, Zhe Luo, Ruiming Rong, Yi Zhang
Summary: This study analyzed RNA sequencing data to reveal the cellular and molecular features of fibrosis progression at different stages of AKI. It was found that T-cell activation mainly occurred in immune cells, while fatty acid metabolism and arachidonic acid metabolism happened in tubule cells.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell Biology
Yin Celeste Cheuk, Shihao Xu, Dong Zhu, Yongsheng Luo, Tian Chen, Juntao Chen, Jiawei Li, Yi Shi, Yi Zhang, Ruiming Rong
Summary: The supernatant derived from myeloid-derived suppressor cells (MDSCs) inhibits the transforming growth factor beta 1 (TGF-beta 1)-induced myofibroblastic differentiation of mesenchymal stem cells (MSCs) through IL-15. This finding provides a new perspective for the development of treatment strategies for renal fibrosis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Genetics & Heredity
Lulu Sheng, Yiqing Tong, Yi Zhang, Qiming Feng
Summary: This study investigated the gene interaction network associated with sepsis using weighted gene co-expression network analysis, identifying several gene modules with significant associations with sepsis. Further biological analysis revealed the promising roles of these genes in sepsis management.
FRONTIERS IN GENETICS
(2022)
Article
Cardiac & Cardiovascular Systems
Guo-wei Tu, Jie-fei Ma, Jia-kun Li, Ying Su, Jing-chao Luo, Guang-wei Hao, Ming-hao Luo, Yi-rui Cao, Yi Zhang, Zhe Luo
Summary: The miR-885-5p/HMBOX1 axis plays a significant role in septic myocardial depression. Sepsis-exos promote pyroptosis in AC16 cells through miR-885-5p, and HMBOX1 reverses this effect in an NF-kappa B-dependent manner.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Urology & Nephrology
Yamei Jiang, Chengzhe Cai, Pingbao Zhang, Yongsheng Luo, Jingjing Guo, Jiawei Li, Ruiming Rong, Yi Zhang, Tongyu Zhu
Summary: This study found that 18 beta-Glycyrrhetinic acid (GA) may alleviate renal fibrosis by inhibiting the inflammatory response. GA is a promising therapy that may be used in treating renal fibrosis and CKD.
Article
Cell Biology
Yin Celeste Cheuk, Xinhao Niu, Yongxin Mao, Jiawei Li, Jiyan Wang, Shihao Xu, Yongsheng Luo, Weixi Wang, Xuanchuan Wang, Yi Zhang, Ruiming Rong
Summary: This study uses an integrative approach combining transcriptomics and metabolomics to investigate the mechanisms underlying myofibroblastic differentiation of mesenchymal stem cells (MSCs). The results highlight the importance of glycolysis/gluconeogenesis and purine metabolism in this differentiation process. These findings provide valuable insights into the potential therapeutic targets for the treatment of fibrosis using myeloid-derived suppressor cell (MDSC) supernatant.
CELL AND TISSUE RESEARCH
(2022)
Article
Cell Biology
Shihao Xu, Yin Celeste Cheuk, Yichen Jia, Tian Chen, Juntao Chen, Yongsheng Luo, Yirui Cao, Jingjing Guo, Lijun Dong, Yi Zhang, Yi Shi, Ruiming Rong
Summary: This study found that mesenchymal stem cell-derived exosomes can significantly improve renal fibrosis by inhibiting glycolysis in tubular epithelial cells. miR-21a-5p repressed the expression of phosphofructokinase muscle isoform, thereby attenuating glycolysis. These findings provide new insights into the treatment of renal fibrosis.
CELL DEATH & DISEASE
(2022)
Article
Immunology
Guofang Xia, Hongyu Shi, Yuanyuan Su, Beibei Han, Chengxing Shen, Shiqiang Gao, Zhong Chen, Congfeng Xu
Summary: In this study, the researchers introduced photoactivated adenylyl cyclases into macrophages to regulate their pro-inflammatory response and investigate the effects on sepsis-induced myocardiopathy. By using a GelMA-Macrophages-LED system, they were able to inhibit inflammation and alleviate cardiac dysfunction in mice models of LPS-induced sepsis. This study represents a novel approach to treat sepsis-induced myocardiopathy and other cardiovascular diseases.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Cardiac & Cardiovascular Systems
Xin Meng, Guofang Xia, Lili Zhang, Congfeng Xu, Zhong Chen
Summary: This study investigated the expression profile of Tim-3 on CD4(+) and CD8(+) T cells in patients with acute decompensated heart failure (ADHF) and its impact on their prognosis. The results showed that Tim-3 expression on CD4(+) and CD8(+) T cells was significantly increased in the ADHF group compared to the non-ADHF group. High levels of Tim-3 expression on CD4(+) and CD8(+) T cells were found to be independent risk factors for ADHF and correlated with an increased risk of major adverse cardiac and cerebrovascular events (MACCE) within 12 months after ADHF.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Medicine, General & Internal
Yaping Zhang, Di Wang, Zhe Zhao, Liang Liu, Guofang Xia, Tianbao Ye, Yu Chen, Congfeng Xu, Xian Jin, Chengxing Shen
Summary: In this study, we found that overexpression of NPNT in mouse hearts can promote angiogenesis and improve cardiac function post myocardial infarction. NPNT treatment also enhances migration and tube formation of human umbilical vascular endothelial cells.
INTERNATIONAL JOURNAL OF MEDICAL SCIENCES
(2022)